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Effects of foundation damage and water-level change on vibration modal parameters of gravity-type caisson structure

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Science China(Technological Sciences) 2015年第2期58卷 316-329页

作者： LEE So-Young kim Jeong-tae Department of Ocean Engineering Pukyong National University

In this paper,the effects of foundation damage and water-level change on vibration characteristics of gravity-type caisson structure are examined by analyzing modal parameters extracted from output-only *** achieve the objective,the following approaches are ***,vibration response analysis methods are selected to estimate power spectral density and modal parameters such as natural frequency,damping ratio and mode shape of a lab-scale caisson structural ***,vibration tests on the lab-scale caisson system are performed under a series of test scenarios which include three water-level changes and three damage ***,experimental modal parameters corresponding to the damaging cases as well as the water level cases are extracted by frequency domain decomposition method and stochastic subspace identification ***,the effects of the water-level variation and foundation damage on the extracted modal parameters are examined to assess the feasibility of the vibration-based damage detection in gravity-type caisson structures under water-level uncertainty.

关键词： caisson structure gravity-type structure modal parameter effect of water-level foundation damage

Bright and stable near-infrared perovskite light emitters supported by multifunctional molecule design strategy

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Light(Science & Applications) 2023年第10期12卷 1995-1997页

作者： Joo Sung kim tae-Woo Lee SN Display Co.Ltd. SeoulRepublic of Korea Soft Foundry Seoul National UniversitySeoulRepublic of Korea Department of Materials Science and Engineering Research Institute of Advanced MaterialsSchool of Chemical and Biological EngineeringInstitute of Engineering ResearchSeoul National UniversitySeoulRepublic of Korea

Perovskite light emitters can realize bright,stable and efficient light-emitting diodes through a molecular design strategy that enables strong endurance on high-current operation.

关键词： emitter light diodes

Chemopreventive drugs:Mechanisms via inhibition of cancer stem cells in colorectal cancer

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World Journal of Gastroenterology 2014年第14期20卷 3835-3846页

作者： tae Il kim Department of Internal Medicine and Institute of Gastroenterology Yonsei University College of Medicine

Recent epidemiological studies,basic research and clinical trials on colorectal cancer(CRC)prevention have helped identify candidates for effective chemopreventive ***,because of the conflicting results of clinical trials or side effects,the effective use of chemopreventive drugs has not been generalized,except for patients with a high-risk for developing hereditary *** in genetic and molecular technologies have highlighted the greater complexity of carcinogenesis,especially the heterogeneity of *** need to target cells and processes that are critical to carcinogenesis for chemoprevention and treatment of advanced *** research has shown that intestinal stem cells may serve an important role in tumor initiation and formation of cancer stem ***,studies have shown that the tumor microenvironment may play additional roles in dedifferentiation,to enable tumor cells to take on stem cell features and promote the formation of tumorigenic stem ***,early tumorigenic changes of stem cells and signals for dedifferentiation may be good targets for *** this review,I focus on cancer stem cells in colorectal carcinogenesis and the effect of major chemopreventive drugs on stem cell-related pathways.

关键词： Colorectal cancer Chemoprevention Cancer stem cell Carcinogenesis Microenvironment

Comparative detection of syndecan-2 methylation in preoperative and postoperative stool DNA in patients with colorectal cancer

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World Journal of Gastrointestinal Surgery 2023年第9期15卷 2032-2041页

作者： Ji Hyeong Song tae Jeong Oh Sungwhan An kyung Ha Lee Ji Yeon kim Jin Soo kim Department of Surgery Chungnam National University Sejong HospitalSejong 30099South Korea Genomictree Inc.Daejeon 34027South Korea Department of Surgery College of MedicineChungnam National UniversityDaejeon 35015South Korea

BACKGROUND Early detection of colorectal cancer(CRC)is essential to reduce cancer-related morbidity and *** DNA(sDNA)testing is an emerging method for early CRC ***-2(SDC2)methylation is a potential biomarker for the sDNA *** DNA methylation is an early epigenetic event during tumorigenesis and can occur in the normal colonic mucosa during aging,which can compromise the sDNA test *** In this prospective study,we enrolled 151 patients with CRC who underwent curative surgical resection between September 2016 and May *** stool samples were collected from 123 patients and postoperative samples were collected from 122 patients.A total of 104 samples were collected from both preoperative and postoperative *** promoter methylation of SDC2 in sDNA was assessed using linear target enrichment quantitative methylation-specific real-time polymerase chain *** parameters were analyzed using the results of SDC2 *** Detection rates of SDC2 methylation in the preoperative and postoperative stool samples were 88.6%and 19.7%,*** tumor size(3 cm,P=0.019)and advanced T stage(T3–T4,P=0.033)were positively associated with the detection rate of SDC2 methylation before *** sex was associated with false positives after surgery(P=0.030).Cycle threshold(CT)values were significantly decreased postoperatively compared with preoperative values(P<0.001).The postoperative negative conversion rate for preoperatively methylated SDC2 was 79.3%(73/92).CONCLUSION Our results suggested that the SDC2 methylation test for sDNA has acceptable sensitivity and ***,small size and early T stage tumors are associated with a low detection rate of SDC2 *** the cycle threshold values significantly decreased after surgery,SDC2 methylation test for sDNA might have a diagnostic value for CRC.

关键词： Biomarkers DNA methylation Syndecan-2 Colorectal cancer

A simple, flexible and high-throughput cloning system for plant genome editing via CRISPR-Cas system

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Journal of Integrative Plant Biology 2016年第8期58卷 705-712页

作者： Hyeran kim Sang-tae kim Jahee Ryu Min kyung Choi Jiyeon Kweon Beum-Chang Kang Hyo-Min Ahn Suji Bae Jungeun kim Jin-Soo kim Sang-Gyu kim Center for Genome Engineering Institute for Basic Science Department of Chemistry Seoul National University

CRISPR-Cas9 system is now widely used to edit a target genome in animals and plants. Cas9 protein derived from Streptococcus pyogenes（Sp Cas9） cleaves double-stranded DNA targeted by a chimeric single-guide RNA（sg RNA）. For plant genome editing, Agrobacterium-mediated T-DNA transformation has been broadly used to express Cas9 proteins and sg RNAs under the control of Ca MV 35 S and U6/U3 promoter, respectively. We here developed a simple and high-throughput binary vector system to clone a 19 20 bp of sg RNA, which binds to the reverse complement of a target locus, in a large T-DNA binary vector containing an Sp Cas9 expressing cassette. Twostep cloning procedures：（1） annealing two target-specific oligonucleotides with overhangs specific to the Aar I restriction enzyme site of the binary vector; and（2） ligating the annealed oligonucleotides into the two Aar I sites of the vector, facilitate the high-throughput production of the positive clones. In addition, Cas9-coding sequence and U6/U3 promoter can be easily exchanged via the GatewayTMsystem and unique Eco RI/Xho I sites on the vector, respectively. We examined the mutation ratio and patterns when we transformed these constructs into Arabidopsis thaliana and a wild tobacco, Nicotiana attenuata. Our vector system will be useful to generate targeted large-scale knock-out lines of model as well as non-model plant.

关键词： Aar I-mediated sg RNA cloning CRISPR-Cas9 T-DNA binary vector Exchangeable U6/U3 promoter Gateway compatible Cas9 cloning

Preparation of patterned SiC and SiCN microstructures

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Science China(Technological Sciences) 2006年第2期49卷 164-171页

作者： SUNG In-kyung kim Dong-pyo Department of Fine Chemical Engineering and Chemistry of Chungnam National University TaejonSouth Korea 305-764

Patterned SiC and SiCN microstructures were successfully fabricated on the silicon substrates by using polydimethylsiloxane (PDMS) elastometric stamp as template, polycarbosilane (PCS) and polysilazane (PSZ) as preceramic polymers. The preparing process was followed by precursor infiltration, the curing of the precursor, demolding of the template and pyrolysis of the cured preceramic polymer pattern. It shows that the dimen- sions of the ceramic patterns can be tailored by using the PDMS molds with different di- mensions. The produced ceramic microstructures can be potentially applied in high tem- perature and high pressure environments due to the advanced properties of the SiC and SiCN ceramics.

关键词： micro-fabrication technique, soft lithography, elastomeric stamp, non-oxide ceramics, patterning.

Programmed death ligand-1 expression and its prognostic role in esophageal squamous cell carcinoma

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World Journal of Gastroenterology 2016年第37期22卷 8389-8397页

作者： Ryul kim Bhumsuk Keam Dohee Kwon Chan-Young Ock Miso kim tae Min kim Hak Jae kim Yoon kyung Jeon In Kyu Park Chang Hyun Kang Dong-Wan kim Young tae kim Dae Seog Heo Department of Internal Medicine Seoul National University HospitalSeoul 03080South Korea Cancer Research Institute Seoul National University College of MedicineSeoul 03080South Korea Department of Pathology Seoul National University HospitalSeoul 03080South Korea Department of Radiation Oncology Seoul National University HospitalSeoul 03080South Korea Department of Thoracic and Cardiovascular Surgery Seoul National University HospitalSeoul 03080South Korea

AIM To investigate the expression and prognostic role of programmed death ligand-1(PD-L1) in locally advanced esophageal squamous cell carcinoma(ESCC).METHODS A total of 200 patients with ESCC who underwent radical esophagectomy with standard lymphadenectomy as the initial definitive treatment in Seoul National University Hospital from December 2000 to April 2013 were eligible for this analysis. Tissue microarrays were constructed by collecting tissue cores from surgical specimens, and immunostained with antibodies directed against PD-L1, p16, and c-Met. Medical records were reviewed retrospectively to assess clinical outcomes. Patients were divided into two groups by PD-L1 status, and significant differences in clinicopathologic characteristics between the two groups were assessed. RESULTS Tumor tissues from 67 ESCC patients(33.5%) were PDL1-positive. Positive p16 expression was observed in 21 specimens(10.5%). The H-score for c-Met expression was ≥ 50 in 42 specimens(21.0%). Although PDL1-positivity was not significantly correlated with any clinical characteristics including age, sex, smoking/alcoholic history, stage, or differentiation, H-scores for c-Met expression were significantly associated with PDL1-positivity(OR = 2.34, 95%CI: 1.16-4.72, P = 0.017). PD-L1 expression was not significantly associated with a change in overall survival(P = 0.656). In contrast, the locoregional relapse rate tended to increase(P = 0.134), and the distant metastasis rate was significantly increased(HR = 1.72, 95%CI: 1.01-2.79, P = 0.028) in patients with PD-L1-positive ESCC compared to those with PD-L1-negative *** PD-L1 expression is positively correlated with c-Met expression in ESCC. PD-L1 may play a critical role in distant failure and progression of ESCC.

关键词： Esophageal neoplasm Programmed death ligand-1 protein c-Met protein Prognosis p16INK4A protein

Arabidopsis thaliana RECEPTOR DEAD KINASE1 Functions as a Positive Regulator in Plant Responses to ABA

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Molecular Plant 2017年第2期10卷 223-243页

作者： Dhinesh Kumar Ritesh Kumar Dongwon Baek tae-kyung Hyun Woo Sik Chung Dae-Jin Yun Jae-Yean kim Division of Applied Life Sciences Plant Molecular Biology and Biotechnology Research Center Gyeongsang National University Jinju 660-701 Korea Department of Industrial Plant Science and Technology Conege of Agricultural Life and Environmental Sciences Chungbuk National University Cheongju 28644Korea These authors contributed equally to this article.

Abscisic acid （ABA） is a major phytohormone involved in important stress-related and developmental plant processes. Membrane-delimited ABA signal transduction plays an important role in early ABA signaling, but the molecular mechanisms connecting core signaling components to the plasma membrane remain un- clear. Plants have evolved a large number of receptor-like kinases （RLKs） to modulate diverse biological processes by perceiving extracellular stimuli and activating downstream signaling responses. In this study, a putative leucine-rich repeat-RLK gene named RECEPTOR DEAD KINASE1 （AtRDK1） was identified and characterized in Arabidopsis thaliana. RDK1 promoter-GUS analysis revealed that RDK1 is expressed ubiq- uitously in the various tissues in Arabidopsis, and its expression is mainly induced by ABA. In the presence of ABA, RDKl-deficient rdkl-1 and rdkl-2 lines showed significant resistance in cotyledon greening and root growth, whereas RDKl-overexpressing lines showed enhanced sensitivity. Consistently, the expres- sion of ABA-responsive genes was significantly downregulated in rdkl mutant seedlings, which were also hypersensitive to drought stress with increased water loss. Interestingly, RDK1 was found to be an atypical kinase localized to the plasma membrane and did not require its kinase activity during ABA-mediated inhi- bition of seedling development. Accordingly, RDK1 interacted in the plasma membrane with type 2C protein phosphatase ABSClSIC ACID INSENSITIVE1 （ABI1）; this interaction was further enhanced by exogenous application of ABA, suggesting that RDKl-mediated recruitment of ABI1 onto the plasma membrane is important for ABA signaling. Taken together, these results reveal an important role for RDK1 in plant responses to abiotic stress conditions in an ABA-dependent manner.

关键词： ABA abiotic stress Arabidopsis atypical kinases protein phosphatases receptor-like kinases

Ultimate strength of single shear bolted connections with cold-formed ferritic stainless steel

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2013年第2期14卷 120-136页

作者： Jin-seong LIM tae-soo kim Seung-hun kim Department of Architectural Engineering Hanbat National UniversityDaejeon 305-719Korea

This paper is focused on the structural behavior of the single shear bolted connections with thin-walled ferritic stainless *** purpose of this study is to investigate the ultimate behaviors,such as ultimate strength and fracture mode of the single shear bolted connections of thin-walled ferritic stainless steel(low cost steel) rather than austenitic stainless steel(high cost steel).Bolt arrangement and end distance parallel to the direction of applied load are considered as main variables of the test specimens for bolted *** have a constant dimension of edge distance perpendicular to the loading direction,bolt diameter,pitch,and gauge.A monotonic tensile test for specimens has been carried out and some bolted connections with long end distance showed curling(out of plane deformation) occurrence which led to strength *** ultimate behaviors such as fracture mode,ultimate strength are compared with those predicted by current design ***,conditions of curling occurrence and the strength reduction due to curling are investigated and modified strength equations are suggested considering the curling effect.

关键词： Cold-formed ferritic stainless steel Bolted connection Bolt arrangement End distance Curling Strength reduction