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Extract of Emblica officinalis enhances the growth of human keratinocytes in culture

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Journal of Integrative Medicine 2019年第2期17卷 141-146页

作者： Yoshimi Yamakami kyoko Morino Yuki Takauji Ryuichiro Kasukabe Kensuke Miki Mohammad Nazir Hossain Dai Ayusawa Michihiko Fujii Graduate School of Nanobioscience Yokohama City University Ichiban Life Corporation Department of Biochemistry Primeasia University

Objective: Keratinocytes are the predominant cell type in the epidermis and play key roles in epidermal function. Thus, identification of the compounds that regulate the growth of keratinocytes is of importance. Here we searched for such compounds from the herbs used in traditional medicine ***: Human keratinocytes were cultured in the presence or absence of the herbal extracts for 2 weeks; the effect of the extracts on cell growth was determined by staining the cells with Coomassie brilliant blue. To detect the compounds that regulate the growth of keratinocytes, the herbal extracts were subjected to high-performance liquid chromatography(HPLC).Results: We found that the extract of Emblica officinalis enhanced the growth of keratinocytes in ***, we fractionated the extract of E. officinalis using HPLC and identified the fractions responsible for the enhanced growth of ***: The extract of E. officinalis enhanced the growth of human keratinocytes in culture.E. officinalis contains the compounds that would be beneficial for human skin health because enhanced growth of keratinocytes would promote wound healing.

关键词： Keratinocyte Cell growth Ayurveda Emblica officinalis

Ultra-large-scale phase-field simulation study of ideal grain growth

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npj Computational Materials 2017年第1期3卷 247-252页

作者： Eisuke Miyoshi Tomohiro Takaki Munekazu ohno Yasushi Shibuta Shinji Sakane Takashi Shimokawabe Takayuki Aoki Graduate School of Science and Technology Kyoto Institute of TechnologyMatsugasakiSakyo-kuKyoto 606-8585Japan Faculty of Mechanical Engineering Kyoto Institute of TechnologyMatsugasakiSakyo-kuKyoto 606-8585Japan Division of Materials Science and Engineering Faculty of EngineeringHokkaido UniversityKita 13 Nishi 8Kita-kuSapporoHokkaido 060-8628Japan Department of Materials Engineering The University of Tokyo7-3-1 HongoBunkyo-kuTokyo 113-8656Japan The Supercomputing Division Information Technology CenterThe University of Tokyo2-11-16 YayoiBunkyo-kuTokyo 113-8658Japan Global Scientific Information and Computing Center Tokyo Institute of Technology2-12-1 i7-3 O-okayamaMeguro-kuTokyo 152-8550Japan

Grain growth,a competitive growth of crystal grains accompanied by curvature-driven boundary migration,is one of the most fundamental phenomena in the context of metallurgy and other scientific ***,the true picture of grain growth is still controversial,even for the simplest(or‘ideal’)*** problem can be addressed only by large-scale numerical ***,we analyze ideal grain growth via ultra-large-scale phase-field simulations on a supercomputer for elucidating the corresponding authentic statistical *** performed simulations are more than ten times larger in time and space than the ones previously considered as the largest;this computational scale gives a strong indication of the achievement of true steady-state growth with statistically sufficient number of ***,we provide a comprehensive theoretical description of ideal grain growth behaviors correctly quantified by the present *** findings provide conclusive knowledge on ideal grain growth,establishing a platform for studying more realistic growth processes.

关键词： grain field establishing

Molecular Phylogeny and Evolution of Two Rhacophorus Species Endemic to Mainland Japan

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Asian Herpetological Research 2019年第2期10卷 86-104页

作者： Masafumi matsui Yasuhiro KAWAHARA Kanto NISHIKAWA Seiji IKEDA Koshiro ETO Yusuke MIZUNO Graduate School of Human and Environmental Studies Kyoto UniversityYoshida Nihonmatsu-choSakyo-kuKyoto 606-8501Japan Hagi City Museum Horiuchi 355HagiYamaguchi 758-0057Japan Hiroshima University Museum Kagamiyama 1-1-1Higashi Hiroshima CityHiroshima 739-8524Japan Kitakyushu Museum of Natural History and Human History Higashida 2-4-1Yahatahigashi-kuKitakyushuFukuoka 805-0071Japan Takahari 4-1002 Meito-kuNagoya 465-0061Japan

We conducted molecular phylogenetic analyses of Japanese Rhacophorus species, especially of R. schlegelii and R. arboreus from the mainland, based on samples encompassing their known distribution ranges, and discussed about evolutionary history of Rhacophorus species within Japan. The common ancestor of Japanese Rhacophorus, except for R. owstoni from southern Ryukyus, was estimated to have diverged from a lineage occurring mainly in China about 7 MYBP. Both R. schlegelii and R. arboreus are genetically largely divergent between regions of eastern and western Japan, and this seems to have been promoted mainly by retreat to refugia. Retreats of the two species to different refugia sometimes in the past seem to have led restricted distribution of R. schlegelii in eastern and R. arboreus in western Japan, and brought their intraspecific variation patterns in morphology and breeding habit.

关键词： glacial period mitochondrial DNA differentiation refugia Rhacophorus arboreus Rhacophorus schlegelii

Anaplastic carcinoma of the pancreas: Case report and literature review of reported cases in Japan

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World Journal of Gastroenterology 2016年第38期22卷 8631-8637页

作者： Sojun Hoshimoto Junichi matsui Ryohei Miyata Yutaka Takigawa Jun Miyauchi Department of Surgery Tokyo Dental College Ichikawa General Hospital Department of Pathology Tokyo Dental College Ichikawa General Hospital

We report a case of a 64-year-old woman with anaplastic carcinoma of the pancreas(ACP) with cyst formation and review 60 ACP cases reported in Japan. In 20% of cases, laboratory tests revealed severe anemia(hemoglobin level 12000/mm3), which were likely attributable to rapid tumor growth, intratumoral hemorrhage, and necrosis. Elevated serum CA19-9 levels were observed in 55% of cases. Cyst-like structures were observed on imaging in 47% of cases, and this finding appears to reflect subsequent cystic degeneration in the lesion. Macroscopically, hemorrhagic necrosis was observed in 77% of cases, and cyst formation was observed in 33% of cases. ACP should be considered when diagnosing pancreatic tumors with a cyst-like appearance, especially in the presence of severe anemia, elevated leucocyte counts, or elevated serum CA19-9 levels.

关键词： Anaplastic carcinoma Pancreatic cancer Pleomorphic type Prognosis Undifferentiated carcinoma

F213I突变型高雪氏病分子伴侣治疗方法的研究

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中华医学遗传学杂志 2003年第5期20卷 381-384页

作者：侯琳 ohno Kousaku 青岛大学医学院生化教研室 266021 日本鸟取大学医学部神经生物教室

目的　研究高雪氏病新的分子治疗方法。方法　用β-葡萄糖脑苷脂酶 (β-glucocerebrosidase,β-Glc) (EC3 .2 .1.45)的底物类似物 (glucocerebroside analogue,GCA)作为分子伴侣 ,处理体外培养的不同突变型的高雪氏病患者皮肤成纤维细... 详细信息

目的　研究高雪氏病新的分子治疗方法。方法　用β-葡萄糖脑苷脂酶 (β-glucocerebrosidase,β-Glc) (EC3 .2 .1.45)的底物类似物 (glucocerebroside analogue,GCA)作为分子伴侣 ,处理体外培养的不同突变型的高雪氏病患者皮肤成纤维细胞 ,采用荧光酶学手段测定β-Glc活性 ;Western印迹杂交技术分析该酶蛋白表达的量 ;细胞双染确定 β-Glc在细胞内的定位 ;薄层层析法分析葡萄糖脑苷脂的降解情况。结果该酶的底物类似物 GCA可以提高体外培养的 F2 13 I突变型患者成纤维细胞内 β-Glc活性 ;增加该酶蛋白的表达量 ;促进该酶蛋白向溶酶体内转运 ;加速底物葡萄糖脑苷脂 (glucocerebroside,Glc Cer)的降解。结论　低分子量的 GCA作为一种分子伴侣可能为 F2 13

关键词：高雪氏病治疗分子伴侣基因突变 β-葡萄糖脑苷脂酶 β-GLe

Application of highwall mining system in weak geological condition

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International Journal of Coal Science & Technology 2016年第3期3卷 311-321页

作者： Takashi Sasaoka Tri Karian Akihiro Hamanaka Hideki Shimada Kikuo matsui Department of Earth Resources Engineering KyushuUniversity Fukuoka 819-0395 Japan

Almost all the coal is produced from open cut mines in Indonesia. As a consequence of open cut mine application, a great deal of coal is left out in the highwalls of the mined-out pits. Highwall mining systems can be used to recover this coal. The use of highwall mining systems has increasingly come into play in the US and Australia. However, it is not common in Indonesia. Moreover, Indonesia coal measure is categorized as weak geological condition. Some problems are likely to arise during the application of the highwall mining system for example instability of openings and highwalls due to the roof and pillar failures. Therefore, study of highwall mining system application in Indonesia is needed in order to increase the recovery rate of coal mining in Indonesia. This paper described the characteristics of the highwa!l mining system and discussed the appropriate highwall mining system application in weak geological condition, Indonesia. From the results of a series of laboratory tests and numerical analyses, it can be concluded that the stability of pillars and mine openings in auger mining systems is much higher than that in CHM and an auger mining system is suitable for such as very weak/poor strata conditions. Moreover, the application of backfilling system is very effective for improvement of the stability of pillar and openings.

关键词： Open cut mine Left out coal Highwall mining systems Weak geological condition

Chyawanprash,a formulation of traditional Ayurvedic medicine,shows a protective effect on skin photoaging in hairless mice

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Journal of Integrative Medicine 2016年第6期14卷 473-479页

作者： Yuki Takauji kyoko Morino Kensuke Miki Mohammad Hossain Dai Ayusawa Michihiko Fujii Graduate School of Nanobioscience Yokohama City UniversityKanagawa 236-0027Japan Department of Biochemistry Primeasia UniversityDhaka 1213Bangladesh Ichiban Life Corporation 1-1-7 Horai-choNaka-kuYokohama 231-0048Japan

OBJECTIVE： Chronic exposure to ultraviolet （UV） radiation induces skin photoaging （premature skin aging）. UV irradiation generates reactive oxygen species （ROS）, which are shown to play a pivotal role in skin photoaging. Ayurveda is a holistic traditional medical system, and Chyawanprash is one of the most popular formulations in Ayurveda. Since maintenance of the function and appearance of skin is important, we examined whether Chyawanprash has a protective effect on skin photoaging. METHODS： To examine the effect of Chyawanprash on skin photoaging, hairless mice were administered with Chyawanprash in drinking water for 3 weeks, and then repeatedly exposed to ultraviolet light B （UVB） irradiation （225 or 450 mJ/cm2） to induce skin photoaging. To further examine the function of Chyawanprash, its effects were examined in cells cultured in vitro. Chyawanprash was added in culture medium, and examined for the effect on the growth of human keratinocytes, and for the ability to eliminate ROS which generated by paraquat （50 μmol/L） in HeLa cells. RESULTS： UVB irradiation caused symptoms such as rough skin, erythema, and edema on the skin in hairless mice, but administration of Chyawanprash relieved these symptoms. Further, Chyawanprash significantly suppressed epidermal thickening, a typical marker of skin photoaging, in mice. We then analyzed the effect of Chyawanprash in human cells in culture, and found that Chyawanprash enhanced the growth of human keratinocytes, and efficiently eliminated ROS, which are causally involved in skin photoaging, in HeLa cells. CONCLUSION： These findings suggested that Chyawanprash may have beneficial effects on slowing skin photoaging.

关键词： medicine, Ayurvedic Chyawanprash photoaging of skin reactive oxygen species

Preparation of luminescent polystyrene microspheres via surface-modified route with rare earth(Eu^(3+) and Tb^(3+))complexes linked to 2,2'-bipyridine

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Rare Metals 2015年第8期34卷 590-594页

作者： Zhen-Xiong Ma Qi-Tao Zhang Jun-Liang Liu Chang-Hao Yan Ming Zhang Teruhisa ohno School of Chemistry and Chemical Engineering Yangzhou University Department of Applied Chemistry Faculty of EngineeringKyushu Institute of Technology School of Chemical Engineering Yangzhou Polytechnic Institute

Luminescent polystyrene microspheres were easily fabricated from poly(styrene-co-methacrylic acid)and aqueous RE(III) chloride solution(RE=Eu, Tb) in the presence of 2,20-bipyridine as second ligand. The negative charges of carboxyl groups on the surface of microspheres coordinated with rare earth ions at first, such as complexes covalently linked to 2,20-bipyridine, resulting in strong photoluminescence. Various methods, including transmission electron microscope(TEM), scanning electron microscope(SEM), energy dispersive spectroscopy(EDS),Fourier transform infrared spectroscopy(FT-IR), and fluorescence spectrophotometer, were used to characterize the resultant polystyrene composite microspheres. This work highlights the idea that it is facile to synthesis luminescent microspheres by surface-modified method directly.

关键词： Luminescent Polystyrene Terbium Europium 2,2’-bipy

GM_2神经节苷脂沉积症发病的分子机理研究

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中华医学遗传学杂志 2003年第2期20卷 103-106页

作者：侯琳 ohno Kousaku 青岛大学医学院生化教研室 266021 日本鸟取大学医学部神经生物教室

目的　探讨GM2 神经节苷脂沉积症发病的分子机理。方法　培养患者皮肤成纤维细胞 ,进行酶学、Western印迹杂交和免疫细胞化学分析。结果　 4例GM2 神经节苷脂沉积症患者成纤维细胞内 β 氨基己糖苷酶 (hexosaminidase ,Hex)活性均显著降... 详细信息

目的　探讨GM2 神经节苷脂沉积症发病的分子机理。方法　培养患者皮肤成纤维细胞 ,进行酶学、Western印迹杂交和免疫细胞化学分析。结果　 4例GM2 神经节苷脂沉积症患者成纤维细胞内 β 氨基己糖苷酶 (hexosaminidase ,Hex)活性均显著降低 ,分别为正常人的 12 %、3 %、15 %和 6% ;两例Sandhoff病Hex成熟的α、β 亚基 (αm、βm)明显减少 ,但婴儿型与成年型减少的程度不同 ,1例Tay Sachs病只有αm明显减少 ,1例GM2 激活蛋白缺陷症αm、βm与正常人无差异 ;4例患者成纤维细胞内均有不同程度的GM2神经节苷脂的堆积。结论　GM2 神经节苷脂沉积症的发病与HEXA、HEXB、GM2 A基因突变引起相应蛋白表达、成熟障碍有关。

关键词： GM2神经节苷脂沉积症 β-氨基已糖苷酶亚基