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Analysis of Carbon Functional Groups in Mobile Humic Acid and Recalcitrant Calcium Humate Extracted from Eight US Soils

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Pedosphere 2013年第6期23卷 705-716页

作者： HE Zhong-Qi CAO Xiao-Yan MAO Jing-Dong T.ohno H.M.WALDRIP USDA-ARS Southern Regional Research Center Department of Chemistry and Biochemistry Old Dominion University Department of Plant Soiland Environmental SciencesUniversity of Maine USDA-ARS Conservation and Production Research Laboratory

Solid state 13C nuclear magnetic resonance （NMR） spectroscopy is a common tool to study the structure of soil humic fractions; however, knowledge regarding carbon structural relationships in humic fractions is limited. In this study, mobile humic acid （MHA） and recalcitrant calcium humate （CallA） fractions were extracted from eight soils collected from six US states and representing a variety of soils and ecoregions, characterized by this spectroscopic technique and analyzed for statistical significance at P 〈 0.05. We found that the abundances of COO and N-C=O functional groups in the MHA fractions were negatively correlated to soil sand content, but were positively correlated to silt, total N and soil organic carbon contents. In contrast, the abundances of the COO and N-C=O functional groups were only positively correlated to the content of clay in the CallA fractions, indicating that the two humic fractions were associated with different soil components. The two 13C NMR peaks representing alkyls and OCH3/NCH were negatively correlated to the peaks representing aromatics, aromatic C-O and N-C=O/COO. Comparison of the sets of data from 13C NMR spectroscopy and ultrahigh resolution mass spectrometry revealed that the aromatic components identified by the two methods were highly consistent. The comparison further revealed that protein in MHA was associated with, or bound to, the nonpolar alkyl groups, but a component competitively against （or complementary to） aromatic groups in the MHA composition. These observations provided insight on the internal correlations of the functional groups of soil humic fractions.

关键词： correlation analysis humic substances nuclear magnetic resonance soil organic matter ultrahigh resolution mass spectrometry

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Overexpression of INCREASED CAMBIAL ACTIVITY, a putative methyltransferase, increases cambial activity and plant growth

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Journal of Integrative Plant Biology 2016年第11期58卷 874-889页

作者： Hyunsook Kim Mikiko Kojima Daeseok Choi Soyoung Park Minami matsui Hitoshi Sakakibara Ildoo Hwang Department of Life Sciences Pohang University of Science and Technology Pohang 790-784 Korea Synthetic Genomics Research Team Biomass Research Cooperation Division (BMEP) RIKEN Center for Sustainable Resource Science (CSRS) Tsurumi Yokohama 230-0045 Japan Riken Center for Sustainable Resource Science (CSRS) Tsurumi Yokohama 230-0045 Japan

Cambial activity is a prerequisite for secondary growth in plants; however, regulatory factors controlling the activity of the secondary meristem in radial growth remain elusive. Here, we identified INCREASED CAMBIAL ACTIVITY （ICA）, a gene encoding a putative pectin methyltransferase, which could function as a modulator for the meristematic activity of fascicular and interfascicular cambium in Arabidopsis. An overexpressing transgenic line, 35S：＇1CA, showed accelerated stern elongation and radial thickening, resulting in increased accumulation of biomass, and increased levels of cytokinins （CKs） and gibberellins （GAs）. Expression of genes encoding pectin methylesterases involved in pectin modification together with pectin methyltransferases was highly induced in 355：：ICA, which might contribute to an increase of methanol emission as a byproduct in 35S：ICA. Methanol treatment induced the expression of GA- or CK-responsive genes and stimulated plant growth. Overall, we propose that ectopic expression of ICA increases cambial activity by regulating CK and GA homeostasis, and methanol emission, eventually leading to stem elongation and radial growth in the inflorescence stem.

关键词： Cambial activity cytokinin gibberellin methanol methyltransferase pectin shoot growth

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Median neuropathy after multiple punctures of the forearm for catheterization:A case report

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世界临床病例杂志 2023年第25期11卷 5941-5946页

作者： Tomoaki Suzuki Yuichiro matsui Daisuke Momma Takeshi Endo Norimasa Iwasaki Department of Orthopaedic Surgery Faculty of Medicine and Graduate School of MedicineHokkaido UniversitySapporo 060-8638Japan Section for Clinical Education Faculty of Dental MedicineHokkaido UniversitySapporo 060-8586Japan Center for Sports Medicine Hokkaido UniversitySapporo 060-8638Japan

BACKGROUND Neuropathy may occur at some sites after catheterization for close examination of cardiac *** the radial artery is considered a relatively uncomplicated site for catheterization,the radial artery and median nerve are in relatively close proximity,with the risk of median nerve injury depending on the angle of *** purpose of this study was to report the outcomes of surgery performed for conservative therapy-resistant median neuropathy following forearm *** SUMMARY A 50-year-old woman experienced palsy from the right thumb to the radial side of the ring finger after catheterization from the radial artery of the right *** developed at the same site and a positive tinel-like sign was seen for the median nerve area at the high level of the puncture *** conduction study showed reduced compound muscle action potentials and loss of sensory nerve action *** did not improve despite pharmacotherapy and the patient gradually developed flexion restrictions of the index and middle *** nerve injury and associated flexor tendon adhesion was diagnosed,and the patient was referred for surgery at 3 mo after *** the same area was opened,no injury to the median nerve epithelium was obvious,but the area of the positive tinel-like sign was highly adherent to surrounding tissue and to the flexor digitorum superficialis of the index and middle *** surgery was terminated with adequate adhesion *** was initiated postoperatively,improving neurological symptoms and range of motion of the *** months after surgery,the patient returned to daily activities without *** This case provides the appropriate diagnosis and treatment for a suspected peripheral nerve injury.

关键词： Catheterization Median neuropathy Surgical treatment Case report

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Update on gut microbiota in gastrointestinal diseases

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World Journal of Clinical Cases 2022年第22期10卷 7653-7664页

作者： Atsushi Nishida Kyohei Nishino Masashi ohno Keitaro Sakai Yuji Owaki Yoshika Noda Hirotsugu Imaeda Department of Gastroenterology and Hepatology Nagahama City HospitalNagahama 526-8580Japan

The human gut is a complex microbial ecosystem comprising approximately 100 trillion microbes collectively known as the“gut microbiota”.At a rough estimate,the human gut microbiome contains almost 3.3 million genes,which are about 150 times more than the total human genes present in the human *** vast amount of genetic information produces various enzymes and physiologically active ***,the gut microbiota contributes to the maintenance of host health;however,when healthy microbial composition is perturbed,a condition termed“dysbiosis”,the altered gut microbiota can trigger the development of various gastrointestinal *** gut microbiota has consequently become an extremely important research area in *** is also expected that the results of research into the gut microbiota will be applied to the prevention and treatment of human gastrointestinal diseases.A randomized controlled trial conducted by a Dutch research group in 2013 showed the positive effect of fecal microbiota transplantation(FMT)on recurrent Clostridioides difficile infection(CDI).These findings have led to the development of treatments targeting the gut microbiota,such as probiotics and FMT for inflammatory bowel diseases(IBD)and other *** review focuses on the association of the gut microbiota with human gastrointestinal diseases,including CDI,IBD,and irritable bowel *** also summarize the therapeutic options for targeting the altered gut microbiota,such as probiotics and FMT.

关键词： Inflammatory bowel disease Clostridioides difficile(Clostridium)infection Irritable bowel syndrome Probiotics Fecal microbiota transplantation

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Large edge magnetism in oxidized few-layer black phosphorus nanomeshes

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Nano Research 2017年第2期10卷 718-728页

作者： Yudai Nakanishi Ayumi Ishi Chika Ohata David Soriano Ryo Iwaki kyoko Nomura~ Miki Hasegawa Taketomo Nakamura Shingo Katsumoto Stephan Roche Junji Haruyama Faculty of Science and Engineering Aoyama Gakuin University 5- I0-1 Fuchinobe Sagamihara Kanagawa 252-5258 Japan Chemistry and Biological Science Aoyama Gakuin University 5-10-1 Fuchinobe Sagamihara Kanagawa 252-5258 Japan Catalan Institute of Nanoscience and Nanotechnology (ICN2) CSIC and The Barcelona Institute of Science and Technology Campus UAB Bellaterra 08193 Barcelona Spain Institucio Catalana de Recercai Estudis Avancats (ICREA) 08010 Barcelona Spain Institute for solid state physics The University of Tokyo 5- I-5 Kashiwanoha Kashiwa Chiba 277-8581 Japan

The formation and control of a room-temperature magnetic order in two- dimensional （2D） materials is a challenging quest for the advent of innovative magnetic- and spintronic-based technologies. To date, edge magnetism in 2D materials has been experimentally observed in hydrogen （H）-terminated graphene nanoribbons （GNRs） and graphene nanomeshes （GNMs）, but the measured magnetization remains far too small to allow envisioning practical applications. Herein, we report experimental evidences of large room-temperature edge ferromagnetism （FM） obtained from oxygen （O）-terminated zigzag pore edges of few-layer black phosphorus （P） nanomeshes （BPNMs）. The magnetization values per unit area are -100 times larger than those reported for H-terminated GNMs, while the magnetism is absent for H-terminated BPNMs. The magnetization measurements and the first-principles simulations suggest that the origin of such a magnetic order could stem from ferromagnetic spin coupling between edge P with O atoms, resulting in a strong spin localization at the edge valence band, and from uniform oxidation of full pore edges over a large area and interlayer spin interaction. Our findings pave the way for realizing high-efficiency 2D flexible magnetic and spintronic devices without the use of rare magnetic elements.

关键词： black phosphorus nanomesh edge magnetism spintronics zigzag edge oxygen termination rare-metal free

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Radiofrequency ablation as treatment for pulmonary metastasis of colorectal cancer

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World Journal of Gastroenterology 2014年第4期20卷 988-996页

作者： Takao Hiraki Hideo Gobara Toshihiro Iguchi Hiroyasu Fujiwara Yusuke matsui Susumu Kanazawa Department of Radiology Okayama University Medical School

Radiofrequency ablation (RFA) causes focal coagulation necrosis in tissue. Its first clinical application was reported in 2000, and RFA has since been commonly used in both primary and metastatic lung cancer. The procedure is typically performed using computed tomography guidance, and the techniques for introducing the electrode to the tumor are simple and resemble those used in percutaneous lung biopsy. The most common complication is pneumothorax, which occurs in up to 50% of procedures; chest tube placement for pneumothorax is required in up to 25% of procedures. Other severe complications, such as pleural effusion requiring chest tube placement, infection, and nerve injury, are rare. The local efficacy depends on tumor size, and local progression after RFA is not rare, occurring in 10% or more of patients. The local progression rate is particularly high for tumors > 3 cm. Repeat RFA may be used to treat local progression. Short- to mid-term survival after RFA appears promising and is approximately 85%-95% at 1 year and 45%-55% at 3 years. Long-term survival data are sparse. Better survival may be expected for patients with small metastasis, low carcinoembryonic antigen levels, and/or no extrapulmonary metastasis. The notable advantages of RFA are that it is simple and minimally invasive; preserves pulmonary function; can be repeated; and is applicable regardless of previous treatments. Its most substantial limitation is limited local efficacy. Although surgery is still the method of choice for treatment with curative intent, the ultimate application of RFA may be to replace metastasectomy for small metastases. Randomized trials comparing RFA with surgery are needed.

关键词： Radiofrequency ablation Pulmonary metastases Colorectal cancer Ablation therapy Lung

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Predictors of Mothers’ Early Postpartum Fatigue: A Cross-Sectional Study

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Open Journal of Nursing 2020年第9期10卷 890-902页

作者： Natsuko Kojima kyoko Asazawa Division of Nursing Faculty of Nursing Tokyo Healthcare University Tokyo Japan

Background: Most postpartum mothers experience fatigue. However, it was unclear which day after birth during hospitalization mothers are the most fatigued, and if the mothers’ background is a factor. The aim of this study was to investigate hospitalized mothers’ characteristic features, fatigue, and relaxation, and then to find the correlated factors of fatigue in order to obtain suggestions for mothers’ early postpartum care. Methods: This quantitative cross-sectional descriptive correlational study occurred during May to December 2016. A purposive sample of early postpartum hospitalized mothers at a postpartum unit in a Tokyo hospital completed an anonymous self-filled questionnaire survey. The survey included respondents’ characteristics, fatigue scale, and relaxation scale. Data were analyzed with descriptive statistics, independent t-test, one-way analysis of variance, multiple regression analyses, and structural equation modeling. Results: Returned questionnaires were 251 (83.4%). Fatigue was significantly higher for mothers with longer postpartum days compared to shorter postpartum days (F = 5.7, p β = −0.30, p β = 0.26, p β = 0.14, p Conclusions: No relaxation, longer postpartum days, and previous disease were associated with Fatigue for early postpartum mothers. These results suggest that focusing on previous disease, length of postpartum days, and mother’s relaxation is needed to provide full support to early postpartum mothers.

关键词： Postpartum Mothers Fatigue Relaxation

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Improvement of photocatalytic activity of high specific surface area graphitic carbon nitride by loading a co-catalyst

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Rare Metals 2019年第5期38卷 468-474页

作者： Ye Chen Naoya Murakami Hai-Yan Chen Jia Sun Qi-Tao Zhang Zhi-Feng Wang Teruhisa ohno Ming Zhang Department of Materials Science Faculty of EngineeringKyushu Institute of TechnologyKitakyushu 8048550Japan School of Chemistry and Chemical Engineering Yangzhou UniversityYangzhou 225002China Yangzhou Polytechnic Institute Yangzhou 225127.China Japan Science and Technology Agency Kawaguchi 3320012Japan

Graphitic carbon nitride(g-C3N4)was synthesized by heating melamine and was then treated with sodium hydroxide solution under a hydrothermal condition to obtain g-C3N4 with a large specific surface area(HSSA).HSSA shows higher photocatalytic activity for decomposition of acetaldehyde than that of original *** was modified with RuO2 as a co-catalyst by the impregnation *** loaded with 0.05 wt%RuO2 shows the highest photocatalytic activity for acetaldehyde decomposition under visible light(k=455 nm).

关键词： Graphitic carbon nitride Organic decomposition Co-catalyst Visible light

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New fluorimetric assay of horseradish peroxidase using sesamol as substrate and its application to EIA

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Journal of Pharmaceutical Analysis 2012年第2期2卷 156-159页

作者： Hidetoshi Arakawa Shigeo Nakabayashi Ken-ichi ohno Masako Maeda School of Pharmacy Showa University

Horseradish peroxidase (HRP) is generally used as a label enzyme in enzyme immunoassay (EIA).The procedure used for HRP detection in EIA is critical for sensitivity and *** paper describes a novel fluorimetric assay for horseradish peroxidase (HRP) using sesamol as *** principle of the assay is as follow:sesamol (3,4-methylenedioxy phenol) is reacted enzymatically in the presence of hydrogen peroxide to produce dimeric *** dimer is fluorescent and can be detected sensitively at ex.347 nm,em.427 *** measurable range of HRP was 1.0×10-18 to 1.0×10-15 mol/assay,with a detection limit of 1.0×10-18 mol/*** coefficient of variation (CV,n=8) was examined at each point on the standard curve,with a mean CV percentage of 3.8%.This assay system was applied to thyroid stimulating hormone (TSH) EIA using HRP as the label enzyme.

关键词： Sesamol Fluorescence Enzyme immunoassay (EIA) Horseradish peroxidase (HRP) Thyroid stimulating hormone (TSH)

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Metabolomic Screening Applied to Rice FOX Arabidopsis Lines Leads to the Identification of a Gene-Changing Nitrogen Metabolism

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Molecular Plant 2010年第1期3卷 125-142页

作者： Doris Albinsky Miyako Kusano Mieko Higuchi Naomi Hayashi Makoto Kobayashi Atsushi Fukushima Masaki Mori Takanari Ichikawa Keiko matsui Hirofumi Kuroda Yoko Horii Yuko Tsumoto Hitoshi Sakakibara Hirohiko Hirochika Minami matsui Kazuki Saito RIKEN Plant Science Center Tsurumi-ku Yokohama 230-0045 Japan National Institute of Agrobiological Sciences Tsukuba 305-8602 Japan Graduate School of Pharmaceutical Sciences Chiba University Inage-ku Chiba 263-8522 Japan

Plant metabolomics developed as a powerful tool to examine gene functions and to gain deeper insight into the physiology of the plant cell. In this study, we screened Arabidopsis lines overexpressing rice full-length （FL） cDNAs （rice FOX Arabidopsis lines） using a gas chromatography-time-of-flight mass spectrometry （GC-TOF/MS）-based technique to identify rice genes that caused metabolic changes. This screening system allows fast and reliable identification of candi- date lines showing altered metabolite profiles. We performed metabolomic and transcriptomic analysis of a rice FOX Ara- bidopsis line that harbored the FL cDNA of the rice ortholog of the Lateral Organ Boundaries （LOB） Domain （LBD）/ Asymmetric Leaves2-1ike （ASL） gene of Arabidopsis, At-LBD37/ASL39. The investigated rice FOX Arabidopsis line showed prominent changes in the levels of metabolites related to nitrogen metabolism. The transcriptomic data as well as the results from the metabolite analysis of the Arabidopsis At-LBD37/ASL39-overexpressor plants were consistent with these findings. Furthermore, the metabolomic and transcriptomic analysis of the Os-LBD37/ASL39-overexpressing rice plants indicated that Os-LBD37/ASL39 is associated with processes related to nitrogen metabolism in rice. Thus, the combination of a metabolomics-based screening method and a gain-of-function approach is useful for rapid characterization of novel genes in both Arabidopsis and rice.

关键词： Metabolite profiling GC-TOF/MS rice FOX Arabidopsis lines transcript profiling LBD37/ASL39 nitrogenmetabolism.

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