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Electrically controlled mRNA delivery using a polypyrrolegraphene oxide hybrid film to promote osteogenic differentiation of human mesenchymal stem cells

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Nano Research 2022年第10期15卷 9253-9263页

作者： Huijung Kim Kübra Solak Yoojoong Han Yeon-Woo Cho kyeong-mo koo Chang-Dae Kim Zhengtang Luo Hyungbin Son Hyung-Ryong Kim Ahmet Mavi Tae-Hyung Kim School of Integrative Engineering Chung-Ang University84 Heukseuk-roDongjak-guSeoul 06974Republic of Korea Department of Nanoscience and Nanoengineering Graduate School of Natural and Applied SciencesAtatürk UniversityErzurum 25240Turkey Department of Chemical and Biological Engineering Hong Kong University of Science and TechnologyKowloonHong Kong 999077China Department of Pharmacology College of DentistryJeonbuk National UniversityJeonju 54896Republic of Korea Department of Nanoscience and Nanoengineering Institute of ScienceAtatürk UniversityErzurum 25240Turkey Department of Mathematics and Science Education Education Faculty of Kazım KarabekirAtatürk UniversityErzurum 25240Turkey

Direct messenger ribonucleic acid(mRNA)delivery to target cells or tissues has revolutionized the field of ***,the applicability of regenerative medicine is limited by the technical difficulties of various mRNA-loaded ***,we report a new conductive hybrid film that could guide osteogenic differentiation of human adipose-derived mesenchymal stem cells(hADMSCs)via electrically controlled mRNA *** find optimal electrical conductivity and mRNAloading capacity,the polypyrrole-graphene oxide(PPy-GO)hybrid film was electropolymerized on indium tin oxide *** found that the fluorescein sodium salt,a molecule partially mimicking the physical and chemical properties of mRNAs,can be effectively absorbed and released by electrical stimulation(ES).The hADMSCs cultivated on the PPy-GO hybrid film loaded with pre-osteogenic mRNAs showed the highest osteogenic differentiation under electrical *** platform can load various types of RNAs thus highly promising as a new nucleic acid delivery tool for the development of stem cell-based therapeutics.

关键词： messenger ribonucleic acid(mRNA)delivery polypyrrole graphene oxide electrical release mesenchymal stem cells

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Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein(RNP)electroporation

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Journal of Animal Science and Biotechnology 2024年第1期15卷 456-462页

作者： Gyeong-Min Gim kyeong-Hyeon Eom Dong-Hyeok Kwon Dae-Jin Jung Dae-Hyun Kim Jun-koo Yi Jae-Jung Ha Ji-Hyun Lee Seong-Beom Lee Woo-Jae Son Soo-Young Yum Won-Wu Lee Goo Jang Laboratory of Theriogenology and Biotechnology Department of Veterinary Clinical ScienceCollege of Veterinary Medicine and the Research Institute of Veterinary ScienceSeoul National UniversitySeoulRepublic of Korea LARTBio Co. Ltd.SeoulRepublic of Korea Gyeongsangbukdo Livestock Research Institute YeongjuRepublic of Korea Comparative Medicine Disease Research Center Seoul National UniversitySeoulRepublic of Korea

Background Genome editing has been considered as powerful tool in agricultural ***,genome editing progress in cattle has not been fast as in other mammal species,for some disadvantages including long gestational periods,single pregnancy,and high raising ***,technically demanding methods such as microinjection and somatic cell nuclear transfer(SCNT)are needed for gene editing in *** this point of view,electroporation in embryos has been risen as an *** First,editing efficiency of our electroporation methods were tested for *** of mutation on embryo was confirmed by T7E1 *** first combination,mutation rates for MSTN and PRNP were 57.6%±13.7%and 54.6%±13.5%,*** case of MSTN/BLG,mutation rates were 83.9%±23.6%for MSTN,84.5%±18.0%for ***,the double-KO embryos were transferred to surrogates and mutation rate was identified in resultant calves by targeted deep *** recipients were transferred for MSTN/PRNP,4 calves were delivered,and one calf underwent an induction for double *** surrogates were given double-KO embryos for MSTN/BLG,and four of the six calves that were born had mutations in both *** These data demonstrated that production of genome edited cattle via electroporation of RNP could be effectively ***,MSTN and PRNP from beef cattle and MSTN and BLG from dairy cattle have been born and they will be valuable resources for future precision breeding.

关键词： Beta-lactoglobulin Cattle CRISPR-Cas9 Electroporation Knockout MSTN PRNP

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Fabrication of sintering-free flexible copper nanowire/ polymer composite transparent electrodes with enhanced chemical and mechanical stability

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Nano Research 2016年第7期9卷 2162-2173页

作者： Cho Rong Chu Changsoo Lee Jahyun koo Hyuck mo Lee Department of Materials Science and Engineering KAIST 291 Daehak-ro Yuseong-gu Daejeon 305-701 Republic of Korea

The thermal decomposition synthesis of long copper nanowires （CuNWs） was achieved by controlling the synthesis parameters. A detailed study was performed to determine the effect of the molar ratio of copper chloride to nickel acetylacetonate, temperature, and stirring rate on the final shape of the products. Transparent electrodes （TEs） were fabricated by wet treatment with acetic acid （AA）, without using a sintering process. The low oxidation stability and high surface roughness are the main disadvantages of the CuNW TEs, which limit their applications. In order to overcome these issues, we prepared CuNW/polymer composite TEs by partial embedding of the CuNWs into poly（methyl methacrylate）（PMMA） on poly（ethylene terephthalate）（PET） substrates. The CuNW/PMMA composite TEs exhibit excellent optoelectronic performance （91.3% at 100.7 ff2/sq）, low surface roughness （4.6 nm in height）, and good mechanical and chemical stability as compared with CuNW TEs. On the basis of these properties, we believe that CuNW-based composite TEs could serve as low-cost materials for a wide range of new optoelectronic devices.

关键词： copper nanowires nanowire/polymercomposite transparent electrode chemical stability mechanical stability

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Enhanced refolding of lysozyme with imidazolium-based room temperature ionic liquids: Effect of hydrophobicity and sulfur residue

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Science China Chemistry 2012年第8期55卷 1657-1662页

作者： BAE Sang-Woo CHANG Woo-Jin koo Yoon-mo HA Sung Ho Department of Biological Engineering Inha University Incheon 402-751 Republic of Korea Department of Mechanical Engineering School of Freshwater Sciences University of Wisconsin-Milwaukee Milwaukee WI 53201-0413 USA Department of Chemical Engineering and Nano-Bio Technology Hannam University Daejeon 305-811 Republic of Korea

The expression of recombinant proteins in microorganism frequently leads to the formation of insoluble aggregates, inclusion bodies （IBs）. Thus, the additional in vitro protein refolding process is required to convert inactive IBs into water-soluble active proteins. This study investigated the effect of sulfur residue and hydrophobicity of imidazolium-based room temperature ionic liquids （RTILs） on the refolding of lysozyme as a model protein in the batch dilution method which is the most commonly used refolding method. When lysozyme was refolded in the refolding buffer containing [BF4]-based RTILs with a systematic variety of alkyl chain on cations varying from two to eight, less hydrophobic imidazolium cations having shorter alkyl chains were effective to facilitate lysozyme refolding. Compared to the conventional refolding buffer, 2 times higher lysozyme re- folding yield was obtained in l-ethyl-3-methylimidazolium tetrafluoroborate （[EMIM][BF4]） containing refolding buffer. The refolding yield of lysozyme was even more increased by 2.5 times when 1-butyl-3-methylimidazolium methylsulfate （[BMIM][MS]） containing sulfur residue on anion was used. The sulfur residue in [BMIM][MS] is supposed to improve the refolding yield of lysozyme which has 4 intrarnolecular disulfide bonds. For dilution-based refolding of lysozyme, the opti- mum concentrations of RTILs in refolding buffer were found to be 1.0 M [EMIM][BF4] and 0.5 M [PMIM][MS], respectively. The optimum temperate for dilution-based refolding of lysozyme with RTILs was 4 ℃.

关键词： protein refolding room temperature ionic liquid dilution refolding lysozyme hydrophobicity sulfur

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Bayesian meta-analysis of regional biomass factors for Quercus mongolica forests in South Korea

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Journal of Forestry Research 2015年第4期26卷 875-885页

作者： Tzeng Yih Lam Xiaodong Li Rae Hyun Kim kyeong Hak Lee Yeong mo Son Division of Forest Management Department of Forest and Climate Change Korea Forest Research Institute57 Hoegiro Dongdaemun-gu Seoul 130-712 South Korea School of Forestry and Resource Conservation National Taiwan University

Indirect approaches to estimation of biomass factors are often applied to measure carbon flux in the forestry sector. An assumption underlying a country-level carbon stock estimate is the representativeness of these factors. Although intensive studies have been conducted to quantify biomass factors, each study typically covers a limited geographic area. The goal of this study was to employ a meta-analysis approach to develop regional bio- mass factors for Quercus mongolica forests in South Korea. The biomass factors of interest were biomass conversion and expansion factor （BCEF）, biomass expansion factor （BEF） and root-to-shoot ratio （RSR）. Our objectives were to select probability density functions （PDFs） that best fitted the three biomass factors and to quantify their means and uncertainties. A total of 12 scientific publications were selected as data sources based on a set of criteria. Fromthese publications we chose 52 study sites spread out across South Korea. The statistical model for the meta- analysis was a multilevel model with publication （data source） as the nesting factor specified under the Bayesian framework. Gamma, Log-normal and Weibull PDFs were evaluated. The Log-normal PDF yielded the best quanti- tative and qualitative fit for the three biomass factors. However, a poor fit of the PDF to the long right tail of observed BEF and RSR distributions was apparent. The median posterior estimates for means and 95 % credible intervals for BCEF, BEF and RSR across all 12 publica- tions were 1.016 （0.800-1.299）, 1.414 （1.304-1.560） and 0.260 （0.200-0.335）, respectively. The Log-normal PDF proved useful for estimating carbon stock of Q. mongolica forests on a regional scale and for uncertainty analysis based on monte Carlo simulation.

关键词： Uncertainty analysis monte Carlosimulation Bayesian hierarchical model Nestingstructure Biomass estimation

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Recent Progress in Exploiting Small molecule Peptides as Supramolecular Hydrogelators

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Chinese Journal of Polymer Science 2017年第10期35卷 1194-1211页

作者： Hao Su Yuzhu Wang Caleb F.Anderson Jin mo koo Han Wang Honggang Cui Department of Chemical and Biomolecular Engineering The Johns Hopkins University Baltimore Maryland 21218 USA Institute for NanoBio Technology The Johns Hopkins University Baltimore Maryland 21218 USA Department of Oncology and Sidney Kimmel Comprehensive Cancer Center Johns Hopkins University School of Medicine Baltimore MD 21205 USA Center for Nanomedicine The Wilmer Eye Institute Johns Hopkins University School of Medicine 400 North Broadway Baltimore MD 21231 USA

Small molecule peptides and their derivatives are an emerging class of supramolecular hydrogelators that have attracted rapidly growing interest in the fields of drug delivery and regenerative medicine due to their inherent biodegradability and biocompatibility, as well as versatility in molecular design and ease of synthesis. Built upon the directional, intermolecular interactions such as hydrogen bonding and π-π stacking, peptide-based molecular units can associate in aqueous solution into filamentous assemblies of various sizes and shapes. Under appropriate conditions, these filamentous assemblies can percolate into a 3D network with materials properties tailorable for specific biomedical applications. In this review, we survey the literature published over the past three years in the development of peptide-based hydrogelators for biomedical applications. We highlight several representative examples and center our discussion on the fundamentals of molecular design, assembly, and gelation conditions.

关键词： PNIPAM spheres reversible assembled colloidal Highly ordered assembly leaving anhydride

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A new species of Zanclognatha Lederer and three new records of the families Erebidae and Noctuidae(Lepidoptera,Noctuoidea)from Korea,with notes on larval feeding habits

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Journal of Forestry Research 2021年第3期32卷 1235-1240页

作者： Kang-Won Lee Joon-mo koo Jae-Dong Kim Hui-Lin Han Kyu-Tek Park Holoce Ecosystem Conservation Research Institution HoengseongGangweon Province 225-841Republic of Korea Department of Plant Medicine Chungbuk National UniversityCheongju28644Republic of Korea Department of Applied Biology College of Agriculture and Life ScienceChungnam National UniversityDaejeon 34134Republic of Korea School of Forestry Northeast Forestry UniversityHarbin 150040China Key Laboratory of Sustainable Forest Ecosystem Management-Ministry of Education Northeast Forestry UniversityHarbin 150040People’s Republic of China Bioresource and Environmental Center Incheon National UniversityIncheon 22012Republic of Korea

A new species of Zanclognatha Lederer,1857 of the family Erebidae,*** sp.n.,one species of Family Erebidea,Asota egens(Walker,1854)and two species of Family Noctuidae:Meganephria retinea Gyulai and Ronkay,1999 and Amphipyra subrigua Bremer and Grey,1853,are reported for the first time from *** addition,larval feeding habits with their host plants for three species are newly recognized from *** of adults,larvae for Zanclognatha querciella and Meganephria retinea,and the genitalia of male or female for all species are provided.

关键词： Host plants New species New records Korean peninsula Taxonomy

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All-Optical WDM Buffer System Realized by NOLM and Feedback Loop Structure

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光学学报 2003年第S1期23卷 765-766页

作者： Seungwoo Yi kyeong-mo Yoon Yong-Gi Lee Jinseob Eom Dept. of Electronic Engineering Kangwon National Univ. Chuncheon 200-701 KoreaKT Technology Laboratory Daejeon 305-811 KoreaKT Technology Laboratory Daejeon 305-811 KoreaDept. of Electronic Engineering Kangwon National Univ. Chuncheon 200-701 Korea

We propose an all-optical WDM buffer for optical packet switching system, which consists of NOLM and feedback loop. The proposed structure provides more than 40 turn buffering and nice output of buffered data when sel... 详细信息

关键词： WDM All-Optical WDM Buffer System Realized by NOLM and Feedback Loop Structure EDFA SOA into by NOLM than

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Stereotactic body radiotherapy for isolated paraaortic lymph node recurrence from colorectal cancer

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World Journal of Gastroenterology 2009年第48期15卷 6091-6095页

作者： Mi-Sook Kim Chul koo Cho Kwang mo Yang Dong Han Lee Sun Mi moon Young Joo Shin Department of RadiationOncology and Surgery Korea Institute of Radiological & Medical Sciences

AIM： To evaluate the efficacy and complications of stereotactic body radiotherapy in localized paraaortic lymph node recurrence from colorectal cancer. METHODS： From 2003 to 2009, 7 patients with paraaortic lymph node recurrence （1-3 lesions） from colorectal cancer were treated with stereotactic body radiotherapy. Total gross tumor volumes ranged from 4 to 40 mL. The doses were escalated from 36 Gy/patient to 51 Gy/patient and were delivered in 3 fractions. RESULTS： One and 3 year overall survival rates were 100% and 71.4%, respectively, and median survival was 37 mo. Grade IV intestinal obstruction was reported in 1 of 7 patients. This patient received 48 Gy in 3 fractions with a maximum point dose to the intestine of 53 Gy and V45Gy = 3.6 mL. However, 6 patients received an intestinal maximum point dose of 〈 51 Gy and V45Gy of 〈 1 mL, and did not develop any severe complications. CONCLUSION： This pilot study suggests selected paraaortic lymph node recurrence （1-3 closed lesions） that failed to respond to chemotherapy can be potentially salvaged by stereotactic body radiotherapy.

关键词： Stereotactic body radiotherapy Paraaortic lymph node recurrence Colorectal cancer

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Elucidating Ion Transport Phenomena in Sulfide/Polymer Composite Electrolytes for Practical Solid-State Batteries

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Nano-Micro Letters 2023年第10期15卷 416-432页

作者： kyeong‑Seok Oh Ji Eun Lee Yong‑Hyeok Lee Yi‑Su Jeong Imanuel Kristanto Hong‑Seok Min Sang‑mo Kim Young Jun Hong Sang Kyu Kwak Sang‑Young Lee Department of Chemical and Biomolecular Engineering Yonsei University50 Yonsei‑roSeodaemun‑guSeoul 03722Republic of Korea Department of Chemical and Biological Engineering Korea University145 Anam‑roSeongbuk‑guSeoul 02841Republic of Korea School of Energy and Chemical Engineering Ulsan National Institute of Science and Technology(UNIST)50 UNIST‑gilEonyang‑eupUlju‑gunUlsan 44919Republic of Korea Hyundai motor Company 150Hyundaiyeonguso‑roNamyang‑eupHwaseong‑siGyeonggi‑do 18280Republic of Korea

Despite the enormous interest in inorganic/polymer composite solid-state electrolytes(CSEs)for solid-state batteries(SSBs),the underlying ion transport phenomena in CSEs have not yet been ***,we address this issue by formulating a mechanistic understanding of bi-percolating ion channels formation and ion conduction across inorganic-polymer electrolyte interfaces in CSEs.A model CSE is composed of argyrodite-type Li_6PS_5Cl(LPSCl)and gel polymer electrolyte(GPE,including Li~+-glyme complex as an ion-conducting medium).The percolation threshold of the LPSCl phase in the CSE strongly depends on the elasticity of the GPE ***,manipulating the solvation/desolvation behavior of the Li~+-glyme complex in the GPE facilitates ion conduction across the LPSCl-GPE *** resulting scalable CSE(area=8×6(cm×cm),thickness~40μm)can be assembled with a high-mass-loading LiNi_(0.7)Co_(0.15)Mn_(0.15)O_(2)cathode(areal-mass-loading=39 mg cm~(-2))and a graphite anode(negative(N)/positive(P)capacity ratio=1.1)in order to fabricate an SSB full cell with bi-cell *** this constrained cell condition,the SSB full cell exhibits high volumetric energy density(480 Wh L_(cell)~(-1))and stable cyclability at 25℃,far exceeding the values reported by previous CSE-based SSBs.

关键词： Solid-state batteries Composite solid-state electrolytes Ion transport phenomena Bi-percolating ion channels Interfacial resistance

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