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Enrichment-ELIsA for Detection of salmonella typhi From Food and Water samples

Biomedical and Environmental sciences 2008年第2期21卷 137-143页

作者： s. kumar K. BALAKRIsHNA HV. BATRA Division of Microbiology Defence R & D Establishment Jhansi Road Gwalior 474002 India

Objective Development of monoclonal antibody based sandwich enzyme linked immunosorbant assay （sELIsA） for rapid detection of salmonella enterica serovar typhi （s. typhi） from food and water samples and optimization of enrichment procedures for use with the developed sELIsA to increase the detection sensitivity of the assay. Methods spleen cells from BALB/c mice immunized with flagellin （H=d） antigen of s. typhi were fused with sp2/0 myeloma cells. The hybridoma cell line specific to H=d antigen was established, characterized and ascites raised against one of these clones. The hyperimmune serum to flagellin antigen was raised in New Zealand White rabbits. An sELIsA was developed using polyclonal antibody as capture and monoclonal antibody as detection antibody. To design the efficient culture strategies for use with the sELIsA, different pre-enrichment and enrichment broths were evaluated. The media included buffered peptone water （BPW） and brain heart infusion broth for pre-enrichment and selenite F broth and Rappaport-Vassiliadis broth as enrichment broths. The developed sELIsA with preceding enrichment step in BPW （Enrichment-ELIsA） was evaluated in various food samples artificially inoculated with s. typhi bacteria. Various food （30） and water （35） samples collected from field were also tested by Enrichment-ELIsA and culture method. Results Out of four specific clones to H=d antigen, one clone （# 2/56, IgG2a isotype） was used in sELIsA. The sELIsA had the detection limit of 10^4-10^5 cfu of s. typhi. Of the various broths used with sELIsA, BPW was found to yield maximum ELIsA values. Enrichment-ELIsA, when tested in artificially inoculated food samples, generally, could detect 10^2 s. typhi cfu/mL within 10 h from various food rinses （meat, vegetable） and milk samples. After overnight enrichment in BPW, as less as 2 bacteria per 10 mL of milk, meat rinse, and chicken rinse could be detected. Only one of the field samples （water） gave false positive result by Enr

关键词： Enrichment-ELIsA Food Monoclonal antibody salmonella sandwich ELIsA Water

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Numerical and analytical investigations of thermosolutal instability in rotating Rivlin-Ericksen fluid in porous medium with Hall current

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Applied Mathematics and Mechanics(English Edition) 2013年第4期34卷 501-522页

作者： s.kumar V.sHARMA K.KIsHOR Department of Mathematics Government College Department of Mathematics Himachal Pradesh University Department of Mathematics Government Senior Secondary School

Numerical and analytical investigations of the thermosolutal instability in a viscoelastic Rivlin-Ericksen fluid are carried out in the presence of a uniform vertical magnetic field to include the Hall current with a uniform angular velocity in a porous medium. For stationary convection, the stable solute gradient parameter and the rota- tion have stabilizing effects on the system, whereas the magnetic field and the medium permeability have stabilizing or destabilizing effects on the system under certain condi- tions. The Hall current in the presence of rotation has stabilizing effects for sufficiently large Taylor numbers, whereas in the absence of rotation, the Hall current always has destabilizing effects. These effects have also been shown graphically. The viscoelastic effects disappear for stationary convection. The stable solute parameter, the rotation, the medium permeability, the magnetic field parameter, the Hall current, and the vis- coelasticity introduce oscillatory modes into the system, which are non-existent in their absence. The sufficient conditions for the non-existence of overstability are also obtained.

关键词： magnetic field thermosolutal instability Rivlin-Ericksen rotating fluid Hall current porous medium

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Detection of Chlorophenolic Compounds in Bleaching Effluents of Chemical Pulps

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中国造纸 2008年第5期27卷 35-40页

作者： Chhaya sharma s. Mohanty s. kumar N.J. Rao Department of Paper Technology IIT-Roorkee India Gas Authority of India Ltd. Calcutta India JP Institute Gunna India

Laboratory bleaching effluents from the chlorination and caustic extraction stages of mixed wood kraft pulp processing have been analysed both qualitatively and quantitatively for various chlorophenolics by using GC.A number of chlorinated derivaties of phenols,catechols,guaiacols and syringaldehydes have been detected and their concentrations are *** results are compared with that of different agriculture residue/hardwood pulps,which were reported in *** concentrations of various compounds detected have also been compared with their reported 96LC50 values.

关键词：化学纸浆漂白方法制造方法造纸技术

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Pharmacognostic study and anti-inflammatory activity of Callistemon lanceolatus leaf

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Asian Pacific Journal of Tropical Biomedicine 2011年第3期1卷 177-181页

作者： kumar s kumar V Prakash OM Institute of Pharmaceutical sciences Kurukshetra University

Objective:To study detail pharmacognosy and anti-inflammatory activity of Callistemon lanceolatus(***) ***:Leaf sample was studied by organoleptic, macroscopical,microscopical,phytochemical and other WHO recommended methods for *** methanolic leaf extract of the plant was also screened for antiinflammatory activity on carrageenan-induced paw edema in rat at doses of 200 and 400 mg/kg, *** detail pharmacognostic study of the *** leaf was carried out to lay down the standards which could be useful in future experimental ***:*** methanolic leaf extract showed significant(Ps) anti-inflammatory activity at doses of 200 mg/kg and 400 mg/*** significant anti-inflammatory of *** methanolic leaf extract at the dose of 400 mg/kg was comparable with diclofenac ***:The pharmacognostic profile of the *** leaf is helpful in standardization for quality,purity and sample *** methanolic extract at a dose of 400 mg/kg shows a significant activity in comparison with the standard drug diclofenac sodium(50 mg/kg).

关键词： Anti-inflammatory Callistemon lanceolatus Carragennan Fibre Microscopy stomata

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Microscopic evaluation and physiochemical analysis of Dillenia indica leaf

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Asian Pacific Journal of Tropical Biomedicine 2011年第5期1卷 337-340页

作者： s kumar V kumar Om Prakash Institute of Pharmaceutical sciences Kurukshetra University

Objective:To study detail microscopic evaluation and physiochemical analysis of Dillenia indica(***)***:Fresh leaf sample and dried power of the leaf were studied macroscopically and *** phytochemical investigation of plant material was *** WHO recommended parameters for standardizations were also ***:The detail microscopy revealed the presence of anomocytic stomata,unicellular trichome,xylem fibres,calcium oxalate crystals,vascular bundles,*** constants such as stomatal number,stomatal index,vein-islet number and veinlet termination numbers were also *** parameters such as ash values,loss on drying,extractive values,percentage of foreign matters,swelling index,*** also *** phytochemical screening showed the presence of steroids,terpenoids,glycosides,fatty acids,flavonoids,phenolic compounds and ***:The microscopic and physiochemical analysis of the *** leaf is useful in standardization for quality,purity and sample identification.

关键词： Dillenia indica Fibre Leaf constant Microscopy Physiochemical stomata Xylem

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Antidiabetic and hypolipidemic activities of Kigelia pinnata flowers extract in streptozotocin induced diabetic rats

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Asian Pacific Journal of Tropical Biomedicine 2012年第7期2卷 543-546页

作者： kumar s kumar V Prakash OM Institute of Pharmaceutical sciences Kurukshetra University

Objective:To evaluate antidiabetic and hypolipidemic activities of Kigelia pinnata rnethanolic(lowers extract in slreplozolocin(sTZ) induced diabetic W islar *** were made diabetic by a single dose of sTZ at 60 mg/kg body weight *** blood glucose level was checked before and 72 h after sTZ injection to confirm the development ot *** flower extract and glibenclamide were administered orally at the doses of 250 and 500 mg/kg body weight for 21 ***:Daily oral treatment with the extract and standard drug for 21 days significantly reduced blood glucose,serum cholesterol and triglycerides *** density lipoprotein-cholesterol level was found to be improved(Ps compared to diabetic control ***:It is concluded that Kigellia pinnata flowers extract have significant antidiabetic and hypolipidemic effect.

关键词： Antidiabetic Kigelia pinnata streptozotocin

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Evolutionary Algorithm Based Feature subset selection for students Academic Performance Analysis

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Intelligent Automation & soft Computing 2023年第6期36卷 3621-3636页

作者： Ierin Babu R.Mathusoothana s.kumar Department of Computer science and Engineering Noorul Islam Centre for Higher EducationKanyakumari629180India Department of Information Technology Noorul Islam Centre for Higher EducationKanyakumari629180India

Educational Data Mining(EDM)is an emergent discipline that concen-trates on the design of self-learning and adaptive *** education institutions have started to utilize analytical tools to improve students’grades and *** of students’performance is a difficult process owing to the massive quantity of educational ***,Artificial Intelligence(AI)techniques can be used for educational data mining in a big data *** the same time,in EDM,the feature selection process becomes necessary in creation of feature *** the feature selection performance affects the predictive performance of any model,it is important to elaborately investigate the outcome of students’performance model related to the feature selection *** this motivation,this paper presents a new Metaheuristic Optimiza-tion-based Feature subset selection with an Optimal Deep Learning model(MOFss-ODL)for predicting students’*** addition,the proposed model uses an isolation forest-based outlier detection approach to eliminate the existence of ***,the Chaotic Monarch Butterfly Optimization Algo-rithm(CBOA)is used for the selection of highly related features with low com-plexity and high ***,a sailfish optimizer with stacked sparse autoencoder(sFO-ssAE)approach is utilized for the classification of educational *** MOFss-ODL model is tested against a benchmark student’s perfor-mance data set from the UCI repository.A wide-ranging simulation analysis por-trayed the improved predictive performance of the MOFss-ODL technique over recent approaches in terms of different *** to other methods,experimental results prove that the proposed(MOFss-ODL)classification model does a great job of predicting students’academic progress,with an accuracy of 96.49%.

关键词： students’performance analysis educational data mining feature selection deep learning metaheuristics outlier detection

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Low temperature Raman study of PrCoO_3 thin films on LaAlO_3(100) substrates grown by pulsed laser deposition

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Journal of Central south University 2010年第6期17卷 1144-1147页

作者： PRAKAsH R kumar s LEE C G sONG JI Department of Mechanical Engineering Changwon National UniversityChangwon 641773Korea school of Nano and Advanced Materials Engineering Changwon National UniversityChangwon 641773Korea

Thin films of PrCoO3 were deposited on LaAlO3 substrates by pulsed laser deposition technique.X-ray diffraction result indicates that films are single phase and c-axis *** investigate the spin state transition,Raman spectroscopy measurements were performed at different *** position of the Raman modes is found to increase while full width at half maximum(FWHM) of these modes is found to decrease with the decrease of temperature across spin state transition temperature(220 K) of PrCoO3.

关键词： PrCoO3 thin films Raman spectroscopy pulsed laser deposition

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Inhibition of protein kinases by anticancer DNA intercalator, 4-butylaminopyrimido[4',5':4,5]thieno(2,3-b)quinoline

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Acta Pharmaceutica sinica B 2017年第3期7卷 303-310页

作者： HeggoduG.Rohitkumar Chethan s.kumar Hulihalli N.Kiran kumar Gopal M.Advi Rao Department of Biochemistry Davangere UniversityShivagangotriTholahunaseDavangere 577002India ss Institute of Medical sciences and Research Centre JnanashankaraDavangere 577005India

Targeting protein kinases(PKs) has been a promising strategy in treating cancer, as PKs are key regulators of cell survival and proliferation. Here in this study, we studied the ability of pyrimido[4',5':4,5]thieno(2,3-b)quinolines(PTQ) to inhibit different PKs by performing computational docking and in vitro screening. Docking studies revealed that 4-butylaminopyrimido[4',5':4,5]thieno(2,3-b)quinoline(BPTQ) has a higher order of interaction with the kinase receptors than other PTQ *** vitro screening confirms that BPTQ inhibits VEGFR1 and CHK2, with the IC_(50) values of 0.54 and1.70 mmol/L, respectively. Further, cytotoxicity of BPTQ was measured by trypan blue assay. Treatment with BPTQ decreased the proliferation of HL-60 cells with an IC_(50) value of 12 mmol/L and induces apoptosis, as explicated by the fall in the mitochondrial membrane potential, annexin V labeling and increased expression of caspase-3. Taken together, these data suggest that BPTQ possess ability to inhibit PKs and to induce cell death in human promyelocytic leukemia cells.

关键词： DNA intercalator Molecular docking Kinase inhibitor Anticancer drugs Apoptosis Chemotherapy

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Gas Chromatography Analysis of Resin and Fatty Acids from Laboratory Generated Bleach Plant Effluents

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中国造纸 2007年第5期26卷 29-33页

作者： Chhaya sharma s. Mohanty s. kumar N.J. Rao Department of Paper Technology llT-Roorkeelndia Gas Authority of India Ltd. Calcutta India JP Institute Gunna India

Laboratory generated spent bleached liquor from the chlorination, caustic extraction stage of mixed wood kraft pulp processing has been analyzed both qualitatively and quantitatively for various resin & fatty acids by using GC. A number of resin acids, saturated and unsaturated fatty acids, chloro fatty and resin acid have been detected and their concentrations are estimated. The results are compared with results on different agriculture residue/hardwood pulps, which were reported earlier. The concentrations of various compounds detected have also been compared with their reported LC50 values.

关键词：气相色谱分析脂肪酸树脂酸漂白废液

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