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Plant RNA-binding proteins:Phase separation dynamics and functional mechanisms underlying plant development and stress responses

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Molecular Plant 2024年第4期17卷 531-551页

作者： sheng Fan Yu zhang Shaobo Zhu Lisha Shen Temasek Life Sciences Laboratory 1 Research LinkNational University of Singapore1 Research LinkSingapore 117604Singapore Department of Biological Sciences National University of Singapore14 Science Drive 4Singapore 117543Singapore

RNA-binding proteins(RBPs)accompany RNA from synthesis to decay,mediating every aspect of RNA metabolism and impacting diverse cellular and developmental processes in *** RBPs undergo phase separation along with their bound RNA to form and function in dynamic membraneless biomolecular condensates for spatiotemporal coordination or regulation of RNA *** evidence suggests that phase-separating RBPs with RNA-binding domains and intrinsically disordered regions play important roles in plant development and stress ***,we summarize the current knowledge about how dynamic partitioning of RBPs into condensates controls plant development and enables sensing of experimental changes to confer growth plasticity under stress conditions,with a focus on the dynamics and functional mechanisms of RBP-rich nuclear condensates and cytoplasmic granules in mediating RNA *** also discuss roles of multiple factors,such as environmental signals,protein modifications,and N6-methyladenosine RNA methylation,in modulating the phase separation behaviors of RBPs,and highlight the prospects and challenges for future research on phase-separating RBPs in crops.

关键词： RNA-binding proteins phase separation biomolecular condensates RNA metabolism development stress resilience

A CSMA/CA based MAC protocol for hybrid Power-line/Visible-light communication networks:Design and analysis

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Digital Communications and Networks 2024年第2期10卷 481-497页

作者： sheng Hao Huyin zhang Fei Yang Chenghao Li Jing Wang School of Computer Science Central China Normal UniversityWuhan430079PR China Hubei Key Laboratory of intelligent Robot(Wuhan Institute of Technology) Wuhan430079PR China School of Computer Science Wuhan UniversityWuhan430079China School of Information Science and Engineering Shandong Normal UniversityJinan250000China School of Computer Science Hubei University of TechnologyWuhan430079China

Hybrid Power-line/Visible-light Communication(HPVC)network has been one of the most promising Cooperative Communication(CC)technologies for constructing Smart Home due to its superior communication reliability and hardware *** research on HPVC networks focuses on the performance analysis and optimization of the Physical(PHY)layer,where the Power Line Communication(PLC)component only serves as the backbone to provide power to light Emitting Diode(LED)*** designing a Media Access Control(MAC)protocol remains a great challenge because it allows both PLC and Visible Light Communication(VLC)components to operate data transmission,i.e.,to achieve a true HPVC network *** solve this problem,we propose a new HPC network MAC protocol(HPVC MAC)based on Carrier Sense Multiple Access/Collision Avoidance(CSMA/CA)by combining IEEE 802.15.7 and IEEE 1901 ***,we add an Additional Assistance(AA)layer to provide the channel selection strategies for sensor stations,so that they can complete data transmission on the selected channel via the specified CSMA/CA mechanism,*** on this,we give a detailed working principle of the HPVC MAC,followed by the construction of a joint analytical model for mathematicalmathematical validation of the HPVC *** the modeling process,the impacts of PHY layer settings(including channel fading types and additive noise feature),CSMA/CA mechanisms of 802.15.7 and 1901,and practical configurations(such as traffic rate,transit buffer size)are comprehensively taken into ***,we prove the proposed analytical model has the ***,through extensive simulations,we characterize the HPVC MAC performance under different system parameters and verify the correctness of the corresponding analytical model with an average error rate of 4.62%between the simulation and analytical results.

关键词： Hybrid power-line/Visible light communication (HPVC)networks MAC protocol CSMA/CA IEEE 802.15.7 IEEE 1901 Performance analysis

Solvent-free carbon sphere nanofluids towards intelligent lubrication regulation

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Friction 2024年第1期12卷 95-109页

作者： sheng zhang Wen LI Xiaoliang MA Xiaoqiang FAN Minhao ZHU Tribology Research Institute School of Mechanical EngineeringSouthwest Jiaotong UniversityChengdu 610031China Key Laboratory of Advanced Technologies of Materials Ministry of EducationSchool of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China

By simply switching the electrical circuit installed on steel/steel contact,the tribological behaviors of nanofluids(NFs)can be regulated in real time,thereby achieving the desired performance of friction reduction and wear ***,solvent-free carbon spherical nanofluids(C-NFs)were successfully prepared for intelligent lubrication regulation.C-NFs with excellent lubrication performance can immediately reduce the coefficient of friction(COF)despite applying a weak electric potential(1.5 V).Moreover,polyethylene glycol 400(PEG400)containing 5.0 wt%C-NFs remained responsive to electrical stimulation under the intermittent voltage application with an average coefficient of friction(ACOF)reduction of 20.8%over *** intelligent lubrication regulation of C-NFs under an external electric field(EEF)mainly depends on the orderly arranged double-electric adsorption film of ion canopy-adsorbed carbon spheres(CSs).The intermittent electrical application can continuously reinforce the adsorption film and its durability for real-time controlling the sliding ***-stimulation-responsive intelligent lubricants provide a new technical support for realizing intelligent stepless control of devices.

关键词： solvent-free carbon spherical nanofluids(C-NFs) intelligent lubrication regulation lubrication performance adsorption film

Microphase Separation of Semiflexible Ring Diblock Copolymers

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Chinese Journal of Polymer Science 2024年第2期42卷 267-276,I0011页

作者： Dan-Yan Qin sheng-Da Zhao Zhi-Xin Liu Jing zhang Xing-Hua zhang School of Physical Science and Engineering Beijing Jiaotong UniversityBeijing100044China

Aiming at the difficult problem of solving the conformation statistics of complex polymers, this study presents a novel and concise conformation statistics theoretical approach based on Monte Carlo and Neural Network method. This method offers a new research idea for investigating the conformation statistics of complex polymers, characterized by its simplicity and practicality. It can be applied to more complex topological structure, more higher degree of freedom polymer systems with higher dimensions, theory research on dynamic self-consistent field theory and polymer field theory, as well as the analysis of scattering experimental data. The conformation statistics of complex polymers determine the structure and response properties of the system. Using the new method proposed in this study, taking the semiflexible ring diblock copolymer as an example, Monte Carlo simulation is used to sample this ring conformation to construct the dataset of polymer. The structure factor describing conformation statistics are expressed as continuous functions of structure parameters by neural network supervised learning. This is the innovation of this work. As an application, the structure factors represented by neural networks were introduced into the random phase approximation theory to study the microphase separation of semiflexible ring diblock copolymers. The influence of the ring's topological properties on the phase transition behavior was pointed out.

关键词： Ring copolymer Diblock copolymers Semiflexible The order-disorder transition

Electrochemical synthesis of trimetallic nickel-iron-copper nanoparticles via potential-cycling for high current density anion exchange membrane water-splitting applications

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Journal of Energy Chemistry 2024年第2期89卷 535-542,I0012页

作者： Ziqi zhang sheng Wan Hanbo Wang Jinghan He Ruige zhang Yuhang Qi Haiyan Lu State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of ChemistryJilin UniversityChangchun 130012JilinChina

Hydrogen is known for its elevated energy density and environmental compatibility and is a promising alternative to fossil *** water electrolysis utilizing renewable energy sources has emerged as a means to obtain high-purity ***,electrocatalysts used in the process are fabricated using conventional wet chemical synthesis methods,such as sol-gel,hydrothermal,or surfactantassisted approaches,which often necessitate intricate pretreatment procedures and are vulnerable to post-treatment ***,this study introduces a streamlined and environmentally conscious one-step potential-cycling approach to generate a highly efficient trimetallic nickel-iron-copper electrocatalyst in situ on nickel *** synthesized material exhibited remarkable performance,requiring a mere 476 mV to drive electrochemical water splitting at 100 mA cm^(-2)current density in alkaline ***,this material was integrated into an anion exchange membrane watersplitting device and achieved an exceptionally high current density of 1 A cm^(-2)at a low cell voltage of2.13 V,outperforming the noble-metal benchmark(2.51 V).Additionally,ex situ characterizations were employed to detect transformations in the active sites during the catalytic process,revealing the structural transformations and providing inspiration for further design of electrocatalysts.

关键词： Electrocatalytic water splitting Hydrogen evolution reaction Oxygen evolution reaction Electrochemical synthesis Anion exchange membrane

Giant reversible barocaloric effects with high thermal cycle stability in epoxybonded(MnCoGe)_(0.96)(CuCoSn)_(0.04) composite

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International Journal of Minerals,Metallurgy and Materials 2024年第11期31卷 2528-2534页

作者： Yafei kuang Kun Tao Bo Yang Peng Tong Yan zhang Zhigang Sun Kewei zhang Dunhui Wang Jifan Hu Liang Zuo College of Materials Science and Engineering Taiyuan University of Science and TechnologyTaiyuan 030024China Shanxi Key Laboratory of Magnetic and Electric Functional Materials and Applications Taiyuan University of Science and TechnologyTaiyuan 030024China Key Laboratory of Materials Physics Institute of Solid State PhysicsChinese Academy of SciencesHefei 230031China Key Laboratory for Anisotropy and Texture of Materials(Ministry of Education) School of Materials Science and EngineeringNortheastern UniversityShenyang 110819China School of Electronics and Information Hangzhou Dianzi UniversityHangzhou 310018China

Hexagonal MnMX-based(M=Co or Ni,X=Si or Ge)alloys exhibit giant reversible barocaloric ***,giant volume expansion would result in the as-cast MnMX ingots fragmenting into powders,and inevitably bring the deterioration of mechanical properties and *** fragmentation can bring degradation of structural transformation entropy change during cyclic application and removal of *** this paper,giant reversible barocaloric effects with high thermal cycle stability can be achieved in the epoxy bonded(MnCoGe)0.96(CuCoSn)0.04 *** reversible isothermal entropy change of 43.0 J·kg^(−1)·K^(−1) and adiabatic temperature change from barocaloric effects(ΔT_(BCE))of 15.6 K can be obtained within a wide temperature span of 30 K at 360 MPa,which is mainly attributed to the integration of the change in the transition temperature driven by pressure of−101 K·GPa^(−1) and suitable thermal hysteresis of 11.1 ***,the variation of reversibleΔ_(TBCE) against the applied hydrostatic pressure reaches up to 43 K·GPa^(−1),which is at the highest level among the other reported giant barocaloric *** importantly,after 60 thermal cycles,the composite does not break and the calorimetric curves coincide well,demonstrating good thermal cycle stability.

关键词： barocaloric effects cycle stability reversible isothermal entropy change reversible adiabatic temperature change MnCoGe alloys

Linking of Chemical Reactions and Silica Nanoparticle Contact Using Synthetic Helical Molecules

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Chinese Journal of Chemistry 2024年第15期42卷 1781-1796页

作者： sheng zhang Ming Bao Mieko Arisawa Masahiko Yamaguchi state Key Laboratory of Fine Chemicals Dalian University of TechnologyDalianLiaoning 116024China Department of Bioscience and Biotechnology Graduate Schoolof Bioresource and Bioenvironmental SciencesKyushu UniversityFukuoka 819-0395Japan

Biological cells exhibit diverse phenomena induced through linking of chemical reactions of molecules and solid surface *** is then a significant topic in the field of chemistry to study phenomena induced through this linking using synthetic systems,which can promote our understanding of biological phenomena and can be applied to the development of novel *** nanoparticles(sNPs),which are synthetic inorganic materials,are attractive for such purposes,because of their following characteristics:they can adsorb large amounts of molecules on their surfaces,they can aggregate through contact between SNPs as well as contact between molecules and SNPs,and the molecules can be easily removed from solutions by *** contact of sNP surfaces with molecules then affects chemical reactions of molecules and also behaviors of *** article describes systems derived from synthetic helical molecules and sNPs,which exhibit notable phenomena including selective adsorption and molecular recognition,equilibrium shift,step kinetics with induction period,precipitation with flow and sweeping,and disaggregation and desorption by sonication,in which the high affinity of helical molecules with sNP surfaces plays important *** models that explain the phenomena are *** applications are also discussed,including the separation of molecules,capture of intermediates,the storage and release of molecules,equilibrium shift,clocking,and the translation of mechanical stimulations into chemical reactions.

关键词： Silica nanoparticles Chemical reactions Molecular recognition Linking Nanoparticles Adsorption Chirality Precipitation

Neurotoxicity of tetramethylammonium ion on larval and juvenile zebrafish:Effects on neurobehaviors and multiple biomarkers

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Journal of Environmental Sciences 2024年第9期143卷 138-147页

作者： Ruixin zhang Rui Wang Jiajun Chang GDaniel sheng Daqiang Yin Key Laboratory of Yangtze River Water Environment Ministry of EducationCollege of Environmental Science and EngineeringTongji UniversityShanghai 200092China Shanghai Institute of Pollution Control and Ecological Security Shanghai 200092China

Tetramethylammonium hydroxide(TMAH)is an important compound that utilized and released by the rapidly expanding semiconductor industry,which could hardly be removed by the conventional wastewater treatment *** a cholinergic agonist,the tetramethylammonium ion(TMA^(+))has been reported to induce toxicity to muscular and respiratory systems of mammals and human,however the toxicity on aquatic biota remains poorly *** investigated the neurotoxic effects of TMA^(+)exposure on zebrafish,based on neurobehavior tests and a series of *** inhibitions on the swimming distance of zebrafish larvae were observed when the exposure level exceeded 50 mg/L,and significant alterations on swimming path angles(straight and deflective movements)occurred even at 10 mg/*** tested neurobehavioral endpoints of zebrafish larvae were significantly positively correlated with reactive oxygen species(ROS)and malondialdehyde(MDA),significantly negatively related with the activities of antioxidant enzymes,but not significantly correlated with the level of acetylcholinesterase(AChE).Such relationship indicates that the observed neurotoxic effects on swimming behavior of zebrafish larvae is mainly driven by oxidative stress,rather than the alterations of *** the highest exposure concentration(200 mg/L),TMA^(+)evoked more severe toxicity on zebrafish juveniles,showing significantly stronger elevation on the MDA activity,and greater inhibitions on the activities of antioxidant enzymes and ACh E,suggesting juveniles were more susceptible to TMA^(+)exposure than larval zebrafish.

关键词： Tetramethylammonium Zebrafish Oxidative stress Acetylcholinesterase Neurobehavior

Experimental study on the effect of flexible joints of a deep-buried tunnel across an active fault under high in-situ stress conditions

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Underground Space 2024年第6期19卷 189-207页

作者： Jiawei zhang Zhen Cui Qian sheng Wanhua Zhao Liang Song State Key Laboratory of Geomechanics and Geotechnical Engineering Institute of Rock and Soil MechanicsChinese Academy of SciencesWuhan 430071China School of Civil Engineering and Architecture Wuhan Polytechnic UniversityWuhan 430023China

During dislocation,a tunnel crossing the active fault will be damaged to varying degrees due to its permanent stratum *** previous studies did not consider the influence of the tunnel’s deep burial and the high in-situ stress,so the results were not entirely *** this paper,the necessity of solving the anti-dislocation problem of deep-buried tunnels is systemically *** the model test of tunnels across active faults,the differences in failures between deep-buried tunnels and shallow-buried tunnels were compared,and the dislocation test of deep-buried segmental tunnels was carried out to analyze the external stress change,lining strain,and failure mode of *** results are as follows.(1)The overall deformation of deep-buried and shallow-buried tunnels is both *** failure mode of deep-buried tunnels is primarily characterized by shear and tensile failure,resulting in significant compressive deformation and a larger damaged *** contrast,shallow-buried tunnels mainly experience shear failure,with the tunnel being sheared apart at the fault crossing,leading to more severe damage.(2)After the segmental structure design of the deep-buried tunnel,the‘‘S”deformation pattern is transformed into a‘‘ladder”pattern,and the strain of the tunnel and the peak stress of the external rock mass are reduced;therefore,damages are significantly mitigated.(3)Through the analysis of the distribution of cracks in the tunnel lining,it is found that the tunnel without a segmental structure design has suffered from penetrating failure and that cracks affect the entire *** cracks in a flexible segmental tunnel affect about 66.6%of the entire length of the tunnel,and cracks in a tunnel with a short segmental tunnel only affect about 33.3%of the entire length of the ***,a deep-buried tunnel with a short segmental tunnel can yield a better anti-dislocation effect.(4)By comparing the shallow-buried segmental tunnel in previous

关键词： Tunnel project Crossing active fault High in-situ stress Segmental structure design Flexible joint