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A body mass index ≥25 kg/m2 is associated with a poor prognosis in patients with acute pancreatitis: a study of Japanese patients

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Hepatobiliary & Pancreatic Diseases International 2017年第6期16卷 645-651页

作者： Tsukasa Ikeura kota Kato Makoto Takaoka Masaaki Shimatani Masanobu Kishimoto Kenichiro Nishi Shuji Kariya Kazuichi Okazaki Department of Gastroenterology and Hepatology Kansai Medical University 2-5-1 Shinmachi Hirakata Osaka 5731010 Japan Department of Emergency and Critical Care Medicine Kansai Medical University 2-5-1 Shinmachi Hirakata Osaka 5731010 Japan Department of Anesthesiology Kansai Medical University 2-5-1 Shinmachi Hirakata Osaka 5731010 Japan Department of Radiology Kansai Medical University 2-5-1 Shinmachi Hirakata Osaka 5731010 Japan

BACKGROUND:In Asian population, there is limited infor mation on the relevance between obesity and poor outcomes in acute pancreatitis(AP). The objective of this study was to examine the clinical impact of obesity based on body mass index(BMI) on prognosis of AP in Japanese ***:A total of 116 patients with AP were enrolled in this study. Univariate and multivariate logistic regression analyses were performed to examine relations between BMI and patients’ outcomes. Additionally, to investigate whether including obesity as a prognostic factor improved the predic tive accuracy of a Japanese prognostic factor score(PF score)a receiver-operating characteristic(ROC) curve analysis of mortality was ***:Multiple logistic regression analyses revealed that BMI ≥25 kg/m2was associated with a significant higher mor tality [odds ratio(OR)=15.8; 95% confidence interval(CI):1.1-227; P=0.043]. The area under the ROC curve(AUC) for the combination of PF score and BMI ≥25 kg/m2(AUC=0.881;95% CI:0.809-0.952) was higher than that for the PF score alone(AUC=0.820; 95% CI:0.713-0.927)(P=0.034).CONCLUSIONS:The negative impact of a high BMI on the prognosis of AP was confirmed in a Japanese population Including BMI ≥25 kg/m2 as an additional parameter to PF score enhanced the predictive value of the PF score for AP-related mortality.

关键词： acute pancreatitis obesity body mass index severity criteria prognostic factor score

Glucose-regulated protein precursor （GRP78） and tumor rejection antigen （GP96） are unique to hamster caput epididymal spermatozoa

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Asian Journal of Andrology 2010年第3期12卷 344-355,I0009页

作者： Duvvuri Butchi Kameshwari Satish Bhande Curam Sreenivasacharlu Sundaram Venkatesh kota Archana B. Siva Sisinthy Shivaji Centre for Cellular and Molecular Biology Hyderabad 500007 India

The immotile testicular mammalian spermatozoon gets transformed into a motile spermatozoon during ＇epididymal maturation＇. During this process, the spermatozoa transit from the caput to the cauda epididymis and undergo a number of distinct morphological, biophysical and biochemical changes, including changes in protein composition and protein modifications, which may be relevant to the acquisition of motility potential. The present proteome-based study of the hamster epididymal spermatozoa of caput and cauda led to the identification of 113 proteins spots using Matrix- assisted laser desorption/ionization tandem mass spectrometry （MALDI-MS/MS） analysis. Comparison of these 113 protein spots indicated that 30 protein spots （corresponding to 20 proteins） were significantly changed in intensity. Five proteins were increased and eleven were decreased in intensity in the cauda epididymal spermatozoa. In addition, two proteins, glucose-regulated protein precursor （GRP78） and tumor rejection antigen （GP96）, were unique to the caput epididymal spermatozoa, while one protein, fibrinogen-like protein 1, was unique to cauda epididymal spermatozoa. A few of the five proteins, which increased in intensity, were related to sperm metabolism and ATP production during epididymal maturation. The changes in intensity of a few proteins such as ERp57, GRP78, GP96, Hsp60, Hsp70, and dihydrolipoamide S-acetyltransferase were validated by immunoblotting. The present study provides a global picture of the changes in protein composition occurring during hamster sperm epididymal maturation, besides being the first ever report on the proteome of hamster spermatozoa.

关键词： epididymis hamster motility proteome spermatozoa

Evaluation of cytotoxic and anti-tumor activity of partially purified serine protease isolate from the Indian earthworm Pheretima posthuma

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Asian Pacific Journal of Tropical Biomedicine 2013年第11期3卷 896-901页

作者： Mahendra Kumar Verma Francies Xavier Yogendra Kumar Verma kota Sobha Department of Biotechnology R.V.R.& J.C.College of Engineering Department of Microbiology All India Institute of Medical Sciences

Objective:To isolate,partially purify and evaluate the cytotoxic and antitumor activity of a serine protease from the chosen Indian earthworm Pheretima ***:Whole animal extract was prepared and purified its protein constituents by size and charge based chromatographic separation techniques using Sephadex G-50 and DEAE-Cellulose resin *** molecular weight of the protein isolate was determined and analyzed for its cytotoxic property against Vero cells in different dilutions(1:20 and 1:40)and anti-tumor activity by MTT assay(a colorimetric assay)using breast cancer cell line MCF-7,with tamoxifen as ***:One of the protein constituents after purification was characterized as serine protease by Caseinolytic plate diffusion *** molecular weight of this purified isolate was determined,by SDS-PAGE analysis with standard protein ladder,as of 15 *** performed tests suggested that the 15kDa fraction has potent cytotoxic activity and satisfactory antitumor activity as well in ***:Exact molecular mechanism of the cytotoxic and antitumor activities is yet to be explored and currently we are working on ultra-purification and biophysical characterization of this *** investigation into the mechanism(s)of cytotoxic and antitumor activities at molecular level would be useful in treatment of various classes of cancer and viral infections in future.

关键词： Earthworm fibrinolytic enzyme Cytotoxicity Antitumor activity Serine protease Minimum inhibitory concentration

Guidance, navigation, and control of Hayabusa2 touchdown operations

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Astrodynamics 2020年第4期4卷 393-409页

作者： Fuyuto Terui Naoko Ogawa Go Ono Seiji Yasuda Tetsuya Masuda kota Matsushima Takanao Saiki Yuichi Tsuda Institute of Space and A stronautical Science Japan A erospace Exploration AgencyKanagawa 252-5210Japan The 3nd Space Technology Department Space System DirisionNEC CorporationTokyo 183-8501Japan

Hayabusa2 is a Japanese sample return mission from the asteroid *** Hayabusa2 spacecraft was launched on 3 December 2014 and arrived at Ryugu on 27 June *** stayed there until December 2019 for in situ observation and soil sample collection,and will return to the Earth in November or December *** the stay,the spacecraft performed the first touchdown operation on 22 February 2019 and the second touchdown on 11 July 2019,which were both completed *** the surface of Ryugu is rough and covered with boulders,it was not easy to find target areas for *** were several technical challenges to overcome,including demanding guidance,navigation,and control accuracy,to realize the touchdown *** this paper,strategies and technical details of the guidance,navigation,and control systems are *** flight results prove that the performance of the systems was satisfactory and largely contributed to the success of the operation.

关键词： small body exploration touchdown astrodynamics guidance navigation control

Effects of docosahexaenoic acid or arachidonic acid supplementation on the behavior of cardiomyocytes derived from human pluripotent stem cells

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BIOCELL 2023年第5期47卷 1095-1106页

作者： MIZUNA YANO kota HIROI TETSUYA YUASA KENJI INOUE OSAMU YAMAMOTO TAKAO NAKAMURA DAISUKE SATO ZHONGGANG FENG Graduate School of Science and Engineering Yamagata UniversityYonezawa9928510Japan Faculty of Health Sciences Japan Healthcare UniversitySapporo0620053Japan

Background:Human heart changes its energetic substrates from lactate and glucose to fatty acids during the neonatal *** the lack of fatty acids in media for the culture of cardiomyocytes derived from human pluripotent stem cells(hiPS-CM),researchers have supplemented mixtures of fatty acids to hiPS-CM and reported the enhancement in the maturation of *** our previous studies,we separately supplemented two polyunsaturated fatty acids(PUFAs),docosahexaenoic acid(DHA)or arachidonic acid(AA),to rat fetal cardiomyocytes and found that the supplementations upregulated the expressions of mRNAs for cardiomyocyte differentiation,fatty acid metabolism,and cellular *** enhancement in cellular contractility was attributed to the improvement in intercellular connection rather than a direct enhancement of the contractile ***:This study reports the successive results of the effects of DHA or AA supplementation on *** addition to the contractile force and mRNA measurements used in the previous study,we further investigated the effect of different cellular aggregations on the contractile force output by means of finite element analysis,measured glucose and fatty acids metabolites,and assessed cTNT and MLC2v expressions through immunofluorecsence ***:It showed that the sole supplementation of albumin-conjugated DHA or AA can be taken up by hiPS-CM without other uptake-enhancing factors,and the supplementations may activate the CD36_ERRγmetabolic *** or AA supplementation increased the cellular contractile ratio on collagen gels and AA supplementation stimulated hiPS-CM aggregation to form cellular *** enhancement effect on the hiPS-CM contractile force was modest since the increase in contractile force was not *** supplementation was more effective than DHA supplementation because it significantly upregulated mRNA expressions of P300 and ***,finite element analysis showed that the formati

关键词： Human iPS cells Cardiomyocytes Polyunsaturated fatty acid mRNA expression Contractility Fatty acid metabolism

Neurosteroids as stress modulators and neurotherapeutics: lessons from the retina

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Neural Regeneration Research 2023年第5期18卷 1004-1008页

作者： Yukitoshi Izumi Makoto Ishikawa Toru Nakazawa Hiroshi Kunikata kota Sato Douglas F.Covey Charles F.Zorumski Department of Psychiatry and Taylor Family Institute for Innovative Psychiatric Research Washington University School of MedicineSt.LouisMOUSA Department of Ophthalmic Imaging and Information Analytics Tohoku University Graduate School of MedicineSendaiJapan Department of Ophthalmology Tohoku University Graduate School of MedicineSendaiJapan Department of Retinal Disease Control Tohoku University Graduate School of MedicineSendaiJapan Department of Advanced Ophthalmic Medicine Tohoku University Graduate School of MedicineSendaiJapan Department of Developmental Biology Washington University School of MedicineSt.LouisMOUSA

Neurosteroids are rapidly emerging as important new therapies in neuropsychiatry, with one such agent, brexanolone, already approved for treatment of postpartum depression, and others on the horizon. These steroids have unique properties, including neuroprotective effects that could benefit a wide range of brain illnesses including depression, anxiety, epilepsy, and neurodegeneration. Over the past 25 years, our group has developed ex vivo rodent models to examine factors contributing to several forms of neurodegeneration in the retina. In the course of this work, we have developed a model of acute closed angle glaucoma that involves incubation of ex vivo retinas under hyperbaric conditions and results in neuronal and axonal changes that mimic glaucoma. We have used this model to determine neuroprotective mechanisms that could have therapeutic implications. In particular, we have focused on the role of both endogenous and exogenous neurosteroids in modulating the effects of acute high pressure. Endogenous allopregnanolone, a major stress-activated neurosteroid in the brain and retina, helps to prevent severe pressure-induced retinal excitotoxicity but is unable to protect against degenerative changes in ganglion cells and their axons under hyperbaric conditions. However, exogenous allopregnanolone, at a pharmacological concentration, completely preserves retinal structure and does so by combined effects on gamma-aminobutyric acid type A receptors and stimulation of the cellular process of macroautophagy. Surprisingly, the enantiomer of allopregnanolone, which is inactive at gamma-aminobutyric acid type A receptors, is equally retinoprotective and acts primarily via autophagy. Both enantiomers are also equally effective in preserving retinal structure and function in an in vivo glaucoma model. These studies in the retina have important implications for the ongoing development of allopregnanolone and other neurosteroids as therapeutics for neuropsychiatric illnesses.

关键词： allopregnanolone autophagy enantiomers excitotoxicity gamma-aminobutyric acid type A receptors glaucoma optic nerve oxysterols

Intussusception due to hematogenous metastasis of hepatocellular carcinoma to the small intestine:A case report

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World Journal of Gastroenterology 2020年第42期26卷 6698-6705页

作者： Taro Mashiko Yoshihito Masuoka Akira Nakano kota Tsuruya Shunji Hirose Kenichi Hirabayashi Tatehiro Kagawa Toshio Nakagohri Department of Gastroenterological Surgery Tokai University School of MedicineIsehara 2591193KanagawaJapan Department of Gastroenterology Tokai University School of MedicineIsehara 2591193KanagawaJapan Department of Pathology Tokai University School of MedicineIsehara 2591193KanagawaJapan

BACKGROUND The commonest sites of extrahepatic metastases from hepatocellular carcinoma(HCC)are the lungs,bones,adrenal glands,and regional lymph *** metastasis to the gastrointestinal(GI)tract is a rare condition in patients with HCC,and the prognosis is usually *** report,herein,an extremely rare case of a patient with intussusception due to hematogenous metastasis of HCC to the ileum and his long-term survival with multidisciplinary *** SUMMARY The patient was a 71-year-old man with a history of chronic hepatitis B,who had undergone three surgeries for *** was treated with sorafenib for peritoneal metastases of *** was admitted to our hospital with chief complaints of abdominal pain and *** contrast-enhanced computed tomography imaging revealed a small intestinal tumor,presenting with intussusception and small bowel *** treatment was started,but due to repeated exacerbation of symptoms,surgery was planned on the 28th d of *** ileal resection without reducing the intussusception and end-to-end anastomosis was *** histological examination,tumor cells were not observed on the serosal surface,but intravascular invasion of tumor cells was *** was positive for immunohistochemical markers,and a diagnosis of hematogenous metastasis of HCC to the ileum was *** remains alive 82 mo after the first *** Prognosis of HCC patients with GI tract metastasis is usually poor,but in some cases,multidisciplinary therapy may prolong survival.

关键词： Hepatocellular carcinoma Hematogenous metastases Extrahepatic metastasis Small intestinal metastasis Intussusception Case report

Benign esophageal stricture after thermal injury treated with esophagectomy and ileocolon interposition

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World Journal of Gastroenterology 2014年第27期20卷 9205-9209页

作者： Toshihiro Kitajima kota Momose Seigi Lee Shusuke Haruta Hisashi Shinohara Masaki Ueno Takeshi Fujii Harushi Udagawa Department of Digestive Surgery Toranomon Hospital 2-2-2 Toranomon Minato-ku Tokyo 105-8470 Japan Department of Pathology Toranomon Hospital 2-2-2 Toranomon Minato-ku Tokyo 105-8470 Japan

Thermal injuries of the esophagus are rare causes of benign esophageal stricture, and all published cases were successfully treated with conservative management. A 28-year-old Japanese man with a thermal esophageal injury caused by drinking a cup of hot coffee six months earlier was referred to our hospital. The hot coffee was consumed in a single gulp at a party. Although the patient had been treated conservatively at another hospital, his symptoms of dysphagia gradually worsened after discharge. An upper gastrointestinal endoscopy and computed tomography revealed a pinhole like area of stricture located 19 cm distally from the incisors to the esophagogastric junction, as well as circumferential stenosis with notable wall thickness at the same site. The patient underwent a thoracoscopic esophageal resection with reconstruction using ileocolon interposition. The pathological findings revealed wall thickening along the entire length of the esophagus, with massive fibrosis extending to the muscularis propria and adventitia at almost all levels. Treatment with balloon dilation for long areas of stricture is generally difficult, and stent placement in patients with benign esophageal stricture, particularly young patients, is not yet widely accepted due to the incidence of late adverse events. Considering the curability and qualityof-life associated with a long expected prognosis, we determined that surgery was the best treatment option for this young patient. In this case, we decided to perform an esophagectomy and reconstruction with ileocolon interposition in order to preserve the reservoir function of the stomach and to avoid any problems related to the reflux of gastric contents. In conclusion, resection of the esophagus is a treatment option in patients with benign esophageal injury, especially in cases involving young patients with refractory esophageal stricture. In addition, ileocolon interposition may help to improve the quality-of-life of patients.

关键词： Thermal injury Benign esophageal stricture Esophageal resection Ileocolon interposition Video-assisted thoracic surgery

Alkali Treatment of Commercial Silicoaluminophosphate Molecular Sieves (SAPO<sub>-</sub>34) Enhances the Water Adsorption and Desorption Properties

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Journal of Encapsulation and Adsorption Sciences 2019年第4期9卷 149-158页

作者： Masahiro Katoh kota Horiuchi Ayaka Satoh kota Aoyagi Shigeru Sugiyama Department of Applied Chemistry Graduate School of Technology Industrial and Social Sciences Tokushima University Tokushima Japan Department of Chemical Science and Technology Tokushima University Tokushima Japan

Commercial silicoaluminophosphate molecular sieves (SAPO-34) received alkali treatment with either NaOH (0.2, 0.01, 0.005, or 0.001 M) or NH4OH (0.005 M). Treatment with NaOH (0.005 M) increased the water adsorption initial rate of SAPO-34 by 1.4-fold. The alkali treatment introduced Na+ adsorption sites into the SAPO-34. The desorption ratio (adsorption at 30°C and desorption at 100°C) was 88.2% higher than the original rate (84.3%). On the other hand, after alkali treatment of SAPO-34 using NH4OH (0.005 M), calcination resulted in the highest desorption ratio at 91.3%. When combined with calcination, alkali treatment with NH4OH introduced H+adsorption sites into SAPO-34, H+ adsorption sites feature low levels of interaction with water, which enhanced the desorption ratio, but decreased the initial adsorption rate. These results indicate that treating commercial SAPO-34 with 0.005 M NaOH enhances both the adsorption and desorption behaviors.

关键词： Alkali Treatment Silicoaluminophosphate Molecular Sieves Water Adsorption Desorption