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MK615 inhibits pancreatic cancer cell growth by dual inhibition of Aurora A and B kinases

World Journal of Gastroenterology 2008年第9期14卷 1378-1382页

作者： Toshie Okada Tokihiko sawada Tatsushi Osawa Masakazu Adachi Keiichi Kubota Second Department of Surgery Dokkyo University School of Medicine Kitakobayashi 880MibuShimotsugaTochigi 321-0293Japan Second Department of Surgery Dokkyo University School of Medicine Kitakobayashi 880MibuShimotsuga Tochigi 321-0293Japan Japan Apricot Co.Ltd.1-11-7 MidorichoTakasakiGunma 370-0073 Japan Japan Apricot Co. Ltd.1-11-7 MidorichoTakasakiGunma 370-0073 Japan Second Department of Surgery Dokkyo University School of MedicineKitakobayashi 880Mibu ShimotsugaTochigi 321-0293Japan

AIM:To investigate the anti-neoplastic effect of MK615, an anti-neoplastic compound isolated from Japanese apricot, against human pancreatic cancer cells in vitro. METHODS: Three human pancreatic cancer cell lines PANC-1, PK-1, and PK45H were cultured with MK615 at concentrations of 600, 300, 150, and 0 μg/mL. Growth inhibition was evaluated by cell proliferation assay, and killing activity was determined by lactate dehydrogenase (LDH) assay. Expression of Aurora A and B kinases was detected by real-time polymerase chain reaction (PCR) and Western blotting. Cell cycle stages were evaluated by flow cytometry. RESULTS: The growth inhibitory rates of MK615 at 150, 300, and 600 μg/mL were 2.3% ± 0.9%, 8.9% ± 3.2% and 67.1% ± 8.1% on PANC1 cells, 1.3% ± 0.3%, 8.7% ± 4.1% and 45.7 ± 7.6% on PK1 cells, and 1.2 ± 0.8%, 9.1% ± 2.1% and 52.1% ± 5.5% on PK45H cells, respectively (P <0.05). The percentage cytotoxicities of MK615 at 0, 150, 300, and 600 μg/mL were 19.6% ± 1.3%, 26.7% ± 1.8%, 25.5% ± 0.9% and 26.4% ± 0.9% in PANC1 cells, 19.7% ± 1.3%, 24.7% ± 0.8%, 25.9% ± 0.9% and 29.9% ± 1.1% in PK1 cells, and 28.0% ± 0.9%, 31.2% ± 0.9%, 30.4% ± 1.1% and 35.3 ± 1.0% in PK45H cells, respectively (P < 0.05). Real-time PCR and Western blotting showed that MK615 dually inhibited the expression of Aurora A and B kinases. Cell cycle analysis revealed that MK615 increased the population of cells in G2/M phase. CONCLUSION: MK615 exerts an anti-neoplastic effect on human pancreatic cancer cells in vitro by dual inhibition of Aurora A and B kinases.

关键词： Japanese apricot Aurora kinases Pancreatic cancer Cell cycle

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Third-look endoscopy prevents delayed bleeding after endoscopic submucosal dissection under antithrombotic therapy

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World Journal of Gastroenterology 2020年第41期26卷 6475-6487页

作者： Ryosuke Ikeda Kingo Hirasawa Chiko Sato Yuichiro Ozeki Atsushi sawada Masafumi Nishio Takehide Fukuchi Ryosuke Kobayashi Makomo Makazu Masataka Taguri Shin Maeda Endoscopy Division Yokohama City University Medical CenterYokohama 232-0024Japan Endoscopy Division Yokohama Medical University Center HospitalYokohama 232-0024Japan Department of Data Science Yokohama City University School of Data ScienceYokohama 236-0004Japan Division of Gastroenterology Yokohama City University Graduate School of MedicineYokohama 236-0004Japan

BACKGROUND Postoperative delayed bleeding(PDB) after gastric endoscopic submucosal dissection(ESD) is the most common adverse event in patients receiving antithrombotics even with second-look endoscopy. Moreover, with the increasing prevalence of cardiovascular and cerebrovascular diseases in an aging population with associated lifestyle-related diseases, an increasing number of patients receive antithrombotics. Several attempts have been made to prevent PDB in aging population;however, a consensus has yet to be *** To examine the efficacy of third-look endoscopy(TLE) for PDB *** One hundred patients with early gastric neoplasms receiving antithrombotics were prospectively enrolled and subjected to ESD with TLE between February 2017 and July 2019. The primary endpoint was PDB rate, which was compared with our preset threshold. Furthermore, we divided the bleeding period into early-and late-onset PDB(E-PDB and L-PDB, respectively) and analyzed its rate. As a secondary analysis, we compared PDB rates with those of a historical control group, using propensity score matching, and calculated the PDB rates per antithrombotic agent use in each *** In total, 96 patients and 114 specimens were finally evaluated. The overall PDB rate was 7.9%(9/114) [90%CI: 4.7-13.1, P = 0.005], while the late-and early-onset PDB rates(L-PDB and E-PDB) were 5.3% [90%CI: 2.7-9.9, P < 0.0001] and 2.6% [90%CI: 1.1-6.4, P = 0.51], respectively. Propensity score matching generated 58 matched pairs for TLE and control groups. No differences were found in overall PDB incidence(10.3% vs 20.7%, P = 0.12), whereas L-PDB occurrence significantly differed(5.2% vs 17.2%, P = 0.04) between groups. Considering antithrombotics' use, the overall PDB rate was higher for direct oral anticoagulants and multiple antithrombotics in the control group, while L-PDB incidence was lower in the TLE group for these agents(8.7% vs 23.1% and 5.0% vs 29.4%, respectively).CONCLUSION TLE for g

关键词： Endoscopic submucosal dissection Postoperative delayed bleeding Third look endoscopy Antithrombotic agents Late phase bleeding Phase II

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Hepatocellular carcinoma with chronic B-type hepatitis complicated by autoimmune hemolytic anemia:A case report

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World Journal of Gastroenterology 2007年第32期13卷 4401-4404页

作者： Toshie Okada Keiichi Kubota Junji Kita Masato Kato Tokihiko sawada Department of Gastro-enterological Surgery Dokkyo University School of MedicineTochigiJapan

A 57-year-old man consulted a local hospital because of a persistent slight fever. At the age of 37 years he was diagnosed having B-type hepatitis, but left the liver dysfunction untreated. Twenty years later, he was diagnosed having chronic hepatitis B, hepatocellular carcinoma （HCC） and macrocytic anemia, and referred to our hospital for further investigation. A HCC with a maximum diameter of 5.2 cm was detected in segment 8. Results of blood tests included 1.8 mg/dL serum total bilirubin, 0.9 mg/dL bilirubin, less than 10 mg/dL haptoglobin, 7.9 g/dL hemoglobin, 130 fL MCV, and 14.5% reticuloo/tes. A bone marrow sample showed erythroid hyperplasia. The direct Coombs test gave a positive result. We diagnosed the anemia as autoimmmune hemolytic anemia （AIHA）, for which prednisolone could not be administered due to positivity for HBsAg and HBeAg. After preparation of washed blood cells for later transfusion, the patient underwent systematic resection of segment 8. The cut surface of the resected specimen demonstrated an encapsulated yellow-brownish tumor measuring 52 mmx 40 mm which was diagnosed pathologicaly as moderately differentiated HCC. On the 9th postoperative day, the patient＇s temperature rose to 38℃, and exacerbated hemolysis was observed. The maximum total bilirubin value was 5.8 mg/dL and minimum hemoglobin level was 4.6 g/dL. He tolerated this period without blood transfusion. Currently he is being followed up as an outpatient, and shows no signs of HCC recurrence or symptoms of anemia. AIHA associated with HBV infection has been described in only three previous cases, and the present case is the first in which surgery was performed for accompanying HCC.

关键词： Hepatocellular carcinoma B-type hepatitis Auto-immune hemolytic anemia

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Radiofrequency ablation therapy for malignant tumors

Radiofrequency ablation therapy for malignant tumors

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第三届全国肿瘤介入大会暨肿瘤介入进展学术研讨会

作者： Satoshi sawada Noboru Tanigawa Department of Radiology Kansai Medical University

Radiofrequency ablation is a miniraary invasive therapy that is used for various malignant tumors. In this lecture, we discuss the usefulness of percutaneous radiofrequency ablation (RFA) for painful metastatic bone tumors and introduce our clinical experiences of RFA for lung tumor and kidney. RFA for bone tumors: Subjects comprised 24 patients (28 lesions) with painful metastatic bone tumors who

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THE INFLUENCE OF MICROSTRUCTURAL INSTABILITY ON CREEP BEHAVIOR OF A NICKEL-BASE UDEMET 500 SUPERALLOY

THE INFLUENCE OF MICROSTRUCTURAL INSTABILITY ON CREEP BEHAVI...

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第一届国际机械工程学术会议

作者： Kota sawada Fujio Abe National Research Institute for Metals Japan

The creep deformation behavior and its relationship with the microstructural instability during creep were investigated for a nickel-base Udimet 500 superalloy via the creep testing at 800 and 900C for up to 22,000 hours. All of the creep curves under the different test conditions consist of very shorter transient creep and longer acceleration creep. In the tertiary creep region, the acceleration of creep rate occurs in two stages. Moreover, the microstructural evolutions such as the coarsening of βphase, the precipitation of βphase, grain boundary nature and internal nitridation attack were analyzed.

关键词： Creep Udimet 500 superalloy B phase

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New anti-proliferative agent,MK615,from Japanese apricot “Prunus mume” induces striking autophagy in colon cancer cells in vitro

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World Journal of Gastroenterology 2007年第48期13卷 6512-6517页

作者： Shozo Mori Tokihiko sawada Toshie Okada Tatsushi Ohsawa Masakazu Adachi Kubota Keiichi Second Department of Surgery Dokkyo University School of Medicine Japan Apricot Co. Ltd

AIM: To investigate the anti-neoplastic effects of MK615, an extract from the Japanese apricot (Prunus mume), against colon cancer cells. METHODS: Three colon cancer cell lines, SW480, COLO, and WiDr, were cultured with MK615. Growth inhibition was evaluated by cell proliferation assay and killing activity was determined by lactate dehydrogenase assay. Induction of apoptosis was evaluated by annexin Ⅴ flow cytometry. Morphological changes were studied by light and electron microscopy, and immunofluorescence staining with Atg8. RESULTS: MK615 inhibited growth and lysed SW480, COLO and WiDr cells in a dose-dependent manner. Annexin Ⅴ flow cytometry showed that MK615 induced apoptosis after 6 h incubation, at which point the occurrence of apoptotic cells was 68.0%, 65.7% and 64.7% for SW480, COLO, and WiDr cells, respectively. Light and electron microscopy, and immunofluorescence staining with Atg8 revealed that MK615 induced massive cytoplasmic vacuoles (autophagosomes) in all three cell lines. CONCLUSION: MK615 has an anti-neoplastic effect against colon cancer cells. The effect may be exerted by induction of apoptosis and autophagy.

关键词： Colon cancer Japanese apricot Prunus mume Autophagy Apoptosis MK615

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Supercapacitor Consisting of a Form Core Sandwich with Woven Carbon Fiber Skin

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Open Journal of Composite Materials 2015年第4期5卷 101-109页

作者： Akira Todoroki Tomohiro sawada Yoshihiro Mizutani Yoshiro Suzuki Department of Mechanical Sciences of Engineering Tokyo Institute of Technology Tokyo Japan Tokyo Institute of Technology Tokyo Japan

Structural capacitors are composite structures that function as energy storage capacitors. Parallel plate-type capacitors have the advantage of high voltage resistance, but are limited by low capacitance. An electric double-layer capacitor with a composite structure using a solid polymer electrolyte matrix with a glass fiber fabric separator has recently been developed. However, the solid polymer electrolyte caused the capacitor to possess high internal resistance. In the present study, a new design of supercapacitor using a form core sandwich with high water retention is proposed and experimentally investigated. Activated carbon sheets are used as electrodes on the form core sandwich to make a supercapacitor. Woven carbon fabric is used as lead wires of the supercapacitor. The resulting supercapacitor displays a low surface resistance of 810 Ωcm2 and high areal capacitance of 520 mF/cm2.

关键词： Composites Woven Carbon Fabric Capacitor Supercapacitor Sandwich Form Core

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Histopathological validation of magnifying endoscopy for diagnosis of mixed-histological-type early gastric cancer

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World Journal of Gastroenterology 2020年第36期26卷 5450-5462页

作者： Yuichiro Ozeki Kingo Hirasawa Ryosuke Kobayashi Chiko Sato Yoko Tateishi Atsushi sawada Ryosuke Ikeda Masafumi Nishio Takehide Fukuchi Makomo Makazu Masataka Taguri Shin Maeda Division of Endoscopy Yokohama City University Medical CenterYokohama 232-0024Japan Department of Pathology Yokohama City University Graduate School of MedicineYokohama 236-0004Japan Department of Data Science Yokohama City University School of Data ScienceYokohama 236-0004Japan Department of Gastroenterology Yokohama City University Graduate School of MedicineYokohama 236-0004Japan

BACKGROUND The undifferentiated-type(UDT)component profoundly affects the clinical course of early gastric cancers(EGCs).However,an accurate preoperative diagnosis of the histological types is *** date,few studies have investigated whether the UDT component within mixed-histological-type(MT)EGCs can be recognized *** To clarify the histopathological characteristics of the endoscopically-resected MT EGCs for investigating whether the UDT component could be recognized *** This was a single-center retrospective ***,we attempted to clarify the histopathological characteristics of the endoscopically-resected MT EGCs with emphasis on the UDT *** examination investigated each lesion’s UDT component:(1)Whole mucosal layer occupation of the UDT component;(2)UDT component exposure to the surface of the mucosa;and(3)existence of a clear border between the differentiated-type and UDT ***,preoperative endoscopic images with magnifying endoscopy with narrowband imaging(ME-NBI)were examined to identify whether the endoscopic UDT component finding was recognizable within the area where it was present in the histopathological *** preoperative biopsy results and comparative relationships between endoscopic and histopathological findings were also *** In the histopathological examination,the whole mucosal layer occupation of the UDT component and exposure of the UDT component to the mucosal surface were observed in 67.3%(33/49)and 79.6%(39/49)of samples,respectively.A clear distinction of the border between the differentiated-type and UDT components could not be drawn in 65.3%(32/49)of MT *** the endoscopic examination,the preoperative endoscopic images showed that only 24.5%(12/49)of MT EGCs revealed the UDT component within the area where it was present *** UDT predominance was the single significant factor associated with the

关键词： Early gastric cancer Endoscopic submucosal dissection Mixed-histologicaltype Undifferentiated-type Narrow-band imaging

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Motion reconstruction of the small carry-on impactor aboard Hayabusa2

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Astrodynamics 2020年第4期4卷 289-308页

作者： Takanao Saiki Yuya Mimasu Yuto Takei Manabu Yamada Hirotaka sawada K azunori Ogawa Naoko Ogawa Hiroshi Takeuchi Akira Miura Yuri Shimaki koji Wada Rie Honda Yasuhiro Yokota Kei Shirai Naruhisa Sano Hirohito Ohtsuka Go Ono Kent Yoshikawa Shota Kikuchi Chikako Hirose Yukio Yamamoto Takahiro Iwata Masahiko Arakawa Seiji Sugita Satoshi Tanaka Fuyuto Terui Makoto Yoshikawa Satoru Nakazawa Sei-ichiro Watanabe Yuichi Tsuda Japan Aerospace Exploration Agency Sagamihara 252-5210Japan Chiba Institute of Technology Narashino 275-0016Japan Kobe University K obe 657-8501Japan The Graduate University for Aduanced Studies Sagamihara 252-5210Japan Kochi University Kochi 780-8520Japan IHI Aerospace Co. LtdTomioka 370-2398Japan The University of Tokyo Tokyo 113-0033Japan Nagoya University Nagoya 464-8601Japan

Subsurface exploration is one of the most ambitious scientific objectives of the Hayabusa2 mission.A small device called small carry-on impactor(SCI)was developed to create an artificial crater on the surface of asteroid *** enables us to sample subsurface materials,which will provide a window to the *** physical properties of the resulting crater are also useful for understanding the internal structure of *** understanding of the crater and ejecta properties,including the depth of excavation of subsurface materials,requires accurate information on impact *** particular,the impact angle is a critical factor because it greatly influences the size and shape of the *** April 5,2019,the Hayabusa2 spacecraft deployed the SCI at 500 m of altitude above the asteroid *** SCI gradually reduced its altitude,and it shot a 2 kg copper projectile into the asteroid 40 min after *** the position of the released SCI is essential for determining the impact *** study describes the motion reconstruction of the SCI based on the actual operation *** results indicate that the SCI was released with high accuracy.

关键词： small body exploration Hayabusa2 kinetic impact artificial crater motion reconstruction

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Modeling and analysis of Hayabusa2 touchdown

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Astrodynamics 2020年第2期4卷 119-135页

作者： Kent Yoshikawa Hirotaka sawada Shota Kikuchi Naoko Ogawa Yuya Mimasu Go Ono Yuto Takei Fuyuto Terui Takanao Saiki Seiji Yasuda Kota Matsushima Tetsuya Masuda Yuichi Tsuda Japan Aerospace Exploration Agency Sagamihara252-5210Japan NEC Corporation Fuchu183-8501Japan

The Hayabusa2 asteroid explorer mission focuses principally on the touchdown and sampling on near-Earth asteroid 162173 ***2 successfully landed on its surface and ejected a projectile for sample collection on February 22,***2 later landed near a crater formed by an impactor and executed the sampling sequence again on July 11,*** a successful mission,a thorough understanding and evaluation of spacecraft dynamics during touchdown were *** most challenging aspect of this study was the modeling of such spacecraft phenomena as the dynamics of landing on a surface with unknown *** particular,a Monte Carlo analysis was used to determine the parameters of the operational design for the final descent and touchdown *** paper discusses the dynamical modeling of the simulation during the touchdown of Hayabusa2.

关键词： contact dynamics resistive force theory(RFT) astrodynamics Hayabusa2 planetary exploration

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