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世界核心医学期刊文摘（眼科学分册） 2005年第11期1卷 50-51页

作者： Mirshahi A. kohnen t. 张少娟 Depart.ent.of Opht.almology Johann Wolf gang Goethe University Theodor-Stern-Kai 7 60590 Frankfurt amMain Germany Dr.

Purpose: t. st.dy t.e effect.of microkerat.me suct.on on ocular st.uct.res dur ing LASIK. Design: Observat.onal, prospect.ve case series. Part.cipant.: t.ent. -one eyes of 11 pat.ent. wit. myopia or ast.gmat.c myopia (8 females, 3 males) were included. t.e mean pat.ent.age was 36.3 years (median, 37 years; range, 24 -48 years), and t.e mean spherical equivalent.was-5.03 diopt.rs(D) (median,-4 .63 D; range,-2.38 t. -8.38 D). Met.ods: We performed preoperat.ve and int.aop erat.ve A-scan ult.asonography during applicat.ion of suct.on using t.e Hansat.me microkerat.me (Bausch &Lomb Surgical,Munich, Germany) t. creat. corneal flaps during LASIK. We also performed preoperat.ve a nd post.perat.ve B-scan ult.asonography of t.e post.rior ocular segment.wit. sp ecial at.ent.on t. t.e presence and size of post.rior vit.eous det.chment.(PVD). Main Out.ome Measures: We measured changes in t.e axial lengt., ant.rior chambe r dept., lens t.ickness, and vit.eous dist.nce (dist.nce from t.e post.rior lens capsule t. t.e post.rior pole) during applicat.on of t.e microkerat.me suct.on ring and recorded new occurrences of or increases in t.e size of t.e PVD aft.r s urgery. Result.: t.e lens t.ickness decreased (mean change,-0.20 mm; P=0.001; 9 5%confidence int.rval [CI],-0.11 t.-0.30) in 18 eyes during applicat.on of t. e suct.on ring. t.e vit.eous dist.nce increased (mean change, 0.20 mm; P=0.004; 95%CI, 0.08-0.32) in 16 eyes. No st.t.st.cally significant.changes were found in t.e ant.rior chamber dept. (P=0.75) or axial lengt. (P=0.51). Aft.r surgery, 3 of 14 eyes (21.4%) experienced PVD t.at.did not.have echographic signs of PVD before surgery. Of 7 eyes wit. preoperat.ve PVD, t.e PVD enlarged in 1 eye (14. 3%). Conclusions: During applicat.on of microkerat.me suct.on, t.e lens t.ickne ss decreases, whereas t.e vit.eous dist.nce increases, suggest.ng ant.rior t.act.ion on t.e post.rior segment. t.e relat.onship bet.een t.e observed PVD and LASI K merit. furt.er invest.gat.on.

关键词： LASIK术近视性散光吸引作用晶体厚度眼轴长度前房深度后囊器制病例研究观察性

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鹰爪抓伤所致的巩膜和角膜裂伤伴虹膜脱出

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世界核心医学期刊文摘（眼科学分册） 2005年第10期1卷 39-40页

作者： Muller L. kohnen t. 杨建刚 Depart.ent.of Opht.almology Johann Wolfgang G oethe-University Theodor-Stern-Kai 7 60590 Frankfurt amMain Germany

Background: t. describe t.e visual rehabilit.t.on aft.r surgical t.eat.ent.of an ocular injury sust.ined during an at.ack by a bird of prey. Met.ods: A 51-ye ar-old woman who was at.acked by an eagle in a cage had a lacerat.on of cornea and sclera at.9 o’clock, an iris prolapse t.at.was jammed int. t.e scleral woun d, and a flat.ant.rior chamber wit. hyphema. t.e uncorrect.d visual acuit. was h and movement.. Surgical t.eat.ent.was performed immediat.ly and included iris re posit.oning and sut.ring, scleral and conjunct.val sut.ring, and t.ansscleral cr yot.erapy t. t.e ret.na. Result.: Eight.mont.s lat.r, t.e best.correct.d visual acuit. was 20/20. t.e ant.rior segment.showed a post.rior synechia in t.e area o f t.e iris repair and an almost.round pupil; t.e cryst.lline lens showed only a localized opacificat.on. Conclusions: Abird of prey, even t.ough caged, can inju re an eye in humans. Wit. immediat. surgical t.eat.ent. good visual rehabilit.t.on was achieved in t.is case. It.s hould be reit.rat.d t.at.spect.cles offer no prot.ct.on against.ocular t.auma in t.ese cases.

关键词：角膜裂虹膜脱出抓伤虹膜复位眼外伤冷凝术浅前房前房出血眼前节虹膜后粘连

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通过Scheimpflug照相评价房角支撑、虹膜固定及睫状沟植入的近视性有晶状体眼中人工晶体的位置

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世界核心医学期刊文摘（眼科学分册） 2005年第3期1卷 17-17页

作者： Baumeist.r M. Bühren J. kohnen t. 张少娟

t. examine post.perat.ve posit.onal st.bilit. of myopic phakic int.aocular len ses (IOLs). Prospect.ve, nonrandomized clinical st.dy. t.e st.dy included 46 eye s which received an ant.rior chamber angle support.d (Bausch &Lomb NuVit.; 10 eyes) , ant.rior chamber iris fixat.d (Opht.c Art.san; 20 eyes) or ciliary sulc us implant.d phakic IOL (St.ar ICL; 16 eyes). t.e dist.nce bet.een t.e phakic I OL and t.e cryst.lline lens and t.e cornea as well as rot.t.on around t.e opt.ca l axis was evaluat.d using Scheimpflug phot.graphy at.1, 3 t. 6, and 12 mont.s p ost.urgery. t.e ant.rior chamber phakic IOLs showed no significant.movement.in a nt.ropost.rior direct.on. t.e post.rior chamber phakic IOL showed a significant.movement.t.ward t.e cryst.lline lens bet.een post.perat.ve mont.s 3 and 12. t.e median amount.of rot.t.on around t.e opt.cal axis bet.een t.e 3 and t.e 12 mon t. evaluat.on was 1.9 degrees (range= 0.0-33.5 degrees) for t.e NuVit., 0.6 deg rees (range=0.0 3.5 degrees) for t.e Art.san, and 0.9 degrees (range=0.2-2.3 d egrees) for t.e ICL. Four NuVit. IOLs rot.t.d more t.an 10 degrees. t.e angle s upport.d ant.rior chamber phakic IOLs showed a generally st.ble posit.on regardi ng dist.nce t. cornea and nat.ral lens, but.rot.t.on was observed in four IOLs. t.e iris fixat.d phakic IOL showed t.e highest.overall st.bilit.. t.e post.rior chamber phakic IOL was st.ble in t.rms of rot.t.on but.had a t.ndency t. decrea se in dist.nce t.ward t.e cryst.lline lens. Int.aocular lenses implant.d in phak ic eyes followed for 12 mont.s demonst.at. st.ble IOL posit.on overall.

关键词：睫状沟植入有晶状体眼 Scheimpflug 人工晶体房角近视性随机临床研究倾斜程度

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采用Zyopt.x3.1系统的波前导LASIK术对近视及复合近视性散光矫正的1年随访研究:临床预后及高阶像差的改变

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世界核心医学期刊文摘（眼科学分册） 2005年第3期1卷 60-60页

作者： kohnen t. Bühren J. Kühne C. Mirshahi A. 张少娟

t. assess safet., efficacy, predict.bilit., st.bilit., and change in aberrat.o ns aft.r wavefront.guided LASIK for myopia and myopic ast.gmat.sm. Prospect.ve, nonrandomized, self-cont.olled t.ial. Wavefront.guided LASIK was performed in 97 eyes in a 1-year t.ial. t.eat.d eyes had a mean subject.ve manifest.spheric al equivalent.(SE) of -5.22±2.07 diopt.rs (D), wit. a range of -0.25 t. -9.0 0 D of myopia and 0 t. -3.25 D of ast.gmat.sm. Aft.r a microkerat.me cut. a wav efront.based excimer ablat.on (Zyopt.x 3.1) was performed. t.e full t.eat.ent.t.o achieve emmet.opia of an early nomogram provided by t.e syst.m manufact.rer wa s used in all procedures. Safet., efficacy, predict.bilit., and st.bilit. were e valuat.d at.1, 3, and 12 mont.s post.perat.vely. Wavefront.changes of higher ord er aberrat.ons (HOAs) at.1 year were det.rmined for pupil sizes of 3.5 and 6 mm. At.1 year post.perat.vely, uncorrect.d visual acuit. (VA) was 20/20 or bet.er i n 83%of t.e eyes, and 20/40 or bet.er in 98%. t.e mean subject.ve manifest.SE at.1 year was -0.25±0.43 D; it.was wit.in 0.50 D in 77%and wit.in 1.0 D in 95 %. No eye lost.t.spect.cle correct.d VA (BSCVA) at.1 year post.operat.vely; 40 eyes gained 1 line of BSCVA, and 5 eyes gained 2 lines. t.e t.t. l HOA root.meansquare (RMS) increased on average by a fact.r of 1.23±0.57 wit. a 3.5-mm pupil; for t.e 6 mm pupil, t.e increase fact.r was 1.52±0.36. No chan ge or reduct.on in t.e t.t.l HOA RMS was observed in 45.5%of t.e eyes for a 3.5 -mm pupil and in 20.6%for a 6-mm pupil. t.ere was a significant.increase of p rimary spherical aberrat.on (Z 4,0) by a fact.r of 4.11±10.17 for 3.5-mm pupil s and 4.31±6.76 for 6-mm pupils. Wavefront.guided LASIK using Zyopt.x 3.1 is an effect.ve and safe procedure for t.e t.eat.ent.of myopia and myopic ast.gmat. sm. Alt.ough in close t. half of t.e eyes HOAs could be reduced, t.ere was st.ll undercorrect.on and induct.on of HOAs wit. t.e algorit.m employed.

关键词：近视性散光 LASIK术 Zyopt.x3.1 高阶像差随访研究未矫正视力瞳孔大小波前手术系统正视眼

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Profiling of circular RNA N^(6)-met.yladenosine in moso bamboo(Phyllost.chys edulis)using nanopore-based direct.RNA sequencing

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Journal of Int.grat.ve Plant.Biology 2020年第12期62卷 1823-1838页

作者： Yongsheng Wang Huihui Wang Feihu Xi Huiyuan Wang Ximei Han Went.o Wei Hangxiao Zhang Qianyue Zhang Yushan Zheng Qiang Zhu Markus V.kohnen Anireddy S.N.Reddy Lianfeng Gu Basic Forest.y and Prot.omics Research Cent.r College of life scienceFujian Agriculture and Forestry UniversityFuzhou 350002China Basic Forest.y and Prot.omics Research Cent.r College of ForestryFujian Agriculture and Forestry UniversityFuzhou 350002China College of Forest.y Fujian Agriculture and Forestry UniversityFuzhou 350002China Depart.ent.of Biology and Program in Cell and Molecular Biology Colorado State UniversityFort CollinsColoradoUSA

N6-met.yladenosine(m^(6)A)is a prevalent.modificat.on in messenger RNAs and circular RNAs t.at.play import.nt.roles in regulat.ng various aspect. of RNA ***,t.e occurrence of t.e m^(6)A modificat.on in plant.circular RNAs has not.been report.d.A widely used met.od t. ident.fy m^(6)A modificat.ons relies on m^(6)A-specific ant.bodies followed by next.generat.on sequencing of precipit.t.d RNAs(MeRIP-Seq).However,one limit.t.on of MeRIP-Seq is t.at.it.does not.provide t.e precise locat.on of m^(6)A at.single-nucleot.de *** more recent.sequencing t.chniques such as Nanopore-based direct.RNA sequencing(DRS)can overcome such limit.t.ons,t.e t.chnology does not.allow sequencing of circular RNAs,as t.ese molecules lack a poly(A)***,we developed a novel met.od t. det.ct.t.e precise locat.on of m^(6)A modificat.ons in circular RNAs using Nanopore *** first.enriched our samples for circular RNAs,which we t.en fragment.d and sequenced on t.e Nanopore plat.orm wit. a cust.mized *** t.is met.od,we ident.fied 470 unique circular RNAs from DRS reads based on t.e back-spliced junct.on *** exonic circular RNAs,about.10%cont.ined m^(6)A sit.s,which mainly occurred around accept.r and donor splice *** st.dy demonst.at.s t.e ut.lit. of our ant.body-independent.met.od in ident.fying t.t.l and met.ylat.d circular RNAs using Nanopore *** met.od has t.e addit.onal advant.ge of providing t.e exact.locat.on of m^(6)A sit.s at.single-base resolut.on in circular RNAs or linear t.anscript. from non-coding RNA wit.out.poly(A)t.ils.

关键词： circular precise overcome

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激光原位角膜磨镶术后玻璃体后脱离的发病率研究

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世界核心医学期刊文摘（眼科学分册） 2006年第8期2卷 36-37页

作者： Mirshahi A. Schpfer D. Gerhardt.D. t. kohnen 刘瑛 Depart.ent.of Opht.almology Johann Wolfgang Goethe UniversityTheodor-Stern-Kai 760590 Frankfurt amMain Germany

Background: Vit.eoret.nal complicat.ons are rare in laser in sit. kerat.mileusis (LASIK). Increase in int.aocular pressure caused by int.aoperat.ve suct.on wit. subsequent.deforming of t.e ocular globe and excimer laser shock during t.e ablat.on have been discussed as possible causes. t.e purpose of t.is st.dy was t. det.rmine t.e effect.of LASIK on t.e vit.eous body. Pat.ent. and met.ods: In a prospect.ve st.dy we performed ocular ult.asonography (B scan) immediat.ly before and 1 week aft.r LASIK procedure in 103 myopic or myopic ast.gmat.c eyes (53 pat.ent., mean age 36.3 years, 32 women, 21 men). In part.cular, t.e prevalence, localizat.on, and ext.nt.of post.rior vit.eous det.chment.(PVD) were det.rmined. Result.: t.e mean spherical equivalent.was- 4.85 D (range- 1.25 t.- 8.38) and t.e mean ant.ropost.rior ocular globe lengt. was 25.13 mm (range 23.31- 27.65). Ninet.-five eyes (92.2% ) had no PVD preoperat.vely. Nine eyes out.of t.is group (seven pat.ent., 9.5% ) developed incomplet. PVD as assessed 1 week post.perat.vely. Eight.eyes (7.8% ) had a part.al PVD preoperat.vely and in only one eye was an ext.nsion of vit.eous det.chment.observed aft.r t.e surgery. None of t.e preoperat.vely measured paramet.rs could predict.t.e occurrence of PVD by LASIK. Conclusions: LASIK may in rare cases lead t. new occurrence of PVD or ext.nsion of a previously exist.ng part.al PVD.

关键词：玻璃体后脱离近视散光准分子激光玻璃体视网膜眼压增高前后径不完全性

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Mult.-omics of Circular RNAs and t.eir Responses t. Hormones in Moso Bamboo(Phyllost.chys edulis)

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Genomics, Prot.omics & Bioinformat.cs 2023年第4期21卷 866-885页

作者： Yongsheng Wang Huihui Wang Huiyuan Wang Ruifan Zhou Ji Wu Zekun Zhang Yandong Jin t.o Li Markus V.kohnen Xuqing Liu Went.o Wei Kai Chen Yubang Gao Jiazhi Ding Hangxiao Zhang Bo Liu Chent.o Lin Lianfeng Gu Basic Forest.y and Prot.omics Research Cent.r College of ForestryFujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyFujian Agriculture and Forestry UniversityFuzhou 350002China College of Forest.y Fujian Agriculture and Forestry UniversityFuzhou 350002China Depart.ent.of Molecular Cell and Developmental BiologyUniversity of California at Los AngelesLos AngelesCA 90095USA

Circular RNAs(circRNAs)are endogenous non-coding RNAs wit. covalent.y closed st.uct.res,which have import.nt.funct.ons in ***,t.eir biogenesis,degradat.on,and funct.on upon t.eat.ent.wit. gibberellins(GAs)and auxins(1-napht.aleneacet.c acid,NAA)remain ***,we syst.mat.cally ident.fied and charact.rized t.e expression pat.erns,evolut.onary conservat.on,genomic feat.res,and int.rnal st.uct.res of circRNAs using RNase R-t.eat.d libraries from moso bamboo(Phyllost.chys edulis)***,we invest.gat.d t.e biogenesis of circRNAs dependent.on bot. cis-and *** explored t.e funct.on of circRNAs,including t.eir roles in regulat.ng microRNA(miRNA)-relat.d genes and modulat.ng t.e alt.rnat.ve splicing of t.eir linear ***,we developed a cust.mized degradome sequencing approach t. det.ct.miRNA-mediat.d cleavage of ***,we present.d a comprehensive view of t.e part.cipat.on of circRNAs in t.e regulat.on of hormone met.bolism upon t.eat.ent.of bamboo seedlings wit. GA and ***,our st.dy provides insight. int. t.e biogenesis,funct.on,and miRNA-mediat.d degradat.on of circRNAs in moso bamboo.

关键词： Alt.rnat.ve splicing Circular RNA Degradome Phyllost.chys edulis Phyt.hormone

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Effect.of honey on oxidat.on, chlorinat.on and nit.at.on by purified equine myeloperoxidase

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Journal of Coast.l Life Medicine 2017年第9期5卷 398-402页

作者： Saad Aissat.Hama Benbarek t.ierry Franck St.phan kohnen Didier Sert.yn Moussa Ahmed Ange Mouit.ys-Mickalad Inst.t.t. of Vet.rinary Sciences Ibn Khaldoun UniversityTiaret14000Algeria Pharmacognosy and Api-Phyt.t.erapy Research Laborat.ry Mostaganem UniversityMostaganemAlgeria Facult. of Sciences of Nat.re and Life Mascara UniversityMascara29000Algeria Cent.e for Oxygen Research and Development(CORD)Institute of Chemistry B6aUniversity of LiègeSart Tilman4000Liège1Belgium Depart.ent.of Clinical Sciences Large Animal SurgeryFaculty of Veterinary MedicineB41University of LiègeSart Tilman4000Liège1Belgium Celabor Centre de Services Scientifique et Technique aux EntreprisesZoning Industriel de Petit-RechainAvenue du Parc384650HerveBelgium

Object.ve: t. evaluat. t.e ant.oxidant.effect.of honey using t.o classical met.ods generally used, and for t.e first.t.me t. t.st.t.e effect.of honey on t.e oxidat.on, chlorinat.on and nit.at.on by purified equine myeloperoxidase (MPO). Met.ods: t.e ant.oxidant.act.vit. of t.ree Algerian honey samples (nect.r honey, mixed honey and honeydew honey) was evaluat.d by t.o classical met.ods, t.e ferric- reducing/ant.oxidant.power (FRAP) assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging capacit.. Result.: Honeydew honey had t.e highest.reducing power and DPPH radical-scavenging act.vit., whereas nect.r honey showed t.e lowest.reducing power and DPPH radical-scavenging act.vit.. All honey samples showed a significant.inhibit.ry effect.on t.e chlorinat.on act.vit. of equine MPO, but.honeydew honey was t.e weakest.inhibit.r. t.e t.ree samples were poorly inhibit.r on t.e MPO oxidat.on and nit.at.on act.vit.es, except.for nect.r honey t.at.exert.d an inhibit.ry effect.at.t.e highest.t.st.d concent.at.on of 10%. t.ese lat.r result. seem t. cont.adict.t.ose obt.ined wit. DPPH and FRAP. Conclusions: t.e ant.oxidant.capacit. of honey is mainly due t. t.e phenolic compounds and flavonoids it.cont.ined. It.has been suggest.d t.at.MPO might.be involved in t.e ant.oxidant. not.pro-oxidant. act.vit. of phenolic compounds.

关键词： Honey Myeloperoxidase Chlorinat.on Oxidat.on Nit.at.on

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镜中的奇遇

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现代装饰（家居） 2011年第11期 96-103页

作者： Oskar kohnen Fabian Freyt.g Gerrit.Engel 代照 Lecarolimit.d GbR.

项目地址德国柏林项目面积400m^2该公寓位于一栋大楼的顶层,大楼位于一个大型文化艺术区中,所处的街道狭窄,交通颇为拥挤,且与一个足球场毗邻。在改造前,公寓内部的空间分隔比较混乱,设计师在新方案中采用了单一重复的单元,以求达到空... 详细信息

项目地址德国柏林项目面积400m^2该公寓位于一栋大楼的顶层,大楼位于一个大型文化艺术区中,所处的街道狭窄,交通颇为拥挤,且与一个足球场毗邻。在改造前,公寓内部的空间分隔比较混乱,设计师在新方案中采用了单一重复的单元,以求达到空间上的呼应性和连续性。从休息区到吧台到厨房,以及墙壁,整个空间分布展现出一种新的逻辑性。基于其位处顶层的优势,设计师在材料的选择和空间的安排上,都突出了在城市上空俯瞰城市风光的精巧构思。

关键词：城市风光项目地址项目面积文化艺术休息区主卧区中手工切割橘黄色感官享受

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金丝桃提取物WS5570(圣约翰草)治疗急性期中重度抑郁:与帕罗西汀比较的随机对照双盲非劣效性试验

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英国医学杂志中文版 2005年第3期8卷 194-195页

作者： A Szegedi R kohnen A Dienel M Kieser 朱丽琳

目的：以帕罗西汀为对照，观察金丝桃提取物WS 5570(圣约翰草)在治疗中重度抑郁症患者中的作用。设计：随机、双盲、双模拟、多中心对照非劣效性试验。地点：德国的21家初级保健心理诊所。研究对象：Hamilt.n抑郁量表(包括17个测试项)... 详细信息

目的：以帕罗西汀为对照，观察金丝桃提取物WS 5570(圣约翰草)在治疗中重度抑郁症患者中的作用。设计：随机、双盲、双模拟、多中心对照非劣效性试验。地点：德国的21家初级保健心理诊所。研究对象：Hamilt.n抑郁量表(包括17个测试项)测量总评分≥22的251名门诊急性抑郁症患者。干预：患者每日分3次服用900 mg金丝桃提取物WS 5570，或每日一次性服用20 mg帕罗西汀，共6周。初期治疗无效的患者，2周后将每日服药量分别增加至1800 mg WS 5570或40 mg帕罗西汀。主要疗效测评：治疗前和治疗42天后Hamilt.n抑郁量表评分的变化(主要结果)。其次为Mont.omery-Åsberg抑郁评价量表、临床疗效总评量表和Beck抑郁量表的评分变化。结果：在WS 5570组，Hamilt.n抑郁量表总评分较治疗前平均下降了14.4点(标准差SD 8.8)，相当于治疗前评分的56.6%(SD 34.3%)；在帕罗西汀组，平均下降了11.4点(标准差8.6)，相当于治疗前的44.8%(SD 33.5%)(意向治疗分析；有效病例分析也得出了类似的结果)。意向治疗分析(WS 5570与帕罗西汀的97.5%单侧可信下限的差值为1.5点)和有效病例分析(可信下限差值为0.7点)表明，WS 5570的非劣效性和统计学优效性高于帕罗西汀。两种情况的低限值都超过预先设定的非劣效边界(-2.5)和优效边界(0)。WS 5570和帕罗西汀的不良反应发生率分别为每日0.035和0.060。结论：在中重度抑郁症患者的治疗中，金丝桃提取物WS 5570与帕罗西汀疗效相当或更优于帕罗西汀，并且耐受性更好。

关键词：金丝桃提取物帕罗西汀圣约翰草非劣效性随机对照重度抑郁治疗急性期试验双盲抑郁症患者中重度

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