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Chromosome-level genome assembly of the diploid blueberry Vaccinium darrowii provides insights into its subtropical adaptation and cuticle synthesis

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Plant Communications 2022年第4期3卷 105-123页

作者： Fuqiang Cui Xiaoxue Ye Xiaoxiao Li Yifan Yang Zhubing Hu kirk Overmyer Mikael Brosche Hong Yu Jarkko Salojarvi College of Forestry and Biotechnology State Key Laboratory of Subtropical SilvicultureZhejiang A&F UniversityLin’anHangzhou 311300China Key Laboratory of Biology and Genetic Resources of Tropical Crops Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikouHainan 571101China School of Biological Sciences Nanyang Technological UniversitySingaporeSingapore Organismal and Evolutionary Biology Research Program Faculty of Biological and Environmental Sciencesand the Viikki Plant Science CentreUniversity of HelsinkiPO Box 65(Viikinkaari 1)00014 HelsinkiFinland Institute of Botany Jiangsu Province and Chinese Academy of SciencesNanjingChina State Key Laboratory of Cotton Biology Department of BiologyInstitute of Plant Stress BiologyHenan UniversityKaifengChina

Vaccinium darrowii is a subtropical wild blueberry species that has been used to breed economically important southern highbush *** adaptive traits of *** to subtropical climates can provide valuable information for breeding blueberry and perhaps other plants,especially against the background of global ***,we assembled the *** genome into 12 pseudochromosomes using Oxford Nanopore long reads complemented with Hi-C scaffolding technologies,and we predicted 41815 genes using RNA-sequencing *** analysis across three Vaccinium species revealed a highly conserved genome structure,with the highest collinearity between *** and Vaccinium *** conserved genome structure may explain the high fertility observed during crossbreeding of *** with other blueberry *** of gene expansion and tandem duplication indicated possible roles for defense-and flowering-associated genes in the adaptation of *** to the *** SOC1 genes in *** were identified based on phylogeny and expression *** are covered in a thick cuticle layer and contain anthocyanins,which confer their powdery blue *** RNA sequencing,we delineated the cuticle biosynthesis pathways of Vaccinium species in *** result can serve as a reference for breeding berries whose colors are appealing to *** *** reference genome,together with the unique traits of this species,including its diploid genome,short vegetative phase,and high compatibility in hybridization with other blueberries,make *** a potential research model for blueberry species.

关键词： Vaccinium darrowii blueberry genome sequence cuticle subtropical adaptation

Mission impossible completed:unlocking the nomenclature of the largest and most complicated subgenus of Cortinarius,Telamonia

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Fungal Diversity 2020年第5期104卷 291-331页

作者： Kare Liimatainen Tuula Niskanen Bálint Dima Joseph F.Ammirati Paul M.kirk Ilkka Kytövuori Jodrell Laboratory Royal Botanic GardensKewSurrey TW93ABUK Botanical Museum University of HelsinkiP.O.Box 700014 HelsinkiFinland Department of Plant Anatomy Institute of BiologyEötvös Loránd UniversityPázmány Péter sétány 1/C1117 BudapestHungary Department of Biology University of WashingtonBox 351800SeattleWA 98195-1800USA

So far approximately 144,000 species of fungi have been named but sequences of the majority of them do not exist in the public ***,the quality and coverage of public barcode databases is a bottleneck that hinders the study of *** is the largest genus of Agaricales with thousands of species *** most diverse subgenus in Cortinarius is Telamonia and its species have been considered one of the most taxonomically challenging in the *** high diversity combined with convergent,similar appearing taxa have earned it a reputation of being an impossible group to *** this study a total of 746 specimens,including 482 type specimens representing 184 species were ***,a significant number of old types were successfully sequenced,105 type specimens were over 50 years old and 18 type specimens over 100 years ***,20 epi-or neotypes are proposed for recently commonly used older *** study doubles the number of reliable DNA-barcodes of species of *** Telamonia in the public sequence *** is also the first extensive phylogenetic study of the subgenus.A majority of the sections and species are shown in a phylogenetic context for the first *** study shows that nomenclatural problems,even in difficult groups like *** Telamonia,can be solved and consequently identification of species based on ITS barcodes becomes an easy task even for non-experts of the genus.

关键词： ITS Type study Barcode Neotype Phylogeny Section

迷宫密封结构对泄漏量和轴系临界转速影响分析研究

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振动工程学报 2013年第6期26卷 823-830页

作者：马文生陈照波焦映厚 R G kirk 中航工业沈阳发动机设计研究所辽宁沈阳110015 哈尔滨工业大学机电工程学院黑龙江哈尔滨150001 弗吉尼亚理工大学转子动力学实验室美国24061

运用数值方法对转子-轴承-密封系统动力学模型求解,对3种密封间隙、8种密封直径、8种压差、8种入口损失率和21种密封长度对泄漏量和临界转速的影响进行研究;通过密封结构对轴系临界转速影响规律进行研究,对比分析了有、无密封力作用下转... 详细信息

运用数值方法对转子-轴承-密封系统动力学模型求解,对3种密封间隙、8种密封直径、8种压差、8种入口损失率和21种密封长度对泄漏量和临界转速的影响进行研究;通过密封结构对轴系临界转速影响规律进行研究,对比分析了有、无密封力作用下转子-轴承-密封系统对临界转速影响。研究结果表明:通过与DYNLAB程序、TASCFlow程序的结果对比分析,该数学模型能较好的模拟计算泄漏量和转子系统临界转速;通过对泄漏量影响规律研究,得出泄漏量随着密封间隙、密封直径和密封长度做增大而增大,泄漏量随压差和入口损失率的增大而减小。通过对临界转速影响规律研究,得到考虑密封会提高临界转速,密封长度的变化对临界转速的影响最大、密封间隙的变化对临界转速影响最小。

关键词：迷宫密封动力学模型转子-轴承-密封系统泄漏量临界转速

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免疫的代价(英文)

动物学报 2004年第6期50卷 961-969页

作者： kirk C. KLASING Department of Animal Science University of California Davis California 95616 USA

免疫系统发育的代价主要是为B淋巴细胞和T淋巴细胞发育抗原识别多样性时的低效率过程提供能量 ,为开始形成白血球提供基质 (如氨基酸和脂肪 ) ,并为胚胎和雏鸟提供白细胞。维持免疫系统的代价与持续产生白细胞、免疫球蛋白以及其它血浆... 详细信息

免疫系统发育的代价主要是为B淋巴细胞和T淋巴细胞发育抗原识别多样性时的低效率过程提供能量 ,为开始形成白血球提供基质 (如氨基酸和脂肪 ) ,并为胚胎和雏鸟提供白细胞。维持免疫系统的代价与持续产生白细胞、免疫球蛋白以及其它血浆蛋白时的营养分配有关 ,这些血浆蛋白用于取代在正常的细胞代谢中丢失的蛋白质以及细胞间的蛋白质。利用免疫系统阻碍可能的病源体入侵的代价有两种主要形式。第一 ,由于白细胞参与效应子机制时发生的损害、组织整合性以及寄主细胞的存活力的损伤 ,组织功能有一些损失。第二 ,在活化相应类型的细胞并产生其效应子作用时存在着营养耗费。病源体挑战所带来的主要代价是系统的急性期反应(特别是肝脏的复原 )

关键词：免疫系统白细胞血浆蛋白急性期反应抗原识别正常 B淋巴细胞营养分配病源体基质

偏心率和转速对迷宫密封力和动力学参数影响分析研究

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振动工程学报 2012年第3期25卷 282-288页

作者：马文生陈照波焦映厚 R G kirk 哈尔滨工业大学机电工程学院黑龙江哈尔滨150001 弗吉尼亚理工大学转子动力学实验室美国24061

通过数值方法和商用CFD(计算流体动力学)软件对密封-转子系统进行建模和求解,研究了5种偏心率和5种转速下的计算机求解时间、流场压力分布、密封力的变化情况,并对泄漏量影响分析和迷宫密封动力学参数影响进行分析研究。研究结果表明:... 详细信息

通过数值方法和商用CFD(计算流体动力学)软件对密封-转子系统进行建模和求解,研究了5种偏心率和5种转速下的计算机求解时间、流场压力分布、密封力的变化情况,并对泄漏量影响分析和迷宫密封动力学参数影响进行分析研究。研究结果表明:该方法能较好地模拟计算迷宫密封泄漏量和动力学参数,得到腔室压力随着偏心率增大而增大,密封切向力随着偏心率、转速的增大而增大,密封径向力随着偏心率、转速的增大而负向增大;通过密封长度、密封间隙、密封压差对泄漏量影响计算,三者变化率分别为6.62%,65.21%和69.97%,表明密封间隙和压差变化是影响泄漏量变化的重要影响因素;通过密封压差和密封长度对动力学参数影响分析,得出增大密封压差和密封长度不利于系统稳定、增大密封间隙会使得系统趋于稳定。

关键词：迷宫密封偏心率转速泄漏量动力学参数

The Wedding Video Handbook： How to Succeed in the Wedding Video Business (DV Expert Series)

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2005年

作者： kirk Barber.

ISBN: (纸本)9781578202812

在线阅读本书 For readers who want to become part of the thriving wedding video industry, Wedding Video Handbook explains everything a wedding videographer needs to know. Each chapter explores a different part of either the business or the production ends, and covers topics including secrets for getting clients, selecting the proper equipment, and tips on capturing special wedding moments despite difficult filming conditions. The book includes handy primers on marketing and advertising, handling phone calls and appointments, pre-production preparations, what to shoot on the wedding day, interacting with other vendors, editing and packaging the DVD or video, and generating referrals. Fully up-to-date with information on the newest tools and equipment used in this rapidly-evolving market as well as the cutting-edge trends in wedding video products, Wedding Video Handbook is packed with practical advice from a pro who has spent years in the field. Fully up-to-date with information on the newest tools and equipment used in this rapidly-evolving market as well as the cutting-edge trends in wedding video products, The Wedding Video Handbook is packed with practical advice from a pro who's spent years in the field.

关键词： Video recordingsProduction and direction.Wedding photographyVocational guidance.

来源：畅想之星图书光盘版（南... 评论

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转速数对滑动轴承动力学系数影响研究

振动与冲击 2014年第5期33卷 8-13页

作者：马文生陈照波焦映厚 kirk R G 中航工业沈阳发动机设计研究所沈阳110015 哈尔滨工业大学机电工程学院哈尔滨150001 弗吉尼亚理工大学转子动力学实验室美国24061

建立了滑动轴承的基于短轴承非线性油膜力模型,通过转速对轴承动力学特性的影响研究,得到了偏心率、最小油膜厚度、最大油膜压力、摩擦功耗、温升、临界轴颈质量、刚度系数、阻尼系数、二维和三维油膜压力的影响因素研究;在对三维油膜... 详细信息

建立了滑动轴承的基于短轴承非线性油膜力模型,通过转速对轴承动力学特性的影响研究,得到了偏心率、最小油膜厚度、最大油膜压力、摩擦功耗、温升、临界轴颈质量、刚度系数、阻尼系数、二维和三维油膜压力的影响因素研究;在对三维油膜压力分析时发现转速存在一个临界值,当转速低于这个临界值时,转速对最大油膜压力影响较大,当转速高于这个临界值时,转速对最大油膜压力影响不大。

关键词：转速数动力学系数 Reynolds方程 dynamic rotor-bearing system (DyRoBes)

Psychological effects of colorectal cancer screening: Participants vs individuals not invited

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World Journal of Gastroenterology 2016年第43期22卷 9631-9641页

作者： Benedicte kirk?en Paula Berstad Edoardo Botteri Linn Bernklev Badboni El-Safadi Geir Hoff Thomas de Lange Tomm Bernklev Department of bowel cancer screening Cancer Registry of Norway Institute of Clinical Medicine University of Oslo Department of Internal Medicine Vestre Viken Hospital Trust B?rum Hospital Department of Gastroenterology ?stfold Hospital Trust Department of Research and Development Telemark Hospital Trust Department of Health Management and Health Economics Institute of Health and Society University of Oslo

AIM To investigate the possible long-term psychological harm of participating in colorectal cancer(CRC)screening in *** In a prospective, randomized trial, 14294 participants(aged 50-74 years) were invited to either flexible sigmoidoscopy(FS) screening, or a faecal immunochemical test(FIT)(1:1). In total, 4422 screening participants(32%) completed the questionnaire, which consisted of the Hospital Anxiety and Depression Scale and the SF-12, a generic health-related quality of life(HRQOL) measurement, when invited to screening and one year after the invitation. A control group of 7650 individuals was invited to complete the questionnaire only, at baseline and one year after, and 1911(25%) completed the *** Receiving a positive or negative screening result and participating in the two different screening modalities did not cause clinically relevant mean changes in anxiety, depression or HRQOL after one year. FS screening, but not FIT, was associated with an increased probability of being an anxiety case(score ≥ 8) at the one-year follow-up(5.6% of FS participants transitioned from being not anxious to anxious, while 3.0% experienced the reverse). This increase was moderately significantly different from the changes in the control group(in which the corresponding numbers were 4.8% and 4.5%, respectively), P = 0.06. CONCLUSION Most individuals do not experience psychological effects of CRC screening participation after one year, while FS participation is associated with increased anxiety for a smaller group.

关键词： Colorectal cancer screening Flexible sigmoidoscopy Fecal immunochemical test Anxiety Health-related quality of life Control group

An assessment of African lion Panthera leo sociality via social network analysis： prerelease monitoring for an ex situ reintroduction program

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Current Zoology 2017年第3期63卷 301-311页

作者： Emma J. DUNSTON Jackie ABEL Rebecca E. DOYLE Jacqui kirk Victoria B. HILLEY Andrew FORSYTH Emma JENKINS Rafael FREIRE School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga NSW 2678 Australia Social and Conservation Psycology Coventry University Coventry CV1 5FB UK African Lion & Environmental Research Trust Melrose Farm Livingstone 23345 Zambia Animal Welfare Science Centre The University of Melbourne Parkville Victoria 3010 Australia African Lion & Environmental Research Trust Antelope Park Gweru Midlands Zimbabwe Siyafunda The Greater Makalali Private Game Reserve Harmony 27 Hoedspruit 0895 South Africa

The wild population of the African lion Panthera leo continues to decline, requiring alternate conservation programs to be considered. One such program is ex situ reintroduction. Prior to release, longterm monitoring and assessment of behavior is required to determine whether prides and coalitions behave naturally and are sufficiently adapted to a wild environment. Social network analysis （SNA） can be used to provide insight into how the pride as a whole and individuals within it, function. Our study was conducted upon 2 captive-origin prides who are part of an ex situ reintroduction program, and 1 wild pride of African lion. Social interactions were collected at all occurrence for each pride and categorized into greet, social grooming, play, and aggression. Betweenness centrality showed that offspring in each pride were central to the play network, whereas degree indicated that adults received （indegree） the greatest number of overall social interactions, and the adult males of each pride were least likely to initiate （outdegree） any interactions. Through the assessment of individual centrality and degree values, a social keystone adult female was identified for each pride. Social network results indicated that the 2 captive-origin prides had formed cohesive social units and possessed relationships and behaviors comparable with the wild pride for the studied behaviors. This study provided the first SNA comparison between captive-bred origin and a wild pride of lions, providing valuable information on individual and pride sociality, critical for determining the success of prides within an ex situ reintroduction program.

关键词： African lion Panthera leo conservation ex situ reintroduction social interactions.