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Genotoxic and Histopathological Effects of Water Pollution on <i>Clarias gariepinus</i>Fish at Fayoum Governorate, Egypt

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Natural Resources 2020年第11期11卷 499-519页

作者： Khalid H. Zaghloul Heba A. mohamed Abdulkareem M. Abdullatef mohamed W. khalil Department of Zoology Faculty of Science Fayoum University Fayoum Egypt

Increasing land reclamation in El-Fayoum governorate has led to increase the amount of drainage water that discharged without prior treatments to two main drains, El-Wadi and El-Bats drainages loaded by salts and heavy metals. So, the present investigation aims to evaluate quality of water samples collected from a branch of the river Nile (El-Lahon canal) and the main drains (El-Bats and El-Wadi) at Fayoum governorate on some biomarkers of the Nile catfish;Clarias gariepinus inhabiting the studied degradable aquatic habitats. Water and nighty fish samples colleted from the different studied sites of collection by the help of fishermen for physicochemical analyes of water and fish biochemical analyses and histopathological alteration. Results revealed highly significant differences at P ≤ 0.01 with the highest water ammonia, nitrite, heavy metals (Cu, Zn, Pb and Cd) and lowest dissolved oxygen content accompanied by metals bioaccumulation in vital organs of fish collected from the main drains (El-Bats and El-Wadi) in comparision with that collected from the studied branch of the river Nile. Higher bioaccumulation of the studied heavy metals in gills, liver, kidney and muscles of Clarias gariepinus collected from the main drains significant increase in serum glucose, liver and kidney functions, disturbance in protein profile of fish collected from the studied main drains than that of fish collected from the river Nile branch, El-Lahon that showed more or less normal values. Moreover, histopathological alterations in gills, liver, kidneys and clear DNA strand breaks in fish liver cells increased statistically in Clarias gariepinus col

关键词： Drainage Water Catfish Genotoxicity Histopathology Biochemical Changes

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Inter-Observer Reliability of Fused Time-of-Flight MR Angiography and 3D Steady State Sequence versus 3D Contrast Enhanced Images in Evaluation of Neurovascular Compression

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Open Journal of Modern Neurosurgery 2022年第2期12卷 86-96页

作者： mohamed Mahmoud Elsherbini Ali Hassan Elmokadem Ahmed El-Morsy Fatma mohamed Sherif Amr Farid khalil Department of Neurosurgery Mansoura University Mansoura Egypt Department of Radiology Mansoura University Mansoura Egypt Department of Radiology Farwaniya Hospital Kuwait City Kuwait

Purpose: Is to evaluate the accuracy of fused 3D time-of-flight (TOF) MR angiography and 3D Steady-State sequence (FIESTA) versus 3D contrast-enhanced T1 weighted images in evaluation of neurovascular compression via an inter-observer agreement protocol. Methods: Patients presented with trigeminal neuralgia, tinnitus, or facial hemispasm were examined using 3D-TOF-MRA, 3D-FIESTA, and 3D contrast-enhanced T1WI of the cerebellopontine angle to assess neurovascular compression. Two independent readers assessed the location, signal alteration, offending vascular structure, and grade of neurovascular compression using fused 3D-TOF-MRA and 3D-FIESTA versus contrast-enhanced T1 weighted images. The Kappa test for interobserver agreement was done. Results: The final study cohort consisted of 56 patients (42 females and 14 males) with a mean age of 38.25 ± 1.94. AICA was the offending vessel for 32 (57.1%) patients. The most common offending nerve was the trigeminal nerve in 26 patients, followed by facial/vestibulocochlear complex in 18 patients, and solely the 8th nerve in 12 patients. All three grades of compression were encountered in this study with percentages of 48.2% (27/56), 30.3% (17/56), and 21.4% (12/56) for grades I, II, III respectively. Fused TOF and steady-state images, and contrast-enhanced images showed perfect agreement for detection of the side of compression, the relation between nerve and vascular loop, offended neural segment, and offending vessel, while showing good agreement regarding the degree of compression. Conclusion: Fused TOF and steady-state images provide sufficient data to diagnose and grade microvascular compression syndromes comparable to contrast-enhanced images.

关键词： Neurovascular Compression Trigeminal Neuralgia Microvascular Decompression Tinnitus Vertigo Hemifacial Spasm

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Neutrophil to Lymphocyte Ratio in Acute Ischemic Stroke

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Neuroscience & Medicine 2020年第2期11卷 52-62页

作者： Menat-Allah mohamed Lashin Salma Hamed khalil Taha Kamel Alloush Sherif Anis mohamed Mahmoud Fouad Department of Neurology Faculty of Medicine Ain Shams University Cairo Egypt Department of Anesthesia Faculty of Medicine Ain Shams University Cairo Egypt

Introduction: The neutrophil to lymphocyte ratio (NLR) can be used as a marker of subclinical inflammation, and may have a predictive power in prognosis and severity of atherosclerosis-related diseases. This study aimed to assess an association between the NLR, and clinical characteristics and one-month outcome in acute ischemic stroke (AIS). Subjects and Methods: This case-control observational prospective study included 75 patients admitted to stroke unit of Ain Shams University hospitals with AIS, sub grouped into 3 equal groups according to subtype of AIS, in addition to 25 healthy individuals. The demographic characteristics of the patients, complete blood picture test results at presentation, National Institutes of Health Stroke Scale (NIHSS) scores and modified Rankin Scale (mRS) scores were recorded. The clinical outcome was assessed by the NIHSS and mRS scores after one month. Results: The total leucocyte count was significantly higher in large artery atherosclerosis (P = 0.004) and cardioembolic (P = 0.020) stroke groups, unlike lacunar stroke group (P = 0.082), when compared to controls. The neutrophils count was higher (P Conclusion: NLR was significantly higher among AIS subtypes compared to controls, but not a good predictor for one month outcome.

关键词： Neutrophil to Lymphocyte Ratio Acute Ischemic Stroke

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Effect of closed circuits drip irrigation system and lateral lines length on growth, yield, quality and water use efficiency of soybean crop

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Agricultural Sciences 2013年第2期4卷 85-90页

作者： mohamed Yousif Tayel Hani Abdel-Ghani Mansour Sabreen khalil Pibars Water Relation and Field Irrigation Department National Research Center Giza Egypt

Field experiment was conducted for one growing season (2012) in clay loam soil at the Experimental Farm of Faculty of Agriculture, Southern Illinois University at Carbondale (SIUC), USA on soybean crop to study the effect of: 1) the closed drip irrigation system: closed circuits with one and two a manifolds for lateral lines (CM1DIS;CM2DIS) and traditional drip irrigation system (TDIS) as a control;and 2) lateral lines length (LLL): LLL1, LLL2 and LLL3 (40, 60,80 m) on soybean growth, yield, oil, protein content and water use efficiency. Plants were drip irrigated every 4 days. N, K2O and P2O5 fertilizers were added via irrigation water. Data obtained could be outlined as follows: 1) According to the mean values of soybean crop growth (leaf area;plant height), yield (grain and straw), both oil and protein content and water use efficiency, the treatment used could be ranked in the following ascending orders: TDIS < CM1DIS < CM2DIS and LLL3 < LLL2 < LLL1;2) Differences in the means of the studied data among treatments used were significant at the 1% level;3) The effects of the DIC × LLL on the data obtained were significant at the 1 % level;And 4) the highest values of the obtained data and the lowest ones were achieved in the following interactions: CM2DIS × LLL1;TDIS × LLL3, respectively.

关键词： Closed Circuits Drip Irrigation Lateral Lines Soybean WUE

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Effect of drip irrigation method, nitrogen source, and flushing schedule on emitter clogging

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Agricultural Sciences 2013年第3期4卷 131-137页

作者： mohamed Yousif Tayel Sabreen khalil Pibars Hani Abdel-Ghani Mansour Water Relation and Field Irrigation Department National Research Center Giza Egypt

Field experiments were carried out at the National Research Center farm, Nubaria area, Behura Governorate, Egypt, to study the effect of nitrogen source, flushing schedule and irrigation method on emitter clogging. Peanut Giza 5 variety (Arachishy pogaea L.) was planted in sandy soil during two successive growing seasons (2010-2011) in the 1st week of May and harvested after 130 days. Treatments used are: 1) two irrigation methods: surface drip irrigation and sub-surface drip irrigation (SDI;SSDI), 2) nitrogen source (NS):NH4NO3, (NH4)2SO4 and Ca(NO3)2 (NS1, NS2 and NS3) and 3) flushing number (FL) 0, 1 and 4 (FL1, FL2;FL3). The experiments design was split-split plot and three replicates were used. Data obtained were subjected to statistical analysis. The main effects of treatments used on clogging per cent could be written the following ascending orders: SDI 3 2 1, NS1 2 3. Concerning the 1st interaction the following ascending orders denote their effects on clogging percent: SDI × FL3 2 1, SDI × NS1 2 3, SSDI × FL3 2 1, SSDI × NS1 2 3, FL1 × NS1 1 × NS2 1 × NS3, FL2 × NS1 2 × NS2 2 × NS3 and FL3 × NS1 3 × NS2 3 × NS3. The differences between any two treatments and/or any two interactions in clogging percent were significant at the 5% level. The effect of the 2nd interaction on clogging percent was significant at the 5% level. The maximum value of clogging (20.18%) and the lowest one (3.9%) were archived in the interactions: SSDI × FL1 × NS3 and SDI × FL3 × NS1, respectively.

关键词： Irrigation Method Nitrogen Source Flushing Number Water and Soil Analysis Emitter Clogging

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The combination of sitagliptin and bee honey extract potentiates the anti-proliferative properties of 5-fluorouracil on Caco-2 cell line without detectable inflammatory events

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Clinical Traditional Medicine and Pharmacology 2024年第4期5卷 65-75页

作者： Asmaa Eisa mohamed F.Elshal Shaden Muawia Hany khalil Department of Molecular Biology Genetic Engineering and Biotechnology Research InstituteUniversity of Sadat City32897Sadat CityEgypt

Background:Colon cancer originates in the colon,specifically the large *** carries a poor prognosis and high mortality rate due to late diagnosis and ***:Here our objective was to evaluate the anticancer effects of sitagliptin(Sita)in colon cancer using Caco-2 ***,we examined the role of bee honey extract in modulating cancer cell division and necrotic events commonly observed during drug ***:We monitored cell viability rate to evaluate the effect of bee honey extract compared to 5-fluorouracil(5-Fu),Sita,and their *** gain further foresights into the implicated molecular interaction,we assessed the expression outline of Raf-1 and MEK,as proliferation ***,we examined the expression outline of p53 and Caspase 3,which are associated with programmed cell death(PCD),through western blot ***:We identified the Raf-1 expression pattern as a likely target for the drug combination and bee honey extract(HE),which effectively controlled colon cancer cell *** study demonstrates that honey extract,either alone or in combination with drugs,can induce PCD by restoring the p53 and CASP-3 *** was accompanied by a synergistic effect on the production of apoptotic cytokines,particularly interlukine-6(IL-6)and IL-8,in cancer ***,the treatment modulated the levels of pro-inflammatory cytokines,including IL-1𝛼and IL-1𝛽and anti-inflammatory cytokines,including IL-4 and ***:Our findings shed light on honey extract and its combinations with 5-Fu and Sita in stimulating PCD and modulating cytokine production in Caco-2 cells.

关键词： Colon cancer Caco-2 cells 5-flourouracil Sitagliptin Bee honey Raf-1 signaling Programmed cell death Cytokines

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Influence of Sn Low Doping on the Morphological, Structural and Optical Properties of ZnO Films Deposited by Sol Gel Dip-Coating

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Advances in Materials Physics and Chemistry 2014年第5期4卷 93-104页

作者： Chewki Zegadi khalil Abdelkebir Denis Chaumont mohamed Adnane Saad Hamzaoui Laboratory of Electron Microscopy and Materials Sciences University of Science and Technology of Oran El-Mnaouer Oran Algeria Nanoform—ICB Université de Bourgogne Dijon France

In this work, Undoped Zinc Oxide (ZnO) and Sndoped Zinc Oxide (ZnO:Sn) films have been deposited by sol-gel dip coating method, where the Sn/Zn atomic ratio was 3% and 5% in the solution. The effects of Sn incorporation on morphological, structural and optical properties of ZnO films were investigated. The Scanning Electron Microscopy (SEM) showed that the morphological surface of the films was affected by Sn low doping. The X-Ray Diffraction (XRD) patterns showed that all films have polycrystalline structures, and the doping incorporation has not lead to substantial changes in the structural characteristics of ZnO films. The crystallite size was calculated using the well-known Scherrer’s formula and found to be in the range of 23 - 40 nm. The measurements from UV-Visible Spectrophotometer (U-Vis) indicated that the highest average optical transmittance in the visible region was related to Undoped ZnO film, then the optical band gap and Urbach energy values of thin films were calculated. The X-Ray Photoelectron Spectroscopy (XPS) has demonstrated that Sn is incorporated in ZnO lattice.

关键词： Sol-Gel Dip-Coating ZnO Films Sn Low Doping SEM Micrographs X-Rays Patterns Optical Properties XPS Spectrum

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Serum Bilirubin as a Predictor of Coronary Artery Disease Severity in Patients Undergoing Primary Percutaneous Coronary Intervention

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World Journal of Cardiovascular Diseases 2019年第5期9卷 309-323页

作者： Tarek Salah khalil Waleed Abdou Ibrahim mohamed Asem Ali Elmalla Department of Cardiology Faculty of Medicine Menoufia University Hospitals Menoufia Egypt El-Mahalla Cardiology Mahalla Cardiac Centre Mahalla Egypt

Introduction: Coronary artery disease (CAD) is one of the most common causes of death worldwide. In 2010, about 7 out of total 53 million deaths were due to ischemic heart disease. The aim of this study is to evaluate the relationship of serum bilirubin level with the severity and complexity of coronary artery disease (CAD) in the patients undergoing primary percutaneous coronary intervention (PCI). Materials and Methods: 70 patients with STEMI who were undergoing primary PCI were included in the study. All the patients included in the study were subjected to full routine investigations and standard coronary angiographic projections. Total bilirubin level was measured and the patients were divided into two groups. Group 1 was with serum TB (and Group 2 was with serum TB (>1 mg/dl). Severity and complexity of coronary artery lesions will be assessed using Gensini score. Results: After PCI, the two studied groups were compared regarding the number of vessels affected by one and more than one vessel disease. Single vessel disease was frequent in Group 2 (71%) with significant p value (0.003). Cardiac enzymes (troponin I) was more in Group 1 (S. Bil value (0.02). Also (ALT, AST) were more in Group 1 (p value = 0.01). By comparing the 2 groups, there was a significant difference regarding (EF) between both which was less in Group 1 (*** 1), p value significance (0, 0001). Also GENSENI was more in Group 1 (*** 1) with mean (80.35 vs 34.71) and significant pvalue (0.0001). There was a highly significant negative correlation between serum bilirubin & GENSENI score (r = -0.762, p value 0.0001). Regarding the incidence of complications, incidence was more in Group 1 (*** 1), which means a significant difference between both groups with significant p value (0.0001). There was a significant negative correlation between serum bilirubin & incidence of complications (R = -0.38, pvalue 0.001). Also, there was a significant negative correlation between GENSINI score, complication

关键词： Serum Bilirubin Coronary Artery Disease Prognosis Risk Factors

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Synthetic Approaches Towards 2-（4-oxo-4, 5-Dihyd ro-Thiazol-2-yl） Acetamide

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材料科学与工程（中英文A版） 2011年第2X期1卷 228-235页

作者： Ali khalil Shaban Ibrahim mohamed Hassan Ahmed Eyada Tarek Ahmed abdel Alaziz Gameel Ahmed mohamed mohamed El-Hag Ali Chemistry Department Faculty of Science Ain Shams University Cairo Egypt Technonlogy Engeneering Institute Tammoh Giza Egypt Chemistry Department Faculty of Science Al-Azhar University Cairo Egypt

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Detection of Potato Spindle Tuber Viroid, and Its Impact on Growth, Production of Tomatoes in Greenhouse

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Journal of Agricultural Science and Technology(B) 2012年第8期2卷 940-949页

作者： Haniyeh mohamed Algamodi Jaber Abdullah khalil Taher Ahmed Abouhliqh Khaled Ojdj College of Education Gasar bin Ghashir University of Tripoli Tripoli Libya College of Agriculture University of Tripoli Tripoli Libya College of Agriculture Ajafarh University of Tripoli Tripoli Libya College of Agriculture Ain Shams University Cairo Egypt

The viability of most tomato varieties cultivated in Libya have been tested to infect with potato spindle tuber viroid/potatoes （PSTVd） and its impact on growth and production of some of these varieties, which were mechanically inoculated with Libyan isolate of viroid PSTVd as follows： Vlkato, Sankarh, Lebda, Jasmine, Kenza and Hana. The percent of incidence were 95.95%, 90%, 90.80%, 80% and 20%, respectively. The following varieties have been contagious mechanically with viroid of PSTVd： Vlkato, zahra, Toria, Lebda, Hoda, Farwa, Alkaraz, Naziha, Rim Star and Kartika. The percent of incidence were 95.95%, 85%, 85.80%, 80%, 70.40%, 0.0%, 0.0%, respectively. The varied symptoms of wrinkle, twist, warp, swell the veins of the leaves, dark brown spots formation and a large yellow spots turned into white patches. Also the effect of the Egyptian isolate viroid PSTVd in the growth and production of varieties Jasmine, Lebda, Soberhalim, and treasure No. 185 had been studied, as the average rates of decline in the production of the fruits tomatoes/tomato 43.4% and 17% length of plants, and in the fresh weight and dry root of the sum of 35% and 37% respictively.

关键词： Potato spindle tuber viroid the infection of tomato plants nucleic acid PCR.

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