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Journal of Pharmaceutical Analysis 2017年第1期7卷 56-62页

作者： Dhiraj M. Rathod keyur R. patel Hiren N. Mistri Arvind G. Jangid Pranav S. Shrivastav Mallika Sanyal Department of Chemistry Kadi Sarva Vishwavidyalaya Gandhinagar Ahmedabad 382015 India Bioanalytical Research Department Accutest Research Lab Bodakdev Ahmedabad 380059 India Department of Chemistry School of Sciences Gujarat University Ahmedabad 380009 India Department of Chemistry St. Xavier's College Navrangpura Ahmedabad 380009 India

The present study describes a simple, reliable and reproducible liquid chromatography–tandem mass spectrometry method(LC–MS/MS) for the simultaneous determination of allopurinol and its active metabolite,oxypurinol in human plasma for a pharmacokinetic/bioequivalence study. After protein precipitation(PPT) of100 μL plasma sample with 1.0% formic acid in acetonitrile, the recovery of the analytes and allopurinol-d2 as an internal standard ranged from 85.36% to 91.20%. The analytes were separated on Hypersil Gold(150 mm×4.6 mm, 5 μm) column using 0.1% formic acid-acetonitrile(98:2, v/v) as the mobile *** was done using electrospray ionization in the positive mode. The calibration concentration range was established from 60.0 to 6000 ng/m L for allopurinol and 80.0–8000 ng/m L for oxypurinol. Matrix effect in human plasma, expressed as IS-normalized matrix factors ranged from 1.003 to 1.030 for both the analytes. The developed method was found suitable for a clinical study with 300 mg allopurinol tablet formulation in healthy subjects.

关键词： Allopurinol Oxypurinol Liquid chromatography–tandem mass spectrometry Bioequivalence study

An improved LC–MS/MS method for the quantification of alverine and para hydroxy alverine in human plasma for a bioequivalence study

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Journal of Pharmaceutical Analysis 2017年第2期7卷 95-102页

作者： Dhiraj M.Rathod keyur R.patel Hiren N.Mistri Arvind G.Jangid Pranav S.Shrivastav Mallika Sanyal Department of Chemistry Kadi Sarva VishwavidyalayaGandhinagarAhmedabad-382015India Bioanalytical Research Department Accutest Research LabBodakdevAhmedabad-380059India Department of Chemistry School of SciencesGujarat UniversityAhmedabad-380009India Department of Chemistry St.Xavier's CollegeNavrangpuraAhmedabad-380009India

A highly sensitive and selective high performance liquid chromatography–tandem mass spectrometry method was developed and validated for the quantification of alverine（ALV） and its active metabolite, para hydroxy alverine（PHA）, in human plasma. For sample preparation, solid phase extraction of analytes was performed on Phenomenex Strata-X cartridges using alverine-d5 as the internal standard. The analytes were separated on Symmetry Shield RP18（150 mm×3.9 mm, 5 μm） column with a mobile phase consisting of acetonitrile and10 mM ammonium formate（65：35, v/v）. Detection and quantitation was done by electrospray ionization mass spectrometry in the positive mode using multiple reaction monitoring. The assay method was fully validated over the concentration range of 15.0–15,000 pg/mL for ALV and 30.0–15,000 pg/mL for PHA. The intra-day and inter-day accuracy and precision（% CV） ranged from 94.00% to 96.00% and 0.48% to 4.15% for both the analytes. The mean recovery obtained for ALV and PHA was 80.59% and 81.26%, respectively. Matrix effect,expressed as IS-normalized matrix factor ranged from 0.982 to 1.009 for both the analytes. The application of the method was demonstrated for the specific analysis of ALV and PHA for a bioequivalence study in 52 healthy subjects using 120 mg ALV capsules. The assay reproducibility was also verified by reanalysis of 175 incurred subject samples.

关键词： Alverine Para hydroxy alverine Liquid chromatography–tandem mass spectrometry Bioequivalence study Human plasma

Liquid chromatography-tandem mass spectrometry method for simultaneous determination of albendazole and albendazole sulfoxide in human plasma for bioequivalence studies

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Journal of Pharmaceutical Analysis 2016年第4期6卷 226-234页

作者： Dhiraj M.Rathod keyur R.patel Hiren N.Mistri Arvind G.Jangid Pranav S.Shrivastav Mallika Sanyal Department of Chemistry Kadi Sarva Vishwavidyalaya Bioanalytical Research Department Accutest Research Lab Department of Chemistry School of SciencesGujarat University Department of Chemistry St.Xavier's College

An improved high performance liquid chromatography-tandem mass spectrometry（LC-MS/MS） method has been developed for sensitive and rapid determination of albendazole（ABZ） and its active metabolite,albendazole sulfoxide（ABZSO）,in the positive ionization *** method utilized solid phase extraction（SPE） for sample preparation of the analytes and their deuterated internal standards（ISs） from100 μL human *** chromatography was carried out on Hypurity C_（18） column using acetonitrile-2.0 mM ammonium acetate,pH 5.0（80：20,v/v） as the mobile *** assay exhibited a linear response over the concentration range of 0.200-50.0 ng/mL for ABZ and 3.00-600 ng/mL for *** recoveries of the analytes and ISs ranged from 86.03%-89.66%and 89.85%-98.94%,*** effect,expressed as IS-normalized matrix factors,ranged from 0.985 to 1.042 for the both *** method was successfully applied for two separate studies in healthy subjects using single dose of 400 mg conventional tablets and 400 mg chewable ABZ tablets,respectively.

关键词： Albendazole Albendazole sulfoxide LC-MS/MS Solid phase extraction Bioequivalence study

FibroSURE^(TM) and FibroScan~ in relation to treatment response in chronic hepatitis C virus

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World Journal of Gastroenterology 2011年第41期17卷 4581-4589页

作者： keyur patel Mireen Friedrich-Rust Yoav Lurie Mircea Grigorescu Carol Stanciu Chuan-Mo Lee Eugene R Schiff Dieter Hussinger Michael P Manns Guido Gerken Isabelle Colle Michael Torbenson Erik Pulkstenis G Mani Subramanian John G McHutchison Stefan Zeuzem Department of Gastroenterology and Hepatology Duke Clinical Research Institute Department of Gastroenterology and Hepatology J.W.Goethe University Hospital Department of Gastroenterology and Hepatology Tel Aviv Sourasky Medical Center Department of Clinical Infectious Disease Spitalul Clinic de Urgenta Department of Gastroenterology and Hepatology Institutul de Gastroenterologie si Hepatologie Department of Gastroenterology Chang Gung Memorial Hospital-Kaohsiung Medical CenterChang Gung University College of Medicine Departments of Gastroenterology and Internal Medicine University of Miami Department of Gastroenterology and Hepatology Universittsklinik Department of Gastroenterology and Hepatology Department of Gastroenterology and Hepatology Ghent University Hospital Department of Pathology Johns Hopkins University School of Medicine Department of Biostatistics Human Genome Sciences Department of Clinical Research Human Genome Sciences

AIM:To compare histological endpoint assessment using noninvasive alternatives to biopsy during treatment in a chronic hepatitis C virus(HCV)***:Patients with chronic HCV were randomized to receive interferon-based therapy for 24(genotypes 2/3)or 48(genotype 1)***～TM(FS)was assessed at baseline and at week-12 post-treatment *** biopsy for METAVIR was assessed by a single ***～ transient elastogra-phy(TE)was performed during treatment in a patient ***:Two thousand and sixty patients(n = 253 in Asia)were classif ied as METAVIR F0-1(n = 1682)or F2-4(n = 378).For F2-4,FS(n = 2055)had sensitiv-ity and specif icity of 0.87 and 0.61,respectively,with area under the receiver-operating curve of 0.82;corre-sponding values for TE(n = 214)and combined FS/TE(n = 209)were 0.77,0.88 and 0.88,and 0.93,0.68 and *** FS/TE agreement for F2-4 was 71%(κ = 0.41)and higher in Asians vs non-Asians(κ = 0.86 vs 0.35;P < 0.001).Combined FS/TE had 97% accuracy in Asians(n = 33).Baseline FS(0.38 vs 0.51,P < 0.001)and TE(8.0 kPa vs 11.9 kPa,P = 0.006)scores were lower in patients with sustained virological response than in nonresponders,and were maintained through ***:FS and TE may reliably differentiate mild from moderate-advanced disease,with a potential for high diagnostic accuracy in Asians with chronic HCV.

关键词：丙型肝炎病毒治疗慢性 TM 评估基准亚洲人工作曲线 TE

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慢性丙型肝炎病毒感染的诊断和治疗

英国医学杂志中文版 2006年第4期9卷 231-236页

作者： keyur patel Andrew J Muir John G McHutchison 张静（译）林三仁（校） Duke Clinical Research Institute Duke University Medical CenterDurham NC27707 USA 不详

在未来10～20年中，与丙肝病毒感染相关的肝病并发症将增加.需要贯彻预防策略，减少高危人群中人与人之间的传播,通过抗病毒治疗，可能维持病毒清除状态，预防疾病进展,目前的最佳治疗方法是，根据基因型和病毒学反应，应用聚乙二醇干... 详细信息

在未来10～20年中，与丙肝病毒感染相关的肝病并发症将增加.需要贯彻预防策略，减少高危人群中人与人之间的传播,通过抗病毒治疗，可能维持病毒清除状态，预防疾病进展,目前的最佳治疗方法是，根据基因型和病毒学反应，应用聚乙二醇干扰素α和利巴韦林治疗24～48周,针对丙肝病毒感染的新型特异性靶向抗病毒治疗正在临床研制中.

关键词：抗病毒治疗慢性丙型肝炎病毒感染丙肝病毒感染聚乙二醇干扰素α 诊断预防策略高危人群病毒清除疾病进展治疗方法

Computer Modelling of Compact 28/38 GHz Dual-Band Antenna for Millimeter-Wave 5G Applications

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Computer Modeling in Engineering & Sciences 2023年第12期137卷 2867-2879页

作者： Amit V.patel Arpan Desai Issa Elfergani Hiren Mewada Chemseddine Zebiri keyur Mahant Jonathan Rodriguez Raed Abd-Alhameed Department of Electronics and Communication Engineering Chandubhai S Patel Institute of Technology(CSPIT)Charotar University of Science and Technology(CHARUSAT)Changa388421India The Instituto de Telecomunica珲es Campus Universitário de SantiagoAveiro3810-193Portugal School of Engineering and Informatics University of BradfordBradfordBD71DPUK Electrical Engineering Department Prince Mohammad Bin Fahd UniversityAl Khobar31952Saudi Arabia Department of Electronics Laboratoire d’Electronique de Puissance et Commande Industrielle(LEPCI)University of Ferhat AbbasSétif19000Algeria

A four-element compact dual-band patch antenna having a common ground plane operating at 28/38 GHz is proposed formillimeter-wave communication systems in this ***-input-multiple-output(MIMO)antenna geometry consists of a slotted ellipse enclosed within a hollow circle which is orthogonally rotated with a connected partial ground at the *** overall size of the four elements MIMO antenna is 2.24λ×2.24λ(at 27.12GHz).The prototype of four-element MIMOresonator is designed and printed using Rogers RTDuroid 5880 withε_(r)=2.2 and loss tangent=0.0009 and having a thickness of 0.8 *** covers dual-band having a fractional bandwidth of 15.7%(27.12-31.34 GHz)and 4.2%(37.21-38.81 GHz)for millimeter-wave applications with a gain of more than 4 dBi at both *** proposed antenna analysis in terms ofMIMOdiversity parameters(Envelope Correlation Coefficient(ECC)and Diversity Gain(DG))is also carried *** experimental result in terms of reflection coefficient,radiation pattern,gain and MIMOdiversity parameter correlates very well with the simulated ones that show the potential of the proposed design for MIMO applications at millimeter-wave frequencies.

关键词： Connected ground diversity parameters dual-band antenna fifth generation(5G) mmwave multiple input multiple output(MIMO)

Trends in prevalence and mortality of gastroschisis and omphalocele in the United States from 2010 to 2018

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World Journal of Pediatrics 2022年第7期18卷 511-514页

作者： Parth Bhatt Frank Adusei Poku Jacob Umscheid Marian Ayensu Narendrasinh Parmar Rhythm Vasudeva keyur Donda Harshit Doshi Fredrick Dapaah-Siakwan United Hospital Center BridgeportWVUSA University of Central Oklahoma EdmondOKUSA University of Kansas School of Medicine WichitaKSUSA Woodhull Medical Center New YorkUSA Brookdale University Hospital and Medical Center BrooklynNYUSA University of South Florida TampaFLUSA Golisano Children's Hospital of Southwest Florida FortMyersFLUSA Neonatal Intensive Care Unit Valley Children's Hospital9300 Valley Children's PlaceSE 20MaderaCA 93636USA

Gastroschisis and omphalocele are the two most common congenital defects of the anterior abdominal *** etiologies remain unclear,but while gastroschisis is associ-ated with young maternal age,maternal smoking,maternal alcohol and illicit drug use during pregnancy omphalocele is frequently associated with genetic disorders and other congenital anomalies[1].The incidence of gastroschisis in the United States(US)has increased with an estimated inci-dence of 4.6 per 10,000 live births(LB)between 2010 and 2014[2].A recent study capturing information on 20%of infants admitted to neonatal intensive care unit(NICU)in the US showed a decrease in gastroschisis hospitalizations while that of omphalocele remained stable[3].However,it lacks generalizability[3].Our aim was to provide updated trends of gastroschisis and omphalocele hospitalizations in the US between 2010 and 2018 using population-based and nationally representative databases.

关键词： admitted halo maternal

Antioxidative,antimicrobial and anti-inflammatory activities and release of ultra-filtered antioxidative and antimicrobial peptides during fermentation of sheep milk:In-vitro,in-silico and molecular interaction studies

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Food Bioscience 2022年第3期47卷 583-594页

作者： Jodhani keyur Ashokbhai Bethsheba Basaiawmoit Sujit Das Amar Sakure Ruchika Maurya Mahendra Bishnoi Kanthi Kiran Kondepudi Srichandan Padhi Amit Kumar Rai Zhenbin Liu Subrota Hatia Deptt.of Dairy Microbiology Anand Agricultural UniversityAnand388110GujaratIndia Deptt.of Rural Development and Agricultural Production North-Eastern Hill UniversityTura CampusTura794002MeghalayaIndia Deptt.of Agriculture Biotechnology Anand Agricultural UniversityAnand388110GujaratIndia Regional Center for Biotechnology FaridabadHaryana121001India Healthy Gut Research Group Food & Nutritional Biotechnology DivisionNational Agri-Food Biotechnology InstituteKnowledge CitySector 81SAS NagarPunjab140306India Institute of Bioresources and Sustainable Development Regional CentreTadong737102SikkimIndia School of Food and Biological Engineering Shaanxi University of Science and Technology18Xi'an710021China

The antioxidative,antimicrobial,and anti-inflammatory properties of fermented sheep milk with *** (KGL4),as well as the generation of antioxidative and antimicrobial peptides,are evaluated in the *** and antibacterial activities in sheep milk fermented with KGL4 increased with incubation hours,along with varied antioxidative properties (ABTS assay:35.12%,hydroxyl free radical scavenging assay:29.12%,superoxide free radical scavenging activity:36.38%).Antimicrobial activity of fermented sheep milk against *** (19 mm),*** (15.67 mm),*** (14 mm),and *** (13 mm) was also ***,after 48 h,the KGL4 showed maximum proteolysis (10.40 mg/ml) at 2.5% rate of addition sheep *** antioxidative and antimicrobial activities of fermented sheep milk fractions (3 kDa and 10 kDa permeates and retentates) were also *** highest ABTS activity (26.90%) was found in 10 kDa permeate,whereas the 3 kDa retentate had higher hydroxyl free radical scavenging activity (69.20%) and 3 kDa permeate showed maximum superoxide free radical scavenging activity (32.85%).The 10 kDa retentate had shown maximum antimicrobial activity against *** (13.67 mm) and *** (17.00 mm).Proteins spotted on 2D gel electrophoresis of KGL4 were varied from 10 to 70 ***-LC/MS was used to identify 5 novel peptide sequences from 2-D gel *** in the BIOPEP database confirmed the antioxidative and antimicrobial effects of the novel fermented sheep milk *** sheep milk with KGL4 (SMKGL4) significantly reduced excessive TNF-α,IL-6,& IL-1β production in LPS-activated RAW 264.7 cells.

关键词： Fermentation Sheep milk Lactobacillus fermentum Antioxidative Antimicrobial Anti-inflammatory Peptides

Therapeutic potential of snake venom in cancer therapy:current perspectives

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Asian Pacific Journal of Tropical Biomedicine 2013年第2期3卷 156-162页

作者： Vivek Kumar Vyas keyur Brahmbhatt Hardik Bhatt Utsav Parmar Institute of Pharmacy Nirma UniversityAhmadabad 382481GujaratIndia Arihant School of Pharmacy and Bio Research Institute

Many active secretions produced by animals have been employed in the development of new drugs to treat diseases such as hypertension and *** venom toxins coutributed significantly to the treatment of many medical *** are many published studies describing and elucidating the anti-cancer potential of snake *** therapy is one of the main areas for the use of protein peptides and enzymes originating from animals of different *** of these proteins or peptides and enzymes from snake venom when isolated and evaluated may bind specifically to cancer cell membranes,affecting the migration and proliferation of these *** of substances found in the snake venom present a great potential as anti-tumor *** this review,we presented the main results of recent years of research involving the active compounds of snake venom that have anticancer activity.

关键词： Snake venom Cytotoxin Anticancer agents Apoptosis inducer

Scrupulous recognition of biologically important acids by fluorescent ＂turn off-on＂ mechanism of thaicalix reduced silver nanoparticles

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Chinese Chemical Letters 2017年第2期28卷 312-318页

作者： Savan M.Darjee keyur D.Bhatt Urvi S.Panchal Vinod K.Jain Department of Chemistry School of Sciences Gujarat University Ahmedabad 380009 India Department of Chemistry C.U. Shah University Wadhwan 363030 India

Water dispersible silver nanoparticles（AgNps） were prepared using thiacalix[4]arene tetrahydrazide（TCTH） as a reducing and stabilizing ***-AgNps were characterized by surface plasmon resonance（SPR）,transmission electron microscopy（TEM） and energy dispersive X-ray（EDX）.Relatively uniform 20 nm spherical particles of TCTH-AgNps were efficiently formed over a pH range of 5-9 and from 10-40 ℃.The interaction behavior of TCTH-AgNps with different amino acids was investigated using spectrophotometry and *** the amino acids tested,only tryptophan and histidine showed fluorescence quenching and fluorescence enhancement,*** linear detection range by Stern-Volmer plot was 5 nmol/L to 0.48 μmol/L for tryptophan and 4 nmol/L to 0.54 μmol/L for ***-AgNps were able to effectively reduce the levels of gram-positive bacteria,gram-negative bacteria,and *** properties argue for the potential use of TCTH-AgNps as detectors of histidine and tryptophan and as antibiotics.

关键词： Silver nanoparticles Fluorescent sensor Colorimetric sensor Thiacalixarene