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Ischemic preconditioning enhances hepatocyte proliferation in the early phase after ischemia under hemi-hepatectomy in rats

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hepatobiliary & pancreatic Diseases International 2012年第5期11卷 521-526页

作者： Li-Ming Jin, Shui-Fang Jin, Yuan-Xing Liu, Lin Zhou, Hai-Yang Xie, Sheng Yan, Xiao Xu , Shu-Sen Zheng Department of General surgery and Intensive Care Unit, first affiliated hospital, zhejiang Chinese Medical university, hangzhou 310006, china key laboratory of combined multi-organ transplantation, ministry of public health, key laboratory of organ transplantation, zhejiang province, and division of hepatobiliary and pancreatic surgery, first affiliated hospital, zhejiang university school of medicine, hangzhou 310003, china [a] Department of General surgery First Affiliated Hospital Zhejiang Chinese Medical University Hangzhou 310006 Hangzhou China [b] Intensive Care Unit First Affiliated Hospital Zhejiang Chinese Medical University Hangzhou 310006 Hangzhou China [c] key laboratory of combined multi-organ transplantation Ministry of Public Health Key Laboratory of Organ Transplantation Zhejiang Province and Division of Hepatobiliary and Pancreatic Surgery First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 Hangzhou China

BACKGROUND: Ischemia/reperfusion (I/R) injury is an important barrier to liver surgery and transplantation because it impairs remnant liver/reduced-size-graft regeneration. Ischemic preconditioning (IPC), as an effective measure to overcome I/R injury, has been shown to enhance the regenerative capacity of hepatocytes. However, investigations have always focused on regeneration in the late phase after reperfusion. This study aimed to investigate whether IPC enhances hepatocyte proliferation in the early phase after reperfusion and possible underlying mechanisms. METHODS: A total of 90 rats were divided into three groups: hemi-hepatectomy alone (PHx group), 60 minutes of ischemia plus hemi-hepatectomy (I/R group), and a cycle of 10 minutes of alternating I/R prior to 60 minutes of ischemia plus hemi-hepatectomy (IPC group). Each group was divided into five subgroups sacrificed after 0.5, 2, 6, 12 or 24 hours (n=6/ subgroup). Subsequently, serum concentrations of alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) were measured; caspase-3 and proliferating cell nuclear antigen (PCNA) proteins were also determined by Western blotting. Furthermore, PCNA was detected by immunohistochemistry to identify the expression ***: Serum ALT and AST levels after 2-24 hours of reperfusion in the PHx and IPC groups were remarkably decreased compared to the I/R group, and the serum TNF-α was relatively lower. A significant increase of serum IL-6 levels was found in the PHx and IPC groups compared with the I/R group at each time point. Furthermore, PCNA expression was remarkably increased in the IPC group after 6-12 hours of reperfusion, and in the earlier 0.5 and 6 hours time points after reperfusion have shown the massive PCNA-positive hepatocytes. At the same time, the expression of liver p-JNK was higher in the IPC group in the early phase after reperfusion than that of the I/R group and its expre

关键词： reperfusion injury ischemic preconditioning hepatocytes proliferation hemi-hepatectomy

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Expression of HLA-G in patients with hepatocellular carcinoma

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hepatobiliary & pancreatic Diseases International 2011年第2期10卷 158-163页

作者： Yan Wang,Zhou Ye,Xue-Qin Meng and Shu-Sen Zhengdivision of hepatobiliary and pancreatic surgery key laboratory of combined multi-organ transplantation,ministry of public health and key laboratory of organ transplantation zhejiang province, first affiliated hospital,zhejiang university school of medicine,hangzhou 310003,china division of hepatobiliary and pancreatic surgery key laboratory of combined multi-organ transplantation Ministry of Public Health and key laboratory of organ transplantation zhejiang province First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China

BACKGROUND:Human leukocyte antigen G(HLA-G)is a non-classical major histocompatibility complex class I molecule that has multiple immune regulatory functions including the induction of immune *** detection of HLA-G expression might serve as a clinical marker in the prediction of clinical outcomes for certain types of ***, we investigated whether or not HLA-G is also expressed in patients with hepatocellular carcinoma(HCC),and whether the expression has clinical value. METHODS:Serum levels of secreted HLA-G(sHLA-G)were measured by ELISA in 36 patients with HCC,25 patients with liver cirrhosis(LC)and 25 healthy *** expression of HLA-G in liver tissue was further studied using Western blotting in 36 patients with HCC and 25 with *** correlations between HLA-G status and various clinicopathological parameters including survival were analyzed. RESULTS:The ELISA assay showed that the serum levels of sHLA-G in the HCC,LC and healthy groups were 132.6± 31.4,63.5±22.1,and 47.0±15.5 U/ml,*** of variance was used for inter-group comparison and differences were found between the HCC group and the other two groups (both Phealthy group(P=0.112).HLA-G protein expression in liver tissue was found in 66.7%(24/36)of the primary sites of HCC,but not in benign lesions(LC). Further,the HLA-G expression in tumors had no significant correlation with the parameters of age,gender,histological grade and alpha-fetoprotein ***,patients with HLA-G-positive tumors had a shorter postoperative survival time than those with HLA-G-negative tumors(P=0.014).Also, univariate analysis showed that HLA-G was an independent prognostic ***:Our results indicated that the expression of HLA-G was a characteristic feature of HCC and patients with positive expression of HLA-G in malignant liver tissue had a poor prognosis.

关键词： human leukocyte antigen G hepatocellular carcinoma prognosis

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Risk factors and incidence of acute pyogenic cholangitis

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hepatobiliary & pancreatic Diseases International 2012年第6期11卷 650-654页

作者： Yun-Sheng Qin, Qi-Yong Li, Fu-Chun Yang , Shu-Sen Zheng division of hepatobiliary and pancreatic surgery key laboratory of combined multi-organ transplantation, ministry of public health key laboratory of organ transplantation zhejiang province, first affiliated hospital, zhejiang university school of medicine, hangzhou 310003, china division of hepatobiliary and pancreatic surgery key laboratory of combined multi-organ transplantation Ministry of Public Health and key laboratory of organ transplantation zhejiang province First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China

BACKGROUND: Acute cholangitis varies from mild to severe form. Acute suppurative cholangitis (ASC), the severe form of acute cholangitis, is a fatal disease and requires urgent biliary decompression. Which patients are at a high risk of ASC and need emergency drainage is still unclear. The present study aimed to identify the factors for determining early-stage ASC and distinguishing ASC from acute cholangitis. METHODS: We analyzed 359 consecutive patients with acute cholangitis who had been admitted to the first affiliated hospital, zhejiang university school of medicine from January 2004 to May 2011. Emergency endoscopic retrograde cholangiopancreatography (ERCP) was carried out in all patients to decompress or clear the stones by experienced endoscopists Clinical and therapeutic data were collected, and univariate and multivariate analyses were performed to identify the potential risk factors of ASC. RESULTS: Of the 359 patients, 1 was excluded because of failure of ERCP drainage. Of the remaining 358 patients with an average age of 62.7 years (range 17-90), 162 were diagnosed with ASC, and 196 with non-ASC. ENBD catheters were placed in 343 patients (95.8%), of whom 182 patients had stones removed at the same time, and plastic stent was placed in 25 patients (7.0%) Clinical conditions were improved quickly after emergency biliary drainage in all patients. Complications were identified in 11 patients (3.1%): mild pancreatitis occurred in 8 patients and hemorrhage in 3 patients. There was no mortality. Univariate analysis showed that several variables were associated with ASC: age, fever, decreased urine output, hypotension, tachycardia abnormal white blood cell count (WBC), low platelet, high C reactive protein (CRP), and duration of the disease. multivariateanalysis revealed that advanced age, hypotension, abnormal WBC, high CRP, and duration of the disease were independent risk factors for ASC. CONCLUSIONS: This study demonstrates that advanced age, hypotension,

关键词： acute cholangitis suppurative endoscopic retrograde cholangiopancreatography

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Evaluation of hepatitis B viral replication and proteomic analysis of HepG2.2.15 cell line after knockdown of HBx

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hepatobiliary & pancreatic Diseases International 2011年第3期10卷 295-302页

作者： Hai-Yang Xie,Jun Cheng,Chun-Yang Xing,Jin-Jin Wang,Rong Su,Xu-Yong Wei,Lin Zhou and Shu-Sen Zheng Author Affiliations:key laboratory of combined multi-organ transplantation,ministry of public health key laboratory of organ transplantation zhejiang province and division of hepatobiliary and pancreatic surgery,Department of surgery,first affiliated hospital,zhejiang university school of medicine,hangzhou 310003,china key laboratory of combined multi-organ transplantation Ministry of Public Health key laboratory of organ transplantation zhejiang province and division of hepatobiliary and pancreatic surgery Department of Surgery First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China

BACKGROUND:Hepatitis B virus (HBV) is one of the major pathogens of human liver *** have shown that HBV X protein (HBx) plays an important role in promoting viral gene expression and *** this study we performed a global proteomic profiling to identify the downstream functional proteins of HBx,thereby detecting the mechanisms of action of HBx on virion ***:HBx in the HepG2.2.15 cell line was knocked down by the transfection of small interfering RNA (siRNA).The replication level of HBV was evaluated by microparticle enzyme immunoassay analysis of HBsAg and HBeAg in the culture supernatant,and real-time quantitative PCR analysis of HBV ***-dimensional electrophoresis combined with MALDI-TOF/TOF was performed to analyze the changes in protein expression profile after treatment with HBx ***:Knockdown of HBx disturbed HBV replication in *** target siRNA significantly inhibited the expression of HBsAg,HBeAg and the replication of HBV *** significantly changed proteins (7 upregulated and 5 downregulated) were successfully identified by MALDI-TOF/TOF using proteomics differential expression analysis after the knockdown of *** these identified proteins,HSP70 was validated by Western ***:The results of the study indicated the positive effect of HBx on HBV replication,and a group of downstream target proteins of HBx may be responsible for this effect.

关键词： hepatitis B virus HBx replication proteomics

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Liver transplantation in Crigler-Najjar syndrome type I disease

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hepatobiliary & pancreatic Diseases International 2012年第5期11卷 545-548页

作者： Zhen-Hua Tu, De-Sheng Shang, Jin-Cai Jiang, Wu Zhang, Min Zhang, Wei-Lin Wang, Hai-Yan Lou,Shu-Sen Zheng division of hepatobiliary and pancreatic surgery, Department of surgery, first affiliated hospital, zhejiang university school of medicine, key laboratory of combined multi-organ transplantation, ministry of public health & key laboratory of organ transplantation of zhejiang province Department of Radiology,first affiliated hospital,zhejiang university school of medicine, hangzhou 310003, china [a] division of hepatobiliary and pancreatic surgery Department of Surgery First Affiliated Hospital Zhejiang University School of Medicine Key Laboratory of Combined Multi-Organ Transplantation Ministry of Public Health & Key Laboratory of Organ Transplantation of Zhejiang Province Hangzhou 310003 Hangzhou China [b] Department of Radiology First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 Hangzhou China

BACKGROUND: Crigler-Najjar syndrome type I (CNS I) is a very rare autosomal recessive inherited disease that liver transplantation can properly deal with. METHODS: We present one case of an 18-month-old child with CNS I diagnosed by clinical findings and genetic detecting LTx was performed 5 days after kernicterus broke out and neurological symptoms were successfully reversed. RESULT: Magnetic resonance imaging and magnetic resonance spectroscopy showed encouraging results that brain pathology had a trend to return to normal in 1-year follow-up, combined with electroencephalogram and motor development estimate studies. CONCLUSIONS: Liver transplantation can cure CNS I with reversible neurological symptoms to some extent in time Magnetic resonance spectroscopy may be a future option of predicting brain conditions and selecting suitable patients with CNS I for transplantation.

关键词： Crigler-Najjar liver transplantation inborn error of metabolism magnetic resonance imaging hyperbilirubinemia

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Effects of suppressing glucose transporter-1 by an antisense oligodeoxynucleotide on the growth of human hepatocellular carcinoma cells

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hepatobiliary & pancreatic Diseases International 2011年第1期10卷 72-77页

作者： Tian-Qi Liu,Jun Fan,Lin Zhou and Shu-Sen Zheng key laboratory of combined multi-organ Trans-plantation,ministry of public health key laboratory of organ Trans-plantation,zhejiang province and division of hepatobiliary and pancreatic surgery,Department of surgery State key laboratory for Diagnosis and Treatment of Infectious Disease,first affiliated hospital,zhejiang university school of medicine,hangzhou 310003,china Department of hepatobiliary surgery,the People’s hospital of Guangxi Zhuang Autonomous Region, Nanning 530021,china [a] key laboratory of combined multi-organ transplantation Ministry of Public Health key laboratory of organ transplantation Zhejiang Province and division of hepatobiliary and pancreatic surgery Department of Surgery Zhejiang University School of Medicine Hangzhou 310003 China [b] State key laboratory for Diagnosis and Treatment of Infectious Disease First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China [c] Department of hepatobiliary surgery the People's Hospital of Guangxi Zhuang Autonomous Region Nanning 530021 China

BACKGROUND:The glucose transporter-1(Glut-1),a key ratelimiting factor in the transport and metabolism of glucose in cancer cells,is over-expressed in many human cancer cells and this overexpression is correlated with poor biological behavior. The increased levels of Glut-1 expression in hepatocellular carcinoma(HCC)cells functionally affect *** study was undertaken to investigate effects of suppressing Glut-1 by an antisense oligodeoxynucleotide(AS-ODN)on the growth of human hepatocellular carcinoma(HepG-2)cells. METHODS:We used AS-ODN targeting against the Glut-1 gene in a HepG-2 cell *** were four experimental groups: empty pcDNA3.1 vector(mock transfection),pcDNA3.1-anti-Glut(+),pcDNA3.1-Glut(+),and non-transfected HepG-2 cells. The Glut-1 mRNA expression was detected by RT-PCR and the Glut-1 protein expression by Western blotting after cell culture, and the glucose uptake was detected after glucose stimulation in each group. RESULTS:Compared with non-transfected HepG-2 or Glut-1 pcDNA3.1,a down-regulation of Glut-1 mRNA in HepG-2 cells transfected with anti-Glut-1 pcDNA3.1 was noted(P<0.05).Glut-1 protein in HepG-2 cells transfected with Glut-1 AS-ODN was decreased compared with non-transfected HepG-2,Glut-1 pcDNA3.1,or empty vectors. Glucose uptake by the HepG-2 cells transfected with AS-ODN was decreased at 1 hour after glucose ***:The application of Glut-1 AS-ODN can down-regulate the expression of Glut-1 at mRNA and protein,and inhibit glucose uptake partially in HepG-2 *** Glut-1 gene maybe a potential therapeutic target for HCC.

关键词： hepatocellular carcinoma HepG-2 cell glucose transporter-1 therapeutic target

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Enteral supplementation with glycyl-glutamine improves intestinal barrier function after liver transplantation in rats

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hepatobiliary & pancreatic Diseases International 2011年第4期10卷 380-385页

作者： Jian-Wen Jiang,Zhi-Gang Ren,Lu-Yan Chen,Li Jiang,Hai-Yang Xie,Lin Zhou and Shu-Sen Zheng key laboratory of combined multi-organ transplantation,ministry of public health Department of hepatobiliary and pancreatic surgery,first affiliated hospital,zhejiang university school of medicine,hangzhou 310003,china key laboratory of combined multi-organ transplantation Ministry of Public Health Department of hepatobiliary and pancreatic surgery First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China

BACKGROUND:Most patients after liver transplantation (LT) suffer from intestinal barrier ***-glutamine (Gly-Gln) by parenteral supplementation is hydrolyzed to release glutamine,which improves intestinal barrier function in intestinal *** study aimed to investigate the effect of GlyGln by enteral supplementation on intestinal barrier function in rats after allogenetic LT under immunosuppressive ***:Twelve inbred Lewis rats were selected randomly as donors,and 24 inbred Brown Norway (BN) rats as recipients of allogenetic *** recipients were divided into a control group (Ala,n=12) and an experimental group (Gly-Gln,n=12).In each group,6 normal BN rats were sampled for normal parameters on preoperative day *** 6 recipients in the control group received alanine (Ala) daily by gastric perfusion for 3 preoperative days and 7 postoperative days,and the 6 recipients in the experimental group were given Gly-Gln in the same *** 12 BN recipients underwent orthotopic LT under sterile conditions after a 3-day fast and were given immunosuppressive therapy for 7 *** were harvested for sampling on postoperative day *** following parameters were assessed:intestinal mucosal protein content,mucosal ultrastructure,ileocecal sIgA content,portal plasma levels of endotoxin and TNF-α,and bacterial ***:All recipients were alive after *** preoperative day 3,all parameters were similar in the two *** postoperative day 8,all parameters in the two groups were remarkably changed from those on preoperative day ***,compared to the Ala group,supplementation withGly-Gln increased the levels of intestinal mucosal protein and ileocecal sIgA,improved mucosal microvilli,and decreased portal plasma levels of endotoxin and TNF-α as well as bacterial ***:Enteral supplementation with Gly-Gln improved intestinal barrier function after allogenetic LT in rats.

关键词： liver transplantation glycyl-glutamine bacterial translocation intestinal barrier function

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Polymorphisms of CCL3L1/CCR5 genes and recurrence of hepatitis B in liver transplant recipients

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hepatobiliary & pancreatic Diseases International 2011年第6期10卷 593-598页

作者： Hong Li,Hai-Yang Xie,Lin Zhou,Wei-Lin Wang,Ting-Bo Liang,Min Zhang and Shu-Sen Zheng key laboratory of combined multi-organ Transplan-tation,ministry of public health,and division of hepatobiliary pancreatic surgery,first affiliated hospital,zhejiang university school of medicine,hangzhou 310003,china key laboratory of combined multi-organ transplantation Ministry of Public Health Zhejiang University School of Medicine Hangzhou 310003 China division of hepatobiliary pancreatic surgery First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China

BACKGROUND:The genetic diversity of chemokines and chemokine receptors has been associated with the outcome of hepatitis B virus *** aim of this study was to evaluate whether the copy number variation in the CCL3L1 gene and the polymorphisms of CCR5Δ32 and CCR5-2459A→G (rs1799987) are associated with recurrent hepatitis B in liver transplantation for hepatitis B virus infection-related end stage liver ***:A total of 185 transplant recipients were enrolled in this *** genomic DNA was extracted from whole blood,the copy number of the CCL3L1 gene was determined by a quantitative real-time PCR based assay,CCR5Δ32 was detected by a sizing PCR method,and a single-nucleotide polymorphism in CCR5-2459 was detected by restriction fragment length polymorphism ***:No CCR5Δ32 mutation was detected in any of the individuals from *** copy number variation nor polymorphism in CCR5-2459 was associated with post-transplant reinfection with hepatitis B ***,patients with fewer copies (transplantation.

关键词： CCL3L1 chemokine receptor 5 copy number variation hepatitis B liver transplantation

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Protective effect of probiotics on intestinal barrier function in malnourished rats after liver transplantation

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hepatobiliary & pancreatic Diseases International 2011年第5期10卷 489-496页

作者： Zhi-Gang Ren, Hui Liu, Jian-Wen Jiang, Li Jiang, Hui Chen, Hai-Yang Xie, Lin Zhou and Shu-Sen Zheng key laboratory of combined multi-organ Transplan- tation, ministry of public health, and Department of hepatobiliary and pancreatic surgery, first affiliated hospital, zhejiang university school of medicine, hangzhou 310003, china and Institute of Immunology, zhejiang university school of medicine, hangzhou 310058, china [a] key laboratory of combined multi-organ transplantation Ministry of Public Health and Department of Hepatobiliary and Pancreatic Surgery First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China [b] Institute of Immunology Zhejiang University School of Medicine Hangzhou 310058 China

BACKGROUND: Most patients waiting for liver transplantation have end-stage liver diseases with malnutrition, which is prone to induce intestinal barrier dysfunction after liver transplantation. We aimed to study the effect of probiotics on intestinal barrier function in malnourished rats following liver transplantation with long-term antibiotics. METHODS: Twelve Lewis rats were selected as donors. Twelve BN rats, which served as recipients, were subjected to malnutrition by semi-starvation for 4-5 weeks. They were randomly divided into two groups: a control group which received phosphate-buffered saline and a probiotics group which received Bifidobacterium and Lactobacillus. All recipients were injected with intramuscular imipenem and subcutaneous cyclosporine A. Furthermore, six normal BN rats without any drugs or operations served as a normal group. Eight days after operation, all rats were sacrificed for examination of the following parameters: serum levels of endotoxin and TNF-α, bacterial translocation, intestinal microflora, ileocecal sIgA, lymphocyte numbers, and phenotypes (CD4, CD8, αβTCR, γδTCR)ofPeyer’spatches. RESULTS: In recipients subjected to malnutrition, weight decreased by 20% and they survived until 8 days after operation. Compared with the normal group, all recipients on postoperative day 8 showed increased levels of serum endotoxin and TNF-α as well as increased counts oftranslocated bacteria. Meanwhile, there were decreases in counts of Bifidobacterium and Lactobacillus in the ileocecum, sIgA concentration, and lymphocytes of Peyer’s patches. Moreover, partial alteration in lymphocyte phenotypes was evidenced by elevated ratios of CD8 + and γδTCR + lymphocytes. In contrast, compared to the control group, supplementation with probiotics reduced the levels of serum endotoxin, TNF-α and bacterial translocation, increased the counts of Bifidobacterium and Lactobacillus, the concentration of sIgA and lymphocytes of Peyer’s patches, and also slightly

关键词： liver transplantation probiotics intestinal microflora intestinal barrier function

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Effect of 1,25-dihydroxyvitamin D3 on preventing allograft from acute rejection following rat orthotoic liver transplantation

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World Journal of Gastroenterology 2003年第5期9卷 1067-1071页

作者： Ai-Bin Zhang Shu-Sen Zheng Chang-Ku Jia Yan Wang Department of hepatobiliary pancreatic surgery,The first affiliated hospital of medicine school,zhejiang university,key Lab of combined multi-organ transplantation,ministry of public health,hangzhou 310003,zhejiang province,china Ai-Bin Zhang Shu-Sen Zheng Chang-Ku Jia Yan Wang Department of Hepatobiliary Pancreatic Surgery The First Affiliated Hospital of Medicine School Zhejiang University Key Lab of Combined Multi-Organ Transplantation Ministry of Public Health Hangzhou 310003 Zhejiang Province China

AIM: To study the mechanism and the preventive role of 1,25-dihydroxyvitamin D3 in acute rejection following orthotopic liver ***: Rats were randomly divided as donors or recipients for orthotopic liver allotransplantation model. Four groups were designed in the study, Group Ⅰ: syngenic control (Wistar to Wistar); Group Ⅱ: acute rejection (SD to Wistar);Group Ⅲ: acute rejection treated with cyclosporine A, and Group Ⅳ: acute rejection treated with 1,25-(OH)2D3. Liver function, rejection activity index and mRNA of IFN-γ, IL-10intragraft in recipients were measured on day t, 5, 7, 15,30 posttransplant for assessing graft function, severity of acute rejection and immune state of ***: Survival time of recipients in Group Ⅳ was significantly prolonged (4/6 recipients survived for over 100days. vs Group Ⅱ, P＜0.001; vs Group Ⅲ, P＞0.05). After treatment with 1,25-(OH)2 D3, mean value of all the assay tested on each experimental time was compared, liver function in group Ⅳ was significantly improved (AST 127±41U/L-360±104 U/L, BIL 13±5 mmol/1-38±11 mmol/l; vs Group Ⅱ, P＜0.05; vs Group Ⅲ, P＞0.05. Rejection activity index was significantly decreased (0-3.3±1.6; vsGroup Ⅱ, P＜0.05;vsGroup Ⅲ, P＞0.05). Level of hepatic IFN-γ, mRNA in group Ⅳ was decreased, while level of hepatic IL-10 mRNA was increased (vs Group Ⅱ, P＜0.05; vs Group Ⅲ, P＞0.05).CONCLUSION: Our results indicated that 1,25-(OH)2D3induced the secretion of cytokine toward to Th2 type, which would alleviate acute rejection, protect liver function and prolong survival of recipient after orthotopic liver transplantation.

关键词： 1,25-二羟基维生素D3 同种异体移植急性排斥反应矫形肝移植术动物实验作用机制

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