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从植物科学到农业发展——2012全国植物生物学大会

作者： Jiangbo Fan,Wei Liu,Qun Li,Yu’e Zhang,Yongbiao Xue~* State key laboratory of molecular Developmental Biology,{3. of Genetics and Developmental Biology,chinese academy of sciences and National Center for Plant Gene Research,Beijing 100101.

Self-incompatibility(SI) is an intra-specific reproductive barrier to prevent self-fertilization and to promote cross-fertilization found in flowering *** the SI response,self or genetically related pollen is rejected by the pistil and non-self(cross) pollen accepted for *** outcomes are referred to as self-pollen incompatibility(SPI) and cross-pollen compatibility(CPC),*** studies show that a single polymorphic S-locus,encoding at least two components from both the pollen and pistil sides,controls the discrimination of self and cross *** studies have revealed that plants use several distinct molecular mechanisms to accomplish the SI *** them,the Solanaceae,Plantaginaceae and Rosaceae appear to share a similar molecular *** these families,the SI specificity is determined by a ribonuclease protein (S-RNase) expressed in the pistil and a cluster of S-Locus F-box protein(SLF/SFB) in *** is widely accepted that S-RNases are secreted by the pistil and taken up by growing pollen tubes and function as cytotoxins to arrest self-pollen tube ***, the biochemical and cellular mechanisms remain largely *** this talk,I will present our recent data showing where and how S-RNase and SLF interact with each other to control CPC and SPI responses in Petunia hybrid,a solanaceous species.

关键词： self-incompatibility S-RNase SLF Petunia hybrida

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Enzymatic Characterization of a Monomeric Isocitrate Dehydrogenase from Streptomyces lividans TK54

Enzymatic Characterization of a Monomeric Isocitrate Dehydro...

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华东六省一市生物化学与分子生物学会2009年学术交流会

作者： Beibei Zhang Baojuan Wang Peng Wang Zhengyu Cao Guoping Zhu* (key laboratory of molecular {3. and Biodiversity and institute of molecular Biology and Biotechnology,Anhui Normal University,Wuhu 241000,China)

＜正＞Isocitrate dehydrogenase（IDH） is one of the key enzymes in the citric acid cycle,which involves in providing energy and biosynthetic precursors for ***,we report for the first time the enzymatic characterizati...

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Snapping magnetosome chains by asymmetric cell division in magnetotactic bacteria

Snapping magnetosome chains by asymmetric cell division in m...

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中国科学院地质与地球物理研究所第11届（2011年度）学术年会

作者： Wei Lin~(1,2) and Yongxin Pan~(1,2*) 1 Biogeomagnetism Group,Paleomagnetism and Geochronology laboratory,key laboratory of the Earth’s Deep Interior,{3. of Geology and Geophysics,chinese academy of sciences,Beijing 100029,China. 2 France-China Bio-Mineralization and Nano-Structures laboratory,chinese academy of sciences,Beijing 100029,China.

Summary The mechanism by which prokaryotic cells organize and segregate their intracellular organelles during cell division has recently been the subject of substantial *** other microorganisms,magnetotactic bacteria(MTB) form internal magnets(known as mag-netosome chain) for magnetic orientation,and thus face an additional challenge of dividing and equiparti-tioning this magnetic receptor to their daughter cells. Although MTB have been investigated more than four decades,it is only recently that the basic mechanism of how MTB divide and segregate their magnetic organelles has been *** this issue of molecular Microbiology,the cell cycle of the model magnetotactic bacterium,Magnetospirillum gryph-iswaldense is characterized by Katzmann and *** authors have found that ***-iswaldense undergoes an asymmetric cell division along two planes.A novel wedge-like type of cellular constriction is observed before separation of daughter cells and magnetosome chains,which is assumed to help cell cope with the magnetic force within the mag-netosome *** data shows that the magneto- some chain becomes actively recruited to the cellular division site,in agreement with the previous suggestions described by Staniland et al.(2010),and the actin-like protein MamK is likely involved in this fast polar-to-midcell *** the use of cryo-electron tomography,an arc-shaped Z ring is observed near the division site,which is assumed to trigger the asymmetric septation of cell and magneto-some chain.

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Are nuclear loci ideal for barcoding plants? A case study of genetic delimitation of two sister species using multiple loci and multiple intraspecific individuals

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Journal of Systematics and evolution 2011年第3期49卷 182-188页

作者： 1Qian WANG 1,2Qiu-Shi YU 1Jian-Quan LIU* 1(molecular Ecology Group,key laboratory of Arid and Grassland Ecology,College of Life {3.,Lanzhou University,Lanzhou 73.000,China) 2(State key laboratory Breeding Base of Desertification and Aeolian Sand Disaster Combating,Gansu Desert Control Research institute,Lanzhou 73.070,China) (molecular Ecology Group Key Laboratory of Arid and Grassland Ecology College of Life Sciences Lanzhou University Lanzhou 730000 China) (State key laboratory Breeding Base of Desertification and Aeolian Sand Disaster Combating Gansu Desert Control Research Institute Lanzhou 730070 China) *Correspondence: Jian-Quan LIU *Correspondence: ?Author for correspondence. E-mail: liujq@*** Tel.: 86-93.1891-43.5 Fax: 86-97-1614-3.82.

Considerable debate remains as to which DNA region should be used to barcode *** different chloroplast(cp) DNA regions(rbcL,matK,and trnH-psbA) and nuclear ribosomal internal transcribed regions(ITS) have been suggested as suitable barcodes in ***,low-copy nuclear loci were also suggested to be potentially ideal barcode *** aim of the present study was to test the effectiveness of these proposed DNA fragments and five additional low-copy loci(CHS,DET1,COP1,PGIC1,and RPS2;comprising both coding and non-coding regions) in barcoding closely related *** examined the divergences within and between two species of Pugionium(Brassicaceae).We failed to find any interspecific variation from three cpDNA fragments with which to discriminate the two ***,a single base mutation in the internal transcribed spacer(ITS) could discriminate between the two species *** found more variations among all individuals of the two species using each of the other five low-copy nuclear ***,only alleles from one locus(DET1) of the five low-copy loci related to ?owering regulations was able to distinguish the sampled individuals into two *** failed to amplify the corresponding fragments out of Brassicaceae using the designed DET1 *** further discussed the discrimination power of different loci due to incomplete lineage sorting,gene ?ow,and species-specific *** results highlight the possibility of using the nuclear ITS as a core or complementary fragment to barcode recent diverged species.

关键词： chloroplast DNA DNA barcoding internal transcribed spacer(ITS) low-copy nuclear loci Pugionium.

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Efficient indica and japonica rice identification based on the InDel molecular method:Its implication in rice breeding and evolutionary research

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Progress in Natural Science:Materials International 2009年第10期19卷 1241-1252页

作者： Bao-Rong Lu,Xingxing Cai,Xin Jin Ministry of Education key laboratory for Biodiversity Science and Ecological Engineering,institute of Biodiversity Science,Fudan University,Shanghai 20043.,China [a]Ministry of Education key laboratory for Biodiversity Science and Ecological Engineering Institute of Biodiversity Science Fudan University Shanghai 200433 China

An efficient molecular method for the accurate and efficient identification of indica and japonica rice was created based on the poly-morphisms of insertion/deletion(InDel) DNA fragments obtained from the basic local alignment search tool(BLAST) to the entire genomic sequences of indica(93.11) and japonica rice(Nipponbare).The 45 InDel loci were validated experimentally by the polymerase chain reaction(PCR) and polyacrylamide gel electrophoresis(PAGE) in 44 typical indica and japonica rice varieties,including 93.11 and Nipponbare.A neutrality test of the data matrix generated from electrophoretic banding patterns of various InDel loci indicated that 3. InDel loci were strongly associated with the differentiation of indica and japonica *** extensive analyses involving cultivated rice varieties from 11 Asian countries,and 12 wild Oryza species with various origins confirmed that indica and japonica characteristics could accurately be determined via calculating the average frequency of indica-or japonica-specific alleles on different InDel loci across the rice *** method was named as the ’’InDel molecular index" that combines molecular and statistical methods in determining the indica and japonica characteristics of rice *** with the traditional methods based essentially on morphology,the InDel molecular index provides a very accurate,rapid,simple,and efficient method for identifying indica and japonica *** addition,the InDel index can be used to determine indica or japonica characteristics of wild Oryza species,which largely extends the utility of this *** InDel molecular index provides a new tool for the effective selection of appropriate indica or japonica rice germplasm in rice *** also offers a novel model for the study of the origin,evolution,and genetic differentiation of indica and japonica rice adapted to various environmental changes.

关键词： Cultivated rice Wild Oryza InDel molecular marker indica-japonica identification Neutrality test

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Proteomics of TGF-β1 induced concomitant apoptosis and EMT in AML12 cells

Proteomics of TGF-β1 induced concomitant apoptosis and EMT ...

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华东六省一市生物化学与分子生物学学会2006年学术交流会

作者： Hong-Yu Tian,Ke-Hua Zhang,Xia Gao,Jian-Feng lin, Gen-Jun Xu,Jian-Guo Song, Fu-Kun Zhao (key laboratory of Proteomics, institute of Biochemistry and Cell Biology, Shanghai institutes for Biological sciences, chinese academy of sciences, Shanghai, China laboratory of molecular Cell Biology, institute of Biochemistry and Cell Biology, Shanghai institutes for Biological sciences, chinese academy of sciences. 3.0 Yue-Yang Road, Shanghai 20003.,China College of Life Science,Zhejiang Sci-Tech University,Hangzhou 3.0018, China)

Introduction Transforming growth factor-beta (TGF-β1) conveys regulatory signals through multiple intracellular pathways, subsequently affecting various cellular functions. Apoptosis and epithelial-to-mesenchymal transition (EMT) have been implicated in a variety of biological processes, such as embryonic development,fibrosis and tumor progression. TGF-β1induces concomitant apoptosis and EMT in some epithelial cells, for example AML12 mouse epithelial cell. However, the mechanism that underlie TGF-β1induced the two different processes remain unclear. Here, we studied the changes in the proteome of AML12 cells in response to TGF-β1 treatment. 40 protein spots, affected by TGF-β1, were selected and identified by MOLDI-TOF/TOF. In these spots, 22 of them were upregulated and 18 of them were downregulated. Among them, there are proteins regulating cytoskeleton organization, energy metabolism, and signal transduction. These results provide a novel perspective for the mechanism of TGF-β signaling pathway in apoptosis and EMT.

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Echinacoside attenuates 6-hydroxydopamine-induced neurotoxicity in PC12 cells

Echinacoside attenuates 6-hydroxydopamine-induced neurotoxic...

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第二届世界天然药物和传统药物药理学学术会议

作者： Yue-Hua WANG~(1,2),Shuo TIAN~2,Guo-Rong HE~2,Lian-Hua FANG~2,Xiao-Ping PU~(1*),Guan-Hua DU~(2*) (1.Department of molecular and cellular Pharmacology,School of Pharmaceutical sciences,Peking University, Beijing 100191,China 2.Beijing {3. laboratory of Drug Target Identification and Drug Screening, institute of Materia Medica,chinese academy of Medical sciences & Peking Union Medical College, Beijing 100050,China)

＜正＞AIM To investigate the effects and the underlying mechanism of Echinacoside（ECH） against 6-OHDA-induced injury in *** 6-OHDA was added to cultured PC12 cells after pretreatment for 1 h with different concentration of ECH or saline and co-incubated for another 24 *** evaluate the protective effects of ECH,the morphological change was observed by microscope,the viability of PC12 cells was assayed by MTT *** explore the mechanism of ECH against 6-OHDA neurotoxicity, the mitochondrial oxidation-reduction activity（REDOX）,mitochondrial membrane potential（△ψm）,reactive oxygen species（ROS） production,ATP level,cytochrome c release,the activity of caspase 3.and apoptosis in PC12 cells were *** 6-OHDA induced the decrease of cell viability,ROS generation increase,ATP content decrease,mitochondrial dysfunction,and mitochondria -mediated apoptosis in PC12 *** could attenuate 6-OHDA-induced neurotoxicity,inhibit the collapse of△ψm,reduce the mitochondrial *** also inhibited cyt c release and caspase 3.*** Our results indicate that ECH attenuates 6-OHDA-induced neurotoxicity through anti-oxidative and anti-apoptosis mediated by mitochondria.

关键词： echinacoside Parkinson’s disease 6-hydroxydopamine

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MiR-124 is required for proper cell proliferation in early development of Xenopus eye and brain

MiR-124 is required for proper cell proliferation in early d...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Kai-Li LIU,Wei-Chuan MO,Ying LIU,Xiu-Mei WANG,Rong QIU,Rong-Qiao HE 1 The State key laboratory of Brain and Cognitive Science,institute of Biophysics,{3. academy of sciences, Beijing 100101,China 2 Graduate School of the chinese academy of sciences,Beijing 10003.,China

＜正＞Objective MiR-124 is one of the most abundant miRNAs enriched in developing and adult brain and eye.A recent report showed that miR-124 regulated adult neurogenesis in mammalian brain;however,its function in neural development remains *** have found that miR-124 starts to be expressed in Xenopus eye and brain from early neural development,and its overexpression leads to malformation of eye and *** present study is to reveal the cellular and molecular mechanisms of its role in early development of Xenopus eye and *** Effects of downregulation and overexpression of miR-124 on neurogenesis and expression of its target candidates in eye and brain were ***-124 precursor molecules or antisense inhibitor and/or mRNAs of miR-124 target candidates was microinjected into one dorsal-animal blastomere at 8-cell stage of Xenopus emebyos for gain-/loss-of functional and rescue *** proliferation in eye and brain was detected by BrdU incorporation and phosphorylated histone-H3.immunohistochemistry *** cells were identified by expression of NCAM and *** expression of neural markers and miR-124 target candidates was checked by RT-PCR,whole-mount in situ hybridization and ***（1） Downregulation of miR-124 decreases cell proliferation and upregulates expression of NCAM and N-tubulin during early neural development;while overexpression of miR-124 gives reverse effects.（2） MiR- 124 negatively regulates expression of candidate target neuroD1,which is expressed complementarily to miR-124 in eye and brain.（3. NeuroD1 overexpression effects in eye mimic that of miR-124 downregulation;and coinjection of neuroD1 with miR-124 can rescue the eye defects caused by overexpression of *** MiR-124 is required for the proper cell proliferation and targets neuroD1 in eye and brain during the early neural development.

关键词： miR-124 cell proliferation neural development neuroDl

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cellular and biochemical control of S-RNase-based self-incompatibility in flowering plants

Cellular and biochemical control of S-RNase-based self-incom...

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第十三届全国植物基因组学大会

Self-incompatibility(SI) is an intra-specific reproductive barrier to prevent selffertilization and to promote cross-fertilization by many flowering *** the SI response,self or genetically related pollen is rejected by the pistil and non-self(cross) pollen accepted for *** outcomes are referred to as self-pollen incompatibility(SPI) and cross-pollen compatibility(CPC),***

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Growth of InAs nanostructures by droplet epitaxy

Growth of InAs nanostructures by droplet epitaxy

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国际纳光电子学研讨会

作者： C. Zhao(1) Y.H. Chen(2) B. Xu(3. Z.G. Wang(4) key laboratory of Semiconductor Materials Science institute of Semiconductors {3. academy of sciences P.O. Box 912 Beijing 100083.China

＜正＞The authors report the growth evolution of InAs dots and rings with the indium deposition amount on GaAs by droplet molecular beam *** is a critical deposition amount to form dots and rings,respectively.

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