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Hab.tat selection b. nestb.x-b.eeding b.rds and Roe Deer are incongruent within a heterogeneous woodland landscape

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Avian Research 2022年第1期13卷 43-48页

作者： kevin b.b.iggs Mark C.Mainwaring Warton CarnforthLancashireLA59RAUK Field Research Station at Fort Missoula Division of Biological SciencesUniversity of MontanaMissoulaMT59812USA

Conserving species relies upon acquiring an understanding of their use of hab.tat,yet our understanding of the use of hab.tat b. co-existing species of different guilds at microgeographic scales remains *** particular,the use of hab.tat b. woodland species is of conservation concern b.cause of widespread declines in woodland *** b.rd declines have b.en ascrib.d,in part,to high deer densities b.cause their b.owsing reduces the availab.lity of nesting sites and *** quantify the microgeographic use of hab.tat b. Roe Deer(Capreolus capreolus)and of Great Tits(Parus major),b.ue Tits(Cyanistes caeruleus)and Pied Flycatchers(Ficedula hypoleuca)in a heterogenous woodland *** examined the use of hab.tat at microgeographic scales b. the deer and the three b.rd species in relation to whether the local hab.tat was flat or wet or had a path,fence or wall within a 25-m radius of 206 randomly selected *** first examined if the occupancy rates of nestb.xes in those locations were correlated with the numb.r of Roe Deer lays and second,examined if the use of hab.tat b. the Roe Deer and the b.rd species were associated with each of the hab.tat features that we *** b.gin b. showing that the use of hab.tat b. Roe Deer is incongruent with the use of hab.tat b. Great Tits,b.ue Tits and Pied Flycatchers during the b.eeding ***,whilst all three b.rd species showed no,or weak,hab.tat preferences,the Roe Deer preferred daytime lay sites that were in flat areas of wet woodland close to paths,whilst there were no significant effects of the presence of fences and *** findings show that the Roe Deer and the three b.rd species differ in the use of hab.tat within a heterogenous woodland landscape,meaning that their use of hab.tat did not overlap at microgeographic ***,the deer showed preferences for flat areas of wet woodland,whilst none of the b.rd species exhib.ted such preferences,and we discuss the implications o

关键词： Capreolus capreolus Cyanistes caeruleus Ficedula hypoleuca Hab.tat use Nestb.xes Nest site selection Parus major Woodlands

Parallel DNA Constrained b.“CC^+Clamps”

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Chemical Research in Chinese Universities 1999年第2期15卷 42-47页

作者： LIU Ke-liang H.Todd Miles Frank b.Howard Joe frazier V.Sasisekharan Lab.ratory of Molecular b.ology NIDDKNational Institutes of HealthBethesdaMaryland20892USA

Parallel DNA helices with reverse WatsonCrick pairing have b.en describ.d in several *** has b.en pointed out,however,that the parallel structures may also b. in equilib.ium with antiparallel WatsonCrick helices due to their closely similar *** avoid the prob.em we have prepared and characterized DNA helices which are constrained to possess parallel polarity b. two dC residues,or CC+clamps,at b.th the ends of AT oligomer *** residues are hemiprotonated b.low neutral pH,forming two stab.e CC+b.se pairs with three hydrogen b.nds at each end of the *** physical properties of these constructs can then b. used to calib.ate those of duplexes having the same sequences b.t lacking the terminal C *** recent studies indicate that dC acid selfstructures are fourstranded with intercalated b.ses,gel electrophoresis indicates that these structures are not present in the molecules describ.d here.

关键词： Parallel DNA helices Reverse Watson-Crick pairing CC+ b.se pair Oligonucleotide helices, Reverse WatsonCrick pairing, CC+ b.se pair, Oligonucleotide

The effects of sun exposure on carotenoid accumulation and oxidative stress in the retina of the House Finch(Haemorhous mexicanus)

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Chinese b.rds 2016年第1期7卷 56-67页

作者： Matthew b.Toomey kevin J.McGraw School of Life Sciences Arizona State University Department of Pathology and Immunology Washington University Medical School

b.ckground: Diet-derived carotenoid pigments are concentrated in the retinas of b.rds and serve a variety of functions, including photoprotection. In domesticated b.rd species(e.g., chickens and quail), retinal carotenoid pigmentation has b.en shown to respond to large manipulations in light exposure and provide protection against photodamage. However, it is not known if or how wild b.rds respond to ecologically relevant variation in sun ***: We manipulated the duration of natural sunlight exposure and dietary carotenoid levels in wild-caught captive House Finches(Haemorhous mexicanus), then measured carotenoid accumulation and oxidative stress in the ***: We found no significant effects of sun exposure on retinal levels of carotenoids or lipid peroxidation, in replicate experiments, in winter(Jan–Mar) and spring/summer(May–June). Dietary carotenoid supplementation in the spring/summer experiment led to significantly higher retinal carotenoid levels, b.t did not affect lipid peroxidation. Carotenoid levels differed significantly b.tween the winter and spring/summer experiments, with higher retinal and lower plasma carotenoid levels in b.rds from the later ***: Our results suggest that variation in the duration of exposure to direct sunlight have limited influence on intraspecific variation in retinal carotenoid accumulation, b.t that accumulation may track other seasonal–environmental cues and physiological processes.

关键词： Carotenoids Haemorhous mexicanus House Finch Photostress Vision

厌氧发酵牛粪对浮萍生长和养分吸收的影响（英文）

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中国生态农业学报（中英文） 2019年第9期27卷 1402-1408页

作者： kevin Kruger b. b.ian He Lide Chen 爱达荷大学农业和生命科学学院土壤和水分系统系爱达荷州83303-1827美国爱达荷大学工程学院生物工程系爱达荷州83844-2060美国

养分从牛粪到农田的循环利用是养牛场可持续发展、资源利用和环境保护的重点研究内容。利用奶牛场废水种植水生植物被认为是一种有效的废水处理及养分循环的方法。本文研究了3种浮萍[少根紫萍0128(Landoltia punctata 0128)、膨胀浮萍75... 详细信息

养分从牛粪到农田的循环利用是养牛场可持续发展、资源利用和环境保护的重点研究内容。利用奶牛场废水种植水生植物被认为是一种有效的废水处理及养分循环的方法。本文研究了3种浮萍[少根紫萍0128(Landoltia punctata 0128)、膨胀浮萍7589(Lemna gib.a 7589)和小浮萍9517(Lemna minuta 9517)]在厌氧发酵过的奶牛场废水中种植时的养分吸收和生物质变化。在28d的测试期间,种植在稀释比例为1∶18的厌氧发酵过的牛奶场废水中的少根紫萍01283获得最高的总氮吸收率(11.6%±1.6%),种植在稀释比例为1∶27厌氧发酵过的牛奶场废水中的少根紫萍0128获得最高的总磷吸收率(15.4%±4.4%);相应地少根紫萍鲜重的增长率分别为0.11 g·d^-1和0.17 g·d^-1。3种浮萍中,少根紫萍最具有吸收牛奶场废水氮、磷并获得较高生物质的潜力。

关键词：牛粪养分循环废水处理厌氧发酵少根紫萍(Landoltia punctata) 膨胀浮萍(Lemna gib.a) 小浮萍(Lemna minuta)

Expanding b.yond canonical metab.lism:Interfacing alternative elements,synthetic b.ology,and metab.lic engineering

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Synthetic and Systems b.otechnology 2018年第1期3卷 20-33页

作者： kevin b.Reed Hal S.Alper McKetta Department of Chemical Engineering The University of Texas at Austin200E Dean Keeton St.Stop C0400AustinTX 78712USA Institute for Cellular and Molecular b.ology The University of Texas at Austin2500 Speedway AvenueAustinTX 78712USA

Metab.lic engineering offers an exquisite capacity to produce new molecules in a renewab.e ***,most industrial applications have focused on only a small sub.et of elements from the periodic tab.e,centered around carb.n *** review aims to illustrate the expanse of chemical elements that can currently(and potentially)b. integrated into useful products using cellular ***,we describ. recent advances in expanding the cellular scope to include the halogens,selenium and the metalloids,and a variety of metal *** examples range from small molecules,heteroatom-linked uncommon elements,and natural products to b.omining and nanotechnology ***,this review covers the promise of an expanded range of elemental incorporations and the future impacts it may have on b.otechnology.

关键词： Metab.lic engineering Synthetic b.ology Unnatural metab.lism Halogenation Cellular factories Non-canonical elements

Geographical location affects size and materials used in the construction of European Pied Flycatcher(Ficedula hypoleuca) nests

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Avian Research 2019年第2期10卷 111-125页

作者： kevin b.b.iggs Lucia E.b.ddle D.Charles Deeming Joseph b.nks Lab.ratories School of Life SciencesUniversity of Lincoln

b.ckground: Nest construction is a key element of avian reproductive b.haviour and the result is often a complex structure that is used for incub.tion of eggs, which represents an extended phenotype. It is known that nest construction is a plastic b.haviour b.t the extent to which plasticity is ob.erved in a single species with a wide geographical distrib.tion is largely unknown. This study sought to b.tter understand variation in nest size and composition across a very wide geographical area. The hypothesis suggested that location would affect size b.t not composition of nests of the European Pied Flycatcher (Ficedula hypoleuca). Methods: Nests and reproductive data were collected from seventeen study sites, spread over 6° of latitude and 3.3° of longitude on the island of Great b.itain. Dimensions of nests were measured b.fore they were deconstructed to determine the masses and types materials used in the outer nest and the cup lining. Results: Geographical variation was ob.erved in b.se thickness of nests b.t not many other dimensions. Nests varied in composition b.t were mainly made of leaf, moss, b.rk, grass, root and fern. Moss was used more to the north and east of the study area compared with more leaf mass towards the south and west. The species of leaf and b.rk used in the nests varied b.tween geographical locations. Additionally, the use of leaves or b.rk from a particular tree species did not reflect the incidence of the tree species in the immediate territory. Conclusions: This study showed that nest composition was affected b. geographical location over a wide area. Variation b.tween nests at each location was high and so it was concluded that differences in nest composition reflect individual selection of materials b.t evidence is such that it remains unclear whether this is delib.rate to fulfil a specific role in the nest, or simply opportunistic with b.rds simply picking up materials with the appropriate characteristics as they find them outside the

关键词： b.eeding success Ficedula hypoleuca Latitude Longitude Nest composition Nest size Nest structure

Interpretation of pipe-jacking and lub.ication records for drives in silty soil

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Underground Space 2020年第3期5卷 199-209页

作者： kevin G.O’Dwyer b.yan A.McCab. b.ian b.Sheil Civil Engineering College of Engineering and InformaticsNational University of IrelandGalwayIreland Department of Engineering Science University of OxfordUnited Kingdom

In recent years,there has b.en an increased resort to microtunnelling/pipe-jacking as a means of constructing underground conduits(for water,sewage,gas,and other utilities)to avoid on-street disruption in urb.n *** this paper,technical details of two 1200 mm internal diameter microtunnels in silty sand totalling over 550 m in length are *** average skin friction values are extremely low for b.th drives suggesting effective lub.ication practice,differences in normalised b.ntonite volumes appear to b. responsib.e for differences in skin *** or near full b.oyancy of the pipeline has b.en demonstrated for the majority of the *** frictional stress increase after a stoppage is shown to depend on not only the stoppage duration b.t also on the normalised lub.ication *** of data in the manner presented in the paper is an important means of assimilating experience of microtunnelling in different ground conditions.

关键词： Microtunnelling Pipe-jacking Skin friction Lub.ication Pipe b.oyancy Stoppages

On Higher Order Pyramidal Finite Elements

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Advances in Applied Mathematics and Mechanics 2011年第2期3卷 131-140页

作者： Liping Liu kevin b.Davies Michal Krızek Li Guan Department of Mathematical Sciences Lakehead University955 Oliver RoadThunder BayOntarioP7B 5E1Canada Institute of Mathematics Academy of SciencesZitna 25CZ-11567 Prague 1Czech Republic College of Computer Science Dongguan University of TechnologyDongguan 523808GuangdongChina

In this paper we first prove a theorem on the nonexistence of pyramidal polynomial b.sis *** we present a new symmetric composite pyramidal finite element which yields a b.tter convergence than the nonsymmetric *** has fourteen degrees of freedom and its b.sis functions are incomplete piecewise triquadratic *** space of ansatz functions contains all quadratic functions on each of four sub.etrahedra that form a given pyramidal element.

关键词： Pyramidal polynomial b.sis functions finite element method composite elements three-dimensional mortar elements.

MINAR1 is a Notch2-b.nding protein that inhib.ts angiogenesis and b.east cancer growth

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Journal of Molecular Cell b.ology 2018年第3期10卷 195-204页

作者： Rachel Xi-Yeen Ho Rosana D. Meyer kevin b. Chandler Esma Ersoy Michael Park Philip A. b.ndzie Nima Rahimi Huihong Xu Catherine E. Costello Nader Rahimi Department of Pathology School of Medicine Boston University Medical Campus Boston MA 02118 USA Department of b.ochemistry and Center for b.omedical Mass Spectrometry School of Medicine Boston University Medical Campus Boston MA 02118 USA

Intrinsically disordered proteins （IDPs）/intrinsicaUy unstructured proteins are characterized b. the lack of fixed or stab.e tertiary structure, and are increasingly recognized as an important class of proteins with major roles in signal transduction and transcriptional regulation. In this study, we report the identification and functional characterization of a previously uncharacterized protein （UPFO258/KIAA1024）, major intrinsically disordered Notch2-associated receptor 1 （MINAR1）. While MINAR1 carries a single transmemb.ane domain and a short cytoplasmic domain, it has a large extraceUular domain that shares no similarity with known protein sequences. Uncharacteristically, MINAR1 is a highly IDP with nearly 70% of its amino acids sequences unstructured. We demonstrate that MINAR1 physically interacts with Notch2 and its b.nding to Notch2 increases its stab.lity and function. MINAR1 is widely expressed in various tissues including the epithelial cells of the b.east and endothelial cells of b.ood vessels. MINAR1 plays a negative role in angioganesis as it inhib.ts angioganesis in cell culture and in mouse matrigal plug and zeb.aflsh anglo- genesis models. Furthermore, while MINAR1 is highly expressed in the normal human b.east, its expression is significantly down- regulated in advanced human b.east cancer and its re-expression in b.east cancer cells inhib.ted tumor growth. Our study demonstrates that MINAR1 is an IDP that negatively regulates angioganesis and growth of b.east cancer cells.

关键词： intrinsically disordered proteins Notch2 angiogenesis b.east cancer MINAR1