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RPLP0/TbP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation

World Journal of Stem Cells 2024年第6期16卷 656-669页

作者： Daniel b Ferreira Leticia M Gasparoni Cristiane F bronzeri Katiucia b S Paiva Laboratory of Extracellular Matrix biology and Cellular Interaction Department of AnatomyInstitute of Biomedical SciencesUniversity of São PauloSão Paulo 05508-000São PauloBrazil

bACKGROUND Validation of the reference gene(RG)stability during experimental analyses is essential for correct quantitative real-time polymerase chain reaction(RT-qPCR)data ***,in an unreliable way,several studies use genes involved in essential cellular functions[glyceraldehyde-3-phosphate dehydro-genase(GAPDH),18S rRNA,andβ-actin]without paying attention to whether they are suitable for such experimental conditions or the reason for choosing such ***,such studies use only one gene when Minimum Information for Publication of Quantitative Real-Time PCR Experiments guidelines recom-mend two or more *** impacts the credibility of these studies and causes dis-tortions in the gene expression *** tissue engineering,the accuracy of gene expression drives the best experimental or therapeutical *** cultivated DPSCs under two conditions:Undifferentiated and osteogenic dif-ferentiation,both for 35 *** evaluated the gene expression of 10 candidates for RGs[ribosomal protein,large,P0(RPLP0),TATA-binding protein(TbP),GAPDH,actin beta(ACTb),tubulin(TUb),aminolevulinic acid synthase 1(ALAS1),tyro-sine 3-monooxygenase/tryptophan 5-monooxygenase activation protein,zeta(YWHAZ),eukaryotic translational elongation factor 1 alpha(EF1a),succinate dehydrogenase complex,subunit A,flavoprotein(SDHA),and beta-2-micro-globulin(b2M)]every 7 d(1,7,14,21,28,and 35 d)by *** data were analysed by the four main algorithms,ΔCt method,geNorm,NormFinder,and bestKeeper and ranked by the RefFinder *** subdivided the samples into eight *** All of the data sets from clonogenic and osteogenic samples were analysed using the RefFinder *** final ranking showed RPLP0/TbP as the two most stable RGs and TUb/b2M as the two least stable *** theΔCt method or NormFinder analysis showed TbP/RPLP0 as the two most stable ***,geNorm analysis showed RPLP0/EF1αin the first *** algorithms’two least stab

关键词： Dental pulp stem cells Reference gene Housekeeping gene Endogenous gene Osteogenic differentiation RefFinder

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Air purifier or fresh air system?A comparison of energy consumption and cost in urban China residential buildings

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Science China(Technological Sciences) 2024年第9期67卷 2637-2647页

作者： GUO Yong SUN ZhiWei WESCHLER Louise b ZHANG YinPing Department of building Science Tsinghua UniversityBeijing 100084China beijing Key Laboratory of Indoor Air Quality Evaluation and Control Beijing 100084China Independent Researcher Colts Neck 07722NJUSA

Chinese urban residential buildings face serious indoor air quality *** purification systems(e.g.,air purifiers,fresh air systems)that supply fresh air or purify indoor air are widely used in residential buildings to improve indoor air *** the challenges of energy conservation and emission reduction,it is urgent to guide residents on choosing appropriate air purification *** key problem is how to identify the best air purification system with the lowest energy consumption or operating *** of air purification systems have rarely been conducted on the premise that they should deliver air such that the main indoor pollutant concentrations all meet air quality *** ignored in comparisons are the increased filtration resistance caused by dust accumulation on the filter and the cost of filter *** this study,we propose a method to compare residential air purification systems based on energy consumption and total cost,and we provide methods to select air purification systems in China's different climatic *** used a standard two-person bedroom as the example,and determined the most suitable(minimum cost or energy consumption)air purification systems for the capital cities of the provinces in Chinese mainland.

关键词： IAQ fresh air system air purifier energy consumption cost

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Function and dysfunction of GEMIN5:understanding a novel neurodevelopmental disorder

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Neural Regeneration Research 2024年第11期19卷 2377-2386页

作者： Charles H.Nelson Udai b.Pandey Department of Pediatrics Division of Child NeurologyChildren’s Hospital of PittsburghUniversity of Pittsburgh Medical CenterPittsburghPAUSA Children’s Neuroscience Institute Children’s Hospital of PittsburghUniversity of Pittsburgh Medical CenterPittsburghPAUSA

The recent identification of a neurodevelopmental disorder with cerebellar atrophy and motor dysfunction(NEDCAM)has resulted in an increased interest in GEMIN5,a multifunction RNA-binding *** the largest member of the survival motor neuron complex,GEMIN5 plays a key role in the biogenesis of small nuclear ribonucleoproteins while also exhibiting translational regulatory functions as an independent *** many questions remain regarding both the pathogenesis and pathophysiology of this new disorder,considerable progress has been made in the brief time since its *** this review,we examine GEMIN5 within the context of NEDCAM,focusing on the structure,function,and expression of the protein specifically in regard to the disorder ***,we explore the current animal models of NEDCAM,as well as potential molecular pathways for treatment and future directions of *** review provides a comprehensive overview of recent advances in our understanding of this unique member of the survival motor neuron complex.

关键词： cerebellar atrophy GEMIN5 neurodevelopmental disorder neurological disease SMN complex

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Astrophysical S-factor and reaction rate for 15N(p,γ)16O within the modified potential cluster model

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Chinese Physics C 2024年第4期48卷 130-145页

作者： S.b.Dubovichenko A.S.Tkachenko R.Ya.Kezerashvili N.A.burkova b.M.Yeleusheva Fesenkov Astrophysical Institute 050020 AlmatyKazakhstan New York City College of Technology City University of New YorkBrooklyn11201 New YorkUSA Graduate School and University Center City University of New York10016 New YorkUSA al-Farabi Kazakh National University 050040 AlmatyKazakhstan

We study radiative p^(15)N capture on the ground state of ^(16)O at stellar energies within the framework of a modified potential cluster model(MPCM)with forbidden states,including low-lying *** investigation of the ^(15)N(p,γ0)^(16)O reaction includes the consideration of ^(3)S_(1) resonances due to E1 transitions and the contribution of the ^(3)P_(1) scattering wave in the p+^(15)N channel due to the ^(3)P_(1)→^(3)P_(0)M1 *** calculated the astrophysical low-energy S-factor,and the extrapolated S(0)turned out to be within 34.7−40.4 keV·*** important role of the asymptotic constant(AC)for the ^(15)N(p,γ0)16O process with interfering ^(3)S_(1)(312)and ^(3)S_(1)(962)resonances is elucidated.A comparison of our calculation for the S-factor with existing experimental and theoretical data is addressed,and a reasonable agreement is *** reaction rate is calculated and compared with the existing *** has negligible dependence on the variation of AC but shows a strong impact of the interference of ^(3)S_(1)(312)and ^(3)S_(1)(962)resonances in reference to the CNO Gamow windows,especially at low *** estimate the contribution of cascade transitions to the reaction rate based on the exclusive experimental data from ***.C.85,065810(2012).The reaction rate enhancement due to the cascade transitions is observed from T_(9)>0.3 and reaches the maximum factor~1.3 at T_(9)=*** present the Gamow energy window and a comparison of rates for radiative proton capture reactions ^(12)N(p,γ)^(13)O,^(13)N(p,γ)^(14)O,^(14)N(p,γ)^(15)O,and ^(15)N(p,γ)^(16)O obtained in the framework of the MPCM and provide the temperature windows,prevalence,and significance of each process.

关键词： low and astrophysical energies p^(15)N system thermonuclear reaction rate potential cluster model CNO-cycle

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The rate of deadwood decomposition processes in tree stand gaps resulting from bark beetle infestation spots in mountain forests

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Forest Ecosystems 2024年第3期11卷 339-346页

作者： Ewa błonska Adam Gorski Jarosław Lasota Department of Ecology and Silviculture Faculty of ForestryUniversity of Agriculture in Krakow29 Listopada 46 Str.31-425KrakowPoland

Decaying wood is an essential element of forest ecosystems and it affects its other *** aim of our research was to determine the decomposition rate of deadwood in various humidity and thermal conditions in the gaps formed in the montane forest *** research was carried out in the babiog orski National *** research plots were marked out in the gaps of the stands,which were formed as a result of bark beetle *** plots were located in undisturbed *** research covered wood of two species–spruce and beech in the form of cubes with dimensions of 50 mm×50 mm×22 *** samples were placed directly on the soil surface and subjected to laboratory analysis after 36 months.A significant influence of the wood species and the study plot type on the physicochemical properties of the tested wood samples was *** characteristics strongly correlated with soil moisture.A significantly higher mass decline of wood samples was recorded on the reference study plots,which were characterized by more stable moisture *** decomposition of wood in the gaps regardless of the species is related to lower *** wood species covered by the study differed in the decomposition *** wood samples were characterized by a significantly higher decomposition rate compared to beech wood *** research has confirmed that disturbances that lead to the formation of gaps have a direct impact on the decomposition process of deadwood.

关键词： bark beetle beech Decomposition rate Forest ecosystem Mass decline Spruce Woody debris

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brassinosteroid biosynthesis and signaling:Conserved and diversified functions of core genes across multiple plant species

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Plant Communications 2024年第9期5卷 72-100页

作者： brian Zebosi Erik Vollbrecht Norman b.best Department of Genetics Development and Cell BiologyIowa State UniversityAmesIA 50011USA Interdepartmental Genetics and Genomics Graduate Program Iowa State UniversityAmesIA 50011USA USDA-ARS Plant Genetics Research UnitColumbiaMO 65201USA

brassinosteroids(bRs)are important regulators that control myriad aspects of plant growth and development,including biotic and abiotic stress responses,such that modulating bR homeostasis and signaling presents abundant opportunities for plant breeding and crop *** and other proteins involved in the biosynthesis and signaling of bRs are well understood from molecular genetics and phenotypic analysis in Arabidopsis thaliana;however,knowledge of the molecular functions of these genes in other plant species,especially cereal crop plants,is *** this manuscript,we comprehensively review functional studies of bR genes in Arabidopsis,maize,rice,Setaria,brachypodium,and soybean to identify conserved and diversified functions across plant species and to highlight cases for which additional research is in *** performed phylogenetic analysis of gene families involved in the biosynthesis and signaling of bRs and re-analyzed publicly available transcriptomic *** trees coupled with expression data provide a valuable guide to supplement future research on bRs in these important crop species,enabling researchers to identify gene-editing targets for bR-related functional studies.

关键词： brassinosteroids maize rice Setaria soybean brachypodium Arabidopsis

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Compressive property and energy absorption characteristic of interconnected porous Mg-Zn-Y alloys with adjusting Y addition

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Journal of Magnesium and Alloys 2024年第1期12卷 171-185页

作者： J.A.Liu S.J.Liu b.Wang W.b.Sun X.J.Liu Z.W.Han College of Materials Science and Engineering Key Laboratory of Automobile Materials(Ministry of Education)Jilin UniversityChangchun 130022PR China Key Laboratory of bionic Engineering(Ministry of Education) College of Biological and Agricultural EngineeringJilin UniversityChangchun130022PR China Weihai Institute for bionics-Jilin University Weihai 264205PR China

In this study,interconnected porous Mg-2Zn-xY alloys with different phase compositions were prepared by various Y additions(x=0.4,3,and 6 wt.%)to adjust the compressive properties and energy absorption *** characterization methods were then applied to identify the microstructure of the porous Mg-Zn-Y and describe the details of the second *** tests were performed at room temperature(RT),200℃,and 300℃to study the impact of the Y addition and testing temperature on the compressive properties of the porous *** experimental results showed that a high Y content promotes a microstructure refinement and increases the volume fraction of the second *** the Y content increases,different Mg-Zn-Y ternary phases appear:I-phase(Mg_(3)Zn_(6)Y),W-phase(Mg_(3)Zn_(3)Y_(2)),and LPSO phase(Mg_(12)ZnY).When the Y content ranges between 0.4%and 6%,the compressive strength increases from 6.30MPa to 9.23 MPa,and the energy absorption capacity increases from 7.33 MJ/m^(3)to 10.97 MJ/m^(3)at RT,which is mainly attributed to the phase composition and volume fraction of the second ***,the average energy absorption efficiency is independent of the Y *** addition,the compressive deformation behaviors of the porous Mg-Zn-Y are altered by the testing *** compressive strength and energy absorption capacity of the porous Mg-Zn-Y decrease due to the softening effect of the high temperature on the *** deformation behaviors at different temperatures are finally observed to reflect the failure mechanisms of the struts.

关键词： Porous magnesium Rare earth elements Microstructure Compressive behavior Energy absorption characteristic

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Iron regulatory protein 1: the deadly switch of ferroptosis

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Neural Regeneration Research 2024年第3期19卷 477-478页

作者： Daniel A.bórquez Pamela J.Urrutia Center for biomedical Research Faculty of MedicineUniversidad Diego PortalesSantiagoChile Institute of Nutrition&Food Technology(INTA) Universidad de ChileSantiagoChile Geroscience Center for brain Health and Metabolism SantiagoChile

Ferroptosis,an iron-dependent cell death:Ferroptosis is a type of regulated necrosis,characterized by redox-active iron accumulation and increased free radical production derived by Fenton chemistry,that triggers oxidation of polyunsaturated fatty acids in phospholipids,loss of cellular membranes integrity,and leakage of intracellular contents.

关键词： integrity switch Iron

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Radiative capture of proton through the ^(14)N(p,γ)^(15)O reaction at low energy

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Chinese Physics C 2024年第4期48卷 183-192页

作者： b.F.Irgaziev Abdul Kabir Jameel-Un Nabi National University of Uzbekistan Tashkent 100174Uzbekistan Space and Astrophysics Research lab National Centre of GIS and Space ApplicationsDepartment of Space ScienceInstitute of Space TechnologyIslamabad 44000Pakistan University of Wah Quaid AvenueWah Cantt 47040PunjabPakistan

The CNO cycle is the main source of energy in stars more massive than our *** process defines the energy production,the duration of which can be used to determine the lifetime of massive *** cycle is an important tool for determining the age of globular *** proton capture via p+^(14)N→^(15)O+γ,at energies of astrophysical interest,is an important process in the CNO *** this project,we apply a potential model to describe both non-resonant and resonant reactions in the channels where radiative capture occurs through electric E1 *** employed the R-matrix method to describe the ongoing reactions via M1 resonant transitions,when it was not possible to correctly reproduce the experimental data using the potential *** partial components of the astrophysical S-factor are calculated for all possible electric and magnetic dipole transitions in ^(15)*** linear extrapolated S-factor at zero energy(S(0))agrees well with earlier reported values for all transition types considered in this *** on the value of the total astrophysical S-factor,depending on the collision energy,we calculate the nuclear reaction rates for p+^(14)N→^(15)O+γ.The computed rates agree well with the results reported in the NACRE II Collaboration and most recent existing measurements.

关键词： CNO cycle potential model R-matrix method cross-section astrophysical S-factor nuclear rates.

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Probing the bardeen-Kiselev black hole with the cosmological constant caused by Einstein equations coupled with nonlinear electrodynamics using quasinormal modes and greybody bounds

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Communications in Theoretical Physics 2024年第6期76卷 102-119页

作者： S R Wu b Q Wang Z W Long College of Physics Guizhou UniversityGuiyang 550025China College Pharmacy Guizhou University of Traditional Chinese MedicineGuiyang 550025China

In this work,we investigate a static and spherically symmetric bardeen-Kiselev black hole(bH)with the cosmological constant,which is a solution of the Einstein-non-linear Maxwell field *** compute the quasinormal frequencies for the bardeen-Kiselev bH with the cosmological constant due to electromagnetic and gravitational *** varying the bH parameters,we discuss the behavior of both real and imaginary parts of the bH quasinormal frequencies and compare these frequencies with the Reissner-Nordström-de Sitter bH surrounded by quintessence(RN-dSQ).Interestingly,it is shown that the responses of the bardeen-Kiselev bH with the cosmological constant and the RN-dSQ under electromagnetic perturbations are different when the charge parameter q,the state parameter w and the normalization factor c are varied;however,for the gravitational perturbations,the responses of the bardeen-Kiselev bH with the cosmological constant and the RN-dSQ are different only when the charge parameter q is ***,compared with the gravitational perturbations,the electromagnetic perturbations can be used to understand nonlinear and linear electromagnetic fields in curved spacetime *** interesting observation is that,due to the presence of Kiselev quintessence,the electromagnetic perturbations around the bardeen-Kiselev bH with the cosmological constant damps faster and oscillates slowly;for the gravitational perturbations,the quasinormal mode decays slowly and oscillates *** also study the reflection and transmission coefficients along with the absorption cross section in the bardeen-Kiselev bH with the cosmological constant;it is shown that the transmission coefficients will increase due to the presence of Kiselev quintessence.

关键词： quasinormal modes greybody factors bardeen-Kiselev black hole with cosmological constant

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