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聚合酶链反应检测乙型肝炎患者肝外组织中的乙型肝炎病毒DNA

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中华内科杂志 1994年第4期33卷 232-236页

作者：郎振为闫惠平黄德在王健吴和 Zhang JL Perry A Byrne SK stiver GH 北京佑安医院北京市肝炎研究所加拿大不列颠哥伦比亚省疾病控制中心

应用巢式聚合酶链（ＰＣＲ）技术对１８例石蜡包埋的胆囊、肾、脾、肾上腺、心、睾丸、胰腺及肝脏组织的乙型肝炎病毒（ＨＢＶ）ＤＮＡ进行了检测，并与其免疫组化及原位杂交方法作比较。二组引物检测的结果表明：肝组织中均有ＨＢＶ感... 详细信息

应用巢式聚合酶链（ＰＣＲ）技术对１８例石蜡包埋的胆囊、肾、脾、肾上腺、心、睾丸、胰腺及肝脏组织的乙型肝炎病毒（ＨＢＶ）ＤＮＡ进行了检测，并与其免疫组化及原位杂交方法作比较。二组引物检测的结果表明：肝组织中均有ＨＢＶ感染，其中５例有ＨＢＶ的复制。肝外组织中ＨＢＶ检出率分别为胆囊８５．７％、７５．０％、肾７２．７％、肾上腺６６．７％、心脏５５．６％、睾丸５５．６％和胰腺５４．５％，但均未检测出ＨＢＶ的复制，ＰＣＲ检测发现与免疫组化及原位杂交结果基本一致。ＨＢＶ可以感染肝外组织但并不在这些组织中复制的发现可以解释受感染的肝外组织可以作为肝外感染源，但并不引起这些脏器出现明显的病理变化。

关键词：聚合酶链反应乙型肝炎病毒 DNA

聚合酶链反应检测乙型肝炎患者心肌组织中 HBV DNA

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中华传染病杂志 1994年第1期12卷 9-13页

作者：郎振为阎惠平李俊强 Zhang JL Perry A Byrne SK stiver GH 北京佑安医院北京市肝炎研究所加拿大不列颠哥伦比亚省疾病控制中心应用研究与开发实验室加拿大温哥华总医院

应用巢式聚台酶链反应（ＰＣＲ）技术检测了１８例乙型肝炎（乙肝）患者石蜡包埋心、肝组织中的ＨＢＶＤＮＡ，并与其免疫组化及原位杂交结果作了比较。二组引物检测的结果表明：在肝组织中均有ＨＢＶ感染，其中５例有ＨＢＶ复制。心肌... 详细信息

应用巢式聚台酶链反应（ＰＣＲ）技术检测了１８例乙型肝炎（乙肝）患者石蜡包埋心、肝组织中的ＨＢＶＤＮＡ，并与其免疫组化及原位杂交结果作了比较。二组引物检测的结果表明：在肝组织中均有ＨＢＶ感染，其中５例有ＨＢＶ复制。心肌组织ＨＢＶＤＮＡ的检出率为５５．６％，不存在ＨＢＶ的复制。心脏病理检查可见一些非特异性改变，如脂变、水肿和纤维素样坏死等。病理变化与心电图、临床症状、体征及ＰＣＲ检测结果无相关件。以上结果提示：乙肝患者心脏出现的一些病变可能是由于沉积于心肌内的免疫复合物介导损伤的结果。

关键词：聚合酶链反应乙型肝炎病毒 DNA

聚合酶链反应检测乙型肝炎患者肾组织中的HBVDNA

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中华病理学杂志 1994年第3期23卷 144-147页

作者：郎振为阎惠平黄德庄 Zhang JL Perry A ByrneSK stiver GH 吴和北京佑安医院病理科北京市肝炎研究所加拿大不列颠哥伦比亚省疾病控制中心应用研究与开发实验室

应用巢式聚合酶链反应（ＰＣＲ）技术对１１例乙型肝炎患者石蜡包埋的肾、肝组织中的ＨＢＶＤＮＡ进行了检测，并与其免疫组化及原位杂交结果作了比较。二组引物检测的结果表明：在肝组织中均有ＨＢＶ的感染，其中５例存有ＨＢＶ的复制... 详细信息

应用巢式聚合酶链反应（ＰＣＲ）技术对１１例乙型肝炎患者石蜡包埋的肾、肝组织中的ＨＢＶＤＮＡ进行了检测，并与其免疫组化及原位杂交结果作了比较。二组引物检测的结果表明：在肝组织中均有ＨＢＶ的感染，其中５例存有ＨＢＶ的复制；倚组织中ＨＢＶＤＮＡ的检出率为８／１１（７２．７％），未检测出有ＨＢＶ的复制，包括２例有膜性肾炎和膜增生性肾炎病变的病例。ＨＢＶ可以感染肾组织但并不在肾组织中复制。这一实验结果较为支持乙型肝炎患者肾脏出现的病变可能系沉积于肾小球内的免疫复合物介导损伤的结果。

关键词：聚合酶链反应乙型肝炎 DNA 肾

Design of N-11-Azaartemisinins Potentially Active against Plasmodium falciparum by Combined Molecular Electrostatic Potential, Ligand-Receptor Interaction and Models Built with Supervised Machine Learning Methods

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Journal of Biophysical Chemistry 2023年第1期14卷 1-29页

作者： Jeferson stiver Oliveira de Castro José Ciríaco Pinheiro Sílvia Simone dos Santos de Morais Heriberto Rodrigues Bitencourt Antonio Florêncio de Figueiredo Marcos Antonio Barros dos Santos Fábio dos Santos Gil Ana Cecília Barbosa Pinheiro Instituto de Educação Ciência e Tecnologia do Pará Castanhal PA Brasil Laboratório de Química Teórica e Computacional Universidade Federal do Pará Belém PA Brasil Instituto Amazônia dos Saberes São Luís MA Brasil Universidade do Estado do Amapá Macapá AP Brasil Laboratório de Síntese Universidade Federal do Pará Belém PA Brasil Universidade do Estado do Pará Belém PA Brasil Fundação Santa Casa de Misericórdia do Pará Belém PA Brasil

N-11-azaartemisinins potentially active against Plasmodium falciparum are designed by combining molecular electrostatic potential (MEP), ligand-receptor interaction, and models built with supervised machine learning methods (PCA, HCA, KNN, SIMCA, and SDA). The optimization of molecular structures was performed using the B3LYP/6-31G* approach. MEP maps and ligand-receptor interactions were used to investigate key structural features required for biological activities and likely interactions between N-11-azaartemisinins and heme, respectively. The supervised machine learning methods allowed the separation of the investigated compounds into two classes: cha and cla, with the properties εLUMO+1 (one level above lowest unoccupied molecular orbital energy), d(C6-C5) (distance between C6 and C5 atoms in ligands), and TSA (total surface area) responsible for the classification. The insights extracted from the investigation developed and the chemical intuition enabled the design of sixteen new N-11-azaartemisinins (prediction set), moreover, models built with supervised machine learning methods were applied to this prediction set. The result of this application showed twelve new promising N-11-azaartemisinins for synthesis and biological evaluation.

关键词： Antimalarial Design MEP Ligand-Receptor Interaction Supervised Machine Learning Methods Models Built with Supervised Machine Learning Methods

Designing Artemisinins with Antimalarial Potential, Combining Molecular Electrostatic Potential, Ligand-Heme Interaction and Multivariate Models

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Computational Chemistry 2023年第1期11卷 1-23页

作者： Josué de Jesus Oliveira Araújo Ricardo Morais de Miranda Jeferson stiver Oliveira de Castro Antonio Florêncio de Figueiredo Ana Cecília Barbosa Pinheiro Sílvia Simone dos Santos Morais Marcos Antonio Barros dos Santos Andréia de Lourdes Ribeiro Pinheiro Andréia de Lourdes Ribeiro Pinheiro Fábio dos Santos Gil Heriberto Rodrigues Bitencourt Gustavo Nery Ramos Alves José Ciríaco Pinheiro Laboratório de Química Teórica e Computacional Universidade Federal do Pará Belém PA Brasil Instituto Federal de Educaç ã o Ciência e Tecnologia do Pará Belém PA Brasil Universidade do Estado do Amapá Macapá AP Brasil Fundaç ã o Santa Casa de Misericórdia do Pará Belém PA Brasil Universidade do Estado do Pará Barcarena PA Brasil Departamento de Biologia Universidade do Estado do Maranh&atilde o S&atilde o Luís MA Brasil Laboratório de Síntese Organica Universidade Federal do Pará Belém PA Brasil

Artemisinins tested against W-2 strains of malaria falciparum are investigated with molecular electrostatic potential (MEP), in an attempt to identify key features of the compounds that are necessary for their activities, as well as to investigate likely interactions with the receptor in a biological process and to use that information to propose new molecules. In order to discover the best geometry involving the ligand-receptor complexes (heme) studied and help in the proposition of the new derivatives, molecular simulations of interactions between the most negative charged region around the peroxide and heme locates (the ones around the Fe2+ ion) were carried out. In addition, PCA (principal components analysis), HCA (hierarchical cluster analysis), SDA (stepwise discriminant analysis), and KNN (K-nearest neighbor) multivariate models were employed to investigate which descriptors are responsible for the classification between the higher and lower antimalarial activity of the compounds, and also this information was used to propose new potentially active molecules. The information accumulated in studies of MEP, molecular docking, and multivariate analysis supported the proposal of new structures with potential antimalarial activities. The multivariate models constructed were applied to the new structures and indicated numbers 19 and 20 as the most prominent for syntheses and biological assays.

关键词： Artemisinins Antimalarial Potential Molecular Electrostatic Potential Ligand-Heme Interaction Multivariate Models

良性颞叶前部硬膜外血肿:头部钝伤后无痛性病变伴特征性CT表现

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国际医学放射学杂志 2010年第6期 577-577页

作者： A.D.Gean N.J.Fischbein D.D.Purcell A.H.Aiken G.T.Manley S.I.stiver 葛传彪 Department of Radiology University of CaliforniaSan FranciscoSan FranciscoCalif Department of Radiology Stanford University Medical CenterStanfordCalif

目的研究头部钝伤所致硬膜外血肿(EDH)的独特亚型的发病率、发病机制、影像特征和临床的重要性。材料与方法该项研究经医院伦理审查委员会评审与批准,符合

关键词：硬膜外血肿脑出血钝伤颅内损伤颞叶大脑皮质头部解剖部位 CT