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Niche Differentiation of Phenol-Degrading Microorganisms in UASB Granular Sludge as Revealed by Fluorescence in situ Hybridization

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Engineering 2022年第2期8卷 61-66页

作者： Kengo kubota kei Igarashi Masayoshi Yamada Yasuyuki Takemura Yu-You Li Hideki Harada Department of Civil and Environmental Engineering Tohoku UniversitySendai 980-8579Japan Department of Urban Environmental Design and Engineering National Institute of TechnologyKagoshima CollegeKirishima 899-5193Japan New Industry Creation Hatchery Center Tohoku UniversitySendai 980-8579Japan

A microbial community structure of granules harvested from an anaerobic sludge blanket reactor treating phenolic wastewater was investigated using fluorescence in situ hybridization(FISH)and clone library *** of Syntrophorhabdaceae and Cryptanaerobacter were observed to be responsible for phenol *** accurate taxonomic assignment of Cryptanaerobacter clones,phylogenetic analysis using nearly full-length 16S ribosomal RNA(rRNA)gene sequences was *** oligonucleotide probes were designed to detect the following three taxonomic groups:Syntrophorhabdaceae,Cryptanaerobacter,and *** analysis of thin sections of anaerobic granules showed a random distribution of bacteria and ***,a well-defined distribution of Syntrophorhabdaceae,Cryptanaerobacter,and Syntrophus was *** and Syntrophus were found on the outer layer of the granules and were closely associated with each other,while Syntrophorhabdaceae was located in the deeper part of the *** specific distribution of the bacteria is most likely due to their metabolic association and affinity for the *** degradation in the granular sludge was observed to be carried out in the following ***,Cryptanaerobacter converts phenol to benzoate,which is then degraded by Syntrophus into *** syntrophic degradation of phenol occurs near the surface of the granule,where the phenol concen-tration is *** the deeper part of the granule,where the phenol concentration is lower,Syntrophorhabdaceae degrades phenol into *** observed that Syntrophorhabdaceae is less likely to produce benzoate as an intermediate to feed the neighboring organisms,which contradicts the theo-ries presented by previous studies.

关键词： Cryptanaerobacter Fluorescence in situ hybridization Anaerobic phenol degradation Syntrophorhabdaceae Syntrophus UASB granular sludge

Synthesis of Capsaicin Oligosaccharides and Their Anti-Allergic Activity<br>——Synthesis of Capsaicin Oligosaccharides as Anti-Allergic Food-Additives

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Advances in Chemical Engineering and Science 2012年第1期2卷 45-49页

作者： kei Shimoda Naoji kubota Masaaki Akagi 不详

The production of β-maltooligosaccharides of capsaicin was investigated using Lactobacillus delbrueckii and cyclodextrin glucanotransferase (CGTase) as biocatalysts. The cells of L. delbrueckii glucosylated capsaicin to give its β-glucoside. The β-glucoside of capsaicin was converted into the corresponding β-maltoside and β-maltotrioside by CGTase. On the other hand, β-melibioside and β-isomaltoside of capsaicin, which were two new compounds, were synthesized by chemical glycosylation. The β-glucoside, β-maltoside, β-melibioside, and β-isomaltoside of capsaicin showed inhibitory effects on IgE antibody production.

关键词： Glycosylation Capsaicin Lactobacillus Delbrueckii CGTase Anti-Allergic Activity

Usefulness of Y-shaped sheaths in CT angiography for examination of liver tumors

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World Journal of Gastroenterology 2010年第37期16卷 4704-4708页

作者： Toru Ishikawa Kazuo Higuchi Tomoyuki kubota kei-ichi Seki Terasu Honma Toshiaki Yoshida Takeo Nemoto keiko Takeda Tomoteru Kamimura Department of Gastroenterology and Hepatology Saiseikai Niigata Second Hospital Department of Radiology Saiseikai Niigata Second Hospital

AIM: To conduct a single-stage, combined computed tomography (CT) arterial portography (CTAP) and CT arteriography (CTA) imaging operation, we used Y-shaped sheaths with 2 valves, which allowed the insertion of 2 catheters simultaneously. METHODS: Of 1254 patients who underwent abdominal angiography for transarterial embolization and/or intraarterial chemotherapy in our department from May 2002 to November 2009, 664 patients in whom Y-shaped sheaths with 2 valves were used underwent CT angiography using a combination of CTA and CTAP. The Seldinger method was used to insert a 10 cm Y-shaped short sheath with 2 valves into the femoral artery. Under radiographic guidance, a 3.2 French (Fr) catheter was placed in the celiac artery or proper hepatic artery, and a second 3.2 Fr catheter was then placed distal to the inferior pancreaticoduodenal artery of the superior mesenteric artery. CTAP was then performed followed by CTA 10 min later. Photographs were taken during the early and late phases of the ***: Insertion of 3.2 Fr catheters was not possible in 6 of 664 (0.9%) patients with strong curvature of the femoral artery and 4 of 664 (0.6%) patients with strong curvature of the abdominal aorta. In addition, performing CTAP and CTA as a single-stage combined intervention was not possible in 14 of 664 (2.1%) patients whose right hepatic artery originated from the superior mesenteric artery and in 8 of 664 (1.2%) patients whose left hepatic artery branched from the left gastric artery. There were no sheath-related complications such as those related to arterial dissection or hemostasis. CONCLUSION: Although transfers to and from the CT room were necessary for anatomically variant patients, CT angiography using the Y-shaped sheath for combined CTAP and CTA was considered useful.

关键词： Computed tomography angiography Interventional radiology Liver tumors

Efficacy of 5-Fluorouracil and High-Concentration Cisplatin Suspended in Lipiodol by Short-Term Hepatic Arterial Infusion Chemotherapy for Advanced Hepatocellular Carcinoma with Portal Vein Tumor Thrombosis

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Journal of Cancer Therapy 2015年第13期6卷 1151-1161页

作者： Yutaka Yata Masashi Namikawa Tatsuya Ohyama Takashi Ohsaki Daisuke Kanda Takeshi Hatanaka kei Shibuya Jun kubota Hitoshi Takagi Terumi Takahara Teruo Yoshinaga Department of Gastroenterology Saiseikai Maebashi Hospital Maebashi-City Japan Department of Radiology Saiseikai Maebashi Hospital Maebashi-City Japan Third Department of Internal Medicine Toyama University Toyama-City Japan

Background: Since advanced hepatocellular carcinoma (HCC) is potentially fatal, and patients’ quality of life (QOL) often deteriorates during their treatment, improving the prognosis and QOL of patients given chemotherapy is very important. In addition, cost-effective treatments are highly desirable when chemotherapy must be given repeatedly. The aim of this study was to evaluate the efficacy and usefulness of 5-fluorouracil (5-FU) and high-concentration cisplatin by short-term hepatic arterial infusion chemotherapy (3-day FPL) in advanced HCC patients. Methods: Thirty patients with unresectable advanced HCC were enrolled. The patients underwent hepatic arterial infusion chemotherapy via the implanted port system with 5-FU on days 1 - 3 and a fine-powder formulation of cisplatin in suspended pre-warmed lipiodol on day 2 every 4 to 10 weeks. Tumor response was assessed one month later with CT. Results: All patients had evidence of portal vein invasion (Vp2-4). Four patients achieved a complete response (CR), 8 patients achieved a partial response (PR), and 7 patients had stable disease (SD). The median progression-free survival (PFS) and overall survival (OS) were 198 days and 452 days, respectively. The OS was significantly longer in the successful disease control group (CR, PR, and SD) than in the progressive disease group (P < 0.005). Conclusions: Three-day FPL was effective and tolerable in advanced HCC patients due to its shorter time of administration than conventional FP therapy. Therefore, repetitive 3-day FPL appears useful and contributes to improving the prognosis and QOL of patients with advanced HCC. In addition, this protocol is a cost-effective treatment.

关键词： Advanced Hepatocellular Carcinoma (HCC) Portal Vein Tumor Thrombosis (PVTT) Hepatic Arterial Infusion Chemotherapy (HAIC) 5-FU a Fine-Powder Formulation of Cisplatin Quality of Life (QOL) Cost-Effective Treatment