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THE ROLES OF REACTION INHOMOGENEITY IN PHASE SEPARATION KINETICS AND MORPHOLOGY OF REACTIVE POLYMER BLENDS

Chinese Journal of Polymer Science 2009年第1期27卷 23-36页

作者： Qui Tran-Cong-Miyata Dan-Thuy Van-Pham kei noma Tomohisa Norisuye Hideyuki Nakanishi Department of Macromolecular Science and Engineering Graduate School of Science and Technology Kyoto Institute of TechnologyMatsugasakiKyoto 606-8585Japan

The roles of reaction inhomogeneity in phase separation of polymer mixtures were described and summarized via two examples:photocross-link of polymer mixtures in the bulk state and photopolymerization of monomer in the liquid state. The reaction kinetics,the reaction-induced elastic strain and the phase separation kinetics were monitored respectively by UV-Vis spectroscopy,Mach-Zehnder interferometry and laser-scanning confocal *** was found that phase separation in the bulk state was strongly inf...

关键词： Photo-crosslink reaction Phase separation Reaction-induced deformation Mach-Zehnder interferometry Laserscanning confocal microscopy

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Effect of Low-pressure Carbonation on the Heat Inactivation and Cytoplasmic Acidification of Saccharomyces cerevisiae

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Journal of Food Science and Engineering 2012年第12期2卷 712-718页

作者： Wannaporn Klangpetch Tomoe Nakail kei Nishiyama Seiji noma Noriyuki Igura Mitsuya Shimoda Graduate School of Bioresource and Bioenvironmental Sciences Kyushu University Fukuoka Japan Division of Bioresources and Bioenvironmental Sciences Kyushu University Fukuoka Japan

Effect of low-pressure carbonation （LPC） on heat inactivation of Saccharomyces cerevisiae was investigated. The cell suspension was carbonated at 1 MPa and 4℃ for 15 min and subsequently heated from 51 to 61 ℃ and 5 s to 5 min （heating with LPC）. As a control experiment, cell suspension was heat-treated under atmospheric pressure without LPC （heating）. The inactivation ratio of heating at 53℃ and 55℃ for l rain with LPC was approximately 1 log order higher than heating alone. Extending heating time to 5 min did not widen the difference in the inactivation ratio between heating with LPC and heating alone at both heating temperatures. At 57℃, the difference in inactivation ratio increased from 1 to 2.5 log order with extending treatment time from 5 to 15 s. The results suggested that the enhanced inactivation effect by LPC was obtained at the higher temperature with short time treatment than the lower temperature with longer time treatment. Under fluorescence microscope observation of LPC-treated cell stained with LysoSensor probe, it seemed that LPC was hardly able to acidify the cytoplasm ofS. cerevisiae. It is considered that the ability orS. cerevisiae ceils to keep their cytoplasmic pH during LPC resulted in the inferior increase in heat inactivation ratio by LPC as compared with bacteria in the previous studies.

关键词： Saccharomyces cerevisiae low-pressure carbonation heat inactivation heat sensitivity.

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