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Adventitious Roots and Secondary Metabolism

生物工程学报 2008年第5期24卷 711-716页

作者： Hosakatte Niranjana Murthy Eun Joo Hahn kee yoeup paek Research Center for the Development of Advanced Horticultural Technology Chungbuk National University Cheongju 361-763 South Korea Department of Botany Karnatak University Dharwad 580003 India

Plants are a rich source of valuable secondary metabolites and in the recent years plant cell, tissue and organ cultures have been developed as an important alternative sources for the production of these compounds. Adventitious roots have been successfully induced in many plant species and cultured for the production of high value secondary metabolites of pharmaceutical, nutraceutical and industrial importance. Adoption of elicitation methods have shown improved synthesis of secondary metabolites in adventitious root cultures. Development of large-scale culture methods using bioreactors has opened up feasibilities of production of secondary metabolites at the industrial levels. In the present review we summarize the progress made in recent past in the area of adventitious root cultures for the production of secondary metabolites.

关键词：次生代谢植物细胞基因

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Effects of tissue-cultured mountain ginseng （Panax ginseng CA Meyer） extract on male patients with erectile dysfunction

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Asian Journal of Andrology 2009年第3期11卷 356-361页

作者： Tae-Hwan Kim Seung Hyun Jeon Eun-Joo Hahn kee-yoeup paek Jong Kwan Park Nae Young Youn Hyung-Lae Lee Department of Urology School of Medicine Kyung Hee University Seoul 134-727 Korea Research Center for the Development of Advanced Horticultural Technology Chungbuk National University Cheongju 361-711 Korea Department of Urology Chonbuk National University Medical School Jeonju 561-712 Korea Infertility Clinic Sanbornjeil Women＇s Hospital Kunpo 435-042 Korea

Korean ginseng and mountain ginseng （Panax ginseng CA Meyer） are important traditional herbal plants whose ginsenosides are generally accepted as serving to improve sexual functions, such as penile erection. We investigated the effects of tissue-cultured mountain ginseng extract （TMGE） on male patients with erectile dysfunction （ED）. A double-blind, placebo-controlled study was conducted with 143 patients experiencing ED. Over the course of 8 weeks, one group took 1 000 mg of TMGE twice a day, and the other group took 1 000 mg of placebo twice a day. The effects of the TMGE and the placebo were analyzed using the Korean version of the International Index of Erectile Function （IIEF） questionnaire. A total of 86 patients completed 8 weeks of treatment. The scores on the five domains of the IIEF after medication were significantly higher than the baseline scores in the group treated with TMGE （P 〈 0.05）, whereas no significant improvement was observed in the placebo group （P 〉 0.05）. Erectile function and overall satisfaction scores after medication were significantly higher in the TMGE group than in the placebo group （P 〈 0.05）. Erectile function of patients in the TMGE-treated group significantly improved, suggesting that TMGE could be utilized for improving erectile function in male patients.

关键词： erectile dysfunction Panax ginseng tissue-cultured mountain ginseng extract

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柴胡愈伤组织生长和不定根诱导的研究

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中草药 2007年第2期38卷 275-278页

作者：姚智高文远李克峰 paek kee-yoeup 天津大学药物科学与技术学院天津300072 Research Center for the Development of Advanced Horticultural Technology Chungbuk National University

目的探讨柴胡愈伤组织生长和其不定根诱导的适宜培养条件。方法以无菌苗为外植体诱导获得愈伤组织后,对不同种类和不同质量浓度的生长素进行实验,并对光和温度对柴胡愈伤组织生长和其不定根诱导的影响进行了研究。结果柴胡的愈伤组织适... 详细信息

目的探讨柴胡愈伤组织生长和其不定根诱导的适宜培养条件。方法以无菌苗为外植体诱导获得愈伤组织后,对不同种类和不同质量浓度的生长素进行实验,并对光和温度对柴胡愈伤组织生长和其不定根诱导的影响进行了研究。结果柴胡的愈伤组织适宜于在MS+NAA4mg/L+KT0.2mg/L培养基或MS+NAA2mg/L+KT0.2mg/L培养基上继代培养。诱导其不定根时宜采用的培养基为MS+IBA2mg/L+KT0.2mg/L。25℃条件下,黑暗培养既有利于柴胡愈伤组织的生长又有利于其不定根的诱导。结论比较适宜于柴胡愈伤组织生长的生长素为NAA,而有利于不定根诱导的生长素为IBA。温度对愈伤组织的生长影响非常明显,光照是其发根的限制因素。

关键词：柴胡愈伤组织不定根

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Establishment of Withania somnifera Hairy Root Cultures for the Production of Withanolide A

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Journal of Integrative Plant Biology 2008年第8期50卷 975-981页

作者： Hosakatte N. Murthy Camelia Dijkstra Paul Anthony Daniel A. White Mike R. Davey J. Brian Power Eun J. Hahn kee Y. paek Plant Sciences Division School of Biosciences University of Nottingham Sutton Bonington Campus Loughborough LE12 5RD UK Department of Botany Karnatak University Dharwad 580 003 India Research Center for the Development of Advanced Horticultural Technology Chungbuk National University Cheongju 361-763 Korea Department of Botany Karnatak University Dharwad 580 003 India Food Sciences Division School of Biosciences University of Nottingham Sutton Bonington Campus Loughborough LE12 5RD UK Research Center for the Development of Advanced Horticultural Technology Chungbuk National University Cheongju 361-763 Korea

Withania sominifera （Indian ginseng） was transformed by Agrobacterium rhizogenes. Explants from seedling roots, stems, hypocotyls, cotyledonary nodal segments, cotyledons and young leaves were inoculated with A. rhizogenes strain R1601. Hairy （transformed） roots were induced from cotyledons and leaf explants. The transgenic status of hairy roots was confirmed by polymerase chain reaction using nptll and rolB specific primers and, subsequently, by Southern analysis for the presence of nptll and rolB genes in the genomes of transformed roots. Four clones of hairy roots were established; these differed in their morphology. The doubling time of faster growing cultures was 8-14 d with a fivefold increase in biomass after 28 d compared with cultured, non-transformed seedling roots. MS-based liquid medium was superior for the growth of transformed roots compared with other culture media evaluated （SH, LS and N6）, with MS-based medium supplemented with 40 g/L sucrose being optimal for biomass production. Cultured hairy roots synthesized withanolide A, a steroidal lactone of medicinal and therapeutic value. The concentration of withanolide A in transformed roots （157.4 μg/g dry weight） was 2.7-fold more than in non-transformed cultured roots （57.9 μg/g dry weight）.

关键词： Agrobacterium rhizogenes hairy (transformed) roots Indian ginseng transformation withanolide A

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Commercialization of Medicinal Plant Tissue Culture

Commercialization of Medicinal Plant Tissue Culture

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第三届生物多样性保护与利用高新科学技术国际研讨会

作者： Yu kee Won paek keeyoeup Research Center for the Development of Advanced Horticultural Technology Chungbuk National University Cheongju 361-763 Korea Research Center for the Development of Advanced Horticultural Technology Chungbuk National University Cheongju 361-763 Korea

Medicinal plants have been doing great contribution to human health and they are the main part in Traditional Chinese Medicine. The traditional culture technology of medicinal plants mainly depends on

关键词： Medicinal plant Tissue culture Bioreactor Ginseng Commercialization

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Unusual Population Dynamics of the Baikal Teal Anas Formosa in Korea

Unusual Population Dynamics of the Baikal Teal Anas Formosa ...

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23rd International Ornithological Congress

作者： Hansoo Lee Woon-kee paek Si-Wan Lee Jeong-Hoon Kang Sung-Woo Han Ecotech Inst. of Environ. Ecol Daejeon 305-301 Korea National Science Museum Daejeon 305-705 Korea Ecotech Inst. of Environ. Ecol Daejeon 305-301 Korea Ecotech Inst. of Environ. Ecol Daejeon 305-301 Korea Ecotech Inst. of Environ. Ecol Daejeon 305-301 Korea

＜正＞ Population dynamics of the Baikal Teal Anas Formosa in Korea were studied during the last two decades. This population has increased over the past two decades from a few in the early 1980’s to 350,000 individuals in the winter of 2000-2001 in the nationwide winter watcrbird census. During the 1940’s, Baikal Teals were widely distributed in Korea where it was very abundant winter visitor. After that the birds were significantly decreased,

关键词： Baikal Teal Anas formosa Dynamics DDT

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A New Scale for the Decision of Surgical Management in Recurrent Glioblastomas

A New Scale for the Decision of Surgical Management in Recur...

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中国抗癌协会神经肿瘤专业委员会第八届学术会议

作者： Chul-kee Park Sang Woo Song Jin Wook Kim Yong-Hwy Kim Tae Min Kim Se-Hoon Lee Dong-Wan Kim Sun Ha paek Dae Seog Heo Dong Gyu Kim Hee-Won Jung Department of Neurosurgery Seoul National University Hospital Department of Internal Medicine Seoul National University Hospital

＜正＞Objective: To formulate the benefit of surgical management in recurrent glioblastoma, we analyzed a series of recurrent glioblastoma patients who had undergone surgery and devised a scale to predict their surviv...

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