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An efficient and universal protoplast-based transient gene expression system for genome editing in Brassica crops

Horticultural Plant Journal 2024年第4期10卷 983-994页

作者： Xiaoxiao Yu Jintai Yu Yin Lu Wenjing li Guanzhong Huo jun Zhang Yan li Jianjun Zhao jun li State key Laboratory of North China Crop Improvement and Regulation College of Life SciencesHebei Agricultural UniversityBaodingHebei 071001China Hebei key Laboratory of Plant Physiology and Molecular Pathology Hebei Agricultural UniversityBaodingHebei 071001China key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei Collaborative Innovation Center of Vegetable Industry in HebeiCollege of HorticultureHebei Agricultural UniversityBaodingHebei 071001China College of Modern Science and Technology Hebei Agricultural UniversityBaodingHebei 071001China Human Resource Department Hebei Agricultural UniversityBaodingHebei 071001China

Protoplast-based transient gene expression system has been widely used in plant genome editing because of its simple operation and less *** order to establish a universal protoplast-based transient transfection system for verifying activities of genome editing vectors containing targets in Brassica,we systematically optimized factors affecting protoplast isolation and transient gene *** established an efficient protoplast-based transient gene expression system(PTGE)in Chinese cabbage,achieving high protoplast yield of 4.9×10^(5)·g^(-1)FW,viability over 95%,and transfection efficiency of 76%.We showed for the first time that pretreatment of protoplasts with a hypotonic MMG could significantly enhance the transfection ***,protoplasts incubated at 37℃ for 6 min improved the transfection efficiency to 86%.We also demonstrated that PTGE worked well(more than 50%transfection efficiency)in multiple Brassica species including cabbage,Pak Choi,Chinese kale,and ***,PTGE was used for validating the activities of CRISPR/Cas9 vectors containing targets in Chinese cabbage,cabbage,and pak choi,demonstrating the broad applicability of the established PTGE for genome editing in Brassica crops.

关键词： Chinese cabbage PTGE Transfection efficiency CRISPR/Cas9

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Hole cleaning evaluation and installation spacing optimization of cuttings bed remover in extended-reach drilling

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Petroleum Science 2024年第3期21卷 2005-2022页

作者： Shuo Peng Wen-jun Huang De-li Gao MOE key Laboratory of Petroleum Engineering China University of Petroleum-Beijing State key Laboratory of Petroleum Resources and Engineering China University of Petroleum-Beijing

In extended-reach or long-horizontal drilling, cuttings usually deposit at the bottom of the annulus. Once cuttings accumulate to a certain thickness, complex problems such as excessive torque and drag, tubing buckling, and pipe stuck probably occur, which results in a lot of non-productive time and remedial operations. Cuttings bed remover can efficiently destroy deposited cuttings in time through hydraulic and mechanical stirring effects. This paper aims to build a method for hole cleaning evaluation and installation spacing optimization of cuttings bed remover to improve the wellbore cleaning effect. Firstly,a Computational Fluid Dynamics approach with Eulerian—Eulerian multiphase model was utilized to investigate the mechanism of cuttings transportation, and a new type of cuttings bed remover was designed. Next, an evaluation method of hole cleaning effect of remover was established. After that, the effects of several drilling parameters on hole cleaning including flow rate of drilling fluid, rotational speed of drillpipe, rate of penetration, wellbore size, rheological property of drilling fluid, and remover eccentricity on the performance of cuttings bed remover were investigated. The results demonstrate that the new type of remover with streamline blade performs better than conventional removers. The efficiency of hole cleaning is greatly improved by increasing the rotational speed of drillpipe, flow rate of drilling fluid, remover eccentricity, and 6 rpm Fann dial reading for drilling fluid. While higher rate of penetration and large wellbore size result in worse hole cleaning. These findings can serve as an important guide for the structure optimization design of cuttings bed remover and installation spacing of removers.

关键词： Extended-reach drilling Drilling hydraulics Cuttings transport Hole cleaning Cuttings bed remover

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Unique double-layer solid electrolyte interphase formed with fluorinated ether-based electrolytes for high-voltage lithium metal batteries

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Journal of Energy Chemistry 2024年第1期88卷 532-542,I0012页

作者： Ruo Wang Jiawei li Bing Han Qingrong Wang Ruohong ke Tong Zhang Xiaohu Ao Guangzhao Zhang Zhongbo liu Yunxian Qian Fangfang Pan Iseult Lynch jun Wang Yonghong Deng Department of Materials Science&Engineering School of Innovation and EntrepreneurshipSouthern University of Science and TechnologyShenzhen 518055GuangdongChina School of Geography Earth and Environmental SciencesUniversity of BirminghamEdgbastonBirmingham B152TTUK key Laboratory of Marine Environmental Corrosion and Bio-Fouling Institute of OceanologyChinese Academy of SciencesQingdao 266071ShandongChina Shenzhen CAPCHEM Technology Co. Ltd.Shenzhen 518118GuangdongChina CALB Group Co. Ltd.Changzhou 213200JiangsuChina

li metal batteries using high-voltage layered oxides cathodes are of particular interest due to their high energy ***,they suffer from short lifespan and extreme safety concerns,which are attributed to the degradation of layered oxides and the decomposition of electrolyte at high voltage,as well as the high reactivity of metallic *** key is the development of stable electrolytes against both highvoltage cathodes and li with the formation of robust interphase films on the ***,we report a highly fluorinated ether,1,1,1-trifluoro-2-[(2,2,2-trifluoroethoxy)methoxy]ethane(TTME),as a cosolvent,which not only functions as a diluent forming a localized high concentration electrolyte(LHCE),but also participates in the construction of the inner solvation *** TTME-based electrolyte is stable itself at high voltage and induces the formation of a unique double-layer solid electrolyte interphase(SEI)film,which is embodied as one layer rich in crystalline structural components for enhanced mechanical strength and another amorphous layer with a higher concentration of organic components for enhanced *** li||Cu cells display a noticeably high Coulombic efficiency of 99.28%after 300 cycles and li symmetric cells maintain stable cycling more than 3200 h at 0.5 mA/cm^(2) and 1.0m Ah/cm^(2).In addition,lithium metal cells using liNi_(0.8)Co_(0.1)Mn_(0.1)O_(2) and li CoO_(2) cathodes(both loadings~3.0 m Ah/cm^(2))realize capacity retentions of>85%over 240 cycles with a charge cut-off voltage of 4.4 V and 90%for 170 cycles with a charge cut-off voltage of 4.5 V,*** study offers a bifunctional ether-based electrolyte solvent beneficial for high-voltage li metal batteries.

关键词： lithium metal batteries High-voltage layered oxides Fluorinated ether-based electrolytes Solid electrolyte interphase Cathode electrolyte interphase

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Kinetic and mechanism of the reaction between Cl and several mono-methyl branched alkanes

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Journal of Environmental Sciences 2024年第1期135卷 474-482页

作者： Yan Chen Weigang Wang junling li li Zhou Bo Shi Cici Fan ke Wang Hao Zhang Hong li Maofa Ge State key Laboratory for Structural Chemistry of Unstable and Stable Species Beijing National Laboratory for Molecular Sciences(BNLMS)CAS Research/Education Center for Excellence in Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China State key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental SciencesBeijing 100012China National Engineering Research Center for Flue Gas Desulfurization Department of Environmental Science and EngineeringSichuan UniversityChengdu 610065China College of Chemistry and Materials Science Hebei Normal UniversityShijiazhuang 050024China

Branched alkanes are ubiquitous in the troposphere and play an important role in the chemical *** this work,the rate constants and products for the reaction of Cl atoms with 3-methylhexane and 2-methylheptane were measured at room temperature(298±0.2K)and atmospheric pressure using a conventional relative rate *** rate constants of 3-methylhexane and 2-methylheptane in units of cm~3/(mol·sec)are(3.09±0.31)×10^(-10)and(3.67±0.40)×10^(-10),***,the corresponding atmospheric lifetime of the studied branched alkanes with Cl was 6.92-89.90 hours and 5.82-75.69 hours,*** estimated atmospheric lifetimes indicated that the reaction with Cl atoms could be the most important atmospheric degradation pathway for 3-methylhexane *** gas-phase products of the reactions were identified and quantified,and particle-phase products were also *** atmosphere oxidation mechanism of Cl atoms with 3-methylhexane and 2-methylheptane is *** SOA yields of 3-methylhexane and 2-methylheptane from the reaction of Cl atoms were determined to be7.96%±0.89%and 13.35%±1.50%***,the results reveal that the primary loss process of branched alkanes is the reaction with Cl atoms,which impacts its degradation on a regional scale.

关键词： 3-Methylhexane 2-Methylheptane Chlorine atoms Kinetics Mechanism

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On the Quantum Cohomology of Blow-ups of Four-dimensional Quadrics

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Acta Mathematica Sinica,English Series 2024年第1期40卷 313-328页

作者： Jian Xun HU Hua Zhong ke Chang Zheng li Lei SONG School of Mathematics Sun Yat-sen UniversityGuangzhou 510275P.R.China

We propose a conjecture relevant to Galkin’s lower bound conjecture,and verify it for the blow-ups of a four-dimensional quadric at a point or along a projective *** also show that Conjecture O holds in these two cases.

关键词： Quantum cohomology blow-up quadric hypersurface

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Effect of Phase Separation Size on the Properties of Self-healing Elastomer

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Chinese Journal of Polymer Science 2024年第6期42卷 798-804页

作者： jun Xu Lei Zhu Xian-Qi Feng Cong Sui Wen-Peng Zhao Shou-ke Yan School of Chemical Engineering Qingdao University of Science&TechnologyQingdao266042China College of Engineering Yanching institute of TechnologyLangfang065210China key Laboratory of Rubber-plastics Qingdao University of Science&TechnologyQingdao266042China State key Laboratory of Chemical Resource Engineering Beijing University of Chemical TechnologyBeijing100029China

Regulation of phase structure has been recognized as one of the most effective ways to fabricate self-healing polymers with high mechanical *** mechanical properties of the resultant polymers are certainly affected by the size of separated phase ***,the study on this aspect is absence,because it can hardly exclude the influence of variation in monomer proportion required for tuning the separated phase ***,we report the first study on tuning the phase size through reversible addition-fragmentation chain transfer(RAFT)polymerization without changing the proportion of *** expected,the size of separated phase has been successfully mediated from 15 nm to 9 nm by tuning the molecular weight of the chain transfer *** is found that the mechanical strength and the self-healing efficiency of the resultant polymers increase simultaneously with the decrease of phase *** study on the formation kinetics of hydrogen bonds reveals that the decrease of phase size can facilitate the re-bonding rate of hydrogen bonds,even if the migration of polymer chains is restricted.

关键词： Self-healing polymers Phase separation Reversible addition-fragmentation chain transfer

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Fibroblast growth factor 21: An emerging pleiotropic regulator of lipid metabolism and the metabolic network

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Genes & Diseases 2024年第3期11卷 248-263页

作者： Shuo li Tiande Zou jun Chen Jiaming li Jinming You Jiangxi Province key Laboratory of Animal Nutrition Jiangxi Agricultural UniversityNanchangJiangxi 330045China

Fibroblast growth factor 21(FGF21)was originally identified as an important meta-bolic regulator which plays a crucial physiological role in regulating a variety of metabolic pa-rameters through the metabolic *** a novel multifunctional endocrine growth factor,the role of FGF21 in the metabolic network warrants extensive *** insight was obtained from the observation that the FGF21-dependent mechanism that regulates lipid metabolism,glycogen transformation,and biological effectiveness occurs through the coordi-nated participation of the liver,adipose tissue,central nervous system,and sympathetic *** review focuses on the role of FGF21-uncoupling protein 1(UCP1)signaling in lipid metabolism and how FGF21 alleviates non-alcoholic fatty liver disease(NAFLD).Additionally,this review reveals the mechanism by which FGF21 governs glucolipid *** research on the role of FGF21 in the metabolic network has mostly focused on the crucial pathway of glucolipid ***21 has been shown to have multiple regulatory roles in the metabolic *** an adequate understanding of the concrete regulatory path-ways of FGF21 in the metabolic network has not been attained,this review sheds new light on the metabolic mechanisms of FGF21,explores how FGF21 engages different tissues and organs,and lays a theoretical foundation for future in-depth research on FGF21-targeted treatment of metabolic diseases.

关键词： Adipose FGF21 Insulin homeostasis lipid metabolism liver Metabolicnetwork

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light(Science & Applications) 2024年第2期13卷 333-346页

作者： Yifan Sun Qian li ling-jun Kong Xiangdong Zhang key Laboratory of advanced optoelectronic quantum architecture and measurements of Ministry of Education Beijing Key Laboratory of Nanophotonics&Ultrafine Optoelectronic SystemsSchool of PhysicsBeijing Institute of Technology100081BeijingChina

Compared with electrical neural networks,optical neural networks(ONNs)have the potentials to break the limit of the bandwidth and reduce the consumption of energy,and therefore draw much attention in recent *** far,several types of ONNs have been ***,the current ONNs cannot realize the acceleration as powerful as that indicated by the models like quantum neural *** to construct and realize an ONN with the quantum speedup is a huge ***,we propose theoretically and demonstrate experimentally a new type of optical convolutional neural network by introducing the optical *** is called the correlated optical convolutional neural network(COCNN).We show that the COCNN can exhibit“quantum speedup”in the training *** character is verified from the two *** is the direct illustration of the faster convergence by comparing the loss function curves of the COCNN with that of the traditional convolutional neural network(CNN).Such a result is compatible with the training performance of the recently proposed quantum convolutional neural network(QCNN).The other is the demonstration of the COCNN’s capability to perform the QCNN phase recognition circuit,validating the connection between the COCNN and the ***,we take the COCNN analog to the 3-qubit QCNN phase recognition circuit as an example and perform an experiment to show the soundness and the feasibility of *** results perfectly match the theoretical *** proposal opens up a new avenue for realizing the ONNs with the quantum speedup,which will benefit the information processing in the era of big data.

关键词： process quantum convolution

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Control of temperature dependent viscosity for manufacturing of Bi-doped active fiber

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International Journal of Extreme Manufacturing 2024年第3期6卷 522-530页

作者： Rui Duan Jingfei Chen Hao ke Tianxia Wei ke Zhang Xueliang li Xu Feng Qiuju Zheng Zhixue He Jianrong Qiu Shifeng Zhou State key Laboratory of Luminescent Materials and Devices School of Materials Science and EngineeringSouth China University of TechnologyGuangzhou 510640People’s Republic of China Guangdong Provincial key Laboratory of Fiber Laser Materials and Applied Techniques Guangdong Engineering Technology Research and Development Center of Special Optical Fiber Materials and DevicesGuangzhou 510640People’s Republic of China School of Materials Science and Engineering Qilu University of TechnologyJinanPeople’s Republic of China Peng Cheng Laboratory Shenzhen 518000People’s Republic of China College of Optical Science and Engineering State Key Laboratory of Modern Optical InstrumentationZhejiang UniversityHangzhou 310027People’s Republic of China

Bi-activated photonic materials are promising for various applications in high-capacity telecommunication,tunable laser,and advanced bioimaging and *** various Bi-doped material candidates have been explored,manufacturing of Bi heavily doped fiber with excellent optical activity remains a long-standing ***,a novel viscosity evolutional behavior mediated strategy for manufacturing of Bi-doped active fiber with high dopant solubility is *** intrinsic relation among the evolution of Bi,reaction temperature and viscosity of the glass system is ***,the effective avenue to prevent the undesired deactivation of Bi during fiber drawing by tuning the temperature dependent viscosity evolution is *** applying the strategy,for the first time we demonstrate the success in fabrication of heavily doped Bi active ***,the principal fiber amplifier device is constructed and broadband optical signal amplification is *** findings indicate the effectiveness of the proposed temperature dependent viscosity mediated strategy for developing novel photonic active fiber,and they also demonstrate the great potential for application in the next-generation high-capacity telecommunication system.

关键词： infrared luminescence active fiber optical amplification

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Consistency of Tropospheric Water Vapor between Reanalyses and Himawari-8/AHI Measurements over East Asia

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Advances in Atmospheric Sciences 2024年第1期41卷 19-38页

作者： Di DI jun li Yunheng XUE Min MIN Bo li Zhenglong li Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters Nanjing University of Information Science and TechnologyNanjing 210044China key Laboratory for Aerosol-Cloud-Precipitation China Meteorological AdministrationSchool of Atmospheric PhysicsNanjing University of Information Science and TechnologyNanjing 210044China FYSIC National Satellite Meteorological CenterChina Meteorological AdministrationBeijing 100081China Chengdu University of Information Science and Technology Chengdu 610225China School of Atmospheric Sciences and Guangdong Province key laboratory for Climate Change and Natural Disaster Studies Sun Yat-sen University and Southern Laboratory of Ocean Science and EngineeringZhuhai 519082China Cooperative Institute for Meteorological Satellite Studies University of Wisconsin–MadisonMadison WI 53706WisconsinUSA Wuxi University Wuxi 214105China

High spatiotemporal resolution radiances from the advanced imagers onboard the new generation of geostationary weather satellites provide a unique opportunity to evaluate the abilities of various reanalysis datasets to depict multilayer tropospheric water vapor(WV),thereby enhancing our understanding of the deficiencies of WV in reanalysis *** on daily measurements from the Advanced Himawari Imager(AHI)onboard the Himawari-8 satellite in 2016,the bias features of multilayer WV from six reanalysis datasets over East Asia are thoroughly *** assessments show that wet biases exist in the upper troposphere in all six reanalysis datasets;in particular,these biases are much larger in ***,we find better depictions of WV in the middle troposphere than in the upper *** accuracy of WV in the ERA5 dataset is the highest,in terms of the bias magnitude,dispersion,and pattern *** characteristics of the WV bias over the Tibetan Plateau are significantly different from those over other parts of East *** addition,the reanalysis datasets all capture the shift of the subtropical high very well,with ERA5 performing better overall.

关键词： AHI reanalysis dataset multilayer water vapor assessment radiative transfer model

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