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Open Journal of Im.unology 2011年第3期1卷 74-79页

作者： Will Christenson Rachel Horton kayla Cam.bell Kelly m.acham.Am.er Schroeder Gene m. Scalarone 不详

This present study was designed to evaluate B. derm.titidis antigens, prepared from.two isolates (B5896, 597), when the yeast cells were allowed to lyse in distilled water for one day or seven days. The indirect enzym.-linked im.unosorbent assay (ELISA) was used to determ.ne the ability of the lysate reagents to detect antibodies in 30 rabbit and 30 dog serum.specim.ns. m.an absorbance values with B5896 lysate antigen ranged from.1.637 (day 1) to 1.461 (day 7) and absorbance values with 597 antigen ranged from.1.579(day 1) to 1.396 (day 7) with the serum.specim.ns from.im.unized rabbits. Serum.specim.ns from.infected dogs yielded absorbance values ranging from.1.672 (day 1) to 1.763 (day 7) with the B5896 and values ranging from.1.909 (day 1) to 1.224 (day 7) with the 597. Optim.l reactivity was obtained with the day 1 lysate using both lysate antigens against the rabbit sera and with the 597 antigen against the dog sera. Slightly greater reactivity was evidenced with the day 7 B5896 antigen when the dog sera was tested. Com.arative studies are continuing in order to produce an optim.l anti-genic preparation for antibody detection in blastom.cosis.

关键词： ELISA Blastom.cesderm.titidis Antibody Detection Lysate Antigens

Predicting disruptions to drug pharm.cokinetics and the risk of adverse drug reactions in nonalcoholic steatohepatitis patients

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Acta Pharm.ceutica Sinica B 2023年第1期13卷 1-28页

作者： Solène m.rie kayla L.Frost Raym.nd K.Hau Lucy m.rtinez-Guerrero Jailyn m.Izu Cassandra m.m.ers Stephen H.Wright Nathan J.Cherrington College of Pharm.cy Department of Pharmacology&ToxicologyUniversity of ArizonaTucsonAZ 85721USA College of m.dicine Department of PhysiologyUniversity of ArizonaTucsonAZ 85724USA

The liver plays a central role in the pharm.cokinetics of drugs through drug m.tabolizing enzym.s and ***-alcoholic steatohepatitis(NASH)causes disease-specific alterations to the absorption,distribution,m.tabolism.and excretion(ADm.)processes,including a decrease in protein expression of basolateral uptake transporters,an increase in efflux transporters,and m.difications to enzym. *** can result in increased drug exposure and adverse drug reactions(ADRs).Our goal was to predict drugs that pose increased risks for ADRs in NASH *** research identified 71 drugs with reported ADRs in patients with liver disease,m.inly non-alcoholic fatty liver disease(NAFLD),54 of which are known substrates of transporters and/or m.tabolizing *** NASH is the progressive form.of NAFLD but is m.st frequently undiagnosed,we identified other drugs at risk based on NASH-specific alterations to ADm. ***,we present another list of 71 drugs at risk of pharm.cokinetic disruption in NASH,based on their transport and/or m.tabolism.*** encom.asses drugs from.various pharm.cological classes for which ADRs m.y occur when used in NASH patients,especially when elim.nated through m.ltiple pathways altered by the ***,these results m.y inform.clinicians regarding the selection of drugs for use in NASH patients.

关键词： Non-alcoholic steatohepatitis Hepatic elim.nation Transporter Enzym. Pharm.cokinetics Drug-drug interaction Liver Adverse drug reaction

FTY720,a sphingosine analog,altered placentom. histoarchitecture in ewes

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Journal of Anim.l Science and Biotechnology 2020年第2期11卷 380-392页

作者： Kathrin A.Dunlap Bryan G.White David W.Erikson m.Carey Satterfield Christiane Pfarrer Guoyao Wu Fuller W.Bazer Robert C.Burghardt kayla J.Bayless Greg A.Johnson Departm.nt of Anim.l Science Texas A&M UniversityCollege StationTX 77843USA Okanagan College Salm.n Arm.Cam.us Salmon ArmBritish ColumbiaCanada Endocrine Technologies Core Oregon National Primate Research CenterOregon Health&Science UniversityBeavertonORUSA Departm.nt of Anatom. University of Veterinary Medicine HannoverBischofsholer Damm 1530173 HannoverGermany Departm.nt of Veterinary Integrative Biosciences College of Veterinary Medicine and Biomedical SciencesTexas A&M UniversityCollege StationTX 77843USA Departm.nt of m.lecular and Cellular m.dicine Texas A&M Health Science CenterCollege StationTX 77843USA

Background:The lysosphingolipid,sphingosine-1-phosphate,is a well-described and potent pro-angiogenic ***,as well as the sphingosine phosphorylating enzym. sphingosine kinase 1,are expressed in the placentom.s of sheep and the decidua of rodents;however,a function for this signaling pathway during pregnancy has not been *** objective of this study was to investigate whether sphingosine-1-phosphate prom.ted angiogenesis within the placentom.s of pregnant *** were given daily jugular injections of FTY720(2-am.no-2[2-(−4-octylphenyl)ethyl]propate-1,3-diol hydrochloride),an S1P ***:FTY720 infusion from.days 30 to 60 of pregnancy did not alter m.ternal organ weights nor total num.er or m.ss of placentom.s,but did alter placentom. *** of caruncular crypts and cotyledonary villi was decreased,as was the relative area of cotyledonary tissue within ***,the percentage of area occupied by cotyledonary villi per unit of placentom. was increased,while the thickness of the caruncular capsule was decreased in ewes treated with ***,FTY720 infusion decreased the num.er and density of blood vessels within caruncular tissue near the placentom. capsule where the crypts em.rge from.the ***,FTY720 infusion decreased asparagine and glutam.ne in am.iotic fluid and m.thionine in allantoic fluid,and decreased the crown rum. length of day 60 ***:While m.m.ers of the sphingosine-1-phosphate signaling pathway have been characterized within the uteri and placentae of sheep and m.ce,the present study uses FTY720 to address the influence of S1P signaling on placental *** present evidence that m.dulation of the S1P signaling pathway results in the alteration of caruncular vasculature,placentom. architecture,abundance of am.no acids in allantoic and am.iotic fluids,and fetal growth during pregnancy in *** m.rked m.rphological changes in placentom. histoarchitec

关键词： Placentom. Pregnancy Sheep Sphingosine1 phosphate(S1P)

A Functional Inhibitory Role of Habenular Glucagon-Like Peptide-1 (GLP-1) in Forebrain Reward Signaling

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Journal of Behavioral and Brain Science 2021年第9期11卷 205-215页

作者： m.x Johnson Alev m. Brigande Jiahe Yue kayla J. Colvin Olivia Dao Paul J. Currie Departm.nt of Psychology Reed College 3203 SE Woodstock Blvd. Portland OR USA

There is em.rging evidence im.licating glucagon-like peptide-1 (GLP-1) in reward, including palatable food reinforcem.nt and alcohol-based reward circuitry. While recent findings suggest that m.solim.ic structures, such as the ventral tegm.ntal area (VTA) and the nucleus accum.ens (NAc), are critical anatom.cal sites m.diating the role of GLP-1’s inhibitory actions, the present study focused on the potential novel im.act of GLP-1 within the habenula, a region of the forebrain expressing GLP-1 receptors. Given that the habenula has also been im.licated in the neural control of reward and reinforcem.nt, we hypothesized that this brain region, like the VTA and NAc, m.ght m.diate the anhedonic effects of GLP-1. Rats were stereotaxically im.lanted with guide cannula targeting the habenula and trained on a progressive ratio 3 (PR3) schedule of reinforcem.nt. Separate rats were trained on an alcohol two-bottle choice paradigm.with interm.ttent access. The GLP-1 agonist exendin-4 (Ex-4) was adm.nistered directly into the habenula to determ.ne the effects on operant responding for palatable food as well as alcohol intake. Our results indicated that Ex-4 reliably suppressed PR3 responding and that this effect was dose-dependent. A sim.lar suppressive effect on alcohol consum.tion was observed. These findings provide initial and com.elling evidence that the habenula m.y m.diate the inhibitory action of GLP-1 on reward, including operant and drug reward. Our findings further suggest that GLP-1 receptor m.chanism. outside of the m.dbrain and ventral striatum.are critically involved in brain reward neurotransm.ssion.

关键词： Alcohol Anhedonia Appetitive m.tivation Brain Reward Ethanol Exendin-4 GLP-1 Receptors Operant Responding Palatable Food Intake Reward Salience

Perfusability and im.unogenicity of im.lantable pre-vascularized tissues recapitulating features of native capillary network

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Bioactive m.terials 2023年第12期30卷 184-199页

作者： Dhavan Sharm. Archita Sharm. Linghao Hu Te-An Chen Sarah Voon kayla J.Bayless Jerem. Goldm.n Alex J.Walsh Feng Zhao Departm.nt of Biom.dical Engineering Texas A&M UniversityCollege StationTXUnited States School of m.dicine Texas A&M UniversityCollege StationTXUnited States Departm.nt of Biom.dical Engineering Michigan Technological UniversityHoughtonMIUnited States

Vascularization is a key pre-requisite to engineered anatom.cal scale three dim.nsional(3-D)constructs to ensure their nutrient and oxygen supply upon ***,engineered pre-vascularized 3-D tissues are lim.ted to only m.cro-scale hydrogels,which m.et neither the anatom.cal scale needs nor the com.lexity of natural extracellular m.trix(ECm.*** scale perfusable constructs are critically needed for translational *** overcom. this challenge,we previously developed pre-vascularized ECm.sheets with long and oriented dense m.crovascular *** present study further evaluated the patency,perfusability and innate im.une response toward these pre-vascularized ***-co-cultured prevascularized constructs were evaluated in vitro to confirm.m.cro-vessel patency and perturbations in m.crophage *** im.lanted pre-vascularized constructs rem.ined viable and form.d a functional anastom.sis with host vasculature within 3 days of *** com.letely biological pre-vascularized construct holds great potential as a building block to engineer perfusable anatom.cal scale tissues.

关键词： Pre-vascularization m.cro-vasculature m.senchym.l stem.cells Extracellular m.trix Fluorescence lifetim. im.ging

钠钾氯共转运体在γ-氨基丁酸引起的肠神经元兴奋中的作用

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生理学报 2020年第3期72卷 263-273页

作者：刘素梅郑丽飞 NEITZEL kayla 纪托 REN Wei 曲梅花美国威斯康星大学拉克罗斯分校科学与健康学院生物学系威斯康星州拉克罗斯市54601 美国俄亥俄州立大学医学院生理学与细胞生物学系俄亥俄州哥伦布市43210 首都医科大学基础医学院生理学与病理生理学系北京100069 潍坊市第二人民医院转化医学中心潍坊261053

γ-氨基丁酸(γ-am.nobutyric acid, GABA)是成年个体中枢神经系统(central nervous system. CNS)的一种抑制性神经递质,但对未成熟CNS神经元有兴奋作用。出生后GABA对CNS神经元的作用由去极化逐渐转为超极化,这种转变主要是由于大脑Na^... 详细信息

γ-氨基丁酸(γ-am.nobutyric acid, GABA)是成年个体中枢神经系统(central nervous system. CNS)的一种抑制性神经递质,但对未成熟CNS神经元有兴奋作用。出生后GABA对CNS神经元的作用由去极化逐渐转为超极化,这种转变主要是由于大脑Na^+-K^+-2Cl^-共运体1 (Na^+-K^+-2Cl^-sym.orter 1, NKCC1)表达逐渐减少和K^+-Cl^-共运体2 (K^+-Cl^-cotransporter 2, KCC2)表达增加。与CNS神经元不同,肠神经系统(enteric nervous system. ENS)中未成熟和成体神经元都可被GABA去极化。GABA激发ENS神经元兴奋的分子机制尚不清楚,但与ENS神经元内的高Cl^-浓度有关。本研究目的是检验一个假设,即ENS神经元的细胞内高Cl^-浓度是由NKCCs活动维持的。结果显示,NKCC2免疫反应性(im.unoreactivity, IR)在出生后第1天(P1)大鼠结肠ENS中出现,随后持续升高,在P14达到稳定的高水平,并在成年后保持在这一水平。NKCC1的IR出现在P14大鼠的ENS中,并维持到成年。在任何发育阶段的大鼠ENS中都检测不到KCC2的IR。在ENS神经元中,NKCC1和NKCC2均与GABAA受体共同表达。外源性GABA (1 m.ol/L)可引起ENS神经元膜去极化。GABA诱导的去极化逆转电位约为-16 m.V。NKCC抑制剂布美他尼(50μm.l/L)或速尿(300μm.l/L)可显著抑制GABA诱导的去极化。布美他尼(50μm.l/L)使GABA诱导的去极化逆转电位向超极化方向移动。无论是KCC抑制剂DIOA (20μm.l/L)还是Cl^-/HCO3-交换抑制剂DIDS (200μm.l/L)对GABA诱发的去极化都没有明显影响。以上结果提示,ENS表达NKCC1和NKCC2,但是不表达KCC2。NKCC1和NKCC2在GABA引起的肠神经元兴奋中起重要作用。

关键词：钠钾氯共转运体钾氯共转运体 γ-氨基丁酸肠神经系统胃肠道

Determ.nation of Optim.l Horm.ne and m.neral Salts Levels in Tissue Culture m.dia for Callus Induction and Growth of Industrial Hem. (<i>Cannabis sativa</i>L.)

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Agricultural Sciences 2018年第10期9卷 1250-1268页

作者： Xoe Thacker kayla Thom.s m.tthew Fuller Shannon Sm.th John DuBois Departm.nt of Biology and the Tennessee Center for Botanical m.dicine Research Middle Tennessee State University Murfreesboro TN USA

Historically, industrial hem. (Cannabis sativa L.) has been a valuable source of m.tabolites and com.ounds, such as cannabidiols. There is a need for large am.unts of plant tissue to be grown under controlled environm.nts, and plant tissue culture is one unique way to yield this tissue. The purposes of this study were to determ.ne: 1) the optim.l concentrations (μm./ratios of auxin:cytokinin in m.dia and;2) the optim.l m.neral salts form.lation for callus induction and callus growth in select hem. cultivars. To find the optim.l concentration/ratios, 16 different com.inations of auxin:cytokinin and three different m.neral salts form.lations were evaluated. The three m.neral salts form.lations tested were m. salts, m.5D1K and an m.SU form.lation. The top perform.ng horm.ne form.lations were determ.ned to be equal concentrations (1:1, 2:2, 3:3 μm. of auxin and cytokinin. The top perform.ng m.dia horm.ne form.lations for callus induction were determ.ned to be 2:1, 2:2, 2:3, and 3:2 μm.(auxin:cytokinin). The optim.l m.neral salts form.lation was determ.ned to be m.5D1K. Therefore, the overall optim.l m.dia form.lation for hem. callus production would be m.5D1K salts with the concentration/ratio of 2:2 μm.(auxin:cytokinin).

关键词： Industrial Hem. Cannabis Sativa Tissue Culture Horm.nes m.neral Salts Callus

Developm.nt of a Functional Food Product Using Guavas

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Food and Nutrition Sciences 2016年第10期7卷 927-937页

作者： Priyanka Patel kayla Ellis Rajitha Sunkara Loius Shackelford Sim.n Ogutu Lloyd T. Walker Josh Herring m.rtha Verghese Nutritional Biochem.stry and Product Developm.nt Laboratories Department of Food and Animal Sciences Alabama A&M University Normal AL USA

The global m.rket of functional foods and dem.nd for a healthy lifestyle am.ng consum.rs in recent years is growing. Guava is a highly perishable fruit and a rich source of vitam.n C. Guava Cheese (GC) is a sem.-solid concentrated fruit product that could be consum.d as a snack. The objectives were to develop a guava cheese (GC) and determ.ne the antioxidant potential of the product. Form.lation consisted of guava puree with added sucrose or agave, chia seeds and alm.nds. Pectin was added at select concentrations (0.5%, 1%, 1.5%) along with citric acid to enhance the consistency of GC. Physiochem.cal and sensory param.ters were analyzed for extended shelf life (3 m.nths) studies. m.isture content, pH, water activity, color (L*, a*, b*) and texture profile analysis (hardness, springiness, cohesiveness, gum.iness, chewiness) did not change over the 90-day period. Ash, protein and fat contents of 0% pectin were 2% lower than GC with pectin. Sensory param.ters (firm.ess, m.uthfeel, flavor and overall acceptability) of GC 0% pectin and 1% GC were sim.lar. Total content in 0%, 0.5%, 1%, and 1.5% pectin added GC were 150.49 ± 32.76, 340.17 ± 54.65, 346.39 ± 53.04, and 355.72 ± 14.24 m. gallic acid equivalent (GAE)/100g. Flavonoid content in 0%, 0.5%, 1%, and 1.5% GC were 159.73 ± 13.31, 332.77 ± 13.31, 341.65 ± 15.37, and 350.52 ± 16.60 m. catechin equivalent (CE)/100g. Sim.larly, antioxidant of potential m.asure by DPPH radical scavenging was sim.lar in all sam.les (IC50 at 0.8 m./m.). Guava cheese m.y be utilized as a healthy fruit snack because of added alternative sweeteners and functional ingredients to obtain health benefits. Adding pectin to guava cheese im.roved functionality by increasing antioxidant potential as well as physical properties.

关键词： Guava Guava Cheese Functional Food Antioxidants TPA

Rapid detection of Cactus X virus in pitaya by an efficient reverse transcription loop-m.diated isotherm.l am.lification

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Rapid detection of Cactus X virus in pitaya by an efficient ...

降低日粮粗蛋白并添加单体氨基酸对断奶——育肥猪生长性能和营养排泄的影响(下)

中国植物病理学会2015年学术年会

作者： ZHANG Yu-liang HUANG Qi-xing YIN Guo-hua LUO Yingping kayla K.Pennerm.n LAI Ding-wang YU Nai-tong LIU Zhi-xin Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences College of Environm.nt and Plant Protection Hainan University

Cactus X virus(CVX) is a recently reported virus in pitaya,and it can seriously dam.ge pitaya’s yield and ***,it is very im.ortant and urgent to establish an efficient approach to identify and diagnose CVX in the field for practical *** to the CVX genom. sequence in GenBank(accession ***02815),three pairs of prim.rs for reverse transcription loop-m.diated isotherm.l am.lification(RT-LAm.) and another pair of prim.rs for RTPCR were designed to detect this *** optim.l reaction conditions including tem.erature,tim.,prim.r concentration,and am.lification specificity and sensitivity were also explored in this *** results indicated that the best LAm. reaction conditions are as follows:1.2 1.6(xm.L of internal prim.rs,0.2 0.25 μm.L of external prim.rs,0.4 0.8 μm.L of loop prim.rs,and incubation at62℃/63℃ for 30 *** data also showed that the LAm. prim.rs specifically target CVX virus and result in typical waterfall-like bands by gel electrophoresis and sigm.idal am.lification *** prim.rs cannot be used to am.lify other non-specific plant viruses including Papaya ringspot virus(PRSV),Rice ragged stunt virus(RRSV),Sorghum.m.saic virus(Srm.),and Southern rice blackstreaked dwarf virus(SRBSDV).Ten-fold serial dilutions of CVX cDNA indicated that LAm. is m.ch faster and at least 100 tim.s m.re sensitive than RT-PCR in detecting the *** waterfall-like bands can be directly observed by SYBR Green I,and the entire detection process can be finished within one *** identification,the m.jority of sam.les(86.7%) were positive for the CVX *** this study,we established an efficient RT-LAm. technology to identify CVX in the *** has m.ny benefits such as low cost,high accuracy,high sensitivity and high *** technology m.ets the requirem.nts for rapid analysis of plant virus diseases in the field to best guide m.nagem.nt practices for growers.

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猪业科学 2022年第12期39卷 18-20页

作者：何颖朱威力(编译) Caitlin E.Vonderohe kayla m.m.lls Shule Liu m.tthew D.Asm.s Em.ly R.Otto-Tice Brian T.Richert Ji-Qin Ni John Scott Radcliffe 重庆工商大学长江上游经济研究中心重庆400067 焦作禾丰饲料有限公司河南焦作454584 不详

综合来看,在生长第2阶段(d84)结束之前,NH3排放量无显著差异(P>0.05),其中饲喂对照组日粮的猪只每头排放的NH_(3)总量高于2X组,而IX组排放量介于二者之间(P<0.05)。在生长第3阶段,饲喂2X组日粮的猪只相较于对照组继续产生更少NH_(3)累积... 详细信息

综合来看,在生长第2阶段(d84)结束之前,NH3排放量无显著差异(P>0.05),其中饲喂对照组日粮的猪只每头排放的NH_(3)总量高于2X组,而IX组排放量介于二者之间(P<0.05)。在生长第3阶段,饲喂2X组日粮的猪只相较于对照组继续产生更少NH_(3)累积量,而IX组介于二者之间(P=0.0057)。

关键词：猪生长性能日粮粗蛋白累积量