检索结果-南通市图书馆

Ca^(2+) binding to the C_(2)E domain of otoferlin is required for hair cell exocytosis and hearing

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protein & Cell 2024年第4期15卷 305-312页

作者： Han Chen Mehar Monga Qinghua Fang Loujin Slitin Jakob Neef Shashank S.Chepurwar Regina Célia Mingroni Netto Karina Lezirovitz Alfredo Tabith,Jr. Fritz Benseler Nils Brose kathrin Kusch Carolin Wichmann Nicola Strenzke Barbara Vona Julia Preobraschenski Tobias Moser Institute for Auditory Neuroscience and InnerEarLab University Medical Center Göttingen37075 GöttingenGermany Collaborative Research Center 889 University of Göttingen37075 GöttingenGermany Auditory Neuroscience and Synaptic Nanophysiology Group Max Planck Institute for Multidisciplinary Sciences37075 GöttingenGermany Göttingen Graduate Center for Neurosciences Biophysics and Molecular BiosciencesUniversity of Göttingen37075 GöttingenGermany Biochemistry of Membrane Dynamics Group Institute for Auditory NeuroscienceUniversity Medical Center Göttingen37075 GöttingenGermany Molecular Architecture of Synapses Group Institute for Auditory Neuroscience and InnerEarLabUniversity Medical Center Göttingen37075 GöttingenGermany Center for Biostructural Imaging of Neurodegeneration University Medical Center Göttingen37075 GöttingenGermany Auditory Systems Physiology Group Institute for Auditory Neuroscience and InnerEarLabUniversity Medical Center Göttingen37075 GöttingenGermany Departamento de Genética e Biologia Evolutiva Centro de Pesquisas sobre o Genoma Humano e Células-TroncoInstituto de BiociênciasUniversidade de São PauloSão Paulo 05508-220Brazil Laboratório de Otorrinolaringologia/LIM32 Faculdade de MedicinaHospital das ClínicasUniversidade de São PauloSão PauloSP 05508-220Brazil DERDIC Pontifícia Universidade Católica de São PauloSão Paulo 05508-220Brazil Department of Molecular Neurobiology Max Planck Institute for Multidisciplinary Sciences37075 GöttingenGermany Multiscale Bioimaging Cluster of Excellence(MBExC) University of Göttingen37075 GöttingenGermany Functional Auditory Genomics Group Auditory Neuroscience and Optogenetics LaboratoryGerman Primate Center37077 GöttingenGermany Hearing Genomics Group Institute for Auditory Neuroscience and InnerEarLabUniversity Medical Center Göttingen37075 GöttingenGermany Institute of Human Genetics University Medical Center Göttingen37075 GöttingenGermany

Dear Editor,Afferent synapses of cochlear inner hair cells(IHCs)employ a unique molecular machinery(see extended background in Supplementary Materials).Otoferlin is a key player in this machinery and its defects cause human auditory synaptopathy(Moser and Starr,2016).Otoferlin,a tail-anchored(Vogl et al.,2016)multi-C_(2)-domain protein(Fig.1Ai)specific to hair cells(Roux et al.,2006),is a member of the ferlin protein family involved in membrane trafficking and repair that are of major disease relevance(Pangršičet al.,2012),also see Supplementary *** is distributed broadly within IHCs(Fig.2Ai-Aiii;Pangrsic et al.,2010;Roux et al.,2006).

关键词： auditory hair

Dysfunction of the adhesion G protein-coupled receptor latrophilin 1(ADGRL1/LPHN1)increases the risk of obesity

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2024年第5期9卷 2298-2311页

作者： Heba ZabriTorsten Schöneberg AndréNguyen Dietzsch Hadi Al-Hasani Joachim Altschmied Katharina Bottermann Jana Brendler Judith Haendeler Susanne Horn Isabell Kaczmarek Antje Körner Kerstin Krause kathrin Landgraf Diana Le Duc Laura Lehmann Stefan Lehr Stephanie Pick Albert Ricken Rene Schnorr Angela Schulz Martina Strnadová Akhil Velluva Heba Zabri Torsten Schöneberg Doreen Thor Simone Prömel Rudolf Schönheimer Institute of Biochemistry Medical FacultyLeipzig UniversityLeipzigGermany Institute for Clinical Biochemistry and Pathobiochemistry Medical FacultyGerman Diabetes CenterLeibniz Center for Diabetes Research at Heinrich Heine University DüsseldorfDüsseldorfGermany German Center for Diabetes Research(DZD e.V.) Munich-NeuherbergGermany Cardiovascular Degeneration Clinical Chemistry and Laboratory DiagnosticsMedical FacultyUniversity Hospital and Heinrich Heine University DüsseldorfDüsseldorfGermany Cardiovascular Research Institute(CARID) Medical FacultyUniversity Hospital and Heinrich Heine University DüsseldorfDüsseldorfGermany Institute of Pharmacology Medical FacultyUniversity Hospital and Heinrich Heine University DüsseldorfDüsseldorfGermany Institute of Anatomy Medical FacultyLeipzig UniversityLeipzigGermany Center for Pediatric Research Hospital for Children and AdolescentsMedical FacultyLeipzig UniversityLeipzigGermany Helmholtz Institute for Metabolic Obesity and Vascular Research(HI-MAG)of the Helmholtz Zentrum München at the University of Leipzig and University Hospital LeipzigLeipzigGermany Department of Endocrinology NephrologyRheumatologyLeipzig University Medical CenterLeipzigGermany Institute of Human Genetics Leipzig University Medical CenterLeipzigGermany Institute of Cell Biology Department of BiologyHeinrich Heine University DüsseldorfDüsseldorfGermany School of Medicine University of Global Health EquityKigaliRwanda

Obesity is one of the diseases with severe health consequences and rapidly increasing worldwide *** the complex network of food intake and energy balance regulation is an essential prerequisite for pharmacological intervention with obesity.G protein-coupled receptors(GPCRs)are among the main modulators of metabolism and energy ***,for instance,regulate appetite and satiety in certain hypothalamic neurons,as well as glucose and lipid metabolism and hormone secretion from *** in some GPCRs,such as the melanocortin receptor type 4(MC4R),have been associated with early-onset ***,we identified the adhesion GPCR latrophilin 1(ADGRL1/LPHN1)as a member of the regulating network governing food intake and the maintenance of energy *** of the highly conserved receptor in mice results in increased food consumption and severe obesity,accompanied by dysregulation of glucose ***,we identified a partially inactivating mutation in human ADGRL1/LPHN1 in a patient suffering from ***,we propose that LPHN1 dysfunction is a risk factor for obesity development.

关键词： obesity metabolism intake

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

维生素D代谢物有助于母猪分娩过程

国外畜牧学（猪与禽） 2024年第1期44卷 12-13页

作者： Katia Pedrosa kathrin Buehler Jan Dirk Van der Klis 张相鑫(译) 王晶晶(校) 孟祥光(制图) 不详

维生素D是一种重要的营养物质,母猪在妊娠后期和哺乳期对其需要量非常高。本文概述了维生素D对母猪分娩的影响,提出可以在常规添加维生素D之外补充1,25-二羟基维生素D3糖苷,以缩短分娩时间,减少分娩过程中的人为干预,减少死胎数。

关键词：维生素D 1 25-二羟基维生素D3糖苷分娩

The contribution of maps to the challenges of risk communication to the public

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

International Journal of Digital Earth 2010年第3期3卷 292-311页

作者： Doris Dransch Henry Rotzoll kathrin Poser Helmholtz Centre Potsdam German Research Centre for GeosciencesPotsdamGermany Institute for Geography Humboldt-University BerlinBerlinGermany Center for Disaster Management and Risk Reduction Technology(CEDIM) PotsdamGermany

Risk communication is a significant challenge in risk *** serves different purposes;an important one is to improve the public risk awareness and *** of the strong spatio-temporal component of natural hazards,maps can play a decisive role in communicating risk *** application and design of maps for risk communication especially to the public has not been investigated *** constraints and challenges of risk communication have not been considered systematically in the map design process so *** study aims at developing a frame for the application and design of interactive risk and hazard maps for the public which is based on the specific constraints and challenges of risk *** a literature review it introduces concepts and methods from social sciences and psychology,which have been assessed as important for communicating risk *** concepts and methods are adapted to map-mediated risk communication according to the approaches of Activity *** objectives and tasks which are essential to improve risk mitigation are identified and geovisualization methods for information presentation are related according to the degree which they are able to serve *** on this,some principles for map-based risk communication are established.

关键词： risk management risk communication risk mitigation risk maps task-oriented geovisualization Digital Earth visualization geoinformatics GIS

Thermal desorption of mercury from lignite in a high-temperature furnace and in power plant mills

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Coal Science & Technology 2022年第5期9卷 150-159页

作者： Anne-Christin Kropp kathrin Gebauer Michael Beckmann Institute of Process Engineering and Environmental Technology Faculty of Mechanical Science and EngineeringTU Dresden01062 DresdenGermany

In this article,the binding forms of two lignite samples are determined by thermal desorption using a high-temperature *** mercury compound,such as HgCl2,has a specifc binding strength whose decomposition requires a certain thermal ***,the release of mercury from pure substances and lignite samples was analyzed in a high-temperature *** released mercury is determined with a Mercury Vapor *** obtained characteristic temperature range and peak of the mercury release were compared between lignite samples and mercury pure *** the lignite samples investigated,the binding form of mercury was then identifed as Humic *** organic compounds vaporize at lower *** half of the mercury bound in the lignite was already released at 350℃.Furthermore,the question arises whether mercury is already released during the grinding-drying process in the coal mill of a power *** two power plants,lignite samples were taken simultaneously at the feeder before entering the coal mill and at the dust line *** samples were analyzed for mercury *** results show that up to one third of the mercury was already released in the coal *** vaporized mercury enters the combustion chamber detached from the *** stated analysis methods and the results presented in this article contribute to the understanding of the mercury binding forms in *** also shows the potential of thermal coal pretreatment as a favorable alternative mercury separation technology to others such as activated carbon dosing.

关键词： Mercury binding forms Thermal desorption Mercury release Coal mill Coal pretreatment

GC-NICI-MS analysis of acetazolamide and other sulfonamide (R-SO2-NH2)drugs as pentafluorobenzyl derivatives [R-SO2-N(PFB)2] and quantification of pharmacological acetazolamide in human urine

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2020年第1期10卷 49-59页

作者： Olga Begou kathrin Drabert Georgios Theodoridis Dimitrios Tsikas Institute of Toxicology Core Unit ProteomicsHannover Medical SchoolCarl-Neuberg-Strasse 1D-30625HannoverGermany Department of Chemistry Aristotle University of Thessaloniki54124ThessalonikiGreece BIOMIC_AUTh Center for Interdisciplinary Research and Innovation(CIRI-AUTH)Balkan Center10th Km Thessaloniki-Thermi RdP.O.Box 8318GR 57001ThessalonikiGreece

Acetazolamide(molecular mass(MM),222)belongs to the class of sulfonamides(R-SO2-NH2)and is one of the strongest pharmacological inhibitors of carbonic anhydrase *** is excreted unchanged in the ***,we report on the development,validation and biomedical application of a stable-isotope dilution GC-MS method for the reliable quantitative determination of acetazolamide in human *** method is based on evaporation to dryness of 50 mL urine aliquots,base-catalyzed derivatization of acetazolamide(d0-AZM)and its internal standard[acetylo-2H3]acetazolamide(d3-AZM)in 30 vol%pentafluorobenzyl(PFB)bromide in acetonitrile(60 min,30C),reconstitution in toluene(200 mL)and injection of 1-mL *** negative-ion chemical ionization(NICI)mass spectra(methane)of the PFB derivatives contained several intense ions including[M]‒at m/z 581 for d0-AZM and m/z 584 for d3-AZM,suggesting derivatization of their sulfonamide groups to form N,N-dipentafluorobenzyl derivatives(R-SO2-N(PFB)2),i.e.,d0-AZM-(PFB)2 and d3-AZM-(PFB)2,*** was performed by selected-ion monitoring of m/z 581 and 83 for d0-AZM-(PFB)2 and m/z 584 and 86 for d3-AZM-(PFB)*** limits of detection and quantitation of the method were determined to be 300 fmol(67 pg)and 1 mM of acetazolamide,***-and inter-assay precision and accuracy for acetazolamide in human urine samples in pharmacologically relevant concentration ranges were determined to be 0.3%e4.2%and 95.3%e109%,*** method was applied to measure urinary acetazolamide excretion after ingestion of a 250 mg acetazolamide-containing tablet(Acemit®)by a healthy *** other tested sulfonamide drugs,methazolamide(MM,236)was also found to form a N,N-dipentafluorobenzyl derivative,whereas dorzolamide(MM,324)was hardly *** GC-MS peaks were obtained from the PFB bromide derivatization of hydrochlorothiazide(MM,298),xipamide(MM,355),indapamide and metholazone(MM,366 each)or br

关键词： Acetazolamide Derivatization Quantification Sulfonamides Validation

Monomer-activated Copolymerization of Ethylene Oxide and Epichlorohydrin: In Situ Kinetics Evidences Tapered Block Copolymer Formation

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2019年第9期37卷 912-918页

作者： Ann-kathrin Danner Daniel Leibig Lea-Marie Vogt Holger Frey Institute of Organic Chemistry Johannes Gutenberg- Universitat Mainz Duesbergweg 10-14 55128 Mainz Germany Graduate School Materials Science in Mainz Staudinger Weg 9 55128 Mainz Germany

The monomer-activated anionic ring-opening copolymerization (AROP) of ethylene oxide (EO) and epichlorohydrin (ECH) using tetraoctylammonium bromide as an initiator and triisobutylaluminum (i-Bu3Al) as an activator was studied. The properties of the copolymers as well as the microstructure have been analyzed in detail via an in situ NMR kinetics study. The statistical copolymers exhibited molecular weights ranging from 2350 g·mol^-1 to 38000 g·mol^-1 (measured by SEC, PEG-standards) and moderate dispersities of 1.27-1.44. The thermal property tests revealed both a glass transition and melting for all copolymers, supporting a block-like nature. Applying in situ NMR kinetic measurements, the reactivity ratios of EO and ECH were determined to be strongly disparate, i.e., rEo = 9.2 and rECH = 0.10. This shows that the simple one-pot statistical anionic copolymerization of EO and ECH via the monomer-activated AROP resulted in the formation of strongly tapered, block-like structures. Furthermore, post-polymerization functionalization of the reactive chloromethyl groups by nucleophilic displacement was investigated for the copolymers. Copolymerization of EO and ECH offers a broad platform for further functionalization and therefore the possibility to prepare a variety of multifunctional PEGs.

关键词： Epichlorohydrin Polymerization Ring-opening Copolymerization Reactivity ratios Epoxide

The C:N:P stoichiometry in bryophytes: relationships with habitat,climate and growth form

在线全文

学校读者我要写书评

暂无评论

National Science Review 2023年第4期10卷 16-18页

作者： Xin Liu Zhe Wang Xiaoming Li Weikai Bao kathrin Rousk China-Croatia ‘Belt and Road' Joint Laboratory on Biodiversity and Ecosystem Services CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province Chengdu Institute of BiologyChinese Academy of Sciences Shanghai Normal University Department of Biology Terrestrial Ecology Section University of Copenhagen

The elemental composition and stoichiometry of organisms provide a useful lens for explaining and predicting ecological paterns and processes. However, few studies have focused on stoichiometry in bryophytes, even though they are abundant in many pristine ecosystems. Several case studies found variable paterns in bryophyte stoichiometry(e.g. [1,2]).

关键词： TEMPERATURE NITROGEN

Genome and metabolic engineering in non-conventional yeasts:Current advances and applications

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2017年第3期2卷 198-207页

作者： Ann-kathrin Lobs Cory Schwartz Ian Wheeldon Department of Chemical and Environmental Engineering UC RiversideRiversideUSA

Microbial production of chemicals and proteins from biomass-derived andwaste sugar streams is a rapidly growing area of research and *** the model yeast Saccharomyces cerevisiae is an excellent host for the conversion of glucose to ethanol,production of other chemicals from alternative substrates often requires extensive strain *** avoid complex and intensive engineering of ***,other yeasts are often selected as hosts for bioprocessing based on their natural capacity to produce a desired product:for example,the efficient production and secretion of proteins,lipids,and primary metabolites that have value as commodity *** when using yeasts with beneficial native phenotypes,metabolic engineering to increase yield,titer,and production rate is *** non-conventional yeasts Kluyveromyces lactis,***,Scheffersomyces stipitis,Yarrowia lipolytica,Hansenula polymorpha and Pichia pastoris have been developed as eukaryotic hosts because of their desirable phenotypes,including thermotolerance,assimilation of diverse carbon sources,and high protein ***,advanced metabolic engineering in these yeasts has been *** review outlines the challenges of using non-conventional yeasts for strain and pathway engineering,and discusses the developed solutions to these problems and the resulting applications in industrial biotechnology.

关键词： CRISPR-Cas9 Kluyveromyces lactis Kluyveromyces marxianus Scheffersomyces stipitis Yarrowia lipolytica Hansenula polymorpha Pichia pastoris