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Copper as an alternative antimicrobial coating for implants-An in vitro study

World Journal of Transplantation 2017年第3期7卷 193-202页

作者： Claudia Bergemann Sarah Zaatreh katharina wegner Kathleen Arndt Andreas Podbielski Rainer Bader Cornelia Prinz Ulrich Lembke J Barbara Nebe Department of Cell Biology University Medical Center Rostock Biomechanics and Implant Technology Research Laboratory University Medical Center Rostock Institute of Medical Microbiology Virology and HygieneUniversity Medical Center Rostock DOT GmbH

AIM To investigate osteoconductive and antimicrobial properties of a titanium-copper-nitride(TiC uN) film and an additional BONIT~? coating on titanium *** For micro-structuring, the surface of titanium test samples was modified by titanium plasma spray(TPS). On the TPS-coated samples, the Ti Cu N layer was deposited by physical vapor deposition. The BONIT~? layer was coated electrochemically. The concentration of copper ions released from TiC uN films was measured by atomic absorption spectrometry. MG-63 osteoblasts on Ti Cu N and BONIT~? were analyzed for cell adhesion, viability and spreading. In parallel, Staphylococcus epidermidis(S. epidermidis) were cultivated on the samples and planktonic and biofilm-bound bacteria were quantified bycounting of the colony-forming units. RESULTS Field emission scanning electron microscopy(FESEM) revealed rough surfaces for TPS and TiC uN and a special crystalline surface structure on TiC uN + BONIT~?. TiC uN released high amounts of copper quickly within 24 h. These release dynamics were accompanied by complete growth inhibition of bacteria and after 2 d, no planktonic or adherent S. epidermidis were found on these samples. On the other hand viability of MG-63 cells was impaired during direct cultivation on the samples within 24 h. However, high cell colonization could be found after a 24 h pre-incubation step in cell culture medium simulating the in vivo dynamics closer. On pre-incubated TiC uN, the osteoblasts span the ridges and demonstrate a flattened, well-spread phenotype. The additional BONIT~?-coating reduced the copper release of the TiC uN layer significantly and showed a positive effect on the initial cell *** The Ti Cu N-coating inhibits the formation of bacterial biofilms on orthopedic implants by influencing the "race for the surface" to the advantage of osteoblasts.

关键词： Implant-coating Antimicrobial effect Titanium plasma spray Titanium-copper-nitride BONIT^(®) Osteoconductivity

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