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Engineering 2023年第7期26卷 32-43,I0002页

作者： Di Wang katarina madunic Tao Zhang Guinevere S.M.Lageveen-Kammeijer Manfred Wuhrer Center for Proteomics and Metabolomics Leiden University Medical CenterLeiden 2300 RCNetherlands Copenhagen Center for Glycomics Department of Cellular and Molecular MedicineUniversity of CopenhagenCopenhagen 2200Denmark

Aberrant glycosylation is considered to be a hallmark of colorectal cancer(CRC),as demonstrated by various *** the N-glycosylation of cell lines and serum has been widely examined,the analysis of cancer-associated N-glycans from tissues has been hampered by the heterogeneity of tumors and the complexity of N-glycan *** overcome these obstacles,we present a study using laser capture microdissection that makes it possible to largely deconvolute distinct N-glycomic signatures originating from different regions of heterogeneous tissues including cancerous,stromal,and healthy mucosa cells.N-glycan alditols were analyzed by means of porous graphitized carbon liquid chromatographyelectrospray ionization tandem mass spectrometry,enabling the differentiation and structural characterization of isomeric *** total,116 N-glycans were identified that showed profound differences in expression among cancer,stroma,and normal *** comparison with healthy mucosa,the cancer cells showed an increase in a2-6 sialylation and monoantennary N-glycans,as well as a decrease in bisected ***,specific sialylated and(sialyl-)LewisA/X antigen-carrying N-glycans were exclusively expressed in *** comparison with cancer,the stroma showed lower levels of oligomannosidic and monoantennary N-glycans,LewisA/X epitopes,and sulfation,as well as increased expression of(core-)fucosylation and a2-3 *** study reveals the distinct N-glycomic profiles of different cell types in CRC and control tissues,proving the necessity of their separate analysis for the discovery of cancer-associated glycans.

关键词： Colorectal cancer Tumor Porous graphitized carbon liquid chromatography mass spectrometry N-glycomics Antibody response

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