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An UPLC-MS/MS method for simultaneous determination of multiple constituents in Guizhi Fuling capsule with ultrafast positive/negative ionization switching

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Chinese Journal of Natural Medicines 2018年第4期16卷 313-320页

作者： ZHANG Rong-Hua LI Chao-Ran YANG Hua LI Meng-Ning karl w.k.tsim LI Ping GAO wen State key Laboratory of Natural Medicines School of Traditional Chinese Pharmacy China Pharmaceutical University School of Traditional Chinese Medicine Guangdong Pharmaceutical University Division of Life Science and Center for Chinese Medicine The Hong Kong University of Science and Technology

Guizhi Fuling capsule(GFC), a traditional Chinese medicine(TCM) with effects of promoting blood circulation and dissipating blood stasis, has been widely used in the clinic. Because of the complex matrix and various chemical structure types, quality control of GFC remains great challenge. In the present study, an ultra performance liquid chromatography hybrid triple-quadrupole mass spectrometry(UPLC-QQQ MS) method with ultrafast positive/negative ionization switching was developed for simultaneous determination of 18 bioactive components in GFC, including methyl gallate, ethyl gallate, oxypaeoniflorin, benzoic acid, albiflorin, paeonolide, paeoniflorin, 1, 2, 3, 4, 6-pentagalloylglucose, mudanpioside C, benzoyloxypaeoniflorin, benzoylpaeoniflorin, pachymic acid, amygdalin, cinnamaldehyde, paeonol, cinnamic acid, 4-hydroxybenzoic acid, and gallic acid. Separation was performed on an Agilent Zorbax Extend–C18 column(2.1 mm × 50 mm, 1.8 μm), using a gradient elution with acetonitrile and water containing 0.1% formic acid. Cholic acid was selected as the internal standard. This newly developed method was fully validated for linearity, precision, accuracy, and stability, and then applied to quality assessment of GFC. Finally, the batch-to-batch reproducibility of GFC samples was evaluated by the cosine ration and Euclidean distance method, which showed high quality consistency. The results demonstrated that the developed method provided a reasonable and powerful manner for quality control of GFC.

关键词： Guizhi Fuling capsule(GFC) UPLC-QQQ MS Ultrafast positive/negative ionization switching Quality control

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The biodiversity of flavonoids from Chinese medicines-development of health food supplements for health promotion and disease prevention

The biodiversity of flavonoids from Chinese medicines-develo...

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中国化学会第十届全国天然有机化学学术会议

作者： karl w.k.tsim Hong kong University of Science ＆ Technology

＜正＞Despite the classical hormonal effect,estrogen possesses neuroprotective effect in the brain,which leads to search for novel treatments for neurodegenerative ***,a group of compounds mainly derived from vegetables,share a resemblance,chemically,to estrogen,and indeed some of which have been used as estrogen *** order to search for potential

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Interacting with α7 nAChR is a new mechanism for AChE to enhance the inflammatory response in macrophages

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Acta Pharmaceutica Sinica B 2020年第10期10卷 1926-1942,2020页

作者： Etta Y.L.Liu Yingjie Xia Xiangpeng kong Maggie S.S.Guo Anna X.D.Yu Brody Z.Y.Zheng Shinghung Mak Miranda L.Xu karl w.k.tsim key Laboratory of Food Quality and Safety of Guangdong Province College of Food ScienceSouth China Agricultural UniversityGuangzhou 510642China Shenzhen key Laboratory of Edible and Medicinal Bioresources the Hong Kong University of Science and TechnologyShenzhen 518057China Division of Life Science Center for Chinese Medicinethe Hong Kong University of Science and TechnologyHong kongChina

Acetylcholine(ACh)regulates inflammation viaα7 nicotinic acetylcholine receptor(α7 nAChR).Acetylcholinesterase(AChE),an enzyme hydrolyzing ACh,is expressed in immune cells suggesting non-classical function in inflammatory ***,the expression of PRiMA-linked G4 AChE was identified on the surface of *** lipopolysaccharide-induced inflammatory processes,AChE was upregulated by the binding of NF-κB onto the ACHE ***,the overexpression of G4 AChE inhibited ACh-suppressed cytokine release and cell migration,which was in contrast to that of applied AChE ***,a DNA construct without enzymatic activity,was adopted to identify the protein role of AChE in immune *** of G4 AChEmt induced cell migration and inhibited ACh-suppressed cell *** co-localization ofα7 nAChR and AChE was found in macrophases,suggesting the potential interaction ofα7 nAChR and ***,immunoprecipitation showed a close association ofα7 nAChR and AChE protein in cell ***,the novel function of AChE in macrophage by interacting withα7 nAChR was *** with hydrolysis of ACh,AChE plays a direct role in the regulation of inflammatory *** such,AChE could serve as a novel target to treat age-related diseases by antiinflammatory responses.

关键词： Macrophage AChE Cholinergic anti-inflammatory pathway α7 nAChR

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Luteolin， a common flavone in vegetables， acts as a hormetin possessing neurotrophic function: signaling mediated by inducing mitochondrial stress

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Food Science and Human wellness 2024年

作者： Alex Xiong Gao Tracy Chenxi Xia Maggie Suisui Guo Gary kawing Yuen kevin Qiyun wu Tina Tingxia Dong karl wah-keung tsim Shenzhen key Laboratory of Edible and Medicinal Bioresources HkUST Shenzhen Research Institute Division of Life Science and Center for Chinese Medicine The Hong kong University of Science and Technology

This study aims to uncover the hormetic process of luteolin， a common dietary flavone， in neuronal cells through its role in inducing mitochondrial stress. In rat pheochromocytoma PC12 cells， luteolin at low concentrations caused a mild and reversible loss of mitochondrial membrane potential (MMP)， while high concentrations of luteolin triggered intense and sustained depolarization of MMP. The MMP disturbance was shown to have a close association with the trophic and/or toxic effects triggered by luteolin; because the common mitochondrial uncouplers shared similar bi-phase dose-response on cell viability， as that of luteolin. Along with the induced MMP pertubation， luteolin triggered the development of autophagy and mitophagy， as determined by mCherry-GFP-LC3B tandem protein， and by the co-localization of mitochondrial/lysosomal staining. Subsequent application of autophagy inhibitors in the cultures blocked the luteolin-induced neurotrophic activities and sensitized the cells to be less resistant to luteolin-mediated cytotoxicity. Other flavonoids also displayed similar properties in the cultures， indicating that these compounds act as hormetic pharmacological inducers that stimulate cells to become more resilient and adapt to threats. This study provides a unifying mechanistic explanation for the neuro-beneficial effects of luteolin and other flavonoids.

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Single-atom catalysts for the electrochemical reduction of carbon dioxide into hydrocarbons and oxygenates

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Carbon Energy 2024年第3期6卷 64-117页

作者： karl Adrian Gandionco Juwon kim Lieven Bekaert Annick Hubin Jongwoo Lim Department of Chemistry Seoul National UniversitySeoulRepublic of Korea Research Group Electrochemical and Surface Engineering(SURF) Department of Materials and ChemistryVrije Universiteit BrusselBrusselsBelgium

The electrochemical reduction of carbon dioxide offers a sound and economically viable technology for the electrification and decarbonization of the chemical and fuel *** this technology,an electrocatalytic material and renewable energy-generated electricity drive the conversion of carbon dioxide into high-value chemicals and carbon-neutral *** the past few years,single-atom catalysts have been intensively studied as they could provide near-unity atom utilization and unique catalytic ***-atom catalysts have become one of the state-of-the-art catalyst materials for the electrochemical reduction of carbon dioxide into carbon ***,it remains a challenge for single-atom catalysts to facilitate the efficient conversion of carbon dioxide into products beyond carbon *** this review,we summarize and present important findings and critical insights from studies on the electrochemical carbon dioxide reduction reaction into hydrocarbons and oxygenates using single-atom *** is hoped that this review gives a thorough recapitulation and analysis of the science behind the catalysis of carbon dioxide into more reduced products through singleatom catalysts so that it can be a guide for future research and development on catalysts with industry-ready performance for the electrochemical reduction of carbon dioxide into high-value chemicals and carbon-neutral fuels.

关键词： electrocatalysis electrochemical CO_(2)reduction hydrocarbons oxygenates single-atom catalysts

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天然与发酵培养冬虫夏草中核苷类成分的含量及其变化(英文)

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Journal of Chinese Pharmaceutical Sciences 2001年第4期10卷 175-179页

作者：李绍平李萍季晖朱荃董婷霞詹华强中国药科大学南京中医药大学南京210029 南京中医药大学香港科技大学生物技术研究所

目的比较天然和人工冬虫夏草中核苷类成分的含量,并对其变化进行考察。方法采用高效毛细管电泳法测定天然和人工冬虫夏草中核苷类成分的含量;考察湿热对冬虫夏草中核苷类成分含量的影响。结果人工冬虫夏草的核苷含量明显高于天然冬... 详细信息

目的比较天然和人工冬虫夏草中核苷类成分的含量,并对其变化进行考察。方法采用高效毛细管电泳法测定天然和人工冬虫夏草中核苷类成分的含量;考察湿热对冬虫夏草中核苷类成分含量的影响。结果人工冬虫夏草的核苷含量明显高于天然冬虫夏草;新采集的天然冬虫夏草中核苷类物质含量极低,而采集日久者核苷类成分含量却较高;湿热可明显增加天然冬虫夏草中核苷类物质的含量,但对人工冬虫夏草中核苷类成分的含量无明显影响。结论天然和人工冬虫夏草中核苷类成分存在一定差异,天然冬虫夏草中核苷类物质可能主要来源于大分子核苷(酸)的降解,腺苷在天然冬虫夏草质量控制中的应用价值尚值得进一步研究。

关键词：核苷类成分冬虫夏草天然含量人工湿热高效毛细管电泳法发酵培养采集应用价值

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Superior antibody and membrane protein‑specific T‑cell responses to CoronaVac by intradermal versus intramuscular routes in adolescents

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world Journal of Pediatrics 2024年第4期20卷 353-370页

作者： Jaime S.Rosa Duque Samuel M.S.Cheng Carolyn A.Cohen Daniel Leung Xiwei wang Xiaofeng Mu Yuet Chung Tsun Ming Lau Manni wang wenyue Zhang Yanmei Zhang Howard H.w.wong Leo C.H.Tsang Sara Chaothai Tsz Chun kwan John k.C.Li karl C.k.Chan Leo L.H.Luk Jenson C.H.Ho wing Yan Li Amos M.T.Lee Jennifer H.Y.Lam Sau Man Chan wilfred H.S.wong Issan Y.S.Tam Masashi Mori Sophie A.Valkenburg Malik Peiris wenwei Tu Yu Lung Lau Department of Paediatrics and Adolescent Medicine The University of Hong KongHong KongChina School of Public Health The University of Hong KongHong KongChina HkU‑Pasteur Research Pole School of Public HealthThe University of Hong KongHong KongChina Research Institute for Bioresources and Biotechnology Ishikawa Prefectural UniversityNonoichiJapan Department of Microbiology and Immunology Peter Doherty Institute for Infectionand ImmunityUniversity of MelbourneMelbourneVICAustralia Center for Immunology and Infection C2i Hong KongChina

Background Optimising the immunogenicity of COVID-19 vaccines to improve their protection against disease is *** dosing by intradermal(ID)administration has been shown to be equally immunogenic as intramuscular(IM)administration for several vaccines,but the immunogenicity of ID inactivated whole severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)at the full dose is *** study(NCT04800133)investigated the superiority of antibody and T-cell responses of full-dose CoronaVac by ID over IM administration in *** Participants aged 11–17 years received two doses of IM or ID vaccine,followed by the 3rd dose 13–42 days *** and cellular immunogenicity outcomes were measured post-dose 2(IM-CC versus ID-CC)and post-dose 3(IMCCC versus ID-CCC).Doses 2 and 3 were administered to 173 and 104 adolescents,*** Spike protein(S)immunoglobulin G(IgG),S-receptor-binding domain(RBD)IgG,S IgG Fcγreceptor IIIa(FcγRIIIa)-binding,SNM[sum of individual(S),nucleocapsid protein(N),and membrane protein(M)peptide pool]-specific interleukin-2(IL-2)+CD4^(+),SNM-specific IL-2^(+)CD8^(+),S-specific IL-2^(+)CD8^(+),N-specific IL-2^(+)CD4^(+),N-specific IL-2^(+)CD8^(+)and M-specific IL-2^(+)CD4^(+)responses fulfilled the superior and non-inferior criteria for ID-CC compared to IM-CC,whereas IgG avidity was *** ID-CCC,S-RBD IgG,surrogate virus neutralisation test,90%plaque reduction neutralisation titre(PRNT90),PRNT50,S IgG avidity,S IgG FcγRIIIa-binding,M-specific IL-2^(+)CD4^(+),interferon-γ+CD8^(+)and IL-2^(+)CD8^(+)responses were superior and non-inferior to *** estimated vaccine efficacies were 49%,52%,66%and 79%for IM-CC,ID-CC,IM-CCC and ID-CCC,*** ID groups reported more local,mild adverse *** This is the first study to demonstrate superior antibody and M-specific T-cell responses by ID inactivated SARSCoV-2 vaccination and serves as the basis for future research to improve the

关键词： CoronaVac Coronavirus disease 2019 Immunity Intradermal Vaccine

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Elucidating regulatory processes of intense physical activity by multi-omics analysis

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Military Medical Research 2024年第4期 479-499页

作者： Ernesto S.Nakayasu Marina A.Gritsenko Young-Mo kim Jennifer E.kyle kelly G.Stratton Carrie D.Nicora Nathalie Munoz kathleen M. Navarro Daniel Claborne Yuqian Gao karl k.weitz Vanessa L. Paurus kent J. Bloodsworth kelsey A. Allen Lisa M. Bramer Fernando Montes kathleen A. Clark Grant Tietje Justin Teeguarden kristin E.Burnum-Johnson Biological Sciences Division Pacific Northwest National Laboratory Environmental and Molecular Sciences Division Pacific Northwest National Laboratory Centers for Disease Control and Prevention National Institute for Occupational Safety and Health Western States Division Computational Analytics Division Pacific Northwest National Laboratory National Security Directorate Pacific Northwest National Laboratory Los Angeles County Fire Department Centers for Disease Control and Prevention National Institute for Occupational Safety and Health Respiratory Health Division Environmental and Molecular Toxicology Oregon State University

Background: Physiological and biochemical processes across tissues of the body are regulated in response to the high demands of intense physical activity in several occupations, such as firefighting, law enforcement, military, and sports. A better understanding of such processes can ultimately help improve human performance and prevent illnesses in the work ***: To study regulatory processes in intense physical activity simulating real-life conditions, we performed a multi-omics analysis of 3 biofluids(blood plasma, urine, and saliva) collected from 11 wildland firefighters before and after a 45 min, intense exercise regimen. Omics profiles post-vs. pre-exercise were compared by Student's t-test followed by pathway analysis and comparison between the different omics ***: Our multi-omics analysis identified and quantified 3835 proteins, 730 lipids and 182 metabolites combining the 3 different types of samples. The blood plasma analysis revealed signatures of tissue damage and acute repair response accompanied by enhanced carbon metabolism to meet energy demands. The urine analysis showed a strong, concomitant regulation of 6 out of 8 identified proteins from the renin-angiotensin system supporting increased excretion of catabolites, reabsorption of nutrients and maintenance of fluid balance. In saliva, we observed a decrease in 3 pro-inflammatory cytokines and an increase in 8 antimicrobial peptides. A systematic literature review identified 6 papers that support an altered susceptibility to respiratory ***: This study shows simultaneous regulatory signatures in biofluids indicative of homeostatic maintenance during intense physical activity with possible effects on increased infection susceptibility, suggesting that caution against respiratory diseases could benefit workers on highly physical demanding jobs.

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Synthesis and Anti-inflammatory Activity of a Novel Series of 9,10-Dihydro-4H,8H-chromeno[8,7-e][1,3]oxazin-4-one Derivatives

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Chemical Research in Chinese Universities 2010年第2期26卷 268-271页

作者： LIU Xiao-ping wANG Ying LAN Hui-yu SONG Ai-hua tsim karl wah-keung DONG Tina Ting-xia HU Chun Department of Organic Chemistry School of Pharmaceutical Engineering Shenyang Pharmaceutical University Shenyang 110016 P. R. China Tianjin key Laboratory of Chemistry and Analysis of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine Tianjin 300193 P. R. China Department of Biology The Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong P. R. China

Flavonoids exhibit a wide range of biological activities including anti-inflammatory activity, while some 1,3-benzoxazine derivatives show anti-inflammatory activity. In order to explore the novel anti-inflammatory agents, based on the principles of bioisosterism and hybridization, ten novel ehromeno[8,7-e][1,3]oxazin-4-ones were syn- thesized and characterized with IR, ^1H NMR, MS and elemental analyses. The anti-inflammatory activities of the target compounds were evaluated via the croton oil-induced ear oedema test in Swiss mice. According to screened resuits, some target compounds show potent anti-inflammatory activity.

关键词： Anti-inflammatory activity Heterocycle Flavone Chromeno[8,7-e]oxazin-4-one

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Rare Earths and Magnetic Refrigeration

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Journal of Rare Earths 2006年第6期24卷 641-647页

作者： karl A Gschneidner Vitalij k Pecharsky Materials and Engineering Physics Program Ames Laboratory and Department of Materials Science and Engineering Iowa State University Ames Iowa USAJr. Materials and Engineering Physics Program Ames Laboratory and Department of Materials Science and Engineering Iowa State University Ames Iowa USA

Magnetic refrigeration is a revolutionary, efficient, environmentally friendly cooling technology, which is on the threshold of commercialization. The magnetic rare earth materials are utilized as the magnetic refrigerants in most cooling devices, and for many cooling application the Nd2Fe14B permanent magnets are employed as the source of the magnetic field. The status of the near room temperature magnetic cooling was reviewed.

关键词： magnetic refrigeration magnetocaloric effect gadolinium Gd5 ( Si1- x Gex ) 4 La ( Fe 13 - x Six ) Hy Nd2 Fe14 B permanent magnets active magnetic regenerator cycle rare earths

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