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Molecular phenotypes of human parvovirus B19 in patients with myocarditis

World Journal of Cardiology 2014年第4期6卷 183-195页

作者： C-Thomas Bock Anja Düchting Friederike Utta Eva Brunner Bui Tien Sy karin klingel Florian Lang Meinrad Gawaz Stephan B Felix Reinhard Kandolf Department of Molecular Pathology University Hospital of Tuebingen Robert Koch Institute Department of Physiology University of Tuebingen Department of Cardiology and Cardiovascular Medicine University Hospital of Tuebingen Clinics for Internal Medicine B Ernst-MoritzArndt-University Department of Molecular Pathology University Hospital of Tuebinge

AIM:To investigate molecular phenotypes of myocardial B19V-infection to determine the role of B19V in myocarditis and dilated cardiomyopathy(DCM).METHODS:Endomyocardial biopsies(EMBs) from 498 B19V-positive patients with myocarditis and DCMwere analyzed using molecular methods and functional *** were obtained from the University Hospitals of Greifswald and Tuebingen and additionally from 36 German cardiology *** tissues were obtained at autopsy from 34 victims of accidents,crime or *** of mononuclear cell infiltrates in EMBs was performed using immunohistological ***-B19V-IgM and anti-B19V-IgG were analyzed by enzyme-linked immunosorbent assay(ELISA).B19V viral loads were determined using in-house quantitative real-time polymerase chain reaction(PCR).For B19V-genotyping a new B19V-genotype-specific restriction fragment length polymorphism(RFLP)-PCR was established.B19V-genotyping was verified by direct DNAsequencing and sequences were aligned using BLAST and BioEdit software.B19V P6-promoter and HHV6-U94-transactivator constructs were generated for cell culture *** experiments were conducted using human endothelial cells *** reporter assays were performed to determine B19Vreplication *** analysis and graphical representation were calculated using SPSS and Prism5 ***:The prevalence of B19V was significantly more likely to be associated with inflammatory cardiomyopathy(iCMP) compared to uninflamed DCM(59.6% vs 35.3%)(P < 0.0001).The detection of B19V-mRNA replication intermediates proved that replication of B19V was ***-PCR assays showed that B19V-genotype 1(57.4%) and B19V-genotype 2(36.7%) were the most prevalent viral genotypes.B19V-genotype 2 was observed more frequently in EMBs with iCMP(65.0%) compared to DCM(35%)(P = 0.049).Although there was no significant difference in gender-specific B19V-loads,women were more frequently infected with B1

关键词： Myocarditis Dilated cardiomyopathy Parvovirus B19 B19V-genotypes B19V co-infection

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