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Two VOZ transcription factors link an E3 ligase and an NLR immune receptor to modulate immunity in rice

Molecular Plant 2021年第2期14卷 253-266页

作者： Jiyang Wang Ruyi Wang Hong Fang Chongyang Zhang Fan Zhang Zeyun Hao Xiaoman You Xuetao Shi Chan Ho Park kangyu hua Feng He Maria Bellizzi Kieu Thi Xuan Vo Jong-Seong Jeon Yuese Ning Guo-Liang Wang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Department of Plant Pathology Ohio State UniversityColumbusOH 43210USA Graduate School of Biotechnology and Crop Biotech Institute Kyung Hee UniversityYongin 17104Korea

Nucleotide-binding leucine-rich repeat(NLR)proteins play critical roles in plant ***,how NLRs are regulated and activate defense signaling is not fully *** rice(Oryza sativa)NLR receptor Piz-t confers broad-spectrum resistance to the fungal pathogen Magnaporthe oryzae and the RING-type E3 ligase AVRPIZ-T INTERACTING PROTEIN 10(APIP10)negatively regulates Piz-t *** this study,we found that APIP10 interacts with two rice transcription factors,VASCULAR PLANT ONEZINC FINGER 1(OsVOZ1)and OsVOZ2,and promotes their degradation through the 26S proteasome ***1 displays transcriptional repression activity while OsVOZ2 confers transcriptional activation activity in *** osvoz1 and osvoz2 single mutants display modest but opposite *** resistance in the non-Piz-t ***,the osvoz1 osvoz2 double mutant exhibits strong dwarfism and cell death,and silencing of both genes via RNA interference also leads to dwarfism,mild cell death,and enhanced resistance to *** in the non-Piz-t *** OsVOZ1 and OsVOZ2 interact with *** silencing of OsVOZ1 and OsVOZ2 in the Piz-t background decreases Piz-t protein accumulation and transcription,reactive oxygen species-dependent cell death,and resistance to *** containing *** together,these results indicate that OsVOZ1 and OsVOZ2 negatively regulate basal defense but contribute positively to Piz-t-mediated immunity.

关键词： rice E3 ligase VOZ transcription factor NLR protein fungal pathogen Magnaporthe oryzae cell death

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Rapid Detection of Wheat Blast Pathogen Magnaporthe oryzae Triticum Pathotype Using Genome-Specific Primers and Cas12a-mediated Technology

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Engineering 2021年第9期7卷 1326-1335页

作者： Houxiang Kang Ye Peng kangyu hua Yufei Deng Maria Bellizzi Dipali Rani Gupta Nur Uddin Mahmud Alfredo S.Urashima Sanjoy Kumar Paul Gary Peterson Yilin Zhou Xueping Zhou Md Tofazzal Islam Guo-Liang Wang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Department of Plant Pathology The Ohio State UniversityColumbusOH 43210USA Institute of Biotechnology and Genetic Engineering(IBGE) Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityDhaka 1706Bangladesh Centro de Ciências Agrárias Universidade Federal de São CarlosArarasSP CEP 13600-000Brazil Foreign Disease-Weed Science Research Unit(FDWSRU) United States Department of Agriculture-Agricultural Research Service(USDA-ARS)Ft.DetrickMD 21702USA

Wheat blast,caused by the fungus Magnaporthe oryzae Triticum(MoT)pathotype,is a devastating disease persistent in South America and *** MoT generally fails to cause visual symptoms in wheat until the heading stage when the infection would have advanced,disease control by fungicide application solely based on the detection of visual symptoms is *** develop an accurate and sensitive method to detect MoT at the seedling and vegetative stages for disease control,we sequenced the genomes of two MoT isolates from Brazil and identified two DNA fragments,MoT-6098 and MoT-6099,that are present in the MoT genome but not in the genome of the rice-infecting Magnaporthe oryzae Oryzae(MoO)*** polymerase chain reaction(PCR),we confirmed the specificity of the two markers in 53 MoT and MoO isolates from South America and *** test the efficiency of the two markers,we first established a loop-mediated isothermal amplification(LAMP)method to detect MoT at isothermal conditions,without the use of a PCR *** this,we used the Cas12a protein and guide RNAs(gRNAs)to target the MoT-6098 and MoT-6099 *** activated Cas12a showed indiscriminate single-stranded deoxyribonuclease(ssDNase)*** then combined targetdependent Cas12a ssDNase activation with recombinase polymerase amplification(RPA)and nucleic acid lateral flow immunoassay(NALFIA)to develop a method that accurately,sensitively,and cost-effectively detects MoT-specific DNA sequences in infected wheat *** novel technique can be easily adapted for the rapid detection of wheat blast and other important plant diseases in the field.

关键词： Wheat blast Magnaporthe oryzae Triticum Cas12a Nucleic acid rapid lateral flow immunoassay Field detection

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On the Pathwise Uniqueness of Solutions of One-dimensional Reflected Stochastic Differential Equations with Jumps

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Acta Mathematicae Applicatae Sinica 2024年第1期40卷 149-163页

作者： hua Zhang School of Statistics and Data Science&Key Laboratory of Data Science in Finance and Economics Jiangxi University of Finance and EconomicsNanchang330013China

In this paper, we are concerned with the problem of the pathwise uniqueness of one-dimensional reflected stochastic differential equations with jumps under the assumption of non-Lipschitz continuous coefficients whose... 详细信息

关键词： reflected diffusion processes with jumps pathwise uniqueness local time Meyer It?'s formula

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Goal-Oriented Control Systems(GOCS):From HOW to WHAT

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IEEE/CAA Journal of Automatica Sinica 2024年第4期11卷 816-819页

作者： Wen-hua Chen the Department of Aeronautical and Automotive Engineering Loughborough UniversityLeicestershireLE113TUU.K.

Brief:New control theory is required to underpin safe design and deployment of future highly automated systems to deal with uncertain environments and complicated tasks,enabled by AI and other advanced ***-Oriented Control Systems offer potential to transform the control system design from currently instructing a control system how to perform a task to specifying what is to be achieved.

关键词： automated instru system

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Cardiovascular computed tomography in cardiovascular disease:An overview of its applications from diagnosis to prediction

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Journal of Geriatric Cardiology 2024年第5期21卷 550-576页

作者： Zhong-hua SUN Discipline of Medical Radiation Science Curtin Medical SchoolCurtin UniversityPerthAustralia Curtin Health Innovation Research Institute(CHIRI) Curtin UniversityPerth 6012Australia

Cardiovascular computed tomography angiography(CTA)is a widely used imaging modality in the diagnosis of cardiovascular *** in CT imaging technology have further advanced its applications from high diagnostic value to minimising radiation exposure to *** addition to the standard application of assessing vascular lumen changes,CTA-derived applications including 3D printed personalised models,3D visualisations such as virtual endoscopy,virtual reality,augmented reality and mixed reality,as well as CT-derived hemodynamic flow analysis and fractional flow reserve(FFRCT)greatly enhance the diagnostic performance of CTA in cardiovascular *** widespread application of artificial intelligence in medicine also significantly contributes to the clinical value of CTA in cardiovascular *** value of CTA has extended from the initial diagnosis to identification of vulnerable lesions,and prediction of disease extent,hence improving patient care and *** this review article,as an active researcher in cardiovascular imaging for more than 20 years,I will provide an overview of cardiovascular CTA in cardiovascular *** is expected that this review will provide readers with an update of CTA applications,from the initial lumen assessment to recent developments utilising latest novel imaging and visualisation *** will serve as a useful resource for researchers and clinicians to judiciously use the cardiovascular CT in clinical practice.

关键词： diagnosis cardiovascular prediction

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Reliability of a Multicomponent Stress-strength Model Based on a Bivariate Kumaraswamy Distribution with Censored Data

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Acta Mathematicae Applicatae Sinica 2024年第2期40卷 478-507页

作者： Cong-hua CHENG School of Mathematics and Statistics Zhaoqing UniversityZhaoqing 526061China

In this paper,we consider a system which has k statistically independent and identically distributed strength components and each component is constructed by a pair of statistically dependent elements with doubly type-II censored *** elements(X1,Y1),(X2,Y2),…,(Xk,Yk)follow a bivariate Kumaraswamy distribution and each element is exposed to a common random stress T which follows a Kumaraswamy *** system is regarded as operating only if at least s out of k(1≤s≤k)strength variables exceed the random *** multicomponent reliability of the system is given by Rs,k=P(at least s of the(Z1,…,Zk)exceed T)where Zi=min(Xi,Yi),i=1,…,*** Bayes estimates of Rs,k have been developed by using the Markov Chain Monte Carlo methods due to the lack of explicit *** uniformly minimum variance unbiased and exact Bayes estimates of Rs,k are obtained analytically when the common second shape parameter is *** asymptotic confidence interval and the highest probability density credible interval are constructed for Rs,*** reliability estimators are compared by using the estimated risks through Monte Carlo simulations.

关键词： stress-strength model bivariate Kumaraswamy distribution multicomponent reliability doubly Type-II censored scheme interval estimation

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Nuclear charge radius predictions by kernel ridge regression with odd-even effects

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Nuclear Science and Techniques 2024年第2期35卷 94-102页

作者： Lu Tang Zhen-hua Zhang Mathematics and Physics Department North China Electric Power UniversityBeijing 102206China Hebei Key Laboratory of Physics and Energy Technology North China Electric Power UniversityBaoding 071000China

The extended kernel ridge regression(EKRR)method with odd-even effects was adopted to improve the description of the nuclear charge radius using five commonly used nuclear *** are:(i)the isospin-dependent A^(1∕3) formula,(ii)relativistic continuum Hartree-Bogoliubov(RCHB)theory,(iii)Hartree-Fock-Bogoliubov(HFB)model HFB25,(iv)the Weizsacker-Skyrme(WS)model WS*,and(v)HFB25**** the last two models,the charge radii were calculated using a five-parameter formula with the nuclear shell corrections and deformations obtained from the WS and HFB25 models,*** each model,the resultant root-mean-square deviation for the 1014 nuclei with proton number Z≥8 can be significantly reduced to 0.009-0.013 fm after considering the modification with the EKRR *** best among them was the RCHB model,with a root-mean-square deviation of 0.0092 *** extrapolation abilities of the KRR and EKRR methods for the neutron-rich region were examined,and it was found that after considering the odd-even effects,the extrapolation power was improved compared with that of the original KRR *** strong odd-even staggering of nuclear charge radii of Ca and Cu isotopes and the abrupt kinks across the neutron N=126 and 82 shell closures were also calculated and could be reproduced quite well by calculations using the EKRR method.

关键词： Nuclear charge radius Machine learning Kernel ridge regression method

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Impacts of external forcing and internal variability on the North Atlantic multidecadal variations since 1950

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Science China Earth Sciences 2024年第8期67卷 2699-2701页

作者： Wenjian hua Aiguo DAI Key Laboratory of Meteorological Disaster Ministry of Education/Collaborative Innovation Center on Forecast and Evaluation of Meteorological DisastersNanjing University of Information Science and TechnologyNanjing 210044China Department of Atmospheric and Environmental Sciences University at AlbanyState University of New YorkAlbany 12222USA

The physical formation mechanisms of the Atlantic Multidecadal Variability(AMV)in the context of global warming have been a key research topic(Vecchi et al.,2017).The conventional view is that the AMV is mainly driven by internal dynamics within the climate system,especially in the Atlantic Ocean(Zhang et al.,2019).However,some studies suggest that decadal to multidecadal variations in North Atlantic sea surface temperature(SST)since around 1950 are related to anthropogenic and natural aerosol emissions(Otter?et al.,2010;Booth et al.,2012;hua et al.,2019).

关键词： Atlantic decadal forcing

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A novel weld-pool-length monitoring method based on pixel analysis in plasma arc additive manufacturing

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Advances in Manufacturing 2024年第2期12卷 335-348页

作者： Bao-Ri Zhang Yong-hua Shi Bionic Robot Research Department Ji Hua LaboratoryFoshan528200GuangdongPeople’s Republic of China Guangdong Provincial Engineering Research Center for Special Welding Technology and Equipment School of Mechanical and Automotive EngineeringSouth China University of TechnologyGuangzhou510640People’s Republic of China

The real-time monitoring of the weld pool during deposition is important for automatic control in plasma arc additive *** obtain a high deposition accuracy,it is essential to maintain a stable weld pool *** this study,a novel passive visual method is proposed to measure the weld pool *** the proposed method,the image quality was improved by designing a special visual system that employed an endoscope and a *** also includes pixel brightness-based and gradient-based algorithms that can adaptively detect feature points at the boundary when the weld pool geometry *** algorithm can also be applied to materials with different solidification *** was performed to measure the real weld pool length in world coordinates,and outlier rejection was performed to increase the accuracy of the ***,tests were carried out on the intersection component,and the results showed that the proposed method performed well in tracking the changing weld pool length and was applicable to the real-time monitoring of different types of materials.

关键词： Plasma arc additive manufacturing(PAAM) Weld pool geometry Gradient analysis Real-time detection

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A New Solution to Intrusion Detection Systems Based on Improved Federated-Learning Chain

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Computers, Materials & Continua 2024年第6期79卷 4491-4512页

作者： Chunhui Li hua Jiang School of Computer and Electronic Information Guangxi UniversityNanning530000China Cyber Security and Information Center Guangxi UniversityNanning530000China

In the context of enterprise systems,intrusion detection(ID)emerges as a critical element driving the digital transformation of *** systems spanning various sectors of enterprises geographically dispersed,the necessity for seamless information exchange has surged *** existing cross-domain solutions are challenged by such issues as insufficient security,high communication overhead,and a lack of effective update mechanisms,rendering them less feasible for prolonged application on resource-limited *** study proposes a new cross-domain collaboration scheme based on federated chains to streamline the server-side *** this framework,individual nodes solely engage in training local data and subsequently amalgamate the final model employing a federated learning algorithm to uphold enterprise systems with efficiency and *** curtail the resource utilization of blockchains and deter malicious nodes,a node administration module predicated on the workload paradigm is introduced,enabling the release of surplus resources in response to variations in a node’s contribution *** encountering an intrusion,the system triggers an alert and logs the characteristics of the breach,facilitating a comprehensive global update across all nodes for collective *** results across multiple scenarios have verified the security and effectiveness of the proposed solution,with no loss of its recognition accuracy.

关键词： Cross-domain collaboration blockchain federated learning contribution value node management release slack resources

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