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喉癌N6-甲基腺嘌呤甲基化修饰差异基因的初步筛查与验证

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中国耳鼻咽喉头颈外科 2022年第4期29卷 239-242页

作者：赵学辉李秋影阚轩王婧婷孙亚男哈尔滨医科大学附属第二医院耳鼻咽喉头颈外科黑龙江哈尔滨150080

目的分析喉癌中N^(6)-甲基腺嘌呤(N^(6)-methyladenosine,m^(6)A)甲基化修饰差异基因表达水平,探讨其在喉癌进展中的作用。方法采用m^(6)A-mRNA表观转录组芯片检测3对喉癌组织及相应癌旁组织中m^(6)A甲基化修饰差异表达基因,并选择显... 详细信息

目的分析喉癌中N^(6)-甲基腺嘌呤(N^(6)-methyladenosine,m^(6)A)甲基化修饰差异基因表达水平,探讨其在喉癌进展中的作用。方法采用m^(6)A-mRNA表观转录组芯片检测3对喉癌组织及相应癌旁组织中m^(6)A甲基化修饰差异表达基因,并选择显著差异表达的含有6个跨膜BAX抑制子基序(transmembrane BAX inhibitor motif containing 6,TMBIM6)基因行逆转录实时定量PCR(RT-qPCR)、甲基化RNA免疫沉淀定量PCR(methylated RNA immunoprecipitation-qPCR,MeRIP-qPCR)及免疫组织化验证,最后检测其甲基化位点。结果在差异表达基因和m^(6)A甲基化基因的联合分析中,共获得135个高甲基化高表达基因,2958个高甲基化低表达基因,129个低甲基化高表达基因,682个低甲基化低表达基因。对显著高表达的TMBIM6基因行RT-qPCR、MeRIP-qPCR和免疫组化验证结果与基因芯片一致,鉴定出TMBIM6基因甲基化位点位于3’非编码区的2222碱基位。结论 TMBIM6基因通过m^(6)A修饰机制在喉癌中发挥癌基因的作用,可望成为喉癌的分子标记物及潜在的治疗靶点。

关键词：喉肿瘤逆转录聚合酶链反应免疫组织化学 m^(6)A甲基化修饰差异基因

Dithienopyrrole Based Small Molecule with Low Band Gap for Organic Solar Cells

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Chinese Journal of Chemistry 2015年第8期33卷 852-858页

作者： Miaomiao Li Wang Ni Huanran Feng Bin kan Xiangjian Wan Yamin Zhang xuan Yang Yongsheng Chen Key Laboratory for Functional Polymer Materials Centre for Nanoscale Science and TechnologyInstitute of Polymer Chemistry Collaborative Innovation of Chemical Science and Engineering ( Tianjin) College of Chemistry Nankai University Tianjin 300071 China

A low band gap acceptor-donor-acceptor （A-D-A） small molecule donor material, named DR3TDTN, has been designed and synthesized for solution-processed organic solar cells. DR3TDTN shows narrow optical band gap with value of 1.49 eV and broad absorption spectrum from 300 to 820 rim. The HOMO and LUMO energy levels of DR3TDTN are -4.74 and -3.26 eV, respectively. The optimized photovoltaic device based on DR3TDTN：PC71BM blend film shows a power conversion efficiency of 3.03% with an open-circuit voltage of 0.67 V, a short-circuit current of 8.22 mA·cm^-2 and fill factor of 0.55.

关键词： organic solar cells low band gap small molecule HOMO energy level

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基于物联网电器参数监测装置的研究

智能计算机与应用 2018年第6期8卷 111-112,115页

作者：胡林峰上官天钧倪志峰阚旋郑暮寅丁苑沁上海工程技术大学电子电气工程学院上海201620

采用微控制器STM32F407与高性能电能计量芯片90E36A结合的方案,设计了一款三相电器参数监测的装置。以单片机控制整个电路,硬件外设采用互感器调节整理电路,以计量芯片90E36A为监测核心的电路,ESP8266为无线传输电路的核心,服务器为数... 详细信息

采用微控制器STM32F407与高性能电能计量芯片90E36A结合的方案,设计了一款三相电器参数监测的装置。以单片机控制整个电路,硬件外设采用互感器调节整理电路,以计量芯片90E36A为监测核心的电路,ESP8266为无线传输电路的核心,服务器为数据储存的载体。软件方面,单片机芯片采用C语言编程,服务器采用腾讯云服务器。该装置能够实现对电器的电参数和工作状态的实时采集、显示、存储、远程通信和监测分析功能。

关键词： STM32F407 90E36A 远程监测物联网

Investigation of Edge Localized Fluctuation in KT-5C Tokamak

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Chinese Physics Letters 1996年第9期13卷 685-688页

作者： ZHAI kan WEN Yi-zhi YU Chang-xuan LIU Wan-dong WANG Chao ZHUANG Ge XU Zhi-zhan Department of Modern Physics University of Science and Technology of ChinaHefei 230027 Shanghai Institute of Optics and Fine Mechanics Chinese Academy of SciencesShanghai 201800

It is found on KT-5C tokamak that an edge fluctuation mode can be excited by using a pair of floating Langmuir *** mode is propagating along the magnetic field with its amplitude decreasing drastically when it is off the magnetic field *** feedback experiments show that the broadband edge turbulence can be reduced or enhanced depending upon the phase shift of the feedback network.

关键词： tokamak mode turbulence

Enhanced charge separation by continuous homojunction with spatially separated redox sites for hydrogen evolution

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Nano Research 2023年第10期16卷 12323-12330页

作者： Mingyuan Yu Tongyu Wang Chengxi Huang Fang Wu xuan Liu Hailing Huo Hanwen Jian Zikun Liang Jingjing Ma Erjun kan Ang Li MIIT Key Laboratory of Semiconductor Microstructure and Quantum Sensing School of ScienceNanjing University of Science and TechnologyNanjing 210094China College of Information Science and Technology Nanjing Forestry UniversityNanjing 210037China State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering Ningxia UniversityYinchuan 750021China

Photocatalytic hydrogen generation represents a promising strategy for the establishment of a sustainable and environmentally friendly energy ***,the current solar-to-hydrogen conversion efficiency is not yet sufficient for practical hydrogen production,highlighting the need for further research and ***,we report the synthesis of a Sn-doped TiO_(2)continuous homojunction hollow sphere,achieved through controlled calcination *** incorporation of a gradient doping profile has been demonstrated to generate a gradient in the band edge energy,facilitating carrier orientation ***,the hollow sphere’s outer and inner sides provide spatially separated reaction sites allowing for the separate acceptance of holes and electrons,which enables the rapid utilization of carriers after *** a result,the hollow sphere TiO_(2)with gradient Sn doping exhibits a significantly increased hydrogen production rate of 20.1 mmol·g^(−1)·h^(−1).This study offers a compelling and effective approach to the designing and fabricating highly efficient nanostructured photocatalysts for solar energy conversion applications.

关键词： continuous homojunction spatially separated reaction sites charge separation hydrogen evolution

地黄通过雌激素受体干预脂多糖诱导的脓毒症急性肾损伤

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中国新药与临床杂志 2023年第4期42卷 240-248页

作者：张贝贝曾梦楠阚玉璇张钦钦贾菊芳刘萌郭彭莉王茹冯卫生郑晓珂河南中医药大学药学院河南郑州450046 河南省中药开发工程技术研究中心河南郑州450046

目的研究鲜地黄和生地黄对脓毒症急性肾损伤的干预作用及与雌激素之间的关系。方法 120只雄性BALB/c小鼠随机分为正常对照组,拮抗剂对照组,模型组、阳性药组、鲜地黄组、生地黄组以及相对应的+拮抗剂组,每组12只。小鼠以腹腔注射脂多糖... 详细信息

目的研究鲜地黄和生地黄对脓毒症急性肾损伤的干预作用及与雌激素之间的关系。方法 120只雄性BALB/c小鼠随机分为正常对照组,拮抗剂对照组,模型组、阳性药组、鲜地黄组、生地黄组以及相对应的+拮抗剂组,每组12只。小鼠以腹腔注射脂多糖(LPS)5 mg·kg^(-1)建立脓毒症急性肾损伤模型,阳性药组、鲜地黄组、生地黄组分别灌胃黄芪注射液4 g·kg^(-1)、鲜地黄水提物溶液10 g·kg^(-1)、生地黄水提物溶液5 g·kg^(-1),其余组灌胃等量蒸馏水,每日1次,连续6 d。+拮抗剂组小鼠在每日给药前0.5 h腹腔注射雌激素受体特异性拮抗剂(ICI 182780)0.5 mg·kg^(-1),其余各组注射等量生理盐水。观察各组肾组织病理变化,检测血清中尿素氮(BUN)、肌酐(Scr)和细胞因子白细胞介素(IL)-6、肿瘤坏死因子(TNF)-α、IL-17A、干扰素(IFN)-γ水平,检测原代肾细胞凋亡和活性氧(ROS)水平,检测肾脏中Toll样受体4(TLR4)、caspase-11、NOD样受体热蛋白结构域相关蛋白3(NLRP3)和IL-1β mRNA水平及TLR4/核因子(NF)-κB-信号转导与转录激活因子1 (STAT1)和caspase-11/NLRP3信号通路相关蛋白的表达。结果与正常对照组相比,模型组小鼠出现肾小球萎缩、肾小管扩张、上皮细胞脱落等肾脏损伤,血清中Scr、BUN水平和细胞因子IL-6、TNF-α、IL-17A、IFN-γ水平显著升高,原代肾细胞ROS和凋亡水平升高,肾脏TLR4、caspase-11、NLRP3和IL-1β mRNA水平升高,相关通路蛋白TLR4、IFN-β、NF-κB p-p65、p-STAT1、caspase-11、NLRP3和IL-1β显著升高,IL-1受体拮抗剂表达水平显著降低(P <0.01)。鲜地黄和生地黄可有效抑制LPS诱导的肾脏损伤,显著降低BUN、Scr、细胞因子及原代肾细胞ROS和细胞凋亡水平,有效调节TLR4/NF-κB-STAT1和caspase-11/NLRP3信号通路的相关基因和蛋白表达水平(P <0.05),上述作用被雌激素受体拮抗剂减弱(P <0.05)。结论鲜地黄和生地黄可能通过雌激素受体调节TLR4/NF-κB-STAT1和caspase-11/NLRP3通路减轻LPS诱导的脓毒症急性肾损伤。

关键词：地黄脂多糖类急性肾损伤受体,雌激素

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Reconcile the contradictory wettability requirements for the reduction and oxidation half-reactions in overall CO_(2) photoreduction via alternately hydrophobic surfaces

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Journal of Energy Chemistry 2024年第6期93卷 202-212,I0006页

作者： Hailing Huo Ting Hu Chengxi Huang Fang Wu Tongyu Wang xuan Liu Liang Zhang Qiang Ju Zhiqing Zhong Hongbin Xing Erjun kan Ang Li MIIT Key Laboratory of Semiconductor Microstructure and Quantum Sensing Nanjing University of Science and TechnologyNanjing 210094JiangsuChina College of Information Science and Technology Nanjing Forestry UniversityNanjing 210037JiangsuChina

The overall photocatalytic CO_(2) reduction reaction(OPCRR)that can directly convert CO_(2) and H_(2)O into fuels represents a promising renewable energy conversion *** a typical redox reaction,the OPCRR involves two half-reactions:the CO_(2) reduction half-reaction(CRHR)and the water oxidation half-reaction(WOHR).Generally,both half-reactions can be promoted by adjusting the wettability of ***,there is a contradiction in wettability requirements for the two ***,CRHR prefers a hydrophobic surface that can accumulate more CO_(2) molecules on the active sites,ensuring the appropriate ratio of gas-phase(CO_(2))to liquid-phase(H_(2)O)***,the WOHR prefers a hydrophilic surface that can promote the departure of the gaseous product(O_(2))from the catalyst surface,preventing isolation between active sites and the reactant(H_(2)O).Here,we successfully reconciled the contradictory wettability requirements for the CRHR and WOHR by creating an alternately hydrophobic *** was achieved through a selectively hydrophobic modification method and a charge-transfer-control ***,the collaboratively promoted CRHR and WOHR led to a significantly enhanced OPCRR with a solar-to-fuel conversion efficiency of 0.186%.Notably,in ethanol production,the catalyst exhibited a 10.64-fold increase in generation rate(271.44μmol g^(-1)h~(-1))and a 4-fold increase in selectivity(55.77%)compared to the benchmark *** innovative approach holds great potential for application in universal overall reactions involving gas participation.

关键词： Hydrophobic Hydrophilic Gas transport Overall CO_(2)photoreduction Z-scheme

Phosphorylation of PHF2 by AMPK releases the repressive H3K9me2 and inhibits cancer metastasis

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Signal Transduction and Targeted Therapy 2023年第4期8卷 1857-1873页

作者： Ying Dong Hao Hu xuan Zhang Yunkai Zhang Xin Sun Hanlin Wang Weijuan kan Min-jia Tan Hong Shi Yi Zang Jia Li State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China University of Chinese Academy of Sciences Beijing 100049China School of Pharmacy Henan UniversityKaifeng 475004China Department of Pharmacology Fudan UniversityShanghai 201203China Department of Anesthesiology Shanghai Pulmonary HospitalSchool of MedicineTongji UniversityShanghai 200433China Lingang laboratory Shanghai 201203China School of Pharmaceutical Science and Technology Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China Shandong Laboratory of Yantai Drug Discovery Bohai Rim Advanced Research Institute for Drug DiscoveryYantaiShandong 264117China Open Studio for Druggability Research of Marine Natural Products Pilot National Laboratory for Marine Science and Technology(Qingdao)1 Wenhai RoadAoshanweiJimoQingdao 266237China

Epithelial to mesenchymal transition (EMT) plays a crucial role in cancer metastasis, accompanied with vast epigenetic ***-activated protein kinase (AMPK), a cellular energy sensor, plays regulatory roles in multiple biological processes. Although afew studies have shed light on AMPK regulating cancer metastasis, the inside epigenetic mechanisms remain unknown. Herein weshow that AMPK activation by metformin relieves the repressive H3K9me2-mediated silencing of epithelial genes (e.g., CDH1)during EMT processes and inhibits lung cancer metastasis. PHF2, a H3K9me2 demethylase, was identified to interact with AMPKα*** deletion of PHF2 aggravates lung cancer metastasis and abolishes the H3K9me2 downregulation and anti-metastasis effectof metformin. Mechanistically, AMPK phosphorylates PHF2 at S655 site, enhancing PHF2 demethylation activity and triggering thetranscription of CDH1. Furthermore, the PHF2-S655E mutant that mimics AMPK-mediated phosphorylation status further reducesH3K9me2 and suppresses lung cancer metastasis, while PHF2-S655A mutant presents opposite phenotype and reverses the antimetastasiseffect of metformin. PHF2-S655 phosphorylation strikingly reduces in lung cancer patients and the higherphosphorylation level predicts better survival. Altogether, we reveal the mechanism of AMPK inhibiting lung cancer metastasis viaPHF2 mediated H3K9me2 demethylation, thereby promoting the clinical application of metformin and highlighting PHF2 as thepotential epigenetic target in cancer metastasis.

关键词： metastasis lung cancer

High-density triple-phase contact points for enhanced photocatalytic CO_(2) reduction to methanol

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Chinese Chemical Letters 2024年第2期35卷 252-256页

作者： Hanwen Jian Kaiming Deng Tongyu Wang Chengxi Huang Fang Wu Hailing Huo Bo Ouyang xuan Liu Jingjing Ma Erjun kan Ang Li MIIT Key Laboratory of Semiconductor Microstructure and Quantum Sensing School of ScienceNanjing University of Science and TechnologyNanjing 210094China College of Information Science and Technology Nanjing Forestry UniversityNanjing 210037China State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering Ningxia UniversityYinchuan 750021China

The efficiency of photocatalytic CO_(2) reduction reaction(PCRR)is restricted by the low solubility and mobility of CO_(2) in water,poor CO_(2) adsorption capacity of catalyst,and competition with hydrogen evolution reaction(HER).Recently,hydrophobic modification of the catalyst surface has been proposed as a potential solution to induce the formation of triple-phase contact points(TPCPs)of CO_(2)(gas phase),H_(2) O(liquid phase),and catalysts(solid phase)near the surface of the catalyst,enabling direct delivery of highly concentrated CO_(2) molecules to the active reaction sites,resulting in higher CO_(2) and lower H+surface *** TPCPs thus act as the ideal reaction points with enhanced PCRR and suppressed ***,the initial synthesis of triple-phase photocatalysts tends to possess a lower bulk density of TPCPs due to the simple structure leading to limited active points and CO_(2) adsorption ***,based on constructing a hydrophobic hierarchical porous TiO_(2)(o-HPT)with interconnected macropores and mesopores structure,we have significantly increased the density of TPCPs in a unit volume of the *** with hydrophobic macroporous TiO_(2)(o-MacPT)or mesoporous TiO_(2)(o-MesPT),the o-HPT with increased TPCP density leads to enhanced photoactivity,enabling a high methanol production rate with 1111.5μmol g^(−1) h^(−1) from *** results emphasize the significance of high-density TPCPs design and propose a potential path for developing efficient PCRR systems.

关键词： Photocatalysis CO_(2)reduction reaction Photocatalyst Triple-phase contact points Methanol