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Phytochemical Determination and Antibacterial Activity of Punica granatum Peel Extracts against Plant Pathogenic Bacteria

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American Journal of Plant Sciences 2016年第1期7卷 159-166页

作者： Ayad Ismael Khaleel kamaruzaman sijam Tavga Sulaiman Rashid Khairulmazmi Bin Ahmad Plant Protection Department Faculty of Agriculture Universiti Putra Malaysia Serdang Malaysia

Plant pathogenic bacteria are recognized to be harmful microbes able to decrease the quantity and quality of crop production in the world. Punica granatum peel was screened for its potential use as biological control agent for plant pathogenic bacteria. P. granatum peel was successfully extract using n-hexane, methanol and ethyl acetate by maceration. The highest yield obtained by ethyl acetate showed that ethyl acetate extracted more compounds that readily soluble to methanol and n-hexane. For in-vitro antibacterial activity, three different species of plant pathogenic bacteria were used namely Erwinia carotovorum subsp. Carotovorum, Ralstonia solanacearum, and Xanthomonas gardneri. For all crude extracts, four different concentrations 25, 50, 100 and 200 mg/ml were used in cup-plate agar diffusion method. Streptomycin sulfate at concentration 30 μg/ml was used as positive control while each respective solvent used for peel extraction was used as negative control. The results obtained from in vitro studies showed only ethyl acetate extract possessed antibacterial activity tested on the plant pathogenic bacteria. Methanol and n-hexane did not show any antibacterial activity against plant pathogenic bacteria selected where no inhibition zones were recorded. R. solanacearum recorded the highest diameter of inhibition zones for all range of concentrations introduced followed by E. carotovorum subsp. Carotovorum and X. gardneri. For the minimum inhbitory concentration (MIC) and minimum bactericidal concentration (MBC), only the ethyl acetate extract was subjected to the assay as only ethyl acetate extract exhibited antibacterial activity. The minimum concentration of ethyl acetate extract that was able to inhibit plant pathogenic bacteria was recorded at a concentration of 3.12 mg/ml which inhibited R. solancearum and E. carotovorum subsp. Carotovorum, followed by X. gardneri at concentration 6.25 mg/ml. For the minimum bactericidal concentration (MBC), the results show

关键词： Punica granatum Plant Extraction Pathogenic Bacteria Phytochemical Screening

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Gc-Ms Analysis and Antibacterial Activity of Mangosteen Leaf Extracts against Plant Pathogenic Bacteria

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American Journal of Plant Sciences 2016年第7期7卷 1013-1020页

作者： Qamar Mohammed Naji Alsultan kamaruzaman sijam Tavga Sulaiman Rashid Khairulmazmi Bin Ahmad Department of Plant Protection Faculty of Agriculture University Putra Malaysia UPM Serdang Malaysia

The potential of Garcinia mangostana as a biological control agent against plant pathogenic bacteria which decrease the quality and volume of crop production worldwide was assessed. Mangosteen leaves were extracted by maceration using chloroform, n-hexane, and methanol. For the in vitro antibacterial activity, two dissimilar species of plant pathogenic bacteria: Pseudomonas syringe pv. tomato and Xanthomonas oryzae pv. oryzae were acquired. Four different concentrations, 12.5, 25, 50, and 100 mg/ml were obtained through the cup-plate agar diffusion technique. Streptomycin sulphate at 30 μg/ml concentration was set as the positive control, whereas every respective solvent used in the leaf extraction was set as the negative control. The results have shown that, only methanol extract demonstrated antibacterial activity when tested on the plant pathogenic bacteria. The highest diameter of inhibition zones was observed in X. oryzae pv. oryzae, at all range of concentrations, followed by P. syringae pv. tomato. The least methanol extract concentration utilised in determination of minimum inhibitory concentration (MIC) assay was at 1.562 mg/ml, inhibiting X. oryzae pv. oryzae, followed by P. syringe pv. tomato at a concentration 3.125 mg/ml. Antibacterial impacts of the most effectual extract of mangosteen crude were supported by the existence of chemical components identified by GC-MS. Cycloartenol, Caryophyllene, Docosane, Phenol, 4,4-Methylenebis (2,6-di-tert-butylphenol) and Chromium were noted as key compounds in the mangosteen leaf extract, which were perhaps causing the antibacterial activity.

关键词： Pseudomonas syringae Xanthomonas oryzae Garcinia mangostana and Methanol Extract

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Pomelo,a resistance variety to Citrus tristeza virus in peninsular Malaysia

Pomelo,a resistance variety to Citrus tristeza virus in peni...

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International Conference on Biotechnology and Food Science（ICBFS 2011）

作者： Kavous Ayazpour kamaruzaman sijam Ganesan Vadamalai Hawa Jaafar Plant Protection Department University Putra Malaysia Serdang Malaysia

Citrus tristeza virus（CTV）is the most important viral disease of Citrus and is distributed *** of ELISA and PCR methods showed that all Citrus varieties including Fortunella sp.,Citrofortunella microcarpa and Citromelo in major citrus growing areas of Malaysia were infected with CTV in a high *** most areas,pomelo however was free of infection,but in Cameron Highlands,we found some strains of CTV that were severe to Citromelo and *** studies revealed that these strains were familiar to CTV isolates from China and Japan and were very differ from CTV isolates of USA and New Zealand.

关键词： Citrus tristeza virus Phylogeny analysis Strain CP gene Malaysia

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Pathogenicity Assay and Molecular Identification of Fungi and Bacteria Associated with Diseases of Tomato in Malaysia

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American Journal of Plant Sciences 2016年第6期7卷 949-957页

作者： Tavga Sulaiman Rashid kamaruzaman sijam Hayman Kakakhan Awla Halimi Mohd Saud Jugah Kadir Department of Plant Protection Faculty of Agriculture University Putra Malaysia Serdang Malaysia Department of Plant Protection Faculty of Agriculture University of Salahaddin Erbil Iraq

This study was conducted in order to determine the fungi and bacteria associated with tomato plants at Cameron Highlands Malaysia. The fungi which have been isolated and detected from tomato plants were: Fusarium oxysporum, F. solani, F. acuminatum, Rhizoctonia solani, Colletotrichum boninense, C. acutatum and Phoma destructiva. The bacteria which have been isolated and detected from tomato plants were: Ralstonia solanacearum, Xanthomonas vesicatoria, X. gardneri and Pseudomonas syringae. While the most pathogenic fungi were C. boninense, P. destructive and F. oxysporum with the disease incidence (89.6%, 86.6%, 85.6%) respectively, the most pathogenic bacteria were X. vesicatoria and R. solanacearum with the disease incidence (96.6% and 87.6%) respectively.

关键词： Lycopersicon esculentum Pathogen Isolation and Molecular Identification

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Biological Control of <i>Pyricularia oryzae</i>Using Antifungal Compounds Produced by <i>Aspergillus niger</i>

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American Journal of Plant Sciences 2017年第10期8卷 2445-2460页

作者： Ali Abdulameer Idan kamaruzaman sijam Jugah Kadir Tavga Sulaiman Rashid Hayman Kakakhan Awla Wael Alsultan Plant Protection Department Faculty of Agriculture Universiti Putra Malaysia Serdang Malaysia Department of Plant Protection Faculty of Agriculture University of Kufa Najaf Iraq Department of Plant Protection Faculty of Agriculture University of Salahaddin Erbil Iraq Department of Plant Protection Khabat Institute Erbil Polytechnic University Erbil Iraq

Aspergillus spp. has been widely found as useful microorganism in biotechnology. They have a high ability in the production of secondary metabolites. Therefore, isolates of Aspergillus were isolated from healthy rice field located in Selangor State/Malaysia. The obtained strain (UPMZ01) was conducted against Pyricularia oryzea by applying dual culture and culture filtrate technique. The antagonism of strain UPMZ01 in the dual culture was 81.326% inhibition percentage against P. oryzae given the optimum inhibitory percentage 100% at all concentration of secondary metabolites aged 14 days. The isolate (UPMZ01) was identified as Aspergillus niger with accession number (KY698415). The environment factors such as pH and temperature influencing on production of secondary metabolites. The results were shown that pH at level 5.0 and temperature between 21℃?to 29℃?is the optimum condition for A. niger to produce efficient antifungal metabolites which given 100% PIGR against blast pathogen. The secondary metabolites compounds were identified by Gas Chromatography-Mass Spectrometry (GC-MS). Fifteen compounds were recognized as major compounds which may have the possibility of possessing antifungal characteristics. Most of identified compounds are Oleic Acid, n-Hexadecanoic acid, Hexose, Glycerol, Stearic acid, Tetradecanoic acid, Dodecanoic acid and 5-Hydrxoymethylfurfural.

关键词： Blast Disease Aspergillus sp. Pyricularia oryzae Antifungal Compounds GC-MS

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