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益气活血方对球囊损伤大鼠颈总动脉后Cyclinb_1异常表达的影响

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四川中医 2010年第12期28卷 14-15页

作者：刘彬符逢春徐慧刘起胜瞿晓灵湖南中医药高等专科学校湖南株洲412000

目的:观察损伤血管细胞周期蛋白Β1(Cyclinb1)的表达情况,探讨益气活血方对球囊损伤大鼠颈总动脉内膜平滑肌细胞(VSMCs)增殖的干预机制。方法:随机将90只雄性SD大鼠分为模型组,阿司匹林组,益气活血方大、中、小剂量组。球囊损伤大鼠颈... 详细信息

目的:观察损伤血管细胞周期蛋白Β1(Cyclinb1)的表达情况,探讨益气活血方对球囊损伤大鼠颈总动脉内膜平滑肌细胞(VSMCs)增殖的干预机制。方法:随机将90只雄性SD大鼠分为模型组,阿司匹林组,益气活血方大、中、小剂量组。球囊损伤大鼠颈总动脉前给予高脂饲料喂养30天,造成高脂血症;然后经右股动脉穿刺球囊扩张损伤大鼠左颈总动脉,造成颈总动脉管腔的狭窄。手术前1天开始给药,每组动物于术后(5d、14d、28d)处死,取损伤颈总动脉,常规固定、包埋、切片,分别做HE染色和免疫组织化学SAbC法检测Cyclinb1的表达。结果:模型缓慢Cyclinb1表达明显增多,用益气活血方干预后,14d和28dCyclinb1表达量与模型组比较差异有显著性(Pb1的表达,抑制血管内膜VSMCs的细胞分裂周期进程有关。

关键词：经皮腔内冠状动脉成形术益气活血球囊损伤大鼠细胞周期蛋白b1

A Regional Climate Study of Heat Waves over the Iberian Peninsula

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Atmospheric and Climate Sciences 2014年第5期4卷 841-853页

作者： Hari Prasad Dasari Isaac Pozo Francisco Ferri-Yánez Miguel b. Araújo Centro de Geofisica de Evora University of EvoraEvoraPortugal Museo Nacional de Ciencias Naturales CSICMadridSpain Centro de biodiversidade e Recursos Genéticos(InbIO-CIbIO) University of EvoraEvoraPortugal Imperial College London Silwood Park CampusBerkshireUK

We present a regional climate simulation for the Iberian Peninsula for a 60-year period (1950-2009) using the WRF-ARW model with a focus on the simulation of summer maximum temperatures and associated extreme heat waves. The WRF model was designed at a 5 km horizontal resolution on a 5-month (May-September) seasonal scale, for every year, during the study period with initial/boundary conditions derived from NCEP 2.5 degree reanalysis. The comparison of simulated mean summer seasonal maximums and mean maximums of June, July, and August months with the corresponding E-ObS data sets indicates that the model is able to characterize the spatial variation of magnitudes of temperature change over the Iberian Peninsula. The mean extreme heat wave conditions during the climate period 1950-2009 are well simulated and match the observations well. The regional scale simulations clearly show the propagation of intense heat waves from the south west to north east of Iberia. The WRF-ARW model also simulated well the general trend of increase in heat waves over most parts of the Iberian Peninsula during the study period 1950-2009. The characteristics of the most severe heat waves years 2003 and 2006 are also well simulated by the model.

关键词： Downscaling Heat Waves Iberian Peninsula Regional Climate Modelling

口服双磷酸盐对囊性纤维化和低骨矿物质密度患者的影响

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世界核心医学期刊文摘（儿科学分册） 2005年第5期1卷 30-31页

作者： Ringuier b. Leboucher b. Leblanc M. 郭战宏

Disturbances in bone mineralization are frequent in cystic fibrosis but few st udies have focused on the use of biphosphonates in this indication, and none on the use of oral etidronate. We report our experience using this latter treatment . Methods. -The study was retrospective and included five children and three ad ults with cystic fibrosis (six males and two females) aged seven to 30:years wit h Z-scores for lumbar bone density lower than-2:SD after one year of calcium ( 1:g/day) and vitamin D (900:ui/day and 300:000:ui/6:months) supplementation. All were treated during one year with etidronate: four courses of 15:days (one cour se per trimester) with doses ranging from 4 to 8:mg/kg per day. Calcium and vita min D supplementation was continued between the etidronate treatment course. Tot al body and lumbar bone mineral density (bMD) were measured three times: at the beginning and the end of the year of calcium and vitamin D supplementation and at the end of the year of supplementation plus the four courses of etidronate treatment. Resu lts. -The increase in bMD in absolute value (g/cm2) and in Z-score was signifi cantly higher (P bined supplementation and etidron ate treatment (total body g/cm2: 3 ±1%, Z-score: 2 ±1%and lumbar spine g/cm 2: 6 ±5%, Z-score: 3 ±4%) than after supplementation alone (total body g/cm 2:-1 ±3%, Z-score:-4 ±3%and lumbar spine g/cm2:-1 ±3%, Z-score:-4 ± 4%). Supplementation alone improved the total bMD in only one patient and the l umbar bMD in three, whereas after etidronate treatment the total and lumbar bMD were improved in the eight patients. None of the patients presented with side ef fects that could be attributed to the treatment. Conclusion. -Oral etidronate t reatment is well-tolerated and capable of improving bone mineralization in pati ents with cystic fibrosis. Further work will be necessary to determine the optim al dosage and the optimal frequency for the treatment series.

关键词：骨矿物质密度囊性纤维化腰椎骨密度双磷酸盐囊肿性纤维化最佳剂量给药频率骨矿化

Species of Lasiodiplodia associated with mango in brazil

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Fungal Diversity 2013年第4期61卷 181-193页

作者： Marília W.Marques Nelson b.Lima Marcos Antônio de Morais Jr Maria Angélica G.barbosa breno O.Souza Sami J.Michereff Alan J.L.Phillips Marcos P.S.Câmara Departamento de Agronomia Universidade Federal Rural de Pernambuco52171-900 RecifeBrazil Departamento de Genética Universidade Federal de Pernambuco50732-970 RecifeBrazil Embrapa Semi-Árido 56302-970 PetrolinaBrazil Centro de Recursos Microbiológicos Departamento de Ciências da VidaFaculdade de Ciências e TecnologiaUniversidade Nova de Lisboa2829-516 CaparicaPortugal

Mango(Mangifera indica)is a major tropical fruit species cultivated in *** objective of this study was to identify species of Lasiodiplodia associated with dieback and stem-end rot of mango in the semi-arid region of Northeastern brazil,and compare the species in relation to mycelial growth,pathogenicity and virulence.A total of 120 isolates of Lasiodiplodia were used and identifications were made using a combination of morphology and phylogenetic analysis based on partial translation elongation factor 1-αsequence(EF1-α)and internal transcribed spacers(ITS).The following species were identified:Lasiodiplodia crassispora,***,***,***,***,*** and Lasiodiplodia sp..Lasiodiplodia theobromae was the most frequently isolated species,which represented 41%of all the *** this species had been previously reported on mango in brazil,while the other species represent the first report associated with mango tree diseases in this *** crassispora is reported for the first time associated with mango diseases *** were significant differences in mycelial growth rates among the Lasiodiplodia species and also in the optimum temperature for *** species of Lasiodiplodia were pathogenic on mango *** were significant differences in virulence among the species,wherein *** and Lasiodiplodia *** the most virulent,while the least virulent were ***,***,*** and ***.

关键词： botryosphaeriaceae Mangifera indica ITS EF1-α Phylogeny Virulence

Species of Lasiodiplodia associated with papaya stem-end rot in brazil

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Fungal Diversity 2014年第4期67卷 127-141页

作者： Mariote S.b.Netto Iraíldes P.Assuncao Gaus S.A.Lima Marília W.Marques Waléria G.Lima Jean H.A.Monteiro Valdir de Queiroz balbino Sami J.Michereff Alan J.L.Phillips Marcos P.S.Camara Departamento de Agronomia Universidade Federal Rural de Pernambuco52171-900 RecifePernambucoBrazil Centro de Ciencias Agrárias Universidade Federal de Alagoas57100-000 Rio LargoAlagoasBrazil Departamento de Genética Universidade Federal de Pernambuco50732-970 RecifeBrazil Centro de Recursos Microbiológicos Departamento de Ciencias da VidaFaculdade de Ciencias e TecnologiaUniversidade Nova de Lisboa2829-516 CaparicaPortugal

This study aims to identify and characterize species of Lasiodiplodia associated with stem-end rot of papaya in six different populations in the Northeast of *** identifications were made using a combination of morphology together with a phylogenetic analysis based on partial translation elongation factor 1-αsequence(EF-1α)and internal transcribed spacers(ITS).Five species of Lasiodiplodia were identified:Lasiodiplodia brasiliense ***.,***,*** ***.,*** and *** *** had previously been reported in papaya,while all the other species are reported for the first time in association with this host in brazil and *** theobromae was the most prevalent *** species of Lasiodiplodia were pathogenic on papaya fruit,with *** being the most virulent.

关键词： botryosphaeriaceae Carica papaya Fruit rot EF1-α ITS Phylogeny Virulence

清热活血汤直肠滴注治疗溃疡性结肠炎的实验研究

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中国肛肠病杂志 2018年第5期38卷 7-10页

作者：石强刘翠萍潘贺聂旋田昕杨增凤张扬司晶马丹丹齐齐哈尔市中医医院肛肠科黑龙江齐齐哈尔161000

为观察清热活血汤直肠滴注治疗溃疡性结肠炎(UC)模型小鼠的效果,以期为该药用于临床治疗UC提供理论依据,将40只bL/C雄性小鼠随机分为A、b、C、D4组,各10只。各组小鼠均应用葡聚糖硫酸钠(DSS)建立UC模型,造模成功后正常饲养5 d后,A组不... 详细信息

为观察清热活血汤直肠滴注治疗溃疡性结肠炎(UC)模型小鼠的效果,以期为该药用于临床治疗UC提供理论依据,将40只bL/C雄性小鼠随机分为A、b、C、D4组,各10只。各组小鼠均应用葡聚糖硫酸钠(DSS)建立UC模型,造模成功后正常饲养5 d后,A组不予治疗,b、C组分别予高(25 mL/kg)、中(20 mL/kg)剂量的清热活血汤直肠滴注,D组予美沙拉嗪水溶液(20 mg/kg)直肠滴注,连续14 d。观察各组小鼠一般状态、体质量变化、存活时间。实验结束后,解剖各组小鼠,观察结肠外观并做病理检查。结果显示,b、C、D组小鼠一般状况优于A组,肉眼观察和组织切片发现各组UC症状明显改善。b、C、D组小鼠生存情况优于A组,差异均有统计学意义,Pb、C组与D组比较,差异无统计学意义,P>0.05。结果表明,清热活血汤对UC小鼠有明显的治疗作用,与美沙拉嗪同效。

关键词：溃疡性结肠炎小鼠清热活血汤直肠滴注治疗

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不同胎龄新生儿脑室内出血发生率的调查

上海医学 1998年第4期21卷 193-195页

作者：陈惠金吴圣楣潘琢如徐承静上海市儿科医学研究所 200092 上海第二医科大学新华医院

目的 :为了解不同胎龄新生儿脑室内出血 ( IHV)自然发生率。方法 :于 1996年 7月至 9月三个月内 ,对所有出生于上海第二医科大学附属新华医院产科的 330例新生儿在生后一周内进行头颅 b超检查 ,以调查不同胎龄新生儿脑室内出血的发生率... 详细信息

目的 :为了解不同胎龄新生儿脑室内出血 ( IHV)自然发生率。方法 :于 1996年 7月至 9月三个月内 ,对所有出生于上海第二医科大学附属新华医院产科的 330例新生儿在生后一周内进行头颅 b超检查 ,以调查不同胎龄新生儿脑室内出血的发生率。结果 :330例新生儿的平均胎龄为 39.6± 1.9( 2 8～ 4 2 .6)周 ,出生体重为 3.36± 0 .4 9( 1.2 9～ 4 .84 ) kg。总的 IVH和严重 IVH的发生率分别为 15.8%( 52 / 330 )和 3% ( 10 / 330 )。其中早产儿分别为 76.5% ( 13/ 17)和 35.3% ( 6/ 17) ,足月儿分别为 12 .5%( 39/ 313)和 1.3% ( 4 / 313)。所有 IVH患儿均无明显的临床症状。资料显示 IVH主要发生在出生体重偏低和胎龄偏小的新生儿中。臀位分娩与 IVH的发生有统计学意义。妊高症的轻重程度似与 IVH有一定关联 ,Apgar评分亦有偏低趋势 ,但均无统计学意义。分娩期间常易发生胎儿全身血压的突然紊乱 ,或可解释正常的分娩过程也会引起 IVH的发生。结论 :本文进一步证实了所有新生儿在生后一周内均应进行头颅

关键词：新生儿脑室内出血发生率 b超

槐耳清膏对肺腺癌细胞增殖凋亡及相关基因表达的影响

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国际检验医学杂志 2015年第11期36卷 1483-1485页

作者：王卉吴唐维刘水逸李晓怡陈卫群卢宏达孔庆志卢忠心武汉市中心医院检验科湖北武汉430014 武汉市肿瘤研究所湖北武汉430014 武汉市中心医院中心实验室湖北武汉430014

目的探讨槐耳清膏抑制肺腺癌细胞增殖、促进细胞凋亡的抗肿瘤作用的分子机制。方法应用不同浓度槐耳清膏处理肺腺癌细胞,细胞增殖活性利用羧基荧光素二醋酸盐琥珀酰亚胺酯(CFSE)染色法检测,细胞凋亡应用Annexin V-FITC/PI双染流式细胞... 详细信息

目的探讨槐耳清膏抑制肺腺癌细胞增殖、促进细胞凋亡的抗肿瘤作用的分子机制。方法应用不同浓度槐耳清膏处理肺腺癌细胞,细胞增殖活性利用羧基荧光素二醋酸盐琥珀酰亚胺酯(CFSE)染色法检测,细胞凋亡应用Annexin V-FITC/PI双染流式细胞术检测,细胞中相关基因表达利用免疫技术检测。结果 CFSE染色结果发现槐耳能够明显抑制肺腺癌细胞的增殖,并具有浓度和时间依赖性;Annexin V-FITC/PI双染流式细胞术检测细胞凋亡结果发现槐耳能够促进肺腺癌细胞凋亡,具有浓度和时间依赖性;免疫蛋白印迹实验结果发现在肺腺癌细胞中,槐耳能够抑制相关基因EZH2、β-catenin和bcl-2的表达。结论在肺腺癌细胞中槐耳清膏抗肿瘤作用的分子机制可能是通过抑制EZH2/β-catenin信号通路,抑制肺腺癌细胞增殖、促进细胞凋亡。

关键词：槐耳清膏肺癌细胞增殖细胞凋亡分子机制

指标在流域管理规划和战略环境评价过程中的应用(下)

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环境影响评价 2019年第5期41卷 I0005-I0013页

作者： Rosa Santos Coelho Pedro S.Coelho Tomas b.Ramos Paula Antunes 吴成志(译) 张怡悦(译) 卿野(译) Faculdade de Ciencias e Tecnologia Departamento de Ciencias e Engenharia do AmbienteCenter for Environmental and Sustainability Research (CENSE)Universidade NOVA de LisboaCampus da CaparicaCaparicaPortugal Instituto Politecnico de Santarem Escola Superior AgrariaSantaremPortugal Faculdade de Ciencias e Tecnologia Departamento de Ciencias e Engenharia do AmbienteMarine and Environmental Sciences Centre (MARE)Universidade NOVA de LisboaCampus da CaparicaCaparicaPortugal 三捷环境工程咨询(杭州)有限公司

流域管理规划(RbMP)和战略环境影响评价(SEA)过程通常需要使用指标来评估规划方案的可持续性、与利益相关者进行沟通并对决策提供支持。这项研究的目的是为了支持主要过程和调查指标的使用效果,并分析整合后的情况。葡萄牙RbMP的内容分... 详细信息

流域管理规划(RbMP)和战略环境影响评价(SEA)过程通常需要使用指标来评估规划方案的可持续性、与利益相关者进行沟通并对决策提供支持。这项研究的目的是为了支持主要过程和调查指标的使用效果,并分析整合后的情况。葡萄牙RbMP的内容分析及相应的SEA报告采用了概念框架来支持其内容的定性分析。主要的发现是指标通常被用于SEA和规划报告中。被分析的文件中使用了大量的指标,其中大多数指标是定量的,并在组织、选择和验证时使用了差别很大的方法。通常这些过程都不需要公众参与。此研究给出了需要改进的方面,例如还需研究是否有需要增加利益相关者在指标发展过程中的参与度、标准化和交叉性指标使用时的相关性以及两个过程中都使用的关键性指标的确定。

关键词：河流流域管理规划环境影响评价指标概念框架内容分析