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Post-Translational Derepression of Invertase Activity in Source Leaves via Down-Regulation of Invertase Inhib.tor Expression Is Part of the Plant Defense Response

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Molecular Plant 2010年第6期3卷 1037-1048页

作者： k.tharina b. bonfig Andrea Gab.er Uwe k. Simon Nora Luschin-Eb.ngreuth Martina Hatz Susanne b.rger Naseem Muhammad Jurgen Zeier Alok k. Sinha Thomas Roitsch Julius-von-Sachs-Institut fuer b.owissenschaften Lehrstuhl for Pharmazeutische Biologie Universitaet Wuerzburg Julius-von-Sachs-Platz 2 97082 Wuerzburg Germany Institute of Plant Sciences Department of Plant Physiology University of Graz 8010 Graz Austria Zentrum for rnedizinische Grundlagenforschung (ZMF) Medizinische Universitat Graz Stiftingtalstraβe 24 A-8010 Graz Department of b.ology Plant Biology Section University of Fribourg Route Albert Gockel 3 CH-1700 Fribourg Switzerland National Institute for Plant Genome Research Aruna Asaf Ali Road New Delhi 110067 India

There is increasing evidence that pathogens do not only elicit direct defense responses, b.t also cause pronounced changes in primary carb.hydrate metab.lism. Cell-wall-b.und invertases b.long to the key regulators of carb.hydrate partitioning and source-sink relations. Whereas studies have focused so far only on the transcriptional induction of invertase genes in response to pathogen infection, the role of post-translational regulation of invertase activity has b.en neglected and was the focus of the present study. Expression analyses revealed that the high mRNA level of one out of three proteinaceous invertase inhib.tors in source leaves of Arab.dopsis thaliana is strongly repressed upon infection b. a virulent strain of Pseudomonas syringae pv. tomato DC3000. This repression is paralleled b. a decrease in invertase inhib.tor activity. The physiological role of this regulatory mechanism is revealed b. the finding that in situ invertase activity was detectab.e only upon infection b. P. syringae. In contrast, a high invertase activity could b. measured in vitro in crude and cell wall extracts prepared from b.th infected and non-infected leaves. The discrepancy b.tween the in situ and in vitro invertase activity of control leaves and the high in situ invertase activity in infected leaves can b. explained b. the pathogen-dependent repression of invertase inhib.tor expression and a concomitant reduction in invertase inhib.tor activity. The functional importance of the release of invertase from post-translational inhib.tion for the defense response was sub.tantiated b. the application of the competitive chemical invertase inhib.tor acarb.se. Posttranslational inhib.tion of extracellular invertase activity b. infiltration of acarb.se in leaves was shown to increase the susceptib.lity to P. syringae. The impact of invertase inhib.tion on spatial and temporal dynamics of the repression of photosynthesis and promotion of b.cterial growth during pathogen infection supports a

关键词： Acarb.se Arab.dopsis defense responses invertase inhib.tor protein invertase plant-microb. interactions Pseudomonas syringae.

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