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Split-HaloTag imaging assay for sophisticated microscopy of protein–protein interactions in planta

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Plant Communications 2021年第5期2卷 148-159页

作者： Rieke Minner-Meinen Jan-Niklas Weber Andreas Albrecht Rainer Matis Maria Behnecke Cindy Tietge Stefan Frank jutta schulze Henrik Buschmann Peter Jomo Walla Ralf-R.Mendel Robert Hänsch David Kaufholdt Institut für Pflanzenbiologie Technische Universität BraunschweigHumboldtstrasse 138106 BraunschweigGermany Institut für Physikalische und Theoretische Chemie Technische Universität BraunschweigHagenring 30.023c38106 BraunschweigGermany Botany Department Universität OsnabrückBarbara Strasse 1149076 OsnabrückGermany Center of Molecular Ecophysiology(CMEP) College of Resources and EnvironmentSouthwest UniversityTiansheng Road No.2Beibei District400715 ChongqingP.R.China

An ever-increasing number of intracellular multi-protein networks have been identified in plant ***-GFP-based protein–protein interaction assays combine the advantages of in vivo interaction studies in a native environment with additional visualization of protein complex *** of their simple protocols,they have become some of the most frequently used ***,standard fluorescent proteins present several drawbacks for sophisticated *** the HaloTag system,these drawbacks can be overcome,as this reporter forms covalent irreversible bonds with synthetic photostable fluorescent *** can be used in adjustable concentrations and are suitable for advanced microscopy ***,we have established the Split-HaloTag imaging assay in plants,which is based on the reconstitution of a functional HaloTag protein upon protein–protein interaction and the subsequent covalent binding of an added fluorescent *** suitability and robustness were demonstrated using a well-characterized interaction as an example of protein–protein interaction at cellular structures:the anchoring of the molybdenumcofactor biosynthesis complex to filamentous *** addition,a specific interactionwas visualized in a more distinctivemannerwith subdiffractional polarizationmicroscopy,Airyscan,and structured illumination microscopy to provide examples of sophisticated ***-GFPand Split-HaloTag can complement one another,as Split-HaloTag represents an alternative option and an addition to the large toolbox of in vivo ***,this promising new Split-HaloTag imaging assay provides a unique and sensitive approach formore detailed characterization of protein–protein interactions using specific microscopy techniques,such as 3D imaging,single-molecule tracking,and super-resolution microscopy.

关键词： advanced microscopy cytoskeleton photostable fluorescent dyes gateway cloning molybdenum cofactor biosynthesis complex protein-protein interaction Split-HaloTag imaging assay

Removal of endocrine disrupting chemicals from water through urethane functionalization of microfiltration membranes via electron beam irradiation

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Frontiers of Environmental Science & Engineering 2024年第4期18卷 61-74页

作者： Zahra Niavarani Daniel Breite Muhammad Yasir Vladimir Sedlarik Andrea Prager Nadja Schönherr Bernd Abel Roger Gläser Agnes schulze Leibniz Institute of Surface Engineering eV.(IOM) Permoserstrasse 1504318 LeipzigGermany Centre of Polymer Systems University InstituteTomas Bata University in ZlinTrida Tomase Bati 567876001 ZlinCzech Republic Institute of Chemical Technology Leipzig UniversityLinnestrasse 304103 LeipzigGermany

Polyethersulphone(PES)membranes modified with urethane functional groups were prepared through an interfacial reaction using electron beam *** removal of eight endocrine disrupting chemicals(EDCs)was studied using both pristine and functionalized PES *** prepared membranes underwent characterization using several techniques,including attenuated total reflectance-Fourier transform infrared(ATR-FTIR)spectroscopy,scanning electron microscopy,contact angle analysis,and measurements of pure water ***,dynamic adsorption experiments were conducted to evaluate the adsorption mechanism of the prepared membrane toward the eight *** urethane functionalized membranes were hydrophilic(52°contact angle)and maintained a high permeate flux(26000 L/h m^(2) bar)throughout the filtration *** adsorption results demonstrated that the introduction of urethane functional groups on the membranes significantly enhanced the removal efficiency of 17β-estradiol,estriol,bisphenol A,estrone,ethinylestradiol,and *** adsorption loading of 17β-estradiol on the functionalized PES membrane was 6.7±0.7 mg/m^(2),exhibiting a 5-fold increase compared to the unmodified PES *** membranes were successfully regenerated and reused for three adsorption cycles without experiencing any loss of adsorption capacity.

关键词： Surface functionalization Electron beam irradiation Microfiltration Endocrine disrupting chemicals

Fetal MRI Artifacts: Semi-Supervised Generative Adversarial Neural Network for Motion Artifacts Reducing in Fetal Magnetic Resonance Images

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Journal of Computer and Communications 2024年第6期12卷 210-225页

作者： Ítalo Messias Félix Santos Gilson Antonio Giraldi Heron Werner Junior Bruno Richard schulze National Laboratory for Scientific Computing Rio de Janeiro Brazil Biodesign Laboratory Dasa/PUC-Rio Rio de Janeiro Brazil

This study addresses challenges in fetal magnetic resonance imaging (MRI) related to motion artifacts, maternal respiration, and hardware limitations. To enhance MRI quality, we employ deep learning techniques, specifically utilizing Cycle GAN. Synthetic pairs of images, simulating artifacts in fetal MRI, are generated to train the model. Our primary contribution is the use of Cycle GAN for fetal MRI restoration, augmented by artificially corrupted data. We compare three approaches (supervised Cycle GAN, Pix2Pix, and Mobile Unet) for artifact removal. Experimental results demonstrate that the proposed supervised Cycle GAN effectively removes artifacts while preserving image details, as validated through Structural Similarity Index Measure (SSIM) and normalized Mean Absolute Error (MAE). The method proves comparable to alternatives but avoids the generation of spurious regions, which is crucial for medical accuracy.

关键词： Fetal MRI Artifacts Removal Deep Learning Image Processing Generative Adversarial Networks

Exactly Solvable Combinations of Scalar and Vector Potentials for the Dirac Equation Interrelated by Riccati Equations

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Chinese Physics Letters 2006年第6期23卷 1365-1368页

作者： Axel schulze-Halberg Department of Science University of Colima Bernal Diaz del Castillo 340 Col. Villas San Sebastian C.P. 28045 Colima Col. Mexico

New classes of solvable scalar and vector potentials for the Dirac equation are obtained, together with the associated exact Dirac spinors. The method of derivation is based on an a priori constraint between the solutions, leading to an interrelation between the scalar and vector potential in the form ofa Riccati equation. The present note generalizes a series of former articles.

关键词： Exactly Solvable Combinations Riccati Equations

Effect of nanoparticle concentration on zeta-potential measurement results and reproducibility

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Particuology 2010年第3期8卷 279-285页

作者： Ratna Tantra Philipp schulze Paul Quincey National Physical Laboratory Hampton Road Teddington Middlesex TW11 0LW UK Department of Physics University of Bath Bath BA2 7AY UK. National Physical Laboratory Hampton Road Teddington Middlesex TW11 0LW

The effect of nanoparticle concentration on zeta-potential measurement results at dilute concentrations was evaluated. The values of the zeta-potential for four different types of nanoparticles, Ludox （silica）, multi-walled carbon nanotubes （bamboo-shaped and hollow nanotubes） and gold, at various concentrations, were obtained using a laser Doppler electrophoresis instrument. The size of the nanoparticles on dilution was measured using dynamic light scattering （DLS）. The results show that there is a concentration range within which the zeta-potential, and particle size, are not affected by nanoparticle concentration. The lower concentration limit for the system to produce consistent results was dependent on the nature of the sample under study and ranged between 10-2 and 10-4 wt%. Below this concentration, there was an apparent shift in zeta-potential values to less negative values, which was accompanied by an increase in the particle size. The shift in zeta-potential was attributed to an increase in contribution of the signal from extraneous particulate matter. The increase in particle size was attributed to the nature of the homodyne optical configuration of the instrument. The aim of this study was to elucidate the range in nanoparticle concentration that allows for accurate and reliable measurement of the zeta-potential and DLS data.

关键词： Nanoparticles Zeta-potential Dynamic light scattering Reproducibility

^3PF2 neutron superfluidity in neutron stars and three-body force effect

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Chinese Physics B 2008年第9期17卷 3289-3293页

作者：崔常喜左维 H.J.schulze Institute of Modern Physics Chinese Academy of Sciences INFN Sezione di Catania

We investigate the ^3PF2 neutron superfluidity in H-stable neutron star matter and neutron stars by using the BCS theory and the Brueckner-Hartree-Fock approach. We adopt the Argonne V18 potential supplemented with a microscopic three-body force as the realistic nucleon-nucleon interaction. We have concentrated on studying the threebody force effect on the ^3PF2 neutron pairing gap. It is found that the three-body force effect is to enhance remarkably the ^3PF2 neutron superfluidity in neutron star matter and neutron stars.

关键词： ^3PF2 superfluidity neutron star three-body force Brueckner-Hartree-Fock approach

Growth performance,nutrient digestibility,intestinal morphology,cecal mucosal cytokines and serum antioxidant responses of broiler chickens to dietary enzymatically treated yeast and coccidia challenge

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Journal of Animal Science and Biotechnology 2023年第4期14卷 1438-1450页

作者： Emmanuel Oluwabukunmi Alagbe Hagen schulze Olayiwola Adeola Department of Animal Sciences Purdue UniversityWest LafayetteIN 47907USA Livalta PeterboroughCambridgeshire PE26FLUK

Background There is a growing search for natural feed additives to alleviate the deleterious effects of coccidia infection in poultry *** study aimed to investigate the effect of enzymatically treated yeast(ETY) on the growth performance,nutrient digestibility,intestinal morphology,antioxidative status,and cecal mucosa cytokines of coccidia-challenged broiler *** From d 1 to 14 post hatching,480 broiler chickens were allocated to 3 corn-soybean meal-based experimental diets with increasing concentrations of ETY(0,1,or 2 g/kg).The experiment was designed as a randomized complete block design with body weight(BW) used as a blocking *** d 14 post hatching,the birds were re-randomized within each of the 3 experimental *** of the 3 diet groups was split into a challenge or no-challenge *** resulted in a 3 × 2 factorial arrangement of *** coccidia challenge was administered on d 15 by an oral *** Dietary ETY improved(P < 0.05) the G:F of birds on d 21 regardless of the challenge state and linearly increased(P < 0.01) the apparent ileal digestibility of dry matter(DM),nitrogen,and gross energy(GE).The coccidia challenge decreased(P < 0.05) BW gain and feed intake of broiler chickens and reduced(P < 0.01) the total tract retention of DM,GE,and *** coccidia challenge increased(P < 0.01) the mRNA gene expression of TNFα,IL-1β,IL-10,and IL-6 in the cecal *** was a tendency(P ne = 0.08) for ETY to linearly reduce IL-1β ***,ETY supplementation increased(P < 0.05) the geexpression of *** catalase increased(P < 0.05) with dietary ETY in broiler chickens on d *** ETY linearly increased(P < 0.05) the ileal villus height to crypt depth ratio,and ileal goblet cell density in broiler *** ileal and excreta oocyst counts decreased(P < 0.01) with increasing supplementation of dietary ETY in coccidia-challenged broiler chickens on d *** Dietary ETY enha

关键词： Broiler chickens Coccidia Goblet cells Growth performance Health Oocyst Peptides Postbiotics Yeast

Ellipticity on spaces with higher singularities

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Science China Mathematics 2017年第11期60卷 2053-2076页

作者： CHANG Der-Chen schulze Bert-Wolfgang Department of Mathematics and Statistics Georgetown University Department of Mathematics Fu Jen Catholic University Institute of Mathematics University of Potsdam

We study corner-degenerate pseudo-differential operators of any singularity order and develop ellipticity based on the principal symbolic hierarchy, associated with the stratification of the underlying space. We construct parametrices within the calculus and discuss the aspect of additional trace and potential conditions along lower-dimensional strata.

关键词： pseudo-differential operators operator-valued symbols Fourier and Mellin transforms

The Brueckner-Hartree-Fock Equation of State for Nuclear Matter and Neutron Skin

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Chinese Physics Letters 2016年第3期33卷 20-23页

作者：卜庆阳李增花 Hans-Josef schulze Institute of Modern Physics Fudan University Applied Ion Beam Physics Laboratory(Ministry of Eduction) Fudan University INFN Sezione di Catania

The equation of state for nuclear matter is presented within the Brueckner Hartree-Fock （BHF） scheme, by using the realistic Argonne VI8 or Bonn B two-nucleon potentials plus their corresponding microscopic three-nucleon forces. It is then applied to calculate the properties of finite nuclei within a simple liquid-drop model, and we compare the calculated volume, surface, and Coulomb parameters with the empirical ones from the liquid drop model. Nuclear density distributions and charge radii in good agreement with the experimental data are obtained~ and we predict the neutron skin thickness of various nuclei.

关键词： for on of The Brueckner-Hartree-Fock Equation of State for Nuclear Matter and Neutron Skin BHF is been EOS that