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一种胶体金免疫层析试条检测犬利什曼原虫感染的效果评价

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国际医学寄生虫病杂志 2015年第4期42卷 189-192页

作者：冯晓平石锋杨玥涛高春花汪俊云中国疾病预防控制中心寄生虫病预防控制所卫生部寄生虫病原与媒介生物学重点实验室国家级热带病国际联合研究中心世界卫生组织疟疾血吸虫病和丝虫病合作中心

目的评价检测内脏利什曼病特异抗体的胶体金免疫层析试条检测我国动物源型内脏利什曼病流行区犬血样的效果。方法以杜氏利什曼原虫前鞭毛体粗抗原作为包被抗原．以链球菌G蛋白标记胶体金制成检测特异抗体的免疫层析试条（***）。用该试... 详细信息

目的评价检测内脏利什曼病特异抗体的胶体金免疫层析试条检测我国动物源型内脏利什曼病流行区犬血样的效果。方法以杜氏利什曼原虫前鞭毛体粗抗原作为包被抗原．以链球菌G蛋白标记胶体金制成检测特异抗体的免疫层析试条（***）。用该试条检测甘肃省陇南市武都区（动物源型黑热病流行区）和甘肃省漳县（非流行区）犬的腿弯处静脉血样品。同时用镜检法、PCR和酶联免疫吸附试验（enzyme．1inkedimmunosorbentassay，ELISA）分别检测犬骨髓、静脉血和血清样本，并比较各种检测方法的敏感性差异。结果对动物源型内脏利什曼病流行区的105头犬的骨髓涂片镜检，阳性检出率为7．62％（8／105）。应用PCR法的阳性检出率为48．57％（51／105）；检测非流行区30份犬静脉血，1份为阳性，阳性检出率为3-33％（1／30）。试条法和ELISA法检测内脏利什曼病流行区的105份犬血样的阳性检出率分别为32-38％（34／105）和33．33％（35／105）：检测内脏利什曼病非流行区的30份犬血样均为阴性。镜检法的阳性检出率低于PCR法、试条法和EUSA法（x^2=43．58、20．12、21．32，P〈0．05），PCR法的阳性检出率高于镜检法、试条法和EUSA法（x^2=43．58、5．71、5．04，P〈0．05），试条法和EUSA法阳性检出率无统计学差异（x^2=0．02。P〉0．05）。结论快速检测内脏利什曼病的胶体金免疫层析试条适用于检测动物源型内脏利什曼病流行区犬血样中特异性抗体。

关键词：内脏利什曼病免疫层析试条犬检测评价

Design and experiment of fuzzy-PID based tillage depth control system for a self-propelled electric tiller

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International Journal of Agricultural and Biological Engineering 2023年第4期16卷 116-125页

作者： Maohua Xiao Ye Ma Chen wang junyun Chen Yejun Zhu Petr Bartos Guosheng Geng College of Engineering Nanjing Agricultural UniversityNanjing 210031China Faculty of Agriculture University of South BohemiaStudentska 1668Czech

The research on the self-propelled electric tiller is vital for further improving the quality and efficiency of greenhouse rotary tillage operation,reducing the work intensity and operation risk of operators,and achieving environmentally friendly *** of the existing self-propelled tillers rely on manual adjustment of the tillage ***,the consistency and stability of the tillage depth are difficult to *** this study,the automatic control method of tillage depth of a self-propelled electric tiller is investigated.A method of applying the fuzzy PID(Proportional Integral Derivative)control method to the tillage depth adjustment system of a tiller is also proposed to realize automatic *** system uses the real-time detection of the resistance sensor and angle *** controller runs the electronically controlled hydraulic system to adjust the force and position *** fuzzy control algorithm is used in the operation error control to realize the double-parameter control of the tillage *** simulation and experimental verification of the system are *** show that the control system applying fuzzy PID can improve the soil breaking rate by 3%in the operation process based on reducing the stability variation of tillage depth by 24%.The control strategy can reach the set value of tillage depth quickly and *** can also meet the requirement of tillage depth consistency during the operation.

关键词： Fuzzy PID Self-propelled electric tiller Tillage depth Electro-controlled hydraulic system Comprehensive adjustment of force and position

双抗体夹心Dot-ELISA诊断新疆地区皮肤利什曼病

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实用寄生虫病杂志 1996年第4期4卷 149-151页

作者：瞿靖琦中国预防医学科学院寄生虫病研究所

应用双抗体夹心Ｄｏｔ－ＥＬＩＳＡ法诊断新疆皮肤利什曼病患者的循环抗原或抗原—抗体复合物获得较好的结果。实验证明，经皮肤组织病理切片，原虫确诊的３０例患者，２５例为阳性反应（８３．３％）。同时对麻风病、结核病以及疟疾患... 详细信息

应用双抗体夹心Ｄｏｔ－ＥＬＩＳＡ法诊断新疆皮肤利什曼病患者的循环抗原或抗原—抗体复合物获得较好的结果。实验证明，经皮肤组织病理切片，原虫确诊的３０例患者，２５例为阳性反应（８３．３％）。同时对麻风病、结核病以及疟疾患者进行交叉试验，均为阴性。

关键词：皮肤利什曼病 Dot-ELISA 诊断双抗夹心法

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烧结页岩多孔砖长柱偏心受压性能研究

四川建筑科学研究 2018年第2期44卷 67-71页

作者：黄榜彪祁伟伟黄秉章卢强潘佳玉李晓王俊云广西科技大学土木建筑工程学院广西柳州545006 广西科技大学鹿山学院广西柳州545006

为研究偏心率和高厚比对烧结页岩多孔砖砌体长柱偏心受压性能的影响,采用3种偏心率和2种高厚比作为主要变量砌成了6组试件,对其中间部位截面的应变是否符合平截面假设、试件破坏形态和偏心承载力进行了试验研究。结果表明,在偏心率和高... 详细信息

为研究偏心率和高厚比对烧结页岩多孔砖砌体长柱偏心受压性能的影响,采用3种偏心率和2种高厚比作为主要变量砌成了6组试件,对其中间部位截面的应变是否符合平截面假设、试件破坏形态和偏心承载力进行了试验研究。结果表明,在偏心率和高厚比综合影响下,烧结页岩多孔砖砌体长柱能够较好地满足平截面假设。将试验值与按照砌体规范计算出的极限承载力值进行了对比,结果表明,烧结页岩多孔砖砌体长柱偏心率和高厚比有足够的安全储备,按照现行规范方法计算是可行的。

关键词：页岩多孔砖砌体长柱偏心率高厚比

High-throughput transcriptional profiling of perturbations by Panax ginseng saponins and Panax notoginseng saponins using TCM-seq

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Journal of Pharmaceutical Analysis 2023年第4期13卷 376-387页

作者： junyun Cheng Jie Chen Jie Liao Tianhao wang Xin Shao Jinbo Long Penghui Yang Anyao Li Zheng wang Xiaoyan Lu Xiaohui Fan Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang UniversityHangzhou310058China Future Health Laboratory Innovation Center of Yangtze River DeltaZhejiang UniversityJiaxingZhejiang314100China State Key Laboratory of Medical Molecular Biology Department of Biochemistry and Molecular BiologyInstitute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100005China Innovation Center in Zhejiang University State Key Laboratory of Component-Based Chinese MedicineHangzhou310058China Jinhua Institute of Zhejiang University JinhuaZhejiang321016China

Panax ginseng(PG)and Panax notoginseng(PN)are highly valuable Chinese medicines(CM).Although both CMs have similar active constituents,their clinical applications are clearly *** the past decade,RNA sequencing(RNA-seq)analysis has been employed to investigate the molecular mechanisms of extracts or ***,owing to the limited number of samples in standard RNA-seq,few studies have systematically compared the effects of PG and PN spanning multiple conditions at the transcriptomic ***,we developed an approach that simultaneously profiles transcriptome changes for multiplexed samples using RNA-seq(TCM-seq),a high-throughput,low-cost workflow to molecularly evaluate CM perturbations.A species-mixing experiment was conducted to illustrate the accuracy of sample multiplexing in *** from repeated samples were used to verify the robustness of *** then focused on the primary active components,Panax notoginseng saponins(PNS)and Panax ginseng saponins(PGS)extracted from PN and PG,*** also characterized the transcriptome changes of 10 cell lines,treated with four different doses of PNS and PGS,using TCM-seq to compare the differences in their perturbing effects on genes,functional pathways,gene modules,and molecular *** results of transcriptional data analysis showed that the transcriptional patterns of various cell lines were significantly *** exhibited a stronger regulatory effect on genes involved in cardiovascular disease,whereas PNS resulted in a greater coagulation effect on vascular endothelial *** study proposes a paradigm to comprehensively explore the differences in mechanisms of action between CMs based on transcriptome readouts.

关键词： Panax ginseng Panax notoginseng Sample multiplexing RNA sequencing

Human activity and climate change triggered the expansion of rocky desertification in the karst areas of Southwestern China

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Science China Earth Sciences 2021年第10期64卷 1761-1773页

作者： Chaojun CHEN Daoxian YUAN Hai CHENG Tsailuen YU Chuanchou SHEN RLawrence EDWARDS Yao WU Siya XIAO Jian ZHANG Tao wang Ran HUANG Ziqi LIU Tingyong LI junyun LI Chongqing Key Laboratory of Karst Environment School of Geographical SciencesSouthwest UniversityChongqing 400715China Key Laboratory of Karst Dynamics MNRGuangxiInstitute of Karst GeologyChinese Academy of Geological SciencesGuilin 541004China Yunnan Key Laboratory of Plateau Geographical Processes&Environmental Changes Faculty of GeographyYunnan Normal UniversityKunming 650500China Institute of Global Environmental Change Xi’an Jiaotong UniversityXi’an 710049China High-precision Mass Spectrometry and Environment Change Laboratory(HISPEC) Department of GeosciencesTaiwan UniversityTaipei 10617China Research Center for Future Earth Taiwan UniversityTaipei 10617China Global Change Research Center Taiwan UniversityTaipei 10617China Department of Earth Sciences University of MinnesotaMinneapolisMN 55455USA Environnements et Paléoenvironnements Océaniques et Continentaux(EPOC) UMR CNRS 5805Universitéde BordeauxPessac 33600France School of Karst Science Guizhou Normal UniversityGuiyang 550001China

It is conducive to the sustainable development of human beings in karst regions to research the mechanism of karst rocky desertification(KRD)*** the large-scale KRD in southwestern China is caused by climate change or human activities is still *** this study,the evolution of the KRD in southwestern China over the past 2000 years was reconstructed through the high-precisionδ^(13)C record of stalagmites from Shijiangjun(SJJ)Cave,Guizhou Province,***δ^(13)C of the stalagmites from SJJ Cave exhibited heavy values from the Medieval Warm Period(MWP)to the Little Ice Age(LIA).Furthermore,theδ^(13)C records of other stalagmites and tufa from southwestern China also showed the same significant heavy *** the stalagmiteδ^(13)C could record the change of ecological environment,it indicated that the consistent change of the stalagmitesδ^(13)C may record the process of KRD expansion in the karst regions of southwestern *** the MWP,the stronger Asian summer monsoon and the northward movement of the rain belt led to a dry period in southwestern China and a wet period in northern *** contrast,it was wet in southwestern China and dry in northern China during the *** addition,after the Jing-Kang event(JK event,AD1127)occurred at the end of the Northern Song dynasty,the political and economic center of China migrated to southern China for the first time,which changed the population distribution pattern of larger population in the north and smaller population in the ***,the expansion of KRD in southwestern China was exacerbated in the MWP due to the change of climate in southwestern China,the migration of a large number of people,wars,the large-scale reclamation of arable land,and the cultivation of large areas of crops.

关键词： Karst rocky desertification Jing-Kang event Stalagmiteδ13C record Medieval Warm Period Little Ice Age

SiCp/Al复合材料切削过程的颗粒损伤研究

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机械工程学报 2023年第15期59卷 366-376页

作者：孙伟王培军陈俊云段春争燕山大学机械工程学院秦皇岛066004 大连理工大学机械工程学院大连116024

SiCp/Al复合材料动态去除过程中极易发生颗粒损伤,为避免或利用复合材料切削加工过程中的颗粒损伤现象,优化SiCp/Al复合材料切削加工,深入研究了SiCp/Al复合材料切削的颗粒损伤现象及其对切削加工的影响。首先,通过位错塞积理论和切屑... 详细信息

SiCp/Al复合材料动态去除过程中极易发生颗粒损伤,为避免或利用复合材料切削加工过程中的颗粒损伤现象,优化SiCp/Al复合材料切削加工,深入研究了SiCp/Al复合材料切削的颗粒损伤现象及其对切削加工的影响。首先,通过位错塞积理论和切屑根部微观观察,揭示了SiCp/Al复合材料切削的颗粒损伤机理,发现位错塞积引起的应力集中是导致界面脱粘的主因,颗粒断裂主要是由集中应力、刀刃挤压颗粒、局部颗粒聚集挤压以及颗粒连成网状结构引起;然后,基于考虑颗粒影响的动态本构模型、Eshelby等效夹杂理论、Weibull统计分布和刀刃-颗粒接触分析,建立了SiCp/Al复合材料切削的颗粒损伤度模型,并借助图像处理技术验证了模型的有效性;最后,根据颗粒损伤度预测结果,分析了颗粒损伤度对SiCp/Al复合材料切削加工的影响。结果显示,颗粒损伤度增大,会使切屑锯齿化程度增强,并严重降低已加工表面质量;颗粒损伤会显著影响颗粒强化效应,导致切削力随颗粒含量增大先升后降、随颗粒尺寸增大而降低。

关键词： SiCp/Al复合材料切削颗粒损伤机理理论建模

原料液氮低温球磨处理对Ti-Al_2O_3复合材料力学性能的影响

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济南大学学报（自然科学版） 2018年第2期32卷 134-139页

作者：刘倩王志吴俊彦济南大学材料科学与工程学院山东济南250022

针对Ti-Al_2O_3金属陶瓷烧结过程中易形成金属间化合物脆性相,从而劣化力学性能的问题,采用液氮低温球磨制备表面氮钝化的Ti纳米粉体,并进一步制备Ti-Al_2O_3复合材料,研究液氮球磨时间对Ti粉物相组成和微观结构的影响,并对比液氮球磨... 详细信息

针对Ti-Al_2O_3金属陶瓷烧结过程中易形成金属间化合物脆性相,从而劣化力学性能的问题,采用液氮低温球磨制备表面氮钝化的Ti纳米粉体,并进一步制备Ti-Al_2O_3复合材料,研究液氮球磨时间对Ti粉物相组成和微观结构的影响,并对比液氮球磨处理前、后粉体对Ti-Al_2O_3复合材料物相组成、微观结构及其力学性能的影响。结果表明,液氮低温球磨后的复合粉体明显改善了Ti-Al_2O_3复合材料的烧结情况,孔洞减少,致密性变好,物相分散均匀,并且液氮球磨后未发现Ti3Al脆性相,取而代之的是Ti N和Al Ti3N相,从而使Ti-Al_2O_3复合材料的强度和硬度显著增大。

关键词：热压烧结低温球磨 Ti-Al203复合材料

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纳米孪晶立方氮化硼的飞秒激光材料去除机理

机械工程学报 2019年第9期55卷 198-205页

作者：靳田野陈俊云王金虎赵清亮哈尔滨工业大学精密工程研究所燕山大学车辆与能源学院

为实现能应用于超精密切削的新型超硬纳米李晶立方氮化硼(nt-cBN)刀具的制备,探索了飞秒激光烧蚀方法对nt-cBN的材料去除特性。使用激光单点烧蚀方法及理论公式计算出了nt-cBN的烧蚀阈值及阈值功率,分别为0.523 J/cm^2和4.1 mW;基于烧... 详细信息

为实现能应用于超精密切削的新型超硬纳米李晶立方氮化硼(nt-cBN)刀具的制备,探索了飞秒激光烧蚀方法对nt-cBN的材料去除特性。使用激光单点烧蚀方法及理论公式计算出了nt-cBN的烧蚀阈值及阈值功率,分别为0.523 J/cm^2和4.1 mW;基于烧蚀阈值,对nt-cBN材料在多种烧蚀功率下的单点烧蚀圆孔形貌进行了表征,并分析了不同阶段圆孔内壁多种微结构的形成与演变机理,阐明了随激光功率增加,nt-cBN表面经历了快速热汽化、熔化和相爆炸三种材料去除机理,并形成了纳米驼峰、爆裂和周期性波纹等不同烧蚀显微结构;对nt-cBN块材进行了直线扫描烧蚀加工,提出了基于光束扫描的动态烧蚀过程对材料的破坏形式及去除原理,并与单点烧蚀形成对比;建立了不同烧蚀功率下,飞秒激光扫描速度与加工微槽宽度和深度的关系曲线,并分析了烧蚀功率和扫描速度对加工效率和加工质量的影响规律,提出了能够保证加工效率并同时改善加工质量的激光扫描速度在0.1mm/s左右。

关键词：飞秒激光纳米挛晶立方氮化硼烧蚀阈值周期性微结构