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Specific metabolic impairments indicate loss of sustained liver improvements in metabolic dysfunction-associated steatotic liver disease treatment

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Hepatobiliary Surgery and Nutrition 2024年第4期13卷 632-649,I0025-I0035页

作者： Ling Luo junzhao Ye Shuyu Zhuo Bo Ma Weiyi Mai Xiaopei Cao Liuqin Liang Wei Wang Shiting Feng Zhi Dong Bihui Zhong Department of Gastroenterology The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Department of Nutrition The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Department of Gastroenterology The East Division of the First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Department of Cardiology The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Department of Endocrinology The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Department of Rheumatology The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Department of Ultrasound The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Department of Radiology The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina

Background:High liver fat content(LFC)induces increased risks of both hepatic and extrahepatic progression in metabolic dysfunction-associated steatotic liver disease(MASLD),while maintaining a significant decline in magnetic resonance imaging-based proton density fat fraction(MRI-PDFF)(≥30%decline relative to baseline)without worsening fibrosis results in improved histological severity and ***,the factors associated with the loss of sustained responses to treatment remain unclear,and we aim to identify ***:Consecutive treatment-naïve MASLD patients between January 2015 and February 2022,with follow-up until April 2023,were included in this prospective cohort *** quantified by MRI-PDFF and liver stiffness measurements(LSM)determined by two-dimensional shear wave elastography(2D-SWE)were evaluated at weeks 0,24 and ***-PDFF response was defined as a≥30%relative decline in PDFF values,and LSM response was defined as a≥1 stage decline from ***:A total of 602 MASLD patients were *** the 303 patients with a 24-week MRI-PDFF response and complete follow-up of 48 weeks,the rate of loss of MRI-PDFF response was 29.4%,and multivariable logistic regression analyses showed that 24-week insulin resistance(IR),still regular exercise and caloric restriction after 24 weeks,and the relative decline in LFC were risk factors for loss of MRI-PDFF *** of LSM response at 48 weeks occurred in 15.9%of patients,and multivariable analysis confirmed 24-week serum total bile acid(TBA)levels and the relative decline in TBA from baseline as independent *** significant association was found at 48 weeks between loss of MRI-PDFF response and loss of LSM ***:MASLD patients with IR and high TBA levels are at higher risks of subsequent diminished sustained improvements of steatosis and fibrosis,respectively.

关键词： Metabolic dysfunction-associated steatotic liver disease(MASLD) loss of response magnetic resonance imaging-based proton density fat fraction response(MRI-PDFF response) liver stiffness measurements response(LSM response)

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新时期乡镇政府职能转变问题研究

中州学刊 2017年第7期 8-12页

作者：马君昭平顶山学院政法学院行政管理教研室

推进乡镇政府职能转变,对于建设基层服务型政府和实现乡村治理现代化具有十分重要的意义。当前,乡镇政府职能转变过程中面临的主要问题有:"任务型乡镇"行政生态环境并未根本改变,乡镇政府拥有的权力与承担的责任不对等,乡镇政府角色功... 详细信息

推进乡镇政府职能转变,对于建设基层服务型政府和实现乡村治理现代化具有十分重要的意义。当前,乡镇政府职能转变过程中面临的主要问题有:"任务型乡镇"行政生态环境并未根本改变,乡镇政府拥有的权力与承担的责任不对等,乡镇政府角色功能在实践中存在越位和缺位,乡镇干部有效履职和服务的动力不足等。新的历史条件下推进乡镇政府职能转变,需要进一步理顺县乡关系、优化乡镇行政生态环境,提高乡镇政府自主能力、改变权责不对等状况,对乡镇政府角色功能进行恰当定位,完善激励机制、增强乡镇干部转变职能内驱力。

关键词：乡镇政府机构改革政府职能职能转变

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锅炉脱硝装置运行对高背压供热机组影响分析

中国电力 2017年第1期50卷 181-184页

作者：徐君诏华能新疆能源开发有限公司新疆乌鲁木齐830017

近些年由于环境污染问题日趋严峻,城市周边的供热机组锅炉都安装了烟气脱硝装置,而烟气脱硝装置的运行对高背压供热系统有一定的影响。通过对高背压供热系统热力特性分析,得出锅炉最低脱硝负荷是制约高背压供热系统经济运行的主要因素,... 详细信息

近些年由于环境污染问题日趋严峻,城市周边的供热机组锅炉都安装了烟气脱硝装置,而烟气脱硝装置的运行对高背压供热系统有一定的影响。通过对高背压供热系统热力特性分析,得出锅炉最低脱硝负荷是制约高背压供热系统经济运行的主要因素,提出高背压供热机组实现脱硝装置全负荷运行改造方案,以某电厂135 MW高背压供热机组分析,实施改造后将降低供电煤耗8.67 g/(k W·h),进一步挖掘了高背压供热机组的节能降耗潜力。

关键词：火力发电机组高背压供热机组烟气脱硝装置经济性

Mutation analysis of novel human liver-related putative tumor suppressor gene in hepatocellular carcinoma

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World Journal of Gastroenterology 2003年第1期9卷 89-93页

作者： ChengLiao Mu-junzhao JingZhao HaiSong PascalPineau AgnésMarchio AnneDejean PierreTiollais Hong-YangWang Tsai-PingLi StateKeyLaboratoryofMolecularBiology InstituteofBiochemistryandCellBiologyShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai200031China UnitedeRecombinaisonetExpressionGénétique INSERMU163InstitutePasteurParisFrance ShanghaiEastemHepatobiliary SurgeryHospitalShanghai200433China

AIM: To find the point mutations meaningful for inactivationof liver-related putative tumor suppressor gene (LPTS) gene,a human novel liver-related putative tumor suppressor geneand telomerase inhibitor in hepatocellular ***: The entire coding sequence of LPTS genewas examined for mutations by single strand conformationpolymorphism (SSCP) assay and PCR products directsequencing in 56 liver cancer cell lines, 7 ovarian cancerand 7 head & neck tumor cell lines and 70 pairs of HCCtissues samples. The cDNA fragment coding for the mostfrequent mutant protein was subcloned into GST fusionexpression vector. The product was expressed in E. coliand purified by glutathione-agarose column. Telomericrepeat amplification protocol (TRAP) assays wereperformed to study the effect of point mutation totelomerase inhibitory ***: SSCP gels showed the abnormal shifting bandsand DNA sequencing found that there were 5 differentmutations and/or polymorphisms in 12 tumor cell lineslocated at exon2, exon5 and exon7. The main alterationswere A(778)A/G and A(880)T in exon7. The change in siteof 778 could not be found in HCC tissue samples, while themutation in position 880 was seen in 7 (10 %) cases. Themutation in the site of 880 had no effect on telomeraseinhibitory ***: Alterations identified in this study arepolymorphisms of LPTS gene. LPTS mutations occur in HCCbut are infrequent and of little effect on the telomeraseinhibitory function of the protein. Epigenetics, such asmethylation, acetylation, may play the key role in inactivationof LPTS.

关键词：肝细胞癌肿瘤抑制基因端粒酶聚合酶链反应

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铝铜药型罩射流与侵彻数值模拟

高压物理学报 2019年第6期33卷 130-137页

作者：刘志跃翟俊昭北京理工大学爆炸科学与技术国家重点实验室

为提高射流侵彻性能,根据聚能射流装置的射流形成特点,设计了爆炸复合铝铜金属体作为药型罩的聚能射流装置。此装置依据已有的锥角为42°的聚能装药紫铜药型罩改进而来。利用 LS-DYNA 软件中的MMALE 多物质算法,对此装置的射流形成、... 详细信息

为提高射流侵彻性能,根据聚能射流装置的射流形成特点,设计了爆炸复合铝铜金属体作为药型罩的聚能射流装置。此装置依据已有的锥角为42°的聚能装药紫铜药型罩改进而来。利用 LS-DYNA 软件中的MMALE 多物质算法,对此装置的射流形成、侵彻金属靶体全过程进行数值模拟。在保持装药量不变的情况下,计算了当铝铜药型罩锥角分别为 36°、38°、40°和 42°时的射流形成及侵彻过程。结果表明:射流头部速度随着铝铜药型罩锥角的减小而增大;且锥角为 38°时射流穿深最大。相比单纯金属铜药型罩情况,射流头部速度提高了 13.2%,侵彻深度提高了 14.5%。

关键词：聚能射流侵彻铜铝复合药型罩 LS-DYNA

Can SpRY recognize any PAM in human cells?

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2022年第5期23卷 382-391页

作者： Jinbin YE Haitao XI Yilu CHEN Qishu CHEN Xiaosheng LU Jineng LV Yamin CHEN Feng GU junzhao ZHAO Reproduction Center Department of Obstetrics and Gynecologythe Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical UniversityWenzhou 325000China School of Ophthalmology and Optometry Eye HospitalWenzhou Medical UniversityState Key Laboratory and Key Laboratory of Vision ScienceMinistry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and OptometryWenzhou 325000China

The application of clustered regularly interspaced short palindromic repeats(CRISPR)and CRISPR-associated proteins(Cas)can be limited due to a lack of compatible protospacer adjacent motif(PAM)sequences in the DNA regions of ***,SpRY,a variant of Streptococcus pyogenes Cas9(SpCas9),was reported,which nearly completely fulfils the PAM ***,PAMs for Sp RY have not been well *** our previous study,we developed the PAM Definition by Observable Sequence Excision(PAM-DOSE)and green fluorescent protein(GFP)-reporter systems to study PAMs in human ***,we endeavored to identify the PAMs of SpRY with these two *** results indicated that 5’-NRN-3’,5’-NTA-3’,and 5’-NCK-3’could be considered as canonical PAMs.5’-NCA-3’and 5’-NTK-3’may serve as non-priority *** the same time,PAM of 5’-NYC-3’is not recommended for human *** findings provide further insights into the application of SpRY for human genome editing.

关键词： CRISPR/Cas SpRY Protospacer adjacent motif(PAM) Recognize

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党政干部失责必问常态化研究

云南行政学院学报 2019年第3期21卷 86-91页

作者：马君昭平顶山学院政法学院

失责必问常态化是对失责党政干部必须问责的经常性管理过程。责、权、利相结合是政治学的一条基本原则,它要求拥有一定的权力必须承担一定的责任。中国社会转型期的特征、相关的法律法规为失责必问提供了现实依据和制度依据。完善失责... 详细信息

失责必问常态化是对失责党政干部必须问责的经常性管理过程。责、权、利相结合是政治学的一条基本原则,它要求拥有一定的权力必须承担一定的责任。中国社会转型期的特征、相关的法律法规为失责必问提供了现实依据和制度依据。完善失责必问常态化机制需要发挥正式制度的强制性作用,塑造问责文化,发挥异体问责的功能,做好失责必问的动态管理。

关键词：党政干部失责必问常态化正式制度

Cloning of human 15ku selenoprotein gene from H9 T cells

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World Journal of Gastroenterology 2003年第8期9卷 1777-1780页

作者： Ke-JunNan Chun-LiLi Yong-ChangWei Chen-GuangSui ZhaoJing Hai-XiaQin Li-junzhao Bo-RongPan DepartmentofMedicalOntology.FirstHospitalofXi'anJiaot0ngUniversity Xi’an710061ShaanxiProvinceChina TheUniversityofGeorgia AthensGa30602USA DepartmentofOncology XijingHospitalFourthMilitaryMedicalUniversityXi'an710032ShaartxiProvinceChina

AIM: To clone human 15ku selenoprotein ***: H9 human T cells were cultured in RPMI1640medium supplemented with 100 mL/L fetal calf serum. mRNA was isolated from the cells. cDNA library was constructed by RT-PCR. The human 15ku selenoprotein gene was obtained by PCR and cloned into T vector and ***: A unique cDNA fragment about 1 244 bp was obtained. Sequence analysis identified an open reading frame within the cDNA. The gene had an in-frame TGA, which encoded selenocysteine (Sec), and a 3′-UTR SECIS element,which was required for synthesis of selenoprotein. The predicted protein molecular mass was about 15ku (162residues). The result was identical with human liver 15ku selenoprotein gene published in ***: Human 15ku selenoprotein gene can be successfully obtained from T cell line.

关键词：硒蛋白基因 T细胞聚合酶链反应 cDNA文库

基于半隐式格式的水平集法连续体结构形状和拓扑优化方法

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固体力学学报 2008年第2期29卷 175-180页

作者：罗俊召陈立平罗震华中科技大学机械学院CAD中心武汉430074

提出一种改进的基于水平集方法的结构拓扑优化方法.将半隐式的加性算子分裂方法(AOS)引入传统的水平集方程求解,使得原来的Hamilton-Jacobi偏微分方程摆脱了差分法中CFL条件对时间步长的严格限制,差分格式变得高效且稳定.在求解水平集... 详细信息

提出一种改进的基于水平集方法的结构拓扑优化方法.将半隐式的加性算子分裂方法(AOS)引入传统的水平集方程求解,使得原来的Hamilton-Jacobi偏微分方程摆脱了差分法中CFL条件对时间步长的严格限制,差分格式变得高效且稳定.在求解水平集方程过程中不用再对高维的水平集函数进行耗时的周期性的初始化,这样解决了传统水平集方法在优化过程中不能生成新孔的问题.通过典型算例验证了该文算法的有效性.

关键词：拓扑优化水平集法加性算子分裂有限差分法