检索结果-南通市图书馆

The Viral Load of Epstein-Barr Virus in Blood of Children after Hematopoietic Stem Cell Transplantation

Biomedical and Environmental Sciences 2022年第9期35卷 804-810页

作者： wang Wen jun FENG Shun Qiao HE Feng DU Hai jun FENG Miao wang Rui Fang mei Guo Yong LIU Mi LIU Rong YAO Hai Lan HAN jun State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Department of Hematology Children’s Hospital of Capital Institute of PediatricsBeijing 100020China Department of Biochemistry&Immunology Capital Institute of PediatricsBeijing 100020China

Objective To detect the Epstein-Barr virus(EBV)viral load of children after hematopoietic stem cell transplantation(HSCT)using chip digital PCR(cdPCR).Methods The sensitivity of cdPCR was determined using EBV plasmids and the EBV B95-8 *** specificity of EBV cdPCR was evaluated using the EBV B95-8 strain and other herpesviruses(herpes simplex virus 1,herpes simplex virus 2,varicella zoster virus,human cytomegalovirus,human herpesvirus 6,and human herpesvirus 7).From May 2019 to September 2020,64 serum samples of children following HSCT were *** infection and the viral load of serum samples were detected by *** epidemiological characteristics of EBV infections were analyzed in HSCT *** The limit of detection of EBV cdPCR was 110 copies/mL,and the limit of detection of EBV quantitative PCR was 327 copies/mL for the pUC57-BALF5 *** result of EBV cdPCR was up to 121 copies/mL in the EBV B95-8 strain,and both were more sensitive than that of quantitative *** cdPCR,the incidence of EBV infection was 18.75%in 64 children after *** minimum EBV viral load was 140 copies/mL,and the maximum viral load was 3,209 copies/mL using *** average hospital stay of children with EBV infection(184±91 days)was longer than that of children without EBV infection(125±79 days),P=*** EBV cdPCR had good sensitivity and *** incidence of EBV infection was 18.75%in 64 children after HSCT from May 2019 to September *** cdPCR could therefore be a novel method to detect EBV viral load in children after HSCT.

关键词： Chip digital PCR Epstein-Barr virus Hematopoietic stem cell transplantation Quantitative PCR

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Permeabilization of Escherichia coli with ampicillin for a whole cell biocatalyst with enhanced glutamate decarboxylase activity

引用

Chinese Journal of Chemical Engineering 2016年第7期24卷 909-913页

作者： Weirui Zhao Sheng Hu jun Huang Piyu Ke Shanjing Yao Yinlin Lei Lehe mei Jinbo wang School of Biotechnology and Chemical Engineering Ningbo Institute of Technology Zhejiang University Ningho 315100 China Department of Chemical and Biological Engineering Zhejiang University Hangzhou 310027 China School of Biological and Chemical Engineering Zhejiang University of Science and Technology Hangzhou 310023 China

The activity of whole-cell biocatalysts is strongly compromised by the cell envelope, which is a permeability harrier against the diffusion of substrates and products. Although common chemical or physical permeahilization methods used in cultured cells enhance cell permeability, these methods inevitably add several extra processing steps after cell cultivation, as well as impede large scale processing. To increase membrane permeability and cell- bound glutamate decarboxylase （GAD） activity of recombinant Escherichia coil （BL21 （DE3）-pET28a-gadB） cells without the need for an additional permeabilization step, we investigated the permeabilizing effects of adding cell wall synthesis inhibitors or suffactants to the culture media. Ampidllin was the most effective at improving cell-bound GAD activity of the BL21 （DE3）-pET28a-gadB, although it decreased the cell biomass yield. The best permeabilization effect was observed using an ampicillin concentration of 5 pg. ml-1. Using this concentration, the cell hiomass did decrease by 40.58%, but the cell-bound GAD activity of BL21 （DE3）-pET28a-gadB and total cell-bound GAD activity per milliliter of culture was enhanced by 6.24- and 3.64-fold, respectively. Treatment ofBL21 （DE3）-pET28a-gadB cells with 5 *** 1 ampicillin resulted in structural changes to the cell envelope, but did not substantially affect GAD expression. By entrapping the ampicillin-treated cells in an open pore gelation matrix, which is a polymer derived from polyvinyl alcohol （PVA）, alginate, and boric acid, the transfor- mation rate of γ-aminobutyric acid （GABA） at the 10th cycle produced by immobilized and permeabilized cells remained 46% of the first cycle. GAD activity of the immobilized, permeabilized cells remained over 90% after 30 days of storage at 4 ℃.

关键词： γ-Aminobutyric acidAmpicillinEscherichio. coilGlutamate decarboxylasePermeabilization

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cytoskeleton in Pollen and Pollen Tubes of Ginkgo biloba L.

引用

Journal of Integrative Plant Biology 2005年第8期47卷 952-958页

作者： jun-mei LIU Hong ZHANG Yan LI State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beijing 100094 China Department of Horticulture Hebei Agricultural University Baoding 077001 China

The distribution of F-actin and microtubules was investigated in pollen and pollen tubes of Ginkgo biloba L. using a confocal laser scanning microscope after fluorescence and immunofluorescence labeling. A dense F-actin network was found in hydrated Ginkgo pollen. When Ginkgo pollen was germinating, F-actin mesh was found under the plasma membrane from which the pollen tube would emerge. After pollen germination, F-actin bundles were distributed axially in long pollen tubes of G. biloba. Thick F-actin bundles and network were found in the tip of the Ginkgo pollen tube, which is opposite to the results reported for the pollen tubes of some angiosperms and conifers. In addition, a few circular F-actin bundles were found in Ginkgo pollen tubes. Using immunofluorescence labeling, a dense microtubule network was found in hydrated Ginkgo pollen under confocal microscope. In the Ginkgo pollen tube, the microtubules were distributed along the longitudinal axis and extended to the tip. These results suggest that the cytoskeleton may have an essential role in the germination of Ginkgo pollen and tube growth.

关键词： F-actin Ginkgo biloba microtubules pollen tube.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

湖北省文旅产业耦合发展评价及时空演化特征研究

引用

黄冈师范学院学报 2025年第1期45卷 109-117页

作者：尹建军刘伟中王巧刘美伦黄冈师范学院地理与旅游学院湖北黄冈438000 北京联合大学应用文理学院北京100191 福建农林大学经济与管理学院福建福州350002 湖南师范大学旅游学院湖南长沙410081

文旅产业高质量融合发展,对新时代提升旅游业发展效率、推动文化供给侧结构性改革、改善文化产业内生增长动力不足等意义重大。以湖北省为研究靶向,构建文化产业和旅游产业两大指标体系,选取2010—2021年相关指标数据,利用熵值法确定指... 详细信息

文旅产业高质量融合发展,对新时代提升旅游业发展效率、推动文化供给侧结构性改革、改善文化产业内生增长动力不足等意义重大。以湖北省为研究靶向,构建文化产业和旅游产业两大指标体系,选取2010—2021年相关指标数据,利用熵值法确定指标权重,计算综合发展效益值,借助耦合度与耦合协调度模型,测度文旅产业融合发展耦合度和耦合协调度,并分析12年来的时空演化特征。研究发现:1.研究期内,湖北省各地市州文化产业与旅游产业综合发展效益值整体呈现增长态势,且时空异质性显著,受2019年底暴发的新冠疫情的冲击,二者综合效益下滑明显;2.湖北省文化产业与旅游产业耦合效应整体处于中等水平,呈现由“低发展水平-低耦合度”向“中等发展水平-中等耦合度”再向“中高发展水平-较高耦合度”演变的趋势,疫情影响下的耦合效应呈现“中低发展水平-较高耦合度”的扭曲发展态势;3.文化产业与旅游产业耦合协调度持续增长,空间上呈中西部协调度低,东部协调度较高空间格局。研究建议:完善文旅顶层设计,加强政府高位引导;重视专业人才培养,强化产业队伍建设;塑造文旅产业品牌,助推产业集群形成;丰富产业科技内涵,提高文旅融合深度。

关键词：文化产业旅游产业耦合评价时空演化湖北省

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

High Performance Humidity Sensor Based on Electrospun Zr_(0.9)Mg_(0.1)O_(2−δ) Nanofibers

引用

Chinese Physics Letters 2012年第11期29卷 27-30页

作者： SU mei-Ying wang Jing YAO Peng-jun DU Hai-Ying School of Electronic Science and Technology Dalian University of TechnologyDalian 116023 Institute of Microelectronics of Chinese Academy SciencesBeijing 100029 School of Educational Technology Shenyang Normal UniversityShenyang 110034 Department of Electromechanical Engineering&Information Dalian Nationalities UniversityDalian 116600

Zr_(0.9)Mg_(0.1)O_(2−δ) nanofibers and ZrO_(2) nanofibers are synthesized using electrospinning and the calcination *** nanofibers are characterized using x-ray diffraction(XRD),a field emission scanning electron microscope(FE-SEM),and a Brunauer–Emmett–Teller(BET)surface *** humidity sensing properties of Zr_(0.9)Mg_(0.1)O_(2−δ) nanofiber sensors are analyzed and compared with those of ZrO_(2) nanofiber *** Zr_(0.9)Mg_(0.1)O_(2−δ) nanofiber humidity sensors exhibit a broader humidity range of 11–97%relative humidity(RH),good linearity,small humidity hysteresis,and rapid response and recovery *** complex impedance plots of the Zr_(0.9)Mg_(0.1)O_(2−δ) sensor at different RHs are drawn,and the humidity sensing mechanism is discussed via an equivalent circuit.

关键词： fibers humidity fiber

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A Study on Anti-oxidative Activity of Soybean Peptideswith Linoleic Acid Peroxidation Systems

引用

Chemical Research in Chinese Universities 2006年第2期22卷 205-208页

作者： XU Li LI Hong-mei HUANG Yi-bing wang Hua NIE Guang-jun ZHANG Xue-zhong Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education Jilin University Changchun 130021 P. R. China College of Food Engineering Jilin Agricultural University Changchun 130018 P. R. China China-Japan Union Hospital of Jilin University Changchun 130033 P. R. China

Soybean bioactive peptides（SBPs） were prepared from the isolated soybean protein by proteolysis with an alkaline protease, alcalase, at 50℃ and pH = 8.0. The dependence of hydrolysis time on hydrolysis degree and molecular weight distribution were examined. The hydrolysate was fractionated on a Sephadex G-25 column and the anti-oxidative activities of the fractions were detected by the method of pyrogallol auto-oxidation. The average chain length of soybean peptides that have anti-oxidative activity was estimated to be about 7. The anti-oxidative properties of the soy- bean peptide were also studied by using linoleic acid peroxidation systems. The optimal condition of the peroxidation system was set up, Vc/Cu^2 ＋ as the inducer at pH = 7.4 and 25 ℃. In addition, soybean peptides show higher antioxidative activity compared with GSH.

关键词： Soybean bioactive peptides Anti-oxidative activity Linoleic acid peroxidation system

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Identifying factors that influence soil heavy metals by using categorical regression analysis:A case study in Beijing,China

引用

Frontiers of Environmental Science & Engineering 2020年第3期14卷 1-14页

作者： jun Yang Jingyun wang Pengwei Qiao Yuanming Zheng junxing Yang Tongbin Chen mei Lei Xiaoming Wan Xiaoyong Zhou Center for Environmental Remediation Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Research Center for Eco-Environmental Sciences Chinese Academy of SciencesBeijing 100085China Beijing Key Laboratory of Remediation of Industrial Pollution Sites Environmental Protection Research Institute of Light IndustryBeijing 100048China

Identifying the factors that influence the heavy metal contents of soil could reveal the sources of soil heavy metal *** this study,a categorical regression was used to identify the factors that influence soil heavy ***,environmental factors were associated with soil heavy metal data,and then,the degree of influence of different factors on the soil heavy metal contents in Beijing was analyzed using a categorical *** results showed that the soil parent material,soil type,land use type,and industrial activity were the main influencing factors,which suggested that these four factors were important sources of soil heavy metals in *** addition,population density had a certain influence on the soil Pb and Zn *** distribution of soil As,Cd,Pb,and Zn was markedly influenced by interactions,such as traffic activity and land use type,industrial activity and population *** spatial distribution of soil heavy metal hotspots corresponded well with the influencing factors,such as industrial activity,population density,and soil parent *** this study,the main factors affecting soil heavy metals were identified,and the degree of their influence was ranked.A categorical regression represents a suitable method for identifying the factors that influence soil heavy metal contents and could be used to study the genetic process of regional soil heavy metal pollution.

关键词： Soil Heavy metal Influencing factor Categorical regression Identification method

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hepatitis D virus dual-infection among Chinese hepatitis B patient related to hepatitis B surface antigen,hepatitis B virus DNA and age

引用

World Journal of Gastroenterology 2023年第38期29卷 5395-5405页

作者： jun Zi Yu-Huan Li Xiao-mei wang Hong-Qin Xu Wen-Hui Liu Jia-Yue Cui jun-Qi Niu Xiu-mei Chi Gene Therapy Laboratory Center for Pathogen Biology and Infectious DiseasesFirst Hospital of Jilin UniversityChangchun 130061Jilin ProvinceChina Department of Hepatology Center for Pathogen Biology and Infectious DiseasesFirst Hospital of Jilin UniversityChangchun 130061Jilin ProvinceChina Department of Histology and Embryology College of Basic Medical SciencesJilin UniversityChangchun 130061Jilin ProvinceChina

The screening practices for hepatitis D virus(HDV)are diverse and nonstandardized worldwide,and the exact prevalence of HDV is *** To estimate HDV prevalence and investigate viral marker quantity trends in patients with hepatitis *** We collected 5594 serum samples from patients with hepatitis B in Jilin Province,China(3293 males and 2301 females,age range of 2 to 89 years).We then conducted tests for hepatitis B surface antigen(HBsAg),hepatitis B Virus(HBV)DNA,anti-hepatitis D antigen(HDAg),and HDV *** We found that the prevalence of anti-HDAg and HDV RNA among hepatitis B patient were 3.6%(3.2-4.2%)and 1.2%(0.9-1.5%),respectively,87.69%of hepatitis D patients were 51-70 years *** infection screening positive rate of patients with HBV DNA levels below 2000 IU/mL(2.0%)was higher than those above 2000 IU/mL(0.2%).Among anti-HDAg positive patients,the HDV RNA positive rate was positively correlated with the HBsAg level and anti-HDAg *** was a weak correlation between HBsAg and anti-HDAg levels among hepatitis D *** Our study highlights the importance of considering multiple factors when assessing the severity of HDV infection,comprehensive evaluation of patients’clinical and laboratory parameters is necessary for proper diagnosis and treatment.

关键词： Hepatitis D virus Hepatitis B virus Epidemiology Anti-hepatitis D antigen Hepatitis D virus RNA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synthesis of Small-sized Coesite Under Lower Pressure

引用

Chemical Research in Chinese Universities 2010年第3期26卷 352-354页

作者： wang De-jun LIU Xiao-mei ZHU Cheng-jun YAN Duan-ting LIU Fu-lin SU Wen-hui State Key Laboratory of Superhard Materials College of Physics Jilin University Changchun 130021 P R. China Adult Education College of Changchun University Changchun 130022 P. R. China Center of the Condensed Matter Science and Technology Harbin Institute of Technology Harbin 150001 P R. China International Centre for Materials Physics Chinese Academy of Sciences Shenyang 110016 P. R. China

With the help of high-energy mechanical milling and a-SiO2 as the initial material, we investigated the synthesis of coesite at a high temperature and high pressure under the condition of adding a certain amount of hard Fe filling. The synthetic samples were measured by XRD and Raman spectroscopy. The results show that a small amount of small-sized coesite can be obtained under 2.5 GPa and 973 K.

关键词： Coesite High temperature and high pressure High-pressure micro-area a-SiO2

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

MoO_(3)/C-supported Pd nanoparticles as an efficient bifunctional electrocatalyst for ethanol oxidation and oxygen reduction reactions

引用

Rare Metals 2023年第5期42卷 1516-1525页

作者： mei-Ling wang Jin Zhao Jin-Jin wang jun-Ming Zhang Yu-Zhu Tian Zhi-Zhu Yue Dong Li Tian-jun Hu Jian-Feng Jia Hai-Shun Wu Key Laboratory of Magnetic Molecules and Magnetic Information Materials of Ministry of Education School of Chemistry and Materials ScienceShanxi Normal UniversityTaiyuan030006China Research Institute of Materials Science Shanxi Normal UniversityTaiyuan030006China

metal oxide electronic interactions in composite electrocatalysts have a considerable impact on their catalytic *** this study,we successfully synthesized an electrocatalytic material composed of MoO_(3)/C speciessupported Pd nanoparticles(Pd-MoO_(3)/C)using a convenient hydrothermal method,which exhibited excellent catalytic activities for both ethanol oxidation and oxygen reduction in KOH *** specific activity of PdMoO_(3)/C toward ethanol oxidation with MoO_(3)loading(40wt%)was~2.6 times greater than that for the commercial Pd/C(10 wt%)with the same Pd *** particular,the activity could effectively hold up to~60%of its maximum activity after 500-cycle tests,demonstrating improved cyclical ***,the fast electron transfer kinetics toward oxygen reduction for Pd-MoO_(3)/C(40%)were also comparable to those of commercial Pt/C(20 wt%)*** superior electrochemical features are primarily derived from the stronger electronic coupling between Pd and MoO_(3)through charge transfer,which can supply more active centers and improve the anti-poisoning ***,the MoO_(3)species in the Pd-MoO_(3)/C composite may provide additional benefits in terms of electrical conductivity and dispersion.

关键词： Bifunctional electrocatalyst Ethanol oxidation reaction(EOR) metal oxide electronic interaction MoO_(3)species Oxygen reduction reaction(ORR)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：