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Side-stream smoking reduces intestinal inflammation and increases expression of tight junction proteins

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World Journal of Gastroenterology 2012年第18期18卷 2180-2187页

作者： Hui Wang jun-xing zhao Nan Hu jun Ren Min Du Mei-jun Zhu Department of Animal Science University of Wyoming Laramie WY82071 United States School of Pharmacy University of Wyoming Laramie WY 82071 United States

AIM:To investigate the effect of side-stream smoking on gut microflora composition,intestinal inflammation and expression of tight junction ***:C57BL/6 mice were exposed to side-stream cigarette smoking for one hour daily over eight *** contents were collected for microbial composition *** intestine was collected for immunoblotting and quantitative reverse transcriptase polymerase chain reaction analyses of the inflammatory pathway and tight junction ***:Side-stream smoking induced significant changes in the gut microbiota with increased mouse intestinal bacteria,Clostridium but decreased Fermicutes(Lactoccoci and Ruminococcus),Enterobacteriaceae family and Segmented filamentous baceteria compared to the control ***,side-stream smoking inhibited the nuclear factor-κB pathway with reduced phosphorylation of p65 and IκBα,accompanied with unchanged mRNA expression of tumor necrosis factor-α or *** contents of tight junction proteins,claudin3 and ZO2 were up-regulated in the large intestine of mice exposed side-stream *** addition,side-stream smoking increased c-jun N-terminal kinase and p38 MAPK kinase signaling,while inhibiting AMPactivated protein kinase in the large ***:Side-stream smoking altered gut microflora composition and reduced the inflammatory response,which was associated with increased expression of tight junction proteins.

关键词： Inflammation Microbiota Tight junction protein Side-stream smoking Intestine

Protective Efficacy of Traditional Chinese Medicine Compound against Colibacillosis in Broilers

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Animal Husbandry and Feed Science 2011年第2期3卷 25-26页

作者： SHI Shu-jun zhao xing-hua DU Jian College of Traditional Chinese Veterinary Medicine Agricultural University of Hebei Dingzhou 073000 China

[Objective] The aim of the research was to evaluate the protective efficacy of traditional Chinese medicine （TCM） compound against chicken colibacillosis. [Method] Two TCM preparations were obtained by modifying the proved recipes. The AA broilers were selected and chal- lenged by E. coli. Antibacterial test in vitro and protection test in vivo were conducted to investigate the protective efficacy of the two TCM prepara- tions A and B against chicken colibacillosis. [ Result] The mortality of chickens treated with the prescription A or B was significantly lower than that of the control. The protective rates of the prescription A and B were respectively 72.1% and 81.4%, both higher than that of the control （32.5%）. E Conclusion] The TCM compound A and B can be used to protect broilers from colibacillosis.

关键词： Chinese herbal E. coli Broilers Prevention

Construction and Preliminary Analysis of Ovine Oocytes Proteomic 2-DE Map

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Animal Husbandry and Feed Science 2011年第4期3卷 1-3页

作者： LIN Yong-feng ZHANG Yong LI Hong-yan HU jun-jie zhao xing-xu College of Veterinary Medicine Gansu Agricultural University Lanzhou 730070 China

[Objective] To obtain ovine oocytes 2-dimensional gel electrophoresis （2-DE） maps. [Method] Ovine oocytes were solubilized in lysis buffer, and protein concentrations were measured using the method of Bradford and Ramagli. Then the samples were subjected to two-dimensional polyacrylamide gel electrophoresis. The 2-DE maps of ovine oocytes were analyzed through PDQuest 8.0. [Resait] The Ramagli method could re- flect concentration of oocyte protein more actually and could optimize the quantity of samples to get a 2-DE map with higher quality. The protein concentration measured by the Bradford method was higher than its actual value. [Coclusion] Each 2-DE map loading 700 oocytes is more reasonable.

关键词： Ovine Oocytas Two-dimensional polyacrylamide gel electrophoresis Proteome

苦黄注射液治疗病毒性肝炎的有效性和安全性:随机对照试验的系统评价和Meta分析

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中国中药杂志 2017年第20期42卷 4007-4026页

作者：赵君廖星赵晖杨静邹雯王乐支英杰中国中医科学院中医临床基础医学研究所北京100700 中国中医科学院北京100700 中国中医科学院研究生院北京100700

系统评价苦黄注射液治疗病毒性肝炎的有效性和安全性。系统全面检索国内外8大电子数据库及Clinical Trials,收集关于苦黄注射液治疗病毒性肝炎的随机对照试验。根据Cochrane Handbook 5.1评价标准,2人进行文献筛选、资料提取和质量评价... 详细信息

系统评价苦黄注射液治疗病毒性肝炎的有效性和安全性。系统全面检索国内外8大电子数据库及Clinical Trials,收集关于苦黄注射液治疗病毒性肝炎的随机对照试验。根据Cochrane Handbook 5.1评价标准,2人进行文献筛选、资料提取和质量评价。对最终纳入的文献采用Rev Man 5.3软件进行Meta分析或仅做描述性分析。共计纳入32篇文献,涉及3 188例患者,男性1 951例(61.2%),女性859例(26.9%),未知男女比例378例(11.9%),所纳入的临床研究总体质量偏低。由于研究疾病复杂,干预措施不尽相同,大部分研究按疾病分类进行描述性分析:重度黄疸型病毒性肝炎的试验组总有效率优于对照组;黄疸型肝炎试验组在退黄、肝功能复常率方面优于对照组;在少数Meta分析中,对于黄疸性病毒性肝炎,在苦黄注射液+综合治疗组对比综合治疗组中,前者总有效率高于后者(RR=1.35,95%CI=[1.10,1.66],P=0.61)。另外,当苦黄注射液滴速过快时会出现头晕、心慌、恶心呕吐及皮疹等不良反应,减慢滴速可缓解。根据现有证据,苦黄注射液对病毒性肝炎有一定的疗效,但由于大部分研究未对病毒性肝炎进行病原学、临床表现分类以及干预措施多样性,导致临床异质性较大,各研究间的可比性较差,加之研究质量普遍偏低,影响结果的准确性,需更多设计严紧、高质量、多中心随机双盲对照试验以增加证据强度。

关键词：苦黄注射液病毒性肝炎黄疸系统评价

Petrogenesis and tectonic setting of Triassic granitoids in the Qiangtang terrane,central Tibet:Evidence from U–Pb ages,petrochemistry and Sr–Nd–Hf isotopes

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Petrogenesis and tectonic setting of Triassic granitoids in ...

Single-cell omics: experimental workflow, data analyses and applications

中国科学院地质与地球物理研究所2015年度（第15届）学术年会

作者： Guang-Ming Li Jin-Xiang Li jun-xing zhao Ke-Zhang Qin Ming-Jian Cao Noreen J.Evans Key Laboratory of Mineral Resources Institute of Geology and GeophysicsChinese Academy of Sciences CAS Center for Excellence in Tibetan Plateau Earth Sciences Key Laboratory of Continental Collision and Plateau Uplift Institute of Tibetan Plateau ResearchChinese Academy of Sciences CSIRO Earth Science and Resource Engineering John de Laeter Center for Isotope Research Dept.Applied GeologyCurtin University

Triassic granitoids,including the$220 Ma Shuanghu and$210 Ma Rongma granitoids studied here,are widely distributed around the Longmu–Shuanghu suture and in the Qiangtang terrane,central *** majority of these granitoids can be classified as high-K calc-alkaline in nature and yield negative Ba and Sr anomalies on primitive mantle-normalized *** addition,they are:enriched in light rare earth elements(LREE)((La/Yb)N=1.61–21.79);strongly enriched in large ion lithophile elements(LILE:e.g.,Cs,Rb,and K),and depleted in high field strength elements(HFSE:e.g.,Nb and Ti).Magma mixing played a role in the genesis of the Shuanghu granodiorites,as indicated by the occurrence of dioritic enclaves and the wide range in zircon Hf compositions(e Hf(t)=à15.0 toà2.5).The I-type Shuanghu granodiorites and S-type Shuanghu and Rongma granites might have been derived from melting of southern Qiangtang crust given the high initial Sr(0.7131–0.7272),low e Nd(t)(à8.9 toà11.1)and zircon e Hf(t)values(à15 toà7.2).The granitoids may have formed during melting of southern Qiangtang crust,heated by upwelling asthenosphere mantle,a result of break-off and delamination of the Paleo-Tethys slab in a collisional setting.

关键词： Geochronology Zircon Hf isotopes Granitoids Qiangtang terrane Tibet

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei Gu Xin Shao Qinqin Wang Xin Dong Bin Duan Feiyang xing jun Wu Minmin Xiao Fangqing zhao Jing-Dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

Study of signal pathway of histamine H3 receptor on AtT-20 cell

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pH=5时水/乙二醇不同体积比对LiFePO_4/C电化学性能的影响

2002全国青年药理学英文学术会议

作者： XIE Jian-jun, LUO Xiao-xing, zhao De-hua Department of Pharmacology, Fourth Military Medical University, 710032, Xi’an, China

AIM To investigate the signal pathway of histamine H receptors. METHOD Radio immunoassay assay was performed to detect ACTH levels in the supematants of AtT-20 cells after administrating reagents and different extracellular culture solutions with or without Ca;IP measured by [H]-inosital and anion exchanger chromatography;cAMP, cGMP, by radio immunoassay;PKC on the membrane, iNO, by immunohistorical essay;intracellular calcium, by laser confocal imaging microscopy. RESULTS (1)Histamine (HA)

关键词： histamine H3 receptor AtT-20 cell signal pathway R-( α )-methylhistamine

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兰州理工大学学报 2017年第5期43卷 69-72页

作者：冯旺军赵星郑文谦刚骏涛曹悦兰州理工大学理学院甘肃兰州730050 兰州理工大学有色金属先进加工与再利用省部共建国家重点实验室甘肃兰州730050

使用不同体积比的水/乙二醇作为溶剂,pH调至5,通过水热法制备出纯相的LiFePO_4,将其与质量分数10%的葡萄糖混合烧结,得到了含碳量不同的LiFePO_4/C材料.对所得产物进行XRD,SEM,TEM以及电化学性能测试,研究了具有不同形貌的产物对其的电... 详细信息

使用不同体积比的水/乙二醇作为溶剂,pH调至5,通过水热法制备出纯相的LiFePO_4,将其与质量分数10%的葡萄糖混合烧结,得到了含碳量不同的LiFePO_4/C材料.对所得产物进行XRD,SEM,TEM以及电化学性能测试,研究了具有不同形貌的产物对其的电化学性能的影响.结果表明不同形貌的LiFePO_4/C材料的电化学性能差异较大,其中pH=5条件下,水/乙二醇体积比为1∶1时材料的放电比容量最好,0.1C倍率下首次放电比容量为146mAh/g,充放电循环50次后,放电比容量没有明显的衰减,10C倍率下放电比容量为68mAh/g,充放电循环50次后,容量未见明显的衰减.

关键词：碳包覆 LiFePO4/C 形貌电化学性能

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壳聚糖复合创面敷料研究进展

药物生物技术 2017年第4期24卷 369-372页

作者：李玉寒卞君醒狄斌赵霞李博中国药科大学药物分析系中国药科大学蛋白质化学与结构生物学重点实验室江苏南京210009 南京师范大学生命科学学院江苏南京210000 无锡市药品不良反应监测中心江苏无锡214028

壳聚糖因具有止血、良好的生物相容性和生物降解性、诱导细胞增殖和组织修复以及抗菌等生物活性而被广泛用于创面治疗。虽然壳聚糖具有作为生物敷料的优势,但机械强度低,抗菌效果不稳定,促创面愈合时间长等缺陷限制了它的临床应用。壳... 详细信息

壳聚糖因具有止血、良好的生物相容性和生物降解性、诱导细胞增殖和组织修复以及抗菌等生物活性而被广泛用于创面治疗。虽然壳聚糖具有作为生物敷料的优势,但机械强度低,抗菌效果不稳定,促创面愈合时间长等缺陷限制了它的临床应用。壳聚糖复合创面敷料通过在壳聚糖基质上负载或交联其他材料或药物,可提高敷料的机械强度,加速细胞增殖和伤口愈合,增强抗菌活性。文章针对近几年的壳聚糖及其衍生物的复合型创面敷料表现的优势特性开展了综述,并对其进一步研究方向也进行了展望。

关键词：壳聚糖机械强度抗菌活性细胞增殖创面愈合复合敷料研究进展