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DNA barcoding provides distinction between Radix Astragali and its adulterants

science china(Life sciences) 2010年第8期53卷 992-999页

作者： GUO HaiYan1,2, wang WeiWei2, YANG Ning2, GUO BaoLin3, ZHANG Sun2, YANG ruiJing2, yuan Ye2, YU junLin4, HU SongNian2, SUN QiShi1 & YU jun2 1School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical university, Shenyang 110016, china 2Key Laboratory of Genome sciences and Information, beijing Institute of Genomics, Chinese Academy of sciences, beijing 101300, china 3Chinese Academy of Medical sciences, Peking Union Medical college Institute of Medicinal Plant Development, beijing 100094, china 4Department of Chemistry, Tonghua Normal college, Tonghua 134002, china 1. School of Traditional Chinese Materia Medica Shenyang Pharmaceutical University Shenyang 110016 China2. Key Laboratory of Genome Sciences and Information Beijing Institute of Genomics Chinese Academy of Sciences Beijing 101300 China3. Peking Union Medical College Institute of Medicinal Plant Development Chinese Academy of Medical Sciences Beijing 100094 China4. Department of Chemistry Tonghua Normal College Tonghua 134002 China

Based on variable nuclear and/or organellar DNA sequences among vastly divergent species as well as morphologically indistinguishable species, DNA barcoding is widely applicable in species identification, biodiversity studies, forensic analyses, and authentication of medicinal plants. The roots of Astragalus membranaceus and A. membranaceus var. mongholica are commonly used as Radix Astragali in several Asian countries, including china, Japan, and Korea. However, in addition to the two species recorded in the Chinese Pharmacopoeia, there are twenty-three species from different genera including Astragalus, Oxytropis, Hedysarum, and Glycyrrhiza, which have been used as adulterants not only in trading markets but also by the herbal medicine industry. Therefore, a simple, reliable, and accurate classification method is important for distinguishing authentic Radix Astragali from its adulterants. In this study, we acquired data for 37 samples from four related genera within the family Fabaceae. Then we compared four candidate DNA barcoding markers using ITS, matK, rbcL, and coxI sequences from nuclear, chloroplast, and mitochondrial genomes, all commonly used for plants to identify genetic variations among genera, intraspecies, and interspecies. We observed higher divergences among genera and interspecies for ITS, which have the average Kimura 2-parameter distances of 4.5% and 14.1%, respectively, whereas matK was found to have sufficient divergence at the intraspecific level. Moreover, two indels detected in the matK sequence are useful for PCR studies in distinguishing Radix Astragali from its adulterants. This study suggests that the combined barcoding regions of ITS and matK are superior barcodes for Radix Astragali and further studies should focus on evaluating the applicability and accuracy of such combined markers for a wide range of traditional Chinese herbs.

关键词： Radix Astragali DNA barcode Astragalus adulterant identification

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Evaluation of Pathway of Nitrogen Loss in Winter Wheat and Summer Maize Rotation System

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Agricultural sciences in china 2002年第11期1卷 1224-1231页

作者： JU Xiao-tang, LIU Xue-jun, ZOU Guo-yuan, wang Zhao-hui and ZHANG Fu-suo(Department of Plant Nutrition , china Agricultural university , beijing 100094 ,P. R. china) Department of Plant Nutrition China Agricultural University Beijing 100094 P.R. China

The nitrogen loss pathway in winter wheat and summer maize rotation system was studied based on field experimental data. The results showed that nitrogen recovery rate was significantly decreased with nitrogen fertilization rate increased, while residual rate and losses rate had an increasing trend. Accumulated ammonia volatilization loss in winter wheat and summer maize rotation was 12. 8(N0), 22.0(N120), 33. 0(N240) and 64. 5 kg N ha-1 (N360) respectively and rate of ammonia volatilization loss was 3.8, 4.2 and 7.2% respectively while urea was mixed with 0 -10 cm soil or spread before irrigation. Denitrification loss with acetylene-soil core incubation method in winter wheat was lower than 1 kg N ha-1 and rate of denitrification loss was 0. 21 - 0. 26% or trace. Denitrification loss in summer maize was 1 - 14 kg N ha-1 and rate of denitrification loss was 1-5%. The total gaseous loss in winter wheat and summer maize rotation system was less than 10%, and the main nitrogen fertilizer loss way was leaching below 0 - 100 cm soil profile and accumulated in deeper soil.

关键词： Winter wheat Summer maize Ammonia volatilization Denitrif ication Nitrate leaching

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A draft sequence of the rice (Oryza sativa ssp. indica) genome

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Chinese science Bulletin 2001年第23期46卷 1937-1942页

作者： YU jun, HU Songnian, wang jun,LI Songgang WONG Ka-Shu Gane, LIU Bin,DENG Yajun, DAI Li, ZHOU Yan,ZHANG Xiuqing, CAO Mengliang, LIU Jing,SUN Jiandong , TANG Jiabin, CHEN Yanjiong,HUANG Xiaobing, LIN Wei, YE Chen, TONG Wei,CONG Lijuan, GENG Jianing, HAN Yujun, LI Lin,LI Wei, HU Guangqiang, HUANG Xiangang,LI Wenjie, LI Jian, LIU Zhanwei, LI Long,LIU Jianping, Ql Qiuhui, LIU Jinsong, LI Li,wang Xuegang, LU Hong, WU Tingling,ZHU Miao, Nl Peixiang, HAN Hua, DONG Wei,REN Xiaoyu, FENG Xiaoli, GUI Peng,LI Xianran, wang Hao, XU Xin, ZHAI Wenxue,XU Zhao, ZHANG Jinsong, HE Sijie,ZHANG Jianguo, XU Jichen, ZHANG Kunlin,ZHENG Xianwu, DONG Jianhai, ZENG Wanyong,TAO Lin, CHEN Xuewei, HE jun, LIU Daofeng,TIAN Wei, TIAN Chaoguang, XIA Hongai,LI Gang, GAO Hui, LI Ping, CHEN Wei ,wang Xudong, ZHANG Yong, HU Jianfei,wang Jing, LIU Song, YANG Jian,ZHANG Guangyu, XIONG Yuqing, LI Zhijie,MAO Long, ZHOU Chengshu, ZHU Zhen,CHEN Runsheng, HAO Bailin,ZHENG Weimou, CHEN Shouyi, QUO Wei,LI Guojie, LIU Siqi, HUANG Guyang,TAO Ming, wang Jian, ZHU Lihuang,yuan Longping& YANG Huanmingbeijing Genomics Institute/Center of Genomics & Bioinformatics, Chinese Academy of sciences, beijing 101300, china Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang university/Key Laboratory of Bioinformatics of Zhejiang Province, Hangzhou 310007, china Institute of Genetics, Chinese Academy of sciences, beijing 100101, china National Hybrid Rice R & D Center, Changsha 410125, china Laboratory of Bioinformatics, Institute of Biophysics, Chinese Academy of sciences, beijing 100101, china college of Life sciences, Peking university, beijing 100871, china Institute of Theoretical Physics, Chinese Academy of sciences, beijing 1Q0080, china Digital china Ltd., beijing 100080, china Institute of Computing Technology, Chinese Academy of sciences, beijing 100080, china Medical college, Xi’an Jiaotong university, Xi’an 710061, chinaThese authors contributed equally to this work.Corresponding author.Corresponden beijing Genomics Institute/Center of Genomics & Bioinformatics Chinese Academy of Sciences Beijing China Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang university/Key Laboratory of Bioinformatics of Zhejiang Province Hangzhou China college of Life sciences Peking University Beijing China Medical college Xi’an Jiaotong University Xi’an China Institute of Genetics Chinese Academy of Sciences Beijing China Laboratory of Bioinformatics Institute of Biophysics Chinese Academy of Sciences Beijing China National Hybrid Rice R & D Center Changsha China Institute of Theoretical Physics Chinese Academy of Sciences Beijing China Digital china Ltd. Beijing China Institute of Computing Technology Chinese Academy of Sciences Beijing China

The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a "whole genome shotgun" approach, we have pro-duced a draft rice genome sequence of Oryza sativa ssp. in-dica, the major crop rice subspecies in china and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEM-BLER, a custom-designed software package

关键词： rice genome draft sequences.

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High-gravity-assisted green synthesis of rare-earth doped calcium molybdate colloidal nanophosphors

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Chinese Journal of Chemical Engineering 2020年第6期28卷 1744-1751页

作者： yuan Pu Lifeng Lin jun Liu Jiexin wang Dan wang State Key Laboratory of Organic Inorganic Composites Beijing University of Chemical TechnologyBeijing 100029China Research Center of the Ministry of Education for High Gravity Engineering and Technology Beijing University of Chemical TechnologyBeijing 100029China

In this work,we report an innovative route for the synthesis of rare-earth doped calcium molybdate(CaMoO4)nanophosphors by using high gravity rotating packed bed(RPB)technology and paraffin liquid as the *** significant intensified mass transfer and micromixing of reactants in the RPB reactor are benefiting for homogeneous doping of rare-earth ions in the host materials,leading to nanophosphors with high quantum *** use of liquid paraffin as the solvent eliminates the safety risks associated with volatile organic compounds,increasing the potential for clean production of *** excitation of deep ultraviolet(DUV)light,the CaMoO4:Na+,Eu3+nanophosphors exhibit red emission at peak wavelength of 615 nm and quantum yield of up to 35.01%.The CaMoO4:Na+,Tb3+nanophosphors exhibit green emission at peak wavelength of543 nm with quantum yield of up to 30.66%.The morphologies of the nanophosphors are tunable from nanofibers through nanorods to nanodots and the possible mechanism of controlling the formation of different nanostructures is proposed on the basis of experimental results and theoretical analysis of *** nanophosphors are highly dispersible in organic solvents and utilized for fabricating fabrication of flexible,freestanding luminescent films based on silicone *** also demonstrate the red LEDs consisting of the hybrid films of CaMoO4:Na+,Eu3+nanoparticles as color-converting phosphors and DUV LEDs as illuminators,offering strong potential for future nanophosphors-basedsolid-state lighting systems.

关键词： Colloidal nanophosphors CaMoO4 Process intensification High gravity technology Green solvent

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Investigating the Nucleation Effect of DMDBS on Syndiotactic Polypropylene from the Perspective of Chain Conformation

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Chinese Journal of Polymer science 2020年第12期38卷 1355-1364页

作者： Xin-Xin wang Jian-jun Yi Li wang yuan yuan Jia Chun Feng State Key Laboratory of Molecular Engineering of Polymers Department of Macromolecular ScienceFudan UniversityShanghai 200433China Laboratory for Synthetic Resin Research Petrochina Petrochemical Research Institute Beijing 102200China

The mechanism of nucleating agents(NAs)accelerating the crystallization of semi-crystlline polymers has received continuous attention due to the extreme importance in academic research and industry *** this work,the nucleation effect and probable mechanism of 1,3.2,4 bis(3,4-dimethylbenzylidene)sorbitol(DMDBS)on promoting the crystallization of syndiotactic polypropylene(sPP)was systematically *** results showed that DMDBS could significantly accelerate the crystallization process and did not change the crystalline form of *** in situ infrred spectra recorded in the crystallization process showed that in pristine sPP the tttt conformers decreased and the tgg conformers increased *** sPP/DMDBS system,DMDBS could promote the increase of tgg conformers rather than the decrease of tttt *** further analysis by 2D-IR spectra revealed that ttgg conformers increased prior to the decrease of tttt conformers in the sPP/DMDBS system comparing with pristine *** that ttgg conformers were basic elements of helical conformation of Form I crystal for sPP,we proposed a probable nucleation mechanism of DMDBS for sPP:DMDBS could stabilize the ttgg conformers which induced these ttgg conformers to pre-orientate and aggregate into helical conformation sequences as initial nuclei quickly and early to promote the sPP *** work provides some new insights into the nucleation mechanism of NAs for sPP.

关键词： Syndiotactic polypropylene Nucleating agent Crsallization Conformation

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Research on Structure Dynamic Neural Network

Research on Structure Dynamic Neural Network

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2008建模、辨识与控制国际会议

作者： QIAO jun-fei college of Electronic and Control Engineering, beijing university of Technology, beijing, china. HAN Hong-gui college of Electronic and Control Engineering, beijing university of Technology, beijing, china. LI Xin-yuan college of Electronic and Control Engineering, beijing university of Technology, beijing, china.

A model of structure dynamic neural network, which is simulates the learning skills such as human beings and animals, is presented. This model contains two main steps: 1) The structure learning phase possesses the ability of online generation and ensures the number of the neural nodes of the neural network;2) The parameter learning phase adjusts the interconnection weights of neural network to achieve favourable approximation performance. The structure learning algorithm consists of growing and pruning methods, and then the Lyapunov stability theory is used to analyze the stability of this algorithm. Finally, we use this new neural network to track the nonlinear functions, simulation results show that this new algorithm can achieve favourable performance.

关键词： neural network structure structure dynamic task scheduling growing and pruning methods.

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Direct extraction of topological Zak phase with the synthetic dimension

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Light(science & Applications) 2023年第4期12卷 671-679页

作者： Guangzhen Li Luojia wang rui Ye yuanlin Zheng Da-Wei wang Xiong-jun Liu Avik Dutt Luqi yuan Xianfeng Chen State Key Laboratory of Advanced Optical Communication Systems and Networks School of Physics and AstronomyShanghai Jiao Tong UniversityShanghai 200240China Shanghai Research Center for Quantum sciences Shanghai 201315China Interdisciplinary Center for Quantum Information and Zhejiang Province Key Laboratory of Quantum Technology and Device Department of PhysicsZhejiang UniversityHangzhou 310027China InternationalCenter for Quantum Materials and School of Physics Peking UniversityBeijing 100871China International Quantum Academy Shenzhen 518048China Department of Mechanical Engineering Institute for Physical Science and TechnologyUniversity of MarylandCollege ParkMD 20742USA Collaborative Innovation Center of Light Manipulation and Applications Shandong Normal UniversityJinan 250358China

Measuring topological invariants is an essential task in characterizing topological phases of *** are usually obtained from the number of edge states due to the bulk-edge correspondence or from interference since they are integrals of the geometric phases in the energy *** is commonly believed that the bulk band structures could not be directly used to obtain the topological ***,we implement the experimental extraction of Zak phase from the bulk band structures of a Su-Schrieffer-Heeger(SSH)model in the synthetic frequency *** synthetic SSH lattices are constructed in the frequency axis of light,by controlling the coupling strengths between the symmetric and antisymmetric supermodes of two bichromatically driven *** measure the transmission spectra and obtain the projection of the time-resolved band structure on lattice sites,where a strong contrast between the non-trivial and trivial topological phases is *** topological Zak phase is naturally encoded in the bulk band structures of the synthetic SSH lattices,which can hence be experimentally extracted from the transmission spectra in a fiber-based modulated ring platform using a laser with telecom *** method of extracting topological phases from the bulk band structure can be further extended to characterize topological invariants in higher dimensions,while the exhibited trivial and non-trivial transmission spectra from the topological transition may find future applications in optical communications.

关键词： topological trivial synthetic

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一种运动学形式的人员疏散仿真微观模型研究

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系统仿真学报 2008年第21期20卷 5791-5794页

作者：黄希发王科俊郭莲英李祥晨肖书明哈尔滨工程大学自动化学院哈尔滨150001 国家体育总局体育科学研究所北京100061 北京航空航天大学自动化科学与电气工程学院北京100083

从疏散群体中的人员个体角度出发研究人员疏散规律,提出一种运动学形式的离散时间仿真微观模型。每个个体的运动完全由其运动方向及速度大小所确定,将个体的运动姿态用两个笛卡儿坐标和一个方向角表示,进行运动学近似,个体运动过程由速... 详细信息

从疏散群体中的人员个体角度出发研究人员疏散规律,提出一种运动学形式的离散时间仿真微观模型。每个个体的运动完全由其运动方向及速度大小所确定,将个体的运动姿态用两个笛卡儿坐标和一个方向角表示,进行运动学近似,个体运动过程由速度的两个"瞬时"值描述,即平移线速度和偏转角速度。进而考虑影响个体运动方向及速度大小的主要行为原则,建立综合这些行为影响因素的模型方程,允许通过调节各种参数,研究环境及个体心理状态对疏散的影响。

关键词：人员疏散离散时间仿真运动学微观模型恐慌

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Structure,Binding Characteristics,and 3D Model Prediction of a Newly Identified Odorant-Binding Protein from the Cotton Bollworm,Helicoverpa armigera (Hübner)

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Journal of Integrative Agriculture 2012年第3期11卷 430-438页

作者： ZHANG Tian-tao wang Wei-xuan GU Shao-hua ZHANG Zi-ding WU Kong-ming ZHANG Yong-jun GUO Yu-yuan Northwest Agriculture and Forestry university Yangling 712100 P.R. China State Key Laboratory of Plant Diseases and lnsect Pests/Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 P.R. China State Key Laboratory of Agrobiotechnology/college of Biological sciences China Agricultural University Beijing 100193 P.R. China

The full-length sequence of the odorant binding protein 5 gene,HarmOBP5,was obtained from an antennae cDNA library of cotton bollworm,Helicoverpa armigera （Hübner）.The cDNA contains a 444 bp open reading frame,encoding a protein with 147 amino acids,namely ***5 was expressed in Escherichia coli and the recombinant protein was purified by affinity ***-PAGE and Western blot analysis demonstrated that the purified protein can be used for further investigation of its binding *** binding assays with 113 odorant chemicals indicated that HarmOBP5 has strong affinity to some special plant volatiles,including （E）-β-farnesene,ethyl butyrate,ethyl heptanoate,and acetic acid 2-methylbutyl *** on three-dimensional （3D） model of AaegOBP1 from Aedes aegypti,a 3D model of HarmOBP5 was *** model revealed that some key binding residues in HarmOBP5 may play important roles in odorant perception of *** study provides clues for better understanding physiological functions of OBPs in *** and other insects.

关键词： odorant binding protein fluorescence binding assay 3D model Helicoverpa armigera (Hübner)

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Report of 16 kindreds and one kindred with hMLH1 germline mutation

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World Journal of Gastroenterology 2002年第2期8卷 263-264页

作者： Bo Zhao Zhen-jun wang yuan-Lian Wan Peng Li Yan-Ting Huang,Department of Surgery,Peking university First Hospital,beijing 1000.34,china Yu-Feng Xu,Experimental Center,Peking university First Hospital,beijing 100034,china Department of Surgery Peking University First Hospital No. 8 Xishiku Street Western Distract Beijing 100034 China.

AIM: To analyze the diagnosis and treatment of 16 hereditarynonpolyposis colorectal cancer (HNPCC) kindreds, and toreport the first kindred with hMLH1 germline mutation inchina's ***: The diagnosis, treatment and follow-up study of16 HNPCC kindreds were retrospectively reviewed. Dataconceming site of the malignant tumor, age st the diagnosis,history of synchronous and/ or metachronous cancer, andhistopathology of tumors were recorded. All treatments hadwon formal consent. PCR and SSCP were used to screen thecoding region of hMLH1 and hMSH2 genes. Variant bandswere sequenced by a 377 DNA ***: Among sixteen kindreds, sixty-eight patients hada mean age of 50.8 years, including twenty-one multiplecancer patients and forty-six colorectal cancer patients(metachronous colorectal cancers in sixteen). A total of onehundred and one malignant neoplasms were found in thesesixty-eight patients, including 50 colonic, 17 rectal, 11gastric, 7 endometrial, and 4 esophageal cancers. 39.5%colorectal patients had metachronous cancers within tenyears who needed reopeertions. A germline G265T nonsensemutation was found in the third exon of hMLH1, resulting in astop codon and truncated protein. Three phenotypically normalfamily members were also found to carry the mutated ***: HNPCC is a typical auto-dominant hereditaryisease, the main characteristics include early onset andfrequency of cancers; predominance of colorectal,especially right-sided colon cancers; frequency of multipleprimary cancers (especially colorectal cancers). Segmentalresection for colorectal cancers is not eligible for colorectalcancer patient in HNPCC kindreds. Intensive follow-up isessential for all patients and possible gene carriers. The firstHNPCC kindred with hMUH1 gene germline mutation wasidentified in china's Mainland, and three phenotypicallynormal family members were found to be carriers of themutated gene. The G265T germline (nonsense) mutation inthe third exon of hMLH1

关键词：亲缘族 hMLH1 种类突变结直肠癌

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