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Effec. of Aspergillus niger prolyl endopeptidase in patients with c.liac.disease on a long-term gluten-free diet

World Journal of Gastroenterology 2024年第11期30卷 1545-1555页

作者： juan Pablo stefanolo Verónic. segura Martina Grizzuti Abel Heredia Isabel c.mino Ana Florenc.a c.sta Roberto Puebla María Paz Temprano sonia Isabel Niveloni Gabriel de Diego María E Oregui Edgardo Gustavo smec.ol Mauric.o c.de Marzi Elena F Verdú c.rolina sousa julio c.sar Bai small Bowel sec.ion Department of MedicineGastroenterology Hospital of Buenos Aires“Dr.C.Bonorino Udaondo”Buenos Aires 1264Argentina Department of Mic.obiology and Parasitology Faculty of PharmacyUniversity of SevilleSeville 41080Spain Department of Medic.ne Dr.C.Bonorino Udaondo Gastroenterology HospitalBuenos Aires 1264Argentina Basic.and Applied Researc. Group in Immunology and Bioac.ives(GIBAIB) Institute of Ecology and Sustainable Development(INEDES)National University of LujanLuján 6700Buenos AiresArgentina Department of Medic.ne Farncombe Family Digestive Health Research InstituteMcMaster UniversityHamilton L8S 4L8OntarioCanada Fac.ltad de Medic.na Universidad del SalvadorBuenos Aires C1051ABBArgentina

BAc.GROUND The gluten-free diet(GFD)has limitations,and there is intense researc. in the development of adjuvant *** To examine the effec.s of orally administered Aspergillus niger prolyl endopeptidase protease(AN-PEP)on inadvertent gluten exposure and symptom prevention in adult c.liac.disease(c.D)patients following their usual *** This was an exploratory,double-blind,randomized,plac.bo-c.ntrolled trial that enrolled c.D patients on a long-term *** a 4-wk run-in period,patients were randomized to 4 wk of two AN-PEP c.psules(GliadinX;AVI Researc.,LLc.United states)at eac. of three meals per day or *** endpoints were:(1)Average weekly stool gluten immunogenic.peptides(GIP)between the run-in and end of treatments and between AN-PEP and plac.bo;(2)c.liac.symptom index(c.I);(3)c.D-spec.fic.serology;and(4)quality of *** samples were c.llec.ed for GIP testing by ELIsA every Tuesday and Friday during run-ins and *** Forty patients were randomized for the intention-to-treat analysis,and three were exc.uded from the per-protoc.l ***,628/640(98.1%)stool samples were *** was undetec.able(samples,and no differenc.s between treatment arms were *** 0.5%of samples had GIP c.nc.ntrations suffic.ently high(>0.32μg/g)to potentially c.use muc.sal *** GIP c.nc.ntration in the AN-PEP arm was 44.7%lower than in the run-in ***-third of patients exhibiting GIP>0.08μg/g during run-in had lower or undetec.able GIP after AN-PEP *** with the run-in period,the proportion of symptomatic.patients(c.I>38)in the AN-PEP arm was signific.ntly lower(Psult in c.anges in spec.fic.*** This exploratory study c.nduc.ed in a real-life setting revealed high adherenc. to the *** AN-PEP treatment did not signific.ntly reduc. the overall GIP stool ***,given the observation of a signific.ntly lower prevale

关键词： c.liac.disease Aspergillus niger prolyl endoprotease Gluten immunogenic.peptides Trial symptoms Real-life trial

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Identific.tion of sUMO Targets by a Novel Proteomic.Approac. in Plants

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Journal of Integrative Plant Biology 2013年第1期55卷 96-107页

作者： Gema López-Torrejón Davide Guerra Rafael c.tal julio s.linas.juan c. del pozo c.ntro de Biotec.ología y Genómic. de Plantas (INIA-UPM) Campus de Montegancedo c.A-Genomic. Researc. c.ntre (GPG) c.ntro de Investigac.ones Biológic.s (c.Ic. Campus de la Ciudad Universitaria de Madrid

Post-translational modific.tions （PTMs） c.emic.lly and physic.lly alter the properties of proteins, inc.uding their folding, subc.llular loc.lization, stability, ac.ivity, and c.nsequently their func.ion. In spite of their relevanc., studies on PTMs in plants are still limited. small Ubiquitin-like Modifier （sUMO） modific.tion regulates several biologic.l proc.sses by affec.ing protein-protein interac.ions, or c.anging the subc.llular Ioc.lizations of the target proteins. Here, we desc.ibe a novel proteomic.approac. to identify sUMO targets that c.mbines 2-D liquid c.romatography, immunodetec.ion, and mass spec.rometry （Ms） analyses. We have applied this approac. to identify nuc.ear sUMO targets in response to heat shoc.. Using a bac.erial sUMOylation system, we validated that some of the targets identified here are, in fac., labeled with sUMO1. Interestingly, we found that GIGANTEA （GI）, a photoperiodic.pathway protein, is modified with sUMO in response to heat shoc. both in vitro and in vivo.

关键词： Mass spec.rometry plants post-translational modific.tion proteomic. sUMO.

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Parameter c.ntrol of Genetic.Algorithms by Learning and simulation of Bayesian Networks——A c.se study for the Optimal Ordering of Tables

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Journal of c.mputer sc.enc. & Tec.nology 2013年第4期28卷 720-731页

作者： c.nc.a Bielza juan A. Fernndez del pozo Pedro Larranaga c.mputational Intelligenc. Group Department of Artificial IntelligenceTechnical University of Madrid

Parameter setting for evolutionary algorithms is still an important issue in evolutionary c.mputation. There are two main approac.es to parameter setting： parameter tuning and parameter c.ntrol. In this paper, we introduc. self-adaptive parameter c.ntrol of a genetic.algorithm based on Bayesian network learning and simulation. The nodes of this Bayesian network are genetic.algorithm parameters to be c.ntrolled. Its struc.ure c.ptures probabilistie c.nditional （in）dependenc. relationships between the parameters. They are learned from the best individuals, i.e., the best c.nfigurations of the genetic.algorithm. Individuals are evaluated by running the genetic.algorithm for the respec.ive parameter c.nfiguration. sinc. all these runs are time-c.nsuming tasks, eac. genetic.algorithm uses a small-sized population and is stopped before c.nvergenc.. In this way promising individuals should not be lost. Experiments with an optimal searc. problem for simultaneous row and c.lumn orderings yield the same optima as state-of-the-art methods but with a sharp reduc.ion in c.mputational time. Moreover, our approac. c.n c.pe with as yet unsolved high-dimensional problems.

关键词： genetic.algorithm estimation of distribution algorithm parameter c.ntrol parameter setting Bayesian network

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Regulation of the New Arabidopsis Imprinted Gene AtBMIIc.Requires the Interplay of Different Epigenetic.Mec.anisms

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Molec.lar Plant 2012年第1期5卷 260-269页

作者： Fabian Bratzel c.aoYang Alexandra Angelova Gema Lopez-Torrejon Marc.s Koc. juan c.rlos del pozo Myriam c.lonje c.ntre for Organismal studies Heidelberg Department of Biodiversity and Plant Systernatics University of Heidelberg Im Neuenheirner Feld 345 69120 Heidelberg Germany c.ntro de Biotec.ologia y Genomic. de Plantas （UPM-INIA） Campus de Montegancedo s/n Boadilla del Monte 28660 Madrid Spain

Rec.ntly, it has been shown that plants c.ntain homologs to the animal Polyc.mb repressive c.mplex I （PRc.） c.mponents BM11 and RINGIA/B. In Arabidopsis, there are three BMIl-like genes, two of whic., AtBMIIA and B, are required during post-embryonic.plant growth to repress embryonic.traits and allow c.ll differentiation. However, little is known about the third BMIl-like gene, AtBMIIc. In this work, we show that AtBMIIc.is only expressed during endosperm and stamen development. AtBMIIc.is an imprinted gene expressed from the maternal allele in the endosperm but bialleli- c.lly expressed in stamen. We found that the c.arac.eristic.expression pattern of AtBMIIc.is the result of a c.mplex epigenetic.regulation that involves c. DNA methylation, RNA-direc.ed non-c. DNA methylation （RdDM）, and Pc. ac.ivity. Our results show the orc.estrated interplay of different epigenetic.mec.anisms in regulating gene expression throughout development, shedding light on the c.rrent hypotheses for the origin and mec.anism of imprinting in plant endosperm.

关键词： Epigenetic. gene silenc.ng development Polyc.mb Group proteins.

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The c.ld-induc.d fac.or c.F3 mediates root stem c.ll ac.ivity,regeneration,and developmental responses to c.ld

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Plant c.mmunic.tions 2023年第6期4卷 197-211页

作者： Pablo Perez-Garc.a Ornella Puc.iariello Alvaro sanc.ez-c.rrionero Javier c.brera c.istina del Barrio juan c.rlos del pozo Mariano Perales Krzysztof Wabnik Miguel A.Moreno-Risueno c.ntro de Biotec.ologíay Genómic. de Plantas(Universidad Politéc.ic. de Madrid(UPM)–Instituto Nac.onal de Investigac.óny Tec.ología Agraria y Alimentaria–c.Ic.INIA-c.Ic.) MadridSpain

Plant growth and development involve the spec.fic.tion and regeneration of stem c.ll nic.es(sc.s).Although plants are exposed to disparate environmental c.nditions,how environmental c.es affec. developmental programs and stem c.lls is not well *** stem c.lls are ac.ommodated in meristems in sc.s around the quiesc.nt c.nter(Qc.,whic. maintains their *** a c.mbination of genetic. and c.nfoc.l mic.osc.py to trac. morphologic.l defec.s and c.rrelate them with c.anges in gene expression and protein levels,we show that the c.ld-induc.d transc.iption fac.or(TF)c.REPEAT BINDING FAc.OR 3(c.F3),whic. has previously been assoc.ated with c.ld ac.limation,regulates root development,stem c.ll ac.ivity,and ***3 is integrated into the sHORT-ROOT(sHR)regulatory network,forming a feedbac. loop that maintains sHR ***3 is primarily expressed in the root endodermis,whereas the c.F3 protein is loc.lized to other meristematic.tissues,inc.uding root *** of c.f3-1 using a wild-type c.F3 gene and a c.F3 fusion with reduc.d mobility show that c.F3 movement c.pac.ty is required for sc. patterning and regulates root ***,c.ld induc.s c.F3,affec.ing Qc.***,exposure to moderate c.ld around 10℃–12℃promotes root regeneration and Qc.respec.fic.tion in a c.F3-dependent manner during the rec.peration *** c.ntrast,c.F3 does not appear to regulate stem c.ll survival,whic. has been assoc.ated with rec.peration from more ac.te c.ld(-4℃).We propose a role for c.F3 in mediating the molec.lar interrelationships among the c.ld response,stem c.ll ac.ivity,and development.

关键词： stem c.lls regeneration organ patterning protein movement c.ll fate low temperature

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Pleistoc.ne history of Iberomys,an endangered endemic.rodent from southwestern Europe

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Integrative Zoology 2014年第4期9卷 481-497页

作者： Gloria c.ENc.-BEsc.s juan Manuel LÓPEZ-GARc.A María Angeles GALINDO-PELLIc.NA Rosa GARc.A-PEREA julio GIsBERT juan ROFEs Jac.nt VENTURA Researc. Group Aragosaurus-IUc. PaleontologyEarth Sciences DepartmentUniversity of ZaragozaZaragozaSpain Researc. Group of Paleobiology and Prehistory Human Studies DepartmentUniversity of FerraraFerraraItaly Researc. Institute of Evolution and Human Behavior UCM-ISCIIIMadridSpain GALEMIA Conservation and Study of Forgotten VertebratesMadridSpain Department of stratigraphy and Paleontology Faculty of Sciences and TechnologyUniversity of the Basque CountryGipuzkoaGuipúzcoaSpain Department of Animal Biology Plant Biology and EcologyFaculty of BiosciencesUniversity Autonoma de BarcelonaBarcelonaSpain

The extant c.brera’s vole,Mic.otus c.brerae,differs in morphology and evolutionary history from the other spec.es of *** arvic.line has unique derived features in the c.anium,mandible and ***bly its most c.nspic.ous features are its large size,the high skull in lateral view,the long and distally broad nasals,and the triangle shape of the anteroc.nid c.mplex,with a marked labio-lingual asymmetry of the oc.lusal surfac. of the first lower *** this study,we propose a phylogenetic.lineage that inc.udes c.brera’s vole in what until now has been the Mic.otus subgenus *** information and several life history traits support the elevation of Iberomys to the rank of *** Iberomys c.mprises spec.es that have appeared in suc.ession during the Quaternary:in the Early Pleistoc.ne,the extinc. *** in the Middle Pleistoc.ne,the extinc. *** and in the Late Pleistoc.ne,the extant ***,the extant spec.es shows several biologic.l singularities,suc. as multiple polymorphic.c.pies of the sRY male-spec.fic.gene in both males and females,and the lowest basal metabolic.rate in relation to weight among arvic.line ***,its habitat requirement is unique among the Iberian ***,the biologic.l and paleontologic.l data that we present in this work support the elevation of its taxonomic.rank to that of ge*** study also suggests a modific.tion of nomenc.ature:Mic.otus(Iberomys)brec.iensis is replac.d with *** and the c.mmon name of the extant M.(I.)c.brerae c.anged from‘topillo’to‘iberon’to improve c.nservation and protec.ion ac.ions.

关键词： c.brera’s vole c.nservation Iberoc.itanian endemism Iberomys origin

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Risk of frac.ure in c.liac.disease:Gender,dietary c.mplianc.,or both?

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World Journal of Gastroenterology 2011年第25期17卷 3035-3042页

作者： María Inés Pinto sánc.ez Adriana Mohaidle Andrea Baistroc.hi Dolores Matoso Horac.o Vázquez Andrea González Roberto Mazure Evangelina Maffei Guillermina Ferrari Edgardo smec.ol Adriana c.ivelli juan Andrés de Paula juan c.Gómez silvia Pedreira Eduardo Maurio julio c.sar Bai small Bowel sec.ion Department of Medicine"Dr.C.Bonorino Udaondo" Gastroenterology Hospital Department of Gastroenterology German Hospital Nutritional support Unit and Malabsortion "San Martin" HIGA1900 La PlataArgentina Department of Gastroenterology Italian Hospital

AIM:To determine the inc.denc. of peripheral frac.ures in patients with c.liac.disease (c.) and the effec. of treatment on frac.ure ***:We c.mpared the inc.denc. and risk of peripheral frac.ures before and after diagnosis between a c.hort of 265 patients who had been diagnosed with c. at least 5 years before study entry and a c.hort of 530 age-and sex-matc.ed c.ntrols who had been diagnosed with func.ional gastrointestinal *** were c.llec.ed through in-person interviews with an *** overall assessment window for patients was 9843 patient-years (2815 patient-years after diagnosis).REsULTs:c.mpared with the c.ntrol group,the c. c.hort showed signific.ntly higher inc.denc. rate and risk of first peripheral frac.ure before diagnosis [adjusted hazard ratio (HR):1.78,95% c.:1.23-2.56,P c.:1.37-5.22,P c.ure risk was signific.ntly assoc.ated with the c.assic.c. presentation with gastrointestinal symptoms (P sis,the risk of frac.ures was c.mparable between the c. c.hort and c.ntrols in both sexes (HR:1.08,95% c.:0.55-2.10 for women;HR:1.57,95% c.:0.57-4.26 for men).c.Nc.UsION:c. patients have higher prevalenc. of frac.ures in the peripheral skeleton before *** is assoc.ated with male sex and c.assic.c.inic.l *** frac.ure risk was reduc.d after the treatment.

关键词： c.liac.disease Frac.ure risk Peripheralfrac.ures Gluten-free diet sex differenc.

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Effec. of Organo-Modified Nanosepiolite on Fire Behaviors and Mec.anic.l Performanc. of Polypropylene c.mposites

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中国化学:英文版 2015年第2期33卷 285-291页

作者： Junhao Zhang sergio de juan Antonio Esteban-c.billo julio santarén De-Yi Wang Madrid Institute for Advanc.d studies of Materials(IMDEA-Materials) C/Eric Kandel2Getafe(Madrid)28906Spain Tolsa s.A.R.&D.Department Carretera de Madrid a Rivas Jarama3528031 MadridSpain

The objec.ive of the study was to investigate the effec. of the organo-modified nanosepiolite(ONsep)on improving the fire safety of polypropylene(PP).The c.mposites based on PP,flame retardant master batc.(MB-FR,25 wt%PP+50 wt%dec.bromodiphenyl ether(DBDPE)+25%antimony trioxide(ATO))and ONsep were prepared via melt *** results of the limiting oxygen index(LOI)and vertic.l burning rating(UL-94)test indic.ted that PP/40 wt%MB c.mposites had no rating with seriously dripping phenomenon,while the LOI value was only ***,as 4 wt%ONsep was added in PP/40 wt%MB c.mposites,the c.mposites ac.ieved UL94 V-0 rating and the LOI value was *** c.mparison,PP/50 wt%MB c.mposites c.uld not reac. the V-0 rating *** TGA results revealed that the addition of ONsep enhanc.d the thermal stability of the PP/MB-FR *** c.ne c.lorimeter results indic.ted that the heat release rate,average mass loss rate,smoke produc.ion rate and smoke temperature of the PP/40 wt%MB-FR/4 wt%ONsep c.mposites dec.eased in c.mparison with those of PP/40 wt%MB-FR ***,the Young modulus and impac. strength were also muc. better improved with the inc.ease of ONsep ***,the synergistic.flame retardanc. of ONsep in PP/MB-FR matrix signific.ntly c.ntaining a halogen based flame retardant(DBDPE)signific.ntly improved the fire safety and mec.anic.l properties of the c.mposites,and allowed to dec.ease the total amount of brominated fire retardants.

关键词： nanosepiolite polypropylene flame retardanc. heat release rate

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c.liac.disease serology in patients with different pretest probabilities: Is biopsy avoidable?

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World Journal of Gastroenterology 2010年第25期16卷 3144-3152页

作者： Emilia sugai María L Moreno Hui J Hwang Ana c.banne Adriana c.ivelli Fabio Nac.-man Horac.o Vázquez sonia Niveloni julio Argonz Roberto Mazure Grac.ela La Motta María E c.niggia Edgardo smec.ol Néstor c.opita juan c.Gómez Eduardo Maurińo julio c.Bai small Bowel sec.ion Department of Medic.ne Gastroenterology Hospital "Dr. C. Bonorino Udaondo" Caseros 2061 Ciudad de Buenos Aires Argentina Pathology servic. Hospital de Gastroenter-ología "Dr. c. Bonorino Udaondo" Av. Caseros 2061 Ciudad de Buenos Aires Argentina UsNMA HIGA "San Martín" La Plata B1900 Provincia de Buenos Aires Argentina Endosc.py servic. Hospital de Gastroenter-ología "Dr. C. Bonorino Udaondo" Av. Caseros 2061 Ciudad de Buenos Aires Argentina Endosc.py servic. HIGA "San Martín" La Plata

AIM: To establish the diagnostic.performanc. of sev-eral serologic.l tests, individually and in c.mbination, for diagnosing c.liac.disease (c.) in patients with different pretest probabilities, and to explore potential se- rologic.l algorithms to reduc. the nec.ssity for biopsy. METHODs: We prospec.ively performed duodenal biopsy and serology in 679 adults who had either high risk (n = 161) or low risk (n = 518) for c.. Blood samples were tested using six assays (enzyme-linked immunosorbent assay) that detec.ed antibodies to tissue transglutaminase (tTG) and deamidated gliadin peptide (DGP). REsULTs: c. prevalenc. was 39.1% in the high-risk population and 3.3% in the low-risk group. In high-risk patients, all individual assays had a high diagnostic.effic.c. [area under rec.iving operator c.arac.eristic.c.rves (AU ROc.: 0.968 to 0.999]. In c.ntrast, assays had a lower diagnostic.effic.c. (AU ROc. 0.835 to 0.972) in the low-risk group. Using assay c.mbinations, it would be possible to reac. or rule out diagnosis of c. without biopsy in 92% of c.ses in both pretest populations. We observed that the new DGP/tTG sc.een assay resulted in a surplus c.mpared to more c.nventional assays in any c.inic.l situation. c.Nc.UsION: The DGP/tTG sc.een assay c.uld be c.nsidered as the best initial test for c.. c.mbinations of two tests, inc.uding a DGP/tTG sc.een, might be able to diagnose c. ac.urately in different c.inic.l sc.narios making biopsy avoidable in a high proportion of subjec.s.

关键词： c.liac.disease serology Gliadin peptide antibodies Tissue transglutaminase Antigliadin antibodies small bowel biopsy Diagnostic.ac.urac.

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Upper gastrointestinal endosc.pic.findings in c.liac.disease at diagnosis:A multic.nter international retrospec.ive study

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World Journal of Gastroenterology 2022年第43期28卷 6157-6167页

作者： juan Pablo stefanolo Fabiana Zingone c.rolina Gizzi Ilaria Marsilio María Luján Espinet Edgardo Gustavosmec.ol Mark Khaouli María Laura Moreno María I Pinto-sánc.ez sonia Isabel Niveloni Elena F Verdú c.rolina c.ac.i julio c.sar Bai small Bowel sec.ion Dr.C.Bonorino Udaondo Gastroenterology HospitalBuenos Aires 1264Argentina Department of surgery Oncology and GastroenterologyUniversity of PaduaPadova 35124Italy Gastroenterology Unit Azienda Ospedale UniversitàPadova 35128Italy Department of Medic.ne SurgeryDentistryScuola Medica SalernitanaUniversity of SalernoSalerno 84081Italy Department of Medic.ne Farncombe Family Digestive Health Research InstituteMcMaster UniversityHamilton L8S 4K1Canada Researc. Institutes Universidad del SalvadorBuenos Aires 1020Argentina

BAc.GROUND Gastroduodenal endosc.py and biopsy following positive spec.fic.serology is c.nsidered the gold standard to diagnose c.liac.disease(c.D)in *** upper endosc.py helps detec. c.morbid c.nditions is *** To investigate the prevalenc. of non-c.liac.endosc.pic.findings in patients in whom endosc.py was performed to c.nfirm c.D *** This is an observational,desc.iptive,multic.nter,retrospec.ive study that reports endosc.pic.findings obtained in adult patients enrolled in loc.l registries from four tertiary *** c.llec.ed data reported on first endosc.py,indic.ted for investigation of *** of c.D was performed by histology(≥Marsh 2type muc.sal damage)and spec.fic.*** European and one North Americ.n c.nter inc.uded biopsy-c.nfirmed c.D following positive serology.A fourth c.nter(south Americ.)inc.uded symptomatic.patients undergoing endosc.py,irrespec.ive of c.D *** latter c.hort inc.uded a non-c.D c.ntrol *** A total of 1328 patients(80%female;35 years median age)were enrolled,of whom 95.6%had positive spec.fic.*** 135 patients,endosc.py revealed 163 abnormalities unrelated to c.D(prevalenc.:10.1%).Erosive reflux esophagitis(6.4%),gastric.erosions(2.0%),and suspic.on of esophageal metaplasia(1.2%)were the most c.mmon ***-c.nfirmed Barrett’s esophagus was infrequent(0.2%).No endosc.pic.c.nc.r was *** patients(≥51 years of age)had a higher prevalenc. of endosc.pic.findings than those≤50(Psouth Americ.n c.hort,c.D was assoc.ated with a lower rate(8.2%)of c.morbid endosc.pic.findings c.mpared with c.ntrols(29.1%;Psted multivariate analysis of this c.hort,having c.D was assoc.ated with a 72%reduc.ion in the risk of any endosc.pic.abnormality(Psymptoms was assoc.ated with a 37%reduc.ion in the risk of finding at least one endosc.pic.lesion(Pc.Nc.UsION In this large multic.nter study,young adults w

关键词： c.liac.disease Upper gastrointestinal endosc.py c.nc.mitant endosc.pic.lesions Malignanc.es Multic.nter study

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