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MALDI-TOF MS Assessment to Identify Environmental Mycobacteria

Advances in Microbiology 2015年第9期5卷 620-629页

作者： Camilla Pereira de Paula Uzam Urze Adomaitis Brianesi Camila Romagnoli Karen Machado Gomes Rafael Silva Duarte Erica Chimara julio cezar franco de oliveira Marcelo Affonso Vallim Renata Castiglioni Pascon Cristina Viana-Niero Instituto de Ciencias Ambientais Químicas e Farmaceuticas da Universidade Federal de Sao Paulo Diadema Brasil Instituto de Ciências Ambientais Químicas e Farmacêuticas da Universidade Federal de Sao Paulo Diadema Brasil Instituto de Microbiologia Paulo de Góes da Universidade Federal do Rio de Janeiro Rio de Janeiro Brasil Núcleo de Tuberculose e Micobacterioses do Instituto Adolfo Lutz Sao Paulo Brasil

Over the past few decades, there has been a significant increase in the number of mycobacterial species described. Currently, the genus?Mycobacterium?consists of 170 species. Most species are called nontuberculous mycobacteria (NTM) and are potentially or rarely pathogenic and ubiquitous. One of the main challenges in mycobacteriology is the rapid and precise identification of these microorganisms. In this work, we compared two protein extraction protocols for the identification of 38 reference strains and clinical isolates, representing 27 species, by mass spectrometry (MALDI-TOF MS) to subsequently use the best method for identifying environmental mycobacteria. The results obtained with reference strains and clinical isolates showed that protocol A was effective in identifying 92.1% of mycobacterial specimens at the species level and protocol B, 50%. Therefore, protocol A was evaluated for the rapid identification of 27 environmental mycobacterial isolates. These isolates were subjected to PCR-restriction enzyme analysis (PRA-hsp65). Two isolates were misidentified by PRA-hsp65, whereas MALDI-TOF MS was able to identify them correctly. The results were confirmed by?hsp65 and 16S rRNA gene sequencing. Mass spectrometry has the advantage of being a simpler and faster technique than PRA-hsp65, and our results showed that MALDI-TOF MS is a valuable tool for the identification of environmental mycobacterial isolates.

关键词： MALDI-TOF MS Environmental Mycobacteria Identification PRA-Hsp65

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Increasing analyzable steel thickness for thin layer activation experiments

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Nuclear Science and Techniques 2020年第3期31卷 35-44页

作者： Joao Francisco de oliveira Antunes Mauro Lucio Borges Lemos Michel de Almeida Franca julio cezar Suita Celso Marcelo Franklin Lapa Instituto de Engenharia Nuclear Rio de Janeiro 21941-614Brazil Programa de Engenharia Nuclear-COPPE/UFRJ Riode Janeiro 21941-450Brazil

The thin layer activation technique(TLA)is very effective in wear analysis for tribological systems where disassembly is costly or *** its development in the 1970s,TLA has been applied through direct irradiation of solid targets using ionizing particle beams to obtain an activation with a specific *** profile is then used to measure wear in a variety of *** experiments rely heavily on how deep the normalized activation *** of 30μm to 50μm are obtained for constant activation by the standard method,which for many experiments is ***,many others would benefit greatly from a 300μm constant activation layer,such as high-performance engines,softer alloys(brakes),and oil transport *** this aim was the goal of this ***,we needed to find an adequate irradiation line for the TLA ***,another technique/system was developed(the Attenuation Wheel),which would bring the depth of constant activation from 50μm to 300μm.

关键词： Thin layer activation Wear Proton beam Attenuation wheel

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