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The spectrum of building block conformers sustains the biophysical properties of clinically-oriented self-assembling protein nanoparticles

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science China materials 2022年第6期65卷 1662-1670页

作者： Eric Voltà-Durán julieta m.sánchez Hèctor-López-Laguna Eloi Parladé Laura sánchez-García Alejandro sánchez-Chardi Ario de marco Ugutz Unzueta Esther Vázquez Antonio Villaverde Institut de Biotecnologia i de Biomedicina Universitat Autònoma de BarcelonaBellaterra 08193BarcelonaSpain Departament de Genètica i de microbiologia Universitat Autònoma de BarcelonaBellaterra 08193BarcelonaSpain CIBER de Bioingeniería Biomateriales y Nanomedicina(CIBER-BBN) Bellaterra 08193BarcelonaSpain Instituto de Investigaciones Biológicas y Tecnológicas(IIBYT)(CONICET-Universidad Nacional de Córdoba) ICTA&Cátedra de Química BiológicaDepartamento de QuímicaFCEFyNUNC.Av.Velez Sarsfield 1611X 5016GCA CórdobaArgentina servei de microscòpia Universitat Autònoma de BarcelonaBellaterra 08193BarcelonaSpain Departament de Biologia Evolutiva Ecologia i Ciències AmbientalsFacultat de BiologiaUniversitat de Barcelona08028 BarcelonaSpain Laboratory for Environmental and Life sciences University of Nova GoricaNova Gorica 5000Slovenia Biomedical Research Institute sant Pau(IIB sant Pau) Sant Antoni Ma Claret 16708025 BarcelonaSpain

Histidine-rich peptides confer self-assembling properties to recombinant proteins through the supramolecular coordination with divalent *** fact allows the cost-effective,large-scale generation of microscopic and macroscopic protein materials with intriguing biomedical *** such materials,resulting from the simple bioproduction of protein building blocks,homomeric nanoparticles are of special value as multivalent interactors and drug ***,we have here identified that the assembly of a given His-tagged protein might render distinguishable categories of self-assembling protein *** fact has been scrutinized through the nanobody-containing fusion proteins Em1-GFP-H6 and A3C8-GFP-H6,whose biosynthesis results in two distinguishable populations of building *** one of them,the assembling and disassembling is controllable by ***,a second population immediately self-assembles upon purification through a non-regulatable pathway,rendering larger nanoparticles with specific biological *** structural analyses of both model proteins and nanoparticles revealed important conformational variability in the building *** fact renders different structural and functional categories of the final soft materials resulting from the participation of energetically unstable intermediates in the oligomerization *** data illustrate the complexity of the Hismediated protein assembling in recombinant proteins but they also offer clues for a better design and refinement of protein-based nanomedicines,which,resulting from biological fabrication,show an architectonic flexibility unusual among biomaterials.

关键词： recombinant proteins self-assembling protein materials nanoparticles biomaterials

Amphibian richness along modified riparian areas of central mexico:conservation priorities

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Ecological Processes 2022年第1期11卷 902-915页

作者： marco Tulio Oropeza‑sánchez Ireri suazo‑Ortuño julieta Benítez‑malvido Erandi monroy‑Hernández Roberto munguía‑steyer Instituto de Investigaciones en Ecosistem.s y sustentabilidad(IIEs) Universidad Nacional Autónoma de MéxicoAntigua Carretera a Pátzcuaro 8701CP 58190 MoreliaMichoacánMexico Instituto de Investigaciones sobre los Recursos Naturales(INIRENA) Universidad Michoacana de San Nicolás de HidalgoAv.San Juanito Itzícuaro S/NCP 58330 MoreliaMichoacánMexico Facultad de Estudios superiores Iztacala(FEs‑Iztacala) Universidad Nacional Autónoma de MéxicoAv.de los Barrios No.1CP 54090 Tlalnepantla de BazEstado de MéxicoMexico.

Background:Land‑use change frequently affects faunistic populations and *** achieve successful conservation strategies,we need suitable information about species distribution and the causes of extinction *** amphibian species depend on riparian vegetation to complete their life *** 41%of amphibian species are globally threatened,and accurate estimations of population size,species richness and the identification of critical habitats are urgently needed *** evaluate the magnitude of changes in species richness and demography,estimations that include detection probability are *** this study,we employed multi‑species occupancy models to estimate detection probability and the effect of land cover type(i.e.,cropland,artificial pasture,secondary and mature forest)in a 500‑m radius on the occupancy probability and richness of diurnal amphibians in 60 riparian zones in the state of michoacán in central ***,we evaluated the potential of the endemic salamander Ambystoma ordinarium as a flagship species for the conservation of other native amphibian ***:We registered a total of 20 amphibian species in the diurnal assemblage,of which 10 species are considered at risk of *** found that cropland was the m.st important land‑use type for explaining amphibian distribu‑tion in riparian zones,with negative effects on m.st amphibian *** found no differences in species richness between zones with and without *** riparian zones occupied by ***,however,we found a higher number of species at risk of ***:Our findings showed negative effects of croplands on the distribution of m.st amphibian *** riparian zones are important for the maintenance of native diurnal amphibian communities and *** can act as a flagship species for the conservation of threatened amphibian species.

关键词： Flagship species Habitat quality Occupancy models salamanders Threatened species

International Collaboration in Brazilian Higher Education

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Frontiers of Education in China 2015年第1期10卷 7-22页

作者： Creso sÁ julieta GRIECO Ontario Institute for studies in Education University of TorontoTorontoOntario M5S 1V6Canada

This paper examines the approach Brazil has taken to promote the internationalization of higher education over the last *** key areas are identified:human resources development,institution building,and international *** analyses of initiatives in these areas demonstrate that Brazil does not follow global trends such as the creation of world-class universities or the pursuit of“excellence initiatives”to help universities reach higher positions in global ***,recent strategies for international collaboration in higher education follow variegated dom.stic goals that encompass the logics of foreign policy and the internal priorities of federal research and education *** nature and limitations of these strategies are discussed.

关键词： Brazilian higher education internationalization higher education policy international collaboration

High-throughput metabarcoding of sAR11 assemblages from the southwest Atlantic shelf and arid Patagonia:richness and associated rank abundance distributions

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Quantitative Biology 2023年第3期11卷 332-342页

作者： Leandro R.Jones julieta m.manrique Laboratorio de Virología y Genética molecular Universidad Nacional dela Patagonia San Juan BoscoTrelew CP 9100Argentina

Background:m.ssively parallel sequencing of environmental DNA allows microbiological studies to be performed in greater detail than was possible with first-generation *** example,it facilitates the use of approaches hitherto largely applied to flora and fauna,such as rank abundance distribution(RAD)***:Here,we set out to advance the knowledge on ***(sAR11)communities from.southern south America using environmental sequences from the open ocean in the Argentine sea,the uncharted Engaño Bay,as well as a river and an oligohaline shallow lake from the Patagonian steppe *** structures of the sAR11 assemblages present in these ecosystems were dissected by direct and rarefaction-based estimates of species richness,and evaluations of the corresponding abundance distributions(ADs),which was addressed by RAD ***:microbial community composition analyses revealed that the studied sAR11 assemblages coexist with 27 bacterial ***11 richness was in general very high,but ADs turned out to be highly *** results were compatible with prior knowledge,and similar to that derived from point estimates of ***,our comprehensive dissection allowed for more detailed quantitative comparisons to be made between the environments surveyed,and revealed differences regarding both richness and the underlying ***:Despite sAR11 assemblages being extremely rich,their ADs are very *** and ADs can vary,not only between fresh and salt water,but also between oceanic and coastal marine *** obtained results provide insights on general topics such as adaptation and the contrast between marine and freshwater radiations.

关键词： sAR11 richness species abundance distribution rank abundance distribution Patagonia Argentina

Role of quorum.sensing in bacterial infections

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World Journal of Clinical Cases 2015年第7期3卷 575-598页

作者： Israel Castillo-Juárez Toshinari maeda Edna Ayerim mandujano-Tinoco maría Tom.s Berenice Pérez-Eretza silvia julieta García-Contreras Thom.s K Wood Rodolfo García-Contreras Colegio de Postgraduados Campus Montecillo Department of Biological Functions and Engineering Kyushu Institute of Technology Epigenetics of Cancer Laboratory Division of Basic ResearchNational Institute of Genomic Medicine Department of microbiology Instituto de Investigación Biomédica de A Coruña(INIBIC)Complexo Hospitalario Universitario de A Coruña(CHUAC)SergasUniversidade da Coruña(UDC)15006 A CoruñaSpain Departamento de microbiología y Parasitología Facultad de MedicinaUniversidad Nacional Autónoma de México Centro médico Coyoacán Department of Chemical Engineering Pennsylvania State UniversityUniversity Park Department of Biochem.stry and molecular Biology Pennsylvania State UniversityUniversity Park

Quorum.sensing(Qs) is cell communication that is widely used by bacterial pathogens to coordinate the expression of several collective traits, including the production of multiple virulence factors, biofilm formation, and swarming motility once a population threshold is reached. several lines of evidence indicate that Qs enhances virulence of bacterial pathogens in animal models as well as in human infections; however, its relative importance for bacterial pathogenesis is still incomplete. In this review, we discuss the present evidence from in vitro and in vivo experiments in animal models, as well as from clinical studies, that link Qs systems with human infections. We focus on two major Qs bacterial models, the opportunistic Gram negative bacteria Pseudomonas aeruginosa and the Gram positive staphylococcus aureus, which are also two of the main agents responsible of nosocomial and wound infections. In addition, Qs communication systems in other bacterial, eukaryotic pathogens, and even immune and cancer cells are also reviewed, and finally, the new approaches proposed to combat bacterial infections by the attenuation of their Qs communication systems and virulence are also discussed.

关键词： Quorum.sensing Virulence Infections Pseudomonas aeruginosa staphylococcus aureus Animal models

Prevalence and intensity of soil-transmitted helminth infections among school-age children in the Cagayan Valley, the Philippines

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Asian Pacific Journal of Tropical medicine 2021年第3期14卷 113-121页

作者： Ryan V.Labana Vimar A.Romero Analette m.Guinto Alvin N.Caril Kimberly D.Untalan Alejandro Jose C.Reboa Khristine L.sandoval Kristel Joy s.Cada Gary Antonio C.Lirio Iris Rowena A.Bernardo Lanieleen Jerah mae Arocha julieta Z.Dungca Department of Biology College of SciencePolytechnic University of the PhilippinesSta.MesaManilathe Philippines Graduate school Centro Escolar UniversityManilathe Philippines Center for Life sciences Research Institute of Science and Technology ResearchPolytechnic University of the PhilippinesSta.MesaManilathe Philippines Department of Nutrition and Dietetics College of SciencePolytechnic University of the PhilippinesSta.MesaManilathe Philippines school of science and Technology Centro Escolar UniversityManilathe Philippines

Objective:To identify the prevalence of soil-transmitted helminthiasis(sTH)among school-age children in the Cagayan Valley,the Philippines,assess their level of awareness on the disease,and determine predisposing factors of the ***:A total of 478 GradesⅢ-Ⅴschool-age children in Pamplona and sanchez-mira school Districts in the Cagayan Valley answered the questionnaire assessing their knowledge,attitude,and practices on sTH,subjected to anthropometric measurements,and provided faecal samples for parasitologic assessment(direct smear,Kato-Katz,and formol-ether concentration techniques).Results:The participants of the study,with 55.86%females,were 8 to 14 years *** nutritional status was assessed‘normal’(84.31%),‘severely wasted’(6.49%),‘wasted’(5.23%),‘overweight’(2.72%),and‘obese’(1.26%).The prevalence of infection with at least 1 sTH species was 25.99%in Pamplona and 19.40%in ***,the prevalence of heavy intensity was 7.11%for Ascaris lumbricoides and 1.67%for Trichuris *** hookworm infections had light *** majority of the school-age children had a low score in the KAP *** knowledge of sTH,‘stunted growth as a symptom of infection’was associated with a lower risk of Ascaris lumbricoides infection(OR 0.448;95%CI 0.212,0.945;P=0.035)while‘playing with soil as a mode of transm.ssion’was associated with an increased risk of Ascaris lumbricoides infection(OR 2.067;95%CI 1.014,4.212;P=0.046).In attitude towards sTH,‘I think I have intestinal worm now’was associated with a higher risk of Ascaris lumbricoides infection(OR 1.681;95%CI 1.061,2.662;P=0.027).Conclusions:The prevalence rate of Ascaris lumbricoides among the school-age children in the Cagayan Valley shows the need to further intensify intervention in the area to meet the threshold set by the World Health *** identified predictors of infection,which concerns the school-age children’s knowledge and attitude toward sTH,can be used in augmenting

关键词： Intestinal worms War on worms Infectious disease Neglected tropical diseases

Pre-treatment prediction of response to peginterferon plus ribavirin in chronic hepatitis C genotype 3

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World Journal of Hepatology 2015年第4期7卷 703-709页

作者： sebastian marciano silvia m Borzi melisa Dirchwolf Ezequiel Ridruejo manuel mendizabal Fernando Bessone maria E sirotinsky Diego H Giunta julieta Trinks Pablo A Olivera Omar A Galdame marcelo O silva Hugo A Fainboim Adrian C Gadano Liver Unit Hospital Italiano de Buenos Aires Hepatology section Hospital R. Rossi La Plata Buenos Aires Argentina Liver Infectious Disease Unit Hospital F.J Muniz Uspallata 2272 Buenos Aires Argentina Hepatology section Department of Medicine Centro de Educacion Medica e Investigaciones Clinicas Norberto Quirno "CEMIC" Avenida Galvan 4102 Buenos Aires Argentina Hepatology and Liver Transplant Unit Hospital Universitario Austral Hepatology Unit Sanatorio del Parque Rosario Argentina HEPATOsUR group Comodoro Rivadavia Chubut Argentina Internal medicine Research Unit Hospital Italiano de Buenos Aires Buenos Aires Argentina Basic science and Experimental medicine Institute Hospital Italiano de Buenos Aires Internal medicine Centro de Educacion Medica e Investigaciones Clinicas Norberto Quirno "CEMIC" Buenos Aires Argentina

AIm: To evaluate pre-treatment factors associated with sustained virological response(sVR) in patients with hepatitis C virus(HCV) genotype 3 treated with peginterferon and ribavirin(RBV). mETHODs: We retrospectively analyzed treatment naive, mono-infected HCV genotype 3 patients treated with peginterferon and RBV. Exclusion criteria included presence of other liver disease, alcohol consumption and African American or Asian ethnicity. The variables collected and compared between patients who achieved an sVR and patients who did not were as follows: gender, age, fibrosis stage, diabetes, body m.ss index,steatosis, INFL3 polymorphism, pre-treatment HCVRNA, type of peginterferon, RBV dose and adherence. REsULTs: A total of 107 patients treated between June, 2004 and march, 2013 were included. mean treatment duration was 25.1(± 1.8) wk. Overall, 58%(62/107) of the patients achieved an sVR and 42%(45/107) did not. In the multivariate logistic regression analysis, pre-treatment HCV-RNA ≥ 600000 UI/m L(OR = 0.375, 95%CI: 0.153-0.919, P = 0.032) and advanced fibrosis(OR = 0.278, 95%CI: 0.113-0.684,P = 0.005) were significantly associated with low sVR rates. In patients with pre-treatment HCV-RNA ≥600000 UI/m L and advanced fibrosis, the probability of achieving an sVR was 29%(95%CI: 13.1-45.2).In patients with pre-treatment HCV-RNA m L and mild to moderate fibrosis, the probability of achieving an sVR was 81%(95%CI: 68.8-93.4).CONCLUsION: In patients with HCV genotype 3infections the presence of advance fibrosis and high pre-treatment viral load might be associated with poor response to peginterferon plus RBV. These patients could benefit the m.st from new direct antiviral agentsbased regim.s.

关键词： sustained virological response Direct antiviral agents sofosbuvir Cirrhosis Viral load

Influence of GsTT1, GsTm1 and GsTP1 Polymorphisms on the Development of Breast Cancer

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Journal of Cancer Therapy 2014年第6期5卷 552-559页

作者： marisol Rodríguez Fernando mejia mariana Lecourtois Victoria Domínguez julieta Castillo Centro de Investigación en Ciencias médicas Universidad Autónoma del Estado de México Toluca de Lerdo México Facultad de Odontología Universidad Autónoma del Estado de México Toluca de Lerdo México Facultad de Química Universidad Autónoma del Estado de México Toluca de Lerdo México

Introduction: Every year, more than 10 million women are diagnosed worldwide with breast cancer. Breast cancer remains the leading cause of death from malignancy in women. In mexico about 12 mexican women die every day from this disease. Glutathione s-transferase (GsT) family consists of a group of isoenzym.s involved in Phase II detoxification of xenobiotics by glutathione conjugation. There have been reports showing GsT polymorphisms as a risk factor for developing cancer. Objective: Determine if the frequencies of polymorphisms of GsTT1, GsTm1 and GsTP1 are associated with breast cancer development. materials and methods: We studied 22 women with breast cancer and a control group of 30 women. DNA was extracted from blood. Polymorphisms of T1 and m1 were identified by multiplex PCR. The identification of GsTP1b (exon 5) and GsTP1c (exon 6), was performed separately by RFLP-PCR, using BsmAI and AciI enzym.s respectively. Results: Frequencies of polymorphisms were as follows: for breast cancer group 33% null GsTT1 vs 40% null in the control, 32% null GsTm1 vs 63% of control;63% heterozygote GsTP1b P1a/P1b and 18% homozygote P1b/P1b, vs 53% heterozygote and 20% homozygote in the control, 41% heterozygote P1a/P1c GsTP1c and 0% homozygote vs 10% heterozygote P1c/P1c and 0% homozygote control. significant differences were found between GsTm1 and GsTP1c polymorphisms with GsTm1 null percentage higher in the control group and a higher frequency of P1c heterozygotes in the cancer group. Conclusion: As this is a pilot study where the results showed no association with breast cancer, further investigation is required on the involvement of GsT genes in neoplasm development.

关键词： Breast Cancer Polymorphisms GsTm1 GsTT1 GsTP1

Biocompatibility and electrochemical evaluation of ZrO_(2) thin films deposited by reactive magnetron sputtering on mgZnCa alloy

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Journal of magnesium and Alloys 2021年第6期9卷 2019-2038页

作者： Benjamín millán-Ram.s Daniela morquecho-marín Phaedra silva-Bermudez David Ramírez-Ortega Osmary Depablos-Rivera julieta García-López mariana Fernández-Lizárraga JoséVictoria-Hernández Dietmar Letzig Argelia Almaguer-Flores sandra E.Rodil Instituto de Investigaciones en materiales Universidad Nacional Autónoma de MéxicoCDMX 04510Mexico Posgrado en Ciencia e Ingeniería de materiales Universidad Nacional Autónoma de MéxicoCDMX 04510Mexico Unidad de Ingeniería de Tejidos Terapia Celular y Medicina RegenerativaInstituto Nacional de Rehabilitación Luis Guillermo IbarraCDMX 14389Mexico Posgrado en Ciencias médicas Odontológicas y de la SaludUniversidad Nacional Autónoma de MéxicoCDMX 04510Mexico Instituto de Ciencias Aplicadas y Tecnologia Universidad Nacional Autónoma de MéxicoCDMX 04510Mexico Posgrado de Doctorado en Ciencias en Biomedicina y Biotecnología molecular Escuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalCDMX 11340Mexico Institute of material and Process Design Helmholtz-Zentrum GeesthachtMax-Planck-Strasse 1Geesthacht 21502Germany magnesium Innovation Centre magIC Helmholtz-Zentrum GeesthachtMax-Planck-Strasse 1Geesthacht 21502Germany Laboratorio de Biointerfases Facultad de OdontologíaDivisión de Estudios de Posgrado e InvestigaciónUniversidad Nacional Autónoma de MéxicoCDMX 04510Mexico

Biodegradable magnesium alloys are prom.sing candidates for temporary fracture fixation devices in orthopedics;nevertheless,its fast degradation rate at the initial stage after implantation remains as one of the main challenges to be ***_(2)-based coatings to reduce the degradation rate of the mg-implants are an attractive solution since they show high biocompatibility and *** this work,the degradation,cytotoxicity,and antibacterial performance of ZrO_(2)thin films deposited by magnetron sputtering on a mg-Zn-Ca alloy was ***-term degradation of ZrO_(2)-coated and uncoated samples was assessed considering electrochemical techniques and H_(2)evolution(gas chromatography).Additionally,long term degradation was assessed by m.ss-loss *** results showed that a 380 nm ZrO_(2)coating reduces the degradation rate and H_(2)evolution of the alloy during the initial 3 days after immersion but allows the degradation of the bare alloy for the *** ZrO_(2)coating does not comprom.se the biocompatibility of the alloy and permits better cell adhesion and proliferation of m.senchymal stem cells directly on its surface,in comparison to the bare ***,the ZrO_(2)coating prevents the adhesion and biofilm formation of ***.

关键词： Zirconium dioxide magnetron sputtering short-term degradation H_(2)evolution