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step-scheme ZnO@Zns hollow microspheres for improved photocatalytic H_(2) production performance

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Chinese Journal of Catalysis 2022年第2期43卷 329-338页

作者： Jie Jiang Guohong Wang Yanchi shao juan Wang shuang Zhou Yaorong su Hubei Key Laboratory of Pollutant Analysis and Reuse Technology College of Chemistry and Chemical EngineeringInstitute for Advanced MaterialsHubei Normal UniversityHuangshi 435002HubeiChina College of New Materials and New Energies Shenzhen Technology UniversityShenzhen 518118GuangdongChina

Constructing a step-scheme heterojunction at the interface between two semiconductors is an efficient way to optimize the redox ability and accelerate the charge carrier separation of a photocatalytic system for achieving high photocatalytic *** this study,we prepared a hierarchical ZnO@Zns step-scheme photocatalyst by incorporating Zns into the outer shell of hollow ZnO microspheres via a simple in situ sulfidation *** ZnO@Zns step-scheme photocatalysts had a large surface area,high light utilization capacity,and superior separation efficiency for photogenerated charge *** addition,the material simulation revealed that the formation of the step-scheme heterojunction between ZnO and Zns was due to the presence of the built-in electric *** study paves the way for design of high-performance photocatalysts for H_(2) production.

关键词： ZnO@Zns Hollow microspheres step-scheme heterojunction Photocatalytic H_(2)production

Heredity and gene mapping of a novel white stripe leaf mutant in wheat

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Journal of Integrative Agriculture 2021年第7期20卷 1743-1752页

作者： LI Hui-juan JIAO Zhi-xin Nl Yong-jing JIANG Yu-mei LI Jun-chang PAN Chao ZHANG Jing sUN Yu-long AN Jun-hang LIU Hong-jie LI Qiao-yun NIU Ji-shan National Centre of Engineering and Technological Research for Wheat/National Key Laboratory of Wheat and Maize Crop science/Henart Agricultural University Zhengzhou 450046P.R.China shangqiu Academy of Agricultural and Forestry sciences Shangqiu 476000P.R.China

spotted leaf(spl)mutant is a type of leaf lesion mimic mutants in *** obtained some lesion mimic mutants from ethyl methane sulfonate(EMs)-mutagenized wheat(Triticum aestivum L.)cultivar Guomai 301(wild type,WT),and one of them was named as white stripe leaf(wsl)mutant because of the white stripes on its *** we report the heredity and gene mapping of this novel wheat mutant *** are many small scattered white stripes on the leaves of wsl throughout its whole growth *** the plants grew,the white stripes became more severe and the necrotic area *** mutant wsl grew only weakly before the jointing stage and gradually recovered after *** length and width of the flag leaf,spike number per plant and thousand-grain weight of wsl were significantly lower than those of the *** analysis indicated that the trait of white stripe leaf was controlled by a recessive gene locus,named as wsl,which was mapped on the short arm of chromosome 6 B by ssR marker *** ssR markers in the F2 population of wsl×Cs were linked to wsl in the order of Xgpw1079–Xwmc104–Xgwm508-wsl–Xgpw7651 at 7.1,5.2,8.7,and 4.4 c M,respectively and three ssR markers in the F2 population of wsl×Jimai 22 were linked to wsl in the order of Xgwm508–Xwmc494–Xgwm518-wsl at 3.5,1.6 and 8.2 c M,*** comparison to the reference genome sequence of Chinese spring(Cs),wsl is located in a 91-Mb region from 88 Mb(Xgwm518)to 179 Mb(Xgpw7651)on chromosome 6 *** wsl is a novel germplasm for studying the molecular mechanism of wheat leaf development.

关键词： wheat(Triticum aestivum L.) mutant white stripe leaf(wsl) heredity gene mapping

Novel Mutations in PsENEN Gene in Two Chinese Acne Inversa Families Manifested as Familial Multiple Comedones and Dowling-Degos Disease

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Chinese Medical Journal 2016年第23期129卷 2834-2839页

作者： Cheng Zhou Guang-Dong Wen Lwin Myint soe Hong-Jun Xu juan Du Jian-Zhong Zhang Department of Dermatology Peking University People's Hospital Beijing 100044 China

Background： Acne inversa （AI）, also called hidradenitis suppurativa, is a chronic, inflammatory, recurrent skin disease of the hair follicle. Familial AI shows autosomal-dominant inheritance caused by mutations in the γ-secretase genes. This study was aimed to identify the specific mutations in the y-secretase genes in two Chinese families with AI. Methods： In this study, two Chinese families with AI were investigated. All the affected individuals in the two families mainly manifested with inultiple comedones, pitted scars, and a few inflammatory nodules on their face, neck, trunk, axilla, buttocks, upper arms, and thighs. Reticulate pigmentation in the flexures areas resembled Dowling-Degos disease clinically and pathologically. In addition, one of the affected individuals developed anal canal squamous cell carcinoma. Molecular mutation analysis of γ-secretase genes including PsENEN, PsENI, and NCsTN was performed by polymerase chain reaction and direct DNA sequencing. Results： Two novel mutations of PsENEN gene were identified, including a heterozygous missense mutation c. 194T〉G （p.L65R） and a splice site mutation c. 167-2A〉G. Conclusions： The identification of the two mutations could expand the spectrum of mutations in the γ-secretase genes underlying AI and provide valuable information for further study of genotype-phenotype correlations.

关键词： Acne lnversa Coinedones Dowling-Degos Disease Hidradenitis suppurativa PsENEN squamous Cell Carcinoma

P19 of Tomato Bushy stunt Virus suppresses RNA silencing Induced by short Hairpin RNA in Mammal Cells

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中国病毒学 2007年第3期22卷 199-206页

作者： Wei-xian CHEN　 juan CHEN　 Zhen-zhen ZHANG　 Ai-long HUANG　 Institute for Viral Hepatitis Key Laboratory on Infectious Disease of Ministry of Education Chongqing University of Medical Science Chongqing 400016 China

To counteract the immune system in parasitic hosts,some viruses encode proteins to suppress the RNA interference(RNAi)*** this report,we established two RNAi systems to be easily observed with strong and obvious *** function of the P19 of tomato bushy stunt virus,which suppresses RNAi in mammal cells,was then studied using these two *** hairpin RNAs targeting green fluorescence protein(pshRNA-GFP)and firefly luciferase(pshRNA-luc)were designed and inserted into a eukaryotic transcriptional vector pTZU6+1,*** shRNA expressing vectors were co-transfected with plasmids containing the target gene with or without *** GFP expression level was assayed by fluorescence microscopy,Western blotting and *** luciferase expression level was analyzed by the dual-luciferase assay *** designed in this study down-regulated the target gene specifically and efficiently,with a decrease of expression of both genes of about 70%,*** P19 was introduced into the RNAi systems,the expression of both GFP and the luciferase were mostly recovered compared with the control *** RNAi systems of GFP and luciferase were constructed successfully,demonstrating that P19 of tomato bushy stunt virus has the ability to counteract the RNAi effect induced by shRNA in mammal cells.

关键词：哺乳动物 RNA干涉番茄丛矮病毒 P19 RNA抑制

VEGFR2 inhibition hampers breast cancer cell proliferation via enhanced mitochondrial biogenesis

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Cancer Biology & Medicine 2021年第1期18卷 139-154页

作者： Hao Ni Min Guo Xuepei Zhang Lei Jiang shuai Tan juan Yuan Huanhuan L Cui Yanan Min Junhao Zhang susanne schlisio Chunhong Ma Wangjun Liao Monica Nister Chunlin Chen shuijie Li Nailin Li Department of Gynaecology and Obstetrics Nanfang HospitalSouthern Medical UniversityGuangzhou 510515China Karolinska Institutet Department of Medicine-SolnaClinical Pharmacology GroupKarolinska University Hospital-SolnaStockholm 17176Sweden Karolinska Institutet Department of Oncology-PathologyBio ClinicumSolna 17164Sweden state Key Laboratory of Bioorganic and Natural Products Chemistry Center for Excellence in Molecular SynthesisShanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 200032China Department of Pathology Zhejiang Provincial Key Laboratory of PathophysiologyNingbo University School of MedicineNingbo 315211China Department of Hematology Affiliated Hospital of Jining Medical UniversityJining 272000China Department of Cell and Molecular Biology Stockholm 17177Sweden Karolinska Institutet Department of MicrobiologyTumor and Cell BiologyStockholm 17177Sweden shandong University Cheeloo Medical College School of Basic MedicineDepartment of ImmunologyJinan 250000China Department of Oncology Nanfang HospitalSouthern Medical UniversityGuangzhou 510515China

Objective:Vascular endothelial growth factor(VEGF),apart from its predominant roles in angiogenesis,can enhance cancer cell proliferation,but its mechanisms remain *** purpose of the present study was therefore to identify how VEGF regulates cancer cell ***:VEGF effects on cancer cell proliferation were investigated with the VEGF receptor 2 inhibitor,Ki8751,and the breast cancer cell lines,MCF-7 and MDA-MB-231,using flow cytometry,mass spectrometry,immunoblotting,and confocal *** were analyzed using one-way analysis of variance followed by Tukey’s multiple comparison ***:VEGF blockade by Ki8751 significantly reduced cancer cell proliferation,and enhanced breast cancer cell *** spectrometric analyses revealed that Ki8751 treatment significantly upregulated the expression of mitochondrial proteins,suggesting the involvement of mitochondrial *** microscopy and flow cytometric analyses showed that Ki8751 treatment robustly increased the mitochondrial masses of both cancer cells,induced endomitosis,and arrested cancer cells in the high aneuploid ***2 knockdown by sh RNAs showed similar effects to those of Ki8751,confirming the specificity of Ki8751 *** mitochondrial biogenesis increased mitochondrial oxidative phosphorylation and stimulated reactive oxygen species(ROs)production,which induced cancer cell ***,Ki8751 treatment downregulated the phosphorylation of Akt and PGC1α,and translocated PGC1αinto the *** PGC1αalterations increased mitochondrial transcription factor A(TFAM)expression and subsequently increased mitochondrial ***:VEGF enhances cancer cell proliferation by decreasing Akt-PGC1α-TFAM signaling-mediated mitochondrial biogenesis,ROs production,and cell *** findings suggested the anticancer potential of Ki8751 via increased mitochondrial biogenesis and ROs production.

关键词： Breast cancer VEGF VEGFR2 mitochondria TFAM ROs apoptosis

Effect of Lactobacillus rhamnosus GG supernatant on serotonin transporter expression in rats with post-infectious irritable bowel syndrome

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World Journal of Gastroenterology 2018年第3期24卷 338-350页

作者： Ya-nan Cao Li-juan Feng Yuan-Yuan Liu Kui jiang Mao-jun Zhang Yi-xin Gu Bang-Mao Wang jia Gao Ze-Lan Wang Yu-Ming Wang Department of Gastroenterology and Hepatology Tianjin Medical University General Hospital Department of Functional Division Xingtai People’s Hospital National Institute for Commu-nicable Disease Control and Prevention Chinese Center for Disease Control and Prevention

AIM To evaluate the effect of Lactobacillus rhamnosus GG supernatant(LGG-s) on the expression of serotonin transporter(sERT) in rats with post-infectious irritable bowel syndrome(PI-IBs).METHODs Campylobacter jejuni 81-176(1010 CFU/m L) was used to induce intestinal infection to develop a PI-IBs model. After evaluation of the post-infectious phase by biochemical tests, Dn A agarose gel electrophoresis, abdominal withdrawal reflex(AWR) test, and the intestinal motility test, four PI-IBs groups received different concentrations of LGG-s for 4 wk. The treatments were maintained for 1.0, 2.0, 3.0 or 4.0 wk during the experiment, and the colons and brains were removed for later use each week. sERT m Rn A and protein levels were detected by real-time PCR and Western blot, *** The levels of sERT m Rn A and protein in intestinal tissue were higher in rats treated with LGG-s than in control rats and PI-IBs rats gavaged with PBs during the whole study. Undiluted LGG-s up-regulated sERT m Rn A level by 2.67 times compared with the control group by week 2, and sERT m Rn A expression kept increasing later. Double-diluted LGG-s was similar to undiluted-LGG-s, resulting in high levels of sERT m Rn A. Triple-diluted LGG-s up-regulated sERT m Rn A expression level by 6.9-times compared with the control group, but sERT m Rn A expression decreased rapidly at the end of the second week. At the first week, sERT protein levels were basically comparable in rats treated with undiluted LGG-s, double-diluted LGG-s, and triplediluted LGG-s, which were higher than those in the control group and PBs-treated PI-IBs group. sERT protein levels in the intestine were also comparable in rats treated with undiluted LGG-s, double-diluted LGG-s, and triple-diluted LGG-s by the second and third weeks. sERT m Rn A and protein levels in the brain had no statistical difference in the groups during the *** LGG-s can up-regulate sERT m Rn A and protein levels in intestinal t

关键词： serotonin transporter intestinal infection Lactobacillus rhamnosus supernatant Irritable bowel syndrome

重复荷载下沥青混合料变形的粘弹性有限元分析

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华南理工大学学报（自然科学版） 2009年第11期37卷 12-16页

作者：张丽娟陈页开华南理工大学土木与交通学院广东广州510640

沥青混合料是典型的粘弹性材料,广义Maxwell粘弹性模型能很好地描述沥青混合料的变形规律.文中通过拟合AC-13C基质、改性沥青混合料的静载蠕变试验数据获得广义Maxwell模型的Prony系列系数,采用加载0.1 s、卸载0.9 s的半正弦波间歇荷载... 详细信息

沥青混合料是典型的粘弹性材料,广义Maxwell粘弹性模型能很好地描述沥青混合料的变形规律.文中通过拟合AC-13C基质、改性沥青混合料的静载蠕变试验数据获得广义Maxwell模型的Prony系列系数,采用加载0.1 s、卸载0.9 s的半正弦波间歇荷载模拟路面实际的车辆荷载,对重复荷载作用下的沥青混合料进行粘弹性有限元分析,预测出重复荷载作用下AC-13C基质、改性沥青混合料的变形,与实测变形相比具有很好的一致性.粘弹性有限元方法预测出的应变包括弹性应变和蠕变应变,而蠕变应变直接导致永久变形的产生,通过分析蠕变应变可以判别沥青混合料的弹性恢复能力.

关键词：沥青混合料粘弹性广义Maxwell模型有限元分析重复荷载变形

权力与公平:社会网络嵌入对农资零售商知识转移影响机理的实证研究——基于sEM的传统渠道和电商渠道情境的差异分析

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管理工程学报 2019年第4期33卷 10-18页

作者：孙娟李艳军南昌大学经济管理学院江西南昌330031 华中农业大学经济管理学院湖北武汉430070 湖北农村发展研究中心湖北武汉430070

以农资传统渠道和电商渠道交织渗透为研究背景,本文从渠道关系视角深入探究了农资零售商社会网络嵌入对知识转移行为的影响机理,并实证比较了权力和公平两种不同渠道关系在知识转移行为形成中的作用,同时对比分析了传统和电商两种渠道... 详细信息

以农资传统渠道和电商渠道交织渗透为研究背景,本文从渠道关系视角深入探究了农资零售商社会网络嵌入对知识转移行为的影响机理,并实证比较了权力和公平两种不同渠道关系在知识转移行为形成中的作用,同时对比分析了传统和电商两种渠道情境下社会网络嵌入对知识转移行为作用机理之间的差异性。研究表明:(1)社会网络嵌入通过渠道关系的中介作用而对知识转移行为产生显著影响;(2)结构嵌入对渠道权力关系的影响效应最大、关系嵌入对渠道权力关系的影响次之、认知嵌入则对渠道权力关系无显著影响,而关系嵌入却对渠道公平关系的影响效应最大、认知嵌入对渠道公平关系的影响次之、结构嵌入则对渠道公平关系的影响最低;(3)渠道权力关系对显性知识转移的影响略大,而渠道公平关系对隐性知识转移的影响较大;(4)传统情境下由结构嵌入所引发的渠道权力关系使得农资零售商更多地表现为显性知识转移行为,电商情境下由关系嵌入所引发的渠道公平关系使得农资零售商更多地表现为隐性知识转移行为。本研究对探析现有渠道关系与知识转移之间联系的研究分歧所产生的内在原因具有重要的理论意义,对农资企业和政府机构有效提高知识转移绩效亦具有一定的参考价值。

关键词：知识转移社会网络渠道关系农资零售商多群组结构方程模型

The Long-Lasting Effect of Neonatal Repeated sevof lurane Anesthesia on the synaptic Plasticity in the Rat Hippocampal CA1 Field

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神经药理学报 2017年第3期7卷 59-60页

作者： LIANG Xiao-li ZHANG Yi ZHANG Chao TANG Chun-chun WANG Yi REN juan-juan CHEN Xi ZHANG Yu ZHU Zhao-qiong Guizhou Key Laboratory of Anesthesia and Organ Protection Department of Anesthesiology the Affiliated Hospital of Zunyi Medical University

Objective:In the present study,from the functional perspective,the effect of neonatal repeated sevoflurane anesthesia on the synaptic plasticity at juvenile and adult age was observed in the sD rat model. Also,the related mechanism was probed in depth. Methods:*** and establishing models for repeated inhaled anesthesia:32 healthy P7 sprague-Dawley（sD） rats,weight 15;9 g,female and male either,were assigned into experimental group（group sev） and control group（group C）(n=16). Each group was divided into 2 subgroups:juvenile group（P37） and adult group（P97）. During juvenile stage,group sev inhaled 2.6% sevoflurane and carrier gas（1 L·min-1 Air+1 L·min-1 O2） for 2 h at day 7,14 and 21 repeatedly,while group C inhaled carrier gas merely in the same time points. 2. Electrophysiological experiment:On postnatal day 37（P37） and P97,8 rats were extracted from each groups respectively for electrophysiological recording in vivo.（1）The tip of recording electrode located in the pyramidal cell body layer of CA1,the bipolar stimulating electrode was inserted into the ipsilateral schaffer collaterals.（2）A test stimulation with duration of 0.2 ms and intensity of 3;m A was given every 10 s to elicit the adequate wave shape. 50% of intensity to elicit maximum amplitude of population spike was regarded as intensity of basic stimulation. The response to basic stimulation was recorded for 30 min stably.（3） 5 min power spectrum analysis was implemented before basal stimulation,before HFs and after the whole recording to maintain the similar anesthetic level of each sample in the experiment.（4）After 30 min steady basal stimulus,paired stimulation with different interstimulus interval of 25,50,75,100,150,200 and 250 ms were implemented. High frequency stimulation（HFs） was performed and thereafter shifted into basal stimulation for subsequent 90 min recording.（5）The population spike amplitude（PsA）,slope of field excitatory postsynaptic potential（f EPsP） and the response to paired stimu

关键词： synaptic plasticity neonatal period sevoflurane hippocampal neuron learning and memory