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云南省弥勒县2009年蚊媒病毒分离和鉴定

中国媒介生物学及控制杂志 2012年第5期23卷 402-405页

作者：冯云陈卫武杨卫红章域震杨杜鹃刘芬张娟王丕玉白鹏飞窦友剑李继辉张海林云南省地方病防治所/云南省病毒立克次体研究中心云南大理671000 弥勒县疾病预防控制中心云南弥勒652300 云南省寄生虫病防治所

目的了解云南省红河州弥勒县蚊虫中虫媒病毒的分布特点,为虫媒病毒病防治提供科学依据。方法 2009年7月在弥勒县采集蚊虫标本,用细胞培养法分离病毒,对病毒分离物进行分子生物学鉴定。结果从采集到的三带喙库蚊4400只和中华按蚊2200只... 详细信息

目的了解云南省红河州弥勒县蚊虫中虫媒病毒的分布特点,为虫媒病毒病防治提供科学依据。方法 2009年7月在弥勒县采集蚊虫标本,用细胞培养法分离病毒,对病毒分离物进行分子生物学鉴定。结果从采集到的三带喙库蚊4400只和中华按蚊2200只中分离到9株阳性分离物,经分子生物学鉴定,7株分离物为流行性乙型脑炎(乙脑)病毒(JEV),均分离自三带喙库蚊;2株分离物为淡色库蚊浓核病毒(CppDNV),分离自三带喙库蚊和中华按蚊。结论首次在红河州弥勒县分离到乙脑病毒,从病原学证实弥勒县是乙脑流行区。流行株同源性和分子遗传进化分析证实为基因Ⅰ型乙脑病毒,为滇东南地区首次分离。

关键词：蚊虫虫媒病毒流行性乙型脑炎病毒淡色库蚊浓核病毒

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南方水稻黑条矮缩病综合防治技术研究

西南农业学报 2017年第12期30卷 2705-2710页

作者：于文娟王建钟雪莲李红松姬红丽周雪平彭云良农业部西南作物有害生物综合治理重点实验室/四川省农业科学院植物保护研究所四川成都610066 中国农业科学院植物保护研究所北京100193 广西壮族自治区桂林市兴安县农业局广西兴安541300 中国水稻研究所/水稻生物学国家重点实验室浙江杭州311401

【目的】本文研究了杂交稻南方水稻黑条矮缩病综合防治技术。【方法】在广西兴安县病圃,调查了25%吡虫啉拌种、播种后40目防虫网覆盖、出苗后15 d 25%噻嗪酮喷雾以及移栽前2 d 25%吡蚜酮喷雾带药移栽等单项措施及其各措施不同组合,对中... 详细信息

【目的】本文研究了杂交稻南方水稻黑条矮缩病综合防治技术。【方法】在广西兴安县病圃,调查了25%吡虫啉拌种、播种后40目防虫网覆盖、出苗后15 d 25%噻嗪酮喷雾以及移栽前2 d 25%吡蚜酮喷雾带药移栽等单项措施及其各措施不同组合,对中浙优8号、H两优991和Taichung Native(TN1)等3个水稻品种乳熟后期南方水稻黑条矮缩病发病株率的影响,以出现高位不育分蘖及明显矮化症状为病株标准。【结果】不同品种各处理中发病株率数据表明,中浙优8号对南方水稻黑条矮缩病具有抗性,而H两优991和TN1均为感病品种。在不同防治措施组合中,中浙优8号的平均发病株率为6.97%,各防治措施及其组合的相对防效在26.08%~75.38%,其中以防虫网覆盖+25%吡蚜酮带药移栽的防治效果最高;不同处理间H两优991的发病株率在9.63%~22.05%,在不同防治措施间无显著差异(P>0.05)但均显著低于本品种的空白对照,其中7个防治处理的平均病株率与中浙优8号空白对照相当(P>0.05);不同处理下的TN1发病株率均为100%。【结论】药剂拌种、防虫网覆盖结合带药移栽可以保护抗、感品种免受南方水稻黑条矮缩病侵袭;抗病品种与苗期全程保护技术相结合,可以更有效地控制病害发生。

关键词：水稻南方水稻黑条矮缩病抗性杀虫剂防虫网覆盖带药移栽

两种非贵金属烤瓷冠对基牙龈沟液量及其成分的影响研究

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临床口腔医学杂志 2013年第1期29卷 25-28页

作者：季娟娟杨杨黄大海王祥芸彭蓓温红萍张晓渝云南省第二人民医院口腔科云南昆明650021

目的:探讨钛合金和钴铬合金烤瓷冠对基牙牙周组织的影响。方法:选择需要制作钛合金和钴铬合金烤瓷冠的临床病例各50例,于修复前,戴临时冠1周,戴牙1个月、3个月、6个月提取龈沟液,检测其质量、内毒素、碱性磷酸酶(ALP)及天门冬氨酸基转移... 详细信息

目的:探讨钛合金和钴铬合金烤瓷冠对基牙牙周组织的影响。方法:选择需要制作钛合金和钴铬合金烤瓷冠的临床病例各50例,于修复前,戴临时冠1周,戴牙1个月、3个月、6个月提取龈沟液,检测其质量、内毒素、碱性磷酸酶(ALP)及天门冬氨酸基转移酶(AST)活性含量。结果:钛合金和钴铬合金烤瓷组戴临时冠1周时龈沟液量、内毒素、ALP及AST量均有所增高(P0.05)。结论:两种非贵金属烤瓷冠对基牙牙周组织无不良影响。

关键词：烤瓷冠龈沟液内毒素 ALP AST

Expert Consensus on Pathological Diagnosis of Intrahepatic Cholangiocarcinoma(2022 version)

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Journal of Clinical and Translational Hepatology 2023年第7期11卷 1553-1564页

作者： Han Wang Jun Chen Xin Zhang Xia sheng Xiao-Yan Chang jie Chen Min-Shan Chen Hui Dong Guang-jie Duan He-Ping Hu Zhi-Yong Huang Wei-Dong jia Xiao-Qing jiang Dong Kuang Shan-Shan Li Zeng-Shan Li Chang-Li Lu Shu-Kui Qin Xue-Shan Qiu Li-juan Qu Chun-Kui Shao Feng shen Guo-Ming Shi Su-sheng Shi yu-Jun Shi Hui-Chuan Sun Xiao-Dong Teng Bin Wang Zhan-Bo Wang Tian-Fu Wen jia-Mei yang Qiao-Qiao yang sheng-Long Ye hong-Fang Yin Zhen-Gang yuan jing-Ping yun Feng-Lin Zang hong-Qi Zhang Li-hong Zhang jing-Min Zhao jian Zhou Wei-Xun Zhou jia Fan Xiao-Ping Chen Wan Yee Lau yuan ji Wen-Ming Cong Chinese Society of Liver Cancer of Chinese Anti-Cancer Association Digestive Disease Group of Chinese Society of Pathology,Chinese Medical Association Chinese Society of Pathology of Chinese Anti-Cancer Association Hepatic Surgery Group of Chinese Society of Surgery,Chinese Medical Association Biliary Tract Tumor Committee of China Anti-Cancer Association Chinese Society of Clinical Oncology IDepartment of Pathology Eastern Hepatobilary Surgery HospitalNaval Medical UniversityShanghaiChina Department of Pathology the Affiliated Drum Tower HospitalNanjing University Medical SchoolNanjingJiangsuChina Department of Pathology Zhongshan HospitalFudan UniversityShanghaiChina Department of Pathology Minhang HospitalFudan Uni-versityShanghaiChina Department of Pathology Peking Union Medical College HospitalChinese Academy of Medical Sci-ences and Peking Union Medical CollegeBeijingChina Department of Liver Surgery Sun Yat-Sen University Cancer CenterGuangzhouGuangdongChina Department of Pathology The First Affiliated HospitalArmy Medical UniversityChongqingChina Department of Hepatobiliary Medicine Eastern Hepatobiliary Surgery HospitalNaval Medical UniversityShanghaiChina Hepatic Surgery Center Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWu-hanHubeiChina Department of General Surgery The First Affliated Hospital of USTCDivision of Life Science and Medi-cineUniversity of Science and Technology of ChinaHefeiAnhuiChina Department of Biliary Surgery I Eastern Hepatobil-iary Surgery HospitalNaval Medical UniversityShanghaiChina Department of Pathology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiChina Department of Pathology The First Afiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenanChina Department of Pathology Xijing Hospi-talAir Force Medical UniversityXi'anShaanxiChina Department of Pathology West China HospitalSichuan UniversityChengduSichuanChina Cancer Center of jinling Hospital Nanjing University of Chinese MedicineNanjingJiangsuChina Department of Pathology The First Affiliated HospitalChina Medical UniversityShenyangLiaoningChina Department of Pathology The 900 Hospital of the Chinese People's Liberation Army Joint Logistics TeamFuzhouFujianChina Department of Pathology The Third Affiliated HospitalSun Yat-Sen UniversityGuangz

Intrahepatic cholangiocarcinoma(iCCA)can originate from the large bile duct group(segment bile ducts and area bile ducts),small bile duct group(septal bile ducts and interlobular bile ducts),and terminal bile duct group(bile ductules and canals of Hering)of the intrahepatic biliary tree,which can be histopathological corresponding to large duct type iCCA,small duct type iCCA and iCCA with ductal plate malformation pattern,and cholangiolocarcinoma,*** challenge in pathological diagnosis of above subtypes of iCCA falls in the distinction of cellular morphologies,tissue structures,growth patterns,invasive behaviors,immunophenotypes,molecular mutations,and surgical *** these reasons,this expert consensus provides nine recommendations as a reference for standardizing and refining the diagnosis of pathological subtypes of iCCA,mainly based on the 5th edition of the World Health Organization Classification of Tumours of the Digestive System.

关键词： Intrahepatic cholangiocarcinoma Pathology Histological type Immunohistochemistry Molecular variation

两种普通烤瓷冠对龈沟液内酶活性及内毒素含量的影响

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临床口腔医学杂志 2014年第1期30卷 22-24页

作者：黄大海杨杨季娟娟王祥芸彭蓓张晓渝温红平云南省第二人民医院云南昆明650021

目的:检测钛合金与镍铬合金烤瓷冠龈沟液内酶活性及内毒素含量,了解其对龈沟液内酶活性及内毒素含量的影响。方法:选择需要制作镍铬合金和钛合金烤瓷冠的患者各30例,分别在修复前、戴临时冠1周、戴烤瓷冠1月、3月、6月提取烤瓷牙龈沟液... 详细信息

目的:检测钛合金与镍铬合金烤瓷冠龈沟液内酶活性及内毒素含量,了解其对龈沟液内酶活性及内毒素含量的影响。方法:选择需要制作镍铬合金和钛合金烤瓷冠的患者各30例,分别在修复前、戴临时冠1周、戴烤瓷冠1月、3月、6月提取烤瓷牙龈沟液,称其质量,检测龈沟液内AST、ALP活性及内毒素含量。结果:两种烤瓷冠龈沟液内ALP、AST活性值及内毒素值戴临时冠1周后上升,与牙体预备前比较,差异有统计学意义(P0.05);两组烤瓷冠龈沟液内ALP、AST活性值及内毒素值在不同时间段进一步两两比较(q检验),镍铬组ALP、AST活性值及内毒素值高于钛合金组,差异有统计学意义(P<0.05)。两种烤瓷冠龈沟液值比较,临时戴牙一周后突然上升,一个月之后下降并趋于平缓,与修复前无显著性差异。结论:两种普通金属烤瓷冠对龈沟内的ALP、AST活性及内毒素的量有一定的影响,但钛合金烤瓷冠较镍铬烤瓷冠影响小,两种烤瓷冠修复6月后龈沟液的量与修复前无显著性差异。

关键词：龈沟液 ALP AST 内毒素

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人-兔异种核移植构建克隆胚的实验研究

Zoological Research 2005年第4期26卷 416-421页

作者：季静娟郭通航童先宏骆丽华周桂香傅应云刘雨生安徽医科大学附属省立医院生殖中心安徽合肥230001

“治疗性克隆”是人类最关注的课题之一,而人体细胞核移植是治疗性克隆的基础和前提。异种核移植的方法虽已被引入人体细胞克隆胚的构建,但供体细胞的类型、培养代数及准备方法与其效率之间的关系尚有待探讨。本实验以不同培养代数和不... 详细信息

“治疗性克隆”是人类最关注的课题之一,而人体细胞核移植是治疗性克隆的基础和前提。异种核移植的方法虽已被引入人体细胞克隆胚的构建,但供体细胞的类型、培养代数及准备方法与其效率之间的关系尚有待探讨。本实验以不同培养代数和不同准备方法的人卵丘细胞、皮肤成纤维细胞和软骨细胞为供体构建了克隆胚,对其发育情况的比较表明,以卵丘细胞为供体时重构胚的体外发育率高于其余二者,差异显著(P<0.05);不同培养代数的成纤维细胞克隆胚和不同冷藏天数供体细胞克隆胚体外发育率无明显差异。此外,本实验还尝试用荧光原位杂交法检测所构建的异种克隆胚核遗传物质的来源,结果显示来自人体细胞。本研究表明,人-兔异种核移植构建克隆胚切实可行;体细胞的类型与核移植效率相关;供体细胞的体外培养传代对克隆胚的发育并无影响;而冷藏是一种简便有效的供体细胞准备方法;此外,用FISH方法对重构胚进行核遗传物质的鉴定切实可行。

关键词：治疗性克隆核移植体细胞核移植异种克隆胚胎

The first data release(DR1) of the LAMOST regular survey

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Research in Astronomy and Astrophysics 2015年第8期15卷 1095-1124页

作者： A-Li Luo Yong-Heng Zhao Gang Zhao Li-Cai Deng Xiao-Wei Liu Yi-Peng jing Gang Wang Hao-Tong Zhang jian-Rong Shi Xiang-Qun Cui Yao-Quan Chu Guo-Ping Li Zhong-rui Bai yue Wu Yan Cai Shu-yun Cao Zi-Huang Cao Jeffrey L.Carlin Hai-yuan Chen jian-Jun Chen Kun-Xin Chen Li Chen Xue-Lei Chen Xiao-Yan Chen Ying Chen Norbert Christlieb jia-ru Chu Chen-Zhou Cui Yi-Qiao Dong Bing Du Dong-Wei Fan Lei Feng jian-Ning Fu Peng Gao Xue-Fei Gong Bo-Zhong Gu Yan-Xin Guo Zhan-Wen Han Bo-Liang He jin-Liang Hou Yong-Hui Hou Wen Hou hong-Zhuan Hu Ning-sheng Hu Zhong-Wen Hu Zhi-Ying Huo Lei jia Fang-Hua jiang Xiang jiang Zhi-Bo jiang Ge jin Xiao Kong Xu Kong Ya-juan Lei Ai-Hua Li Chang-Hua Li Guang-Wei Li Hai-Ning Li jian Li Qi Li Shuang Li Sha-Sha Li Xin-Nan Li Yan Li Yin-Bi Li Ye-Ping Li yuan Liang Chien-Cheng Lin Chao Liu Gen-Rong Liu Guan-Qun Liu Zhi-Gang Liu Wen-Zhi Lu yu Luo Yin-Dun Mao Heidi Newberg ji-Jun Ni Zhao-Xiang Qi Yong-Jun Qi Shi-Yin shen Huo-Ming Shi jing Song Yi-Han Song Ding-Qiang Su hong-Jun Su Zheng-hong Tang Qing-sheng Tao yuan Tian Dan Wang Da-Qi Wang Feng-Fei Wang Guo-Min Wang Hai Wang hong-Chi Wang jian Wang jia-Ning Wang jian-Ling Wang jian-Ping Wang Jun-Xian Wang Lei Wang Meng-Xin Wang Shou-Guan Wang Shu-Qing Wang Xia Wang Ya-Nan Wang You Wang yue-Fei Wang You-Fen Wang Peng Wei Ming-Zhi Wei hong Wu Ke-Fei Wu Xue-Bing Wu yu-Zhong Wu Xiao-Zheng Xing Ling-Zhe Xu Xin-Qi Xu Yan Xu Tai-sheng Yan De-Hua yang Hai-Feng yang Hui-Qin yang Ming yang Zheng-Qiu Yao Yong yu Hui yuan Hai-Bo yuan Hai-Long yuan Wei-Min yuan Chao Zhai En-Peng Zhang Hua-Wei Zhang jian-Nan Zhang Li-Pin Zhang Wei Zhang Yong Zhang Yan-Xia Zhang Zheng-Chao Zhang Ming Zhao Fang Zhou Xu Zhou jie Zhu Yong-Tian Zhu Si-Cheng Zou Fang Zuo National Astronomical Observatories Chinese Academy of Sciences Department of Astronomy Peking University Shanghai Astronomical Observatory Chinese Academy of Sciences Nanjing Institute of Astronomical Optics & Technology National Astronomical ObservatoriesChinese Academy of Sciences University of Science and Technology of China Department of Physics Applied Physics and AstronomyRensselaer Polytechnic InstituteTroyNY 12180USA Department of Physics and Astronomy Earlham CollegeRichmondIN 47374USA Universitt Heidelberg Zentrum für AstronomieLandessternwarteKnigstuhl 1269117 HeidelbergGermany Department of Astronomy Beijing Normal University yunnan Observatories Chinese Academy of Sciences Purple Mountain Observatory Chinese Academy of Sciences

The Large sky Area Multi-Object Fiber Spectroscopic Telescope（LAMOST） general survey is a spectroscopic survey that will eventually cover approximately half of the celestial sphere and collect 10 million spectra of stars, galaxies and QSOs. Objects in both the pilot survey and the first year regular survey are included in the LAMOST DR1. The pilot survey started in October 2011 and ended in June 2012, and the data have been released to the public as the LAMOST Pilot Data Release in August 2012. The regular survey started in September 2012, and completed its first year of operation in June 2013. The LAMOST DR1 includes a total of 1202 plates containing 2 955 336 spectra, of which 1 790 879 spectra have observed signalto-noise ratio（SNR） ≥ 10. All data with SNR ≥ 2 are formally released as LAMOST DR1 under the LAMOST data policy. This data release contains a total of 2 204 696 spectra, of which 1 944 329 are stellar spectra, 12 082 are galaxy spectra and 5017 are quasars. The DR1 not only includes spectra, but also three stellar catalogs with measured parameters： late A,FGK-type stars with high quality spectra（1 061 918 entries）, A-type stars（100 073 entries）, and M-type stars（121 522 entries）. This paper introduces the survey design, the observational and instrumental limitations, data reduction and analysis, and some caveats. A description of the FITS structure of spectral files and parameter catalogs is also provided.

关键词： techniques: spectroscopic survey—data release—catalog

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临时冠对龈沟液量及其成分的影响

临床口腔医学杂志 2013年第4期29卷 199-201页

作者：杨杨季娟娟黄大海王祥芸彭培张晓渝温红萍昆明医科大学第四附属医院口腔修复科云南昆明650021

目的:检测烤瓷冠修复前、戴临时冠后1周龈沟液量及AST、ALP活性、内毒素含量,评价临时冠对牙周组织的影响。方法:选择需要制作烤瓷冠的患者30例,分别于修复前、戴临时冠1周、提取其龈沟液检测质量及AST、ALP活性及内毒素含量,分析龈沟... 详细信息

目的:检测烤瓷冠修复前、戴临时冠后1周龈沟液量及AST、ALP活性、内毒素含量,评价临时冠对牙周组织的影响。方法:选择需要制作烤瓷冠的患者30例,分别于修复前、戴临时冠1周、提取其龈沟液检测质量及AST、ALP活性及内毒素含量,分析龈沟液的质量及AST、ALP活性及内毒素含量变化,评估临时冠对牙周组织的影响。结果:戴临时冠1周时龈沟液量、AST、ALP及内毒素量均高于牙体预备前。结论:戴临时冠1周时龈沟液量、AST、ALP及内毒素量均增高;提示临时冠制作材料和方法均需要进一步探讨。

关键词：临时冠 AST ALP 内毒素龈沟液

Rab7真核表达载体的构建及其在小鼠巨噬细胞RAW264.7细胞中的表达

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哈尔滨医科大学学报 2012年第5期46卷 436-440页

作者：蔡富娟谢基明康鸿斌孙晓琳王娜李云旭王玉珍内蒙古农业大学生命科学学院内蒙古呼和浩特010018 内蒙古自治区医院检验科内蒙古呼和浩特010017 内蒙古医学院第一附属医院普外科内蒙古呼和浩特010010

目的构建Rab7的真核表达载体,研究其在小鼠巨噬细胞RAW264.7中的表达。方法 RT-PCR方法分析Rab7在组织和细胞中的表达。以RAW264.7细胞的cDNA为模板,根据GenBank公布的小鼠Rab7全长序列设计引物,扩增Rab7基因的开放阅读框。将其与真核... 详细信息

目的构建Rab7的真核表达载体,研究其在小鼠巨噬细胞RAW264.7中的表达。方法 RT-PCR方法分析Rab7在组织和细胞中的表达。以RAW264.7细胞的cDNA为模板,根据GenBank公布的小鼠Rab7全长序列设计引物,扩增Rab7基因的开放阅读框。将其与真核表达载体pcDNA3.1(-)连接,构建pcDNA-Rab7重组质粒。用脂质体将Rab7真核表达载体瞬时转染RAW264.7细胞,RT-PCR和Western blot法鉴定Rab7的过表达。结果 Rab7重组载体测序结果与GenBank中报道的Rab7序列的同源性为100%。RT-PCR和Western blot结果显示Rab7真核表达载体瞬时转染RAW264.7细胞后,Rab7表达显著增高。结论成功构建了Rab7真核表达载体。为今后研究Rab7在巨噬细胞中的功能奠定了基础。

关键词： Rab7 巨噬细胞真核表达载体过表达