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Visualizing nanopore blinkings in parallel:a high-throughput nanopore array potential for ultra-rapid DNA sequencing

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Science Bulletin 2015年第23期60卷 2067-2068页

作者： Jing-juan xu State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences School of Chemistry and Chemical EngineeringNanjing University

Upon finishing the sequencing of the first complete human genome in 2004[1],the demand for fast DNA reading with less than$1,000 has been fueled by the ever-increasing awareness for personalized medicine and clinical diagnosis[2,3].Plenty of sequencing techniques,from the original Sanger sequencing method to novel sequencing-by-synthesis technologies(Roche,Solexa,SOLi D,etc.)[3],have been developed rapidly in past decades,which are sug-

关键词： DNA测序纳米孔阵列高通量可视化超快速人类基因组测序平行临床诊断

Chemical Measurement and Analysis: from Phenomenon to Essence

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Chinese Journal of Chemistry 2022年第16期40卷 1975-1986页

作者： Wei Zhao Jing-juan xu State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjingJiangsu210023 China Institute of Nanochemistry and Nanobiology School of Environmental and Chemical EngineeringShanghai UniversityShanghai 2004China

The review summaries our achievements in optical,electrochemical,as well as mass spectrometry analysis and imaging with high sensitivity,specificity,and spatiotemporal *** promoted the plasmonic imaging system,and developed ultra-sensitive plasmonic nanoprobes for the tracking of single molecules in single living cells,as well as plasmonic nanocatalysts for highly efficient energy *** addition,we contributed a lot in electrogenerated chemiluminescence(ECL)analysis and *** works including localized surface plasmon resonance(LSPR)enhanced ECL,ECL ratiometric detection,BPE based ECL system,multi-color ECL sensing as well as super-resolved ECL imaging have been initiated by our ***,we also dedicated in nanopipette based electrochemical analysis in single living cells,as well as ESI-MS analysis on short time scale.

关键词： Biosensors Plasmonic imaging Electrogenerated chemiluminescence Electrochemistry Mass spectrometry

Regulation of Ethylene Biosynthesis and Signaling by Protein Kinases and Phosphatases

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Molecular Plant 2014年第6期7卷 939-942页

作者： juan xu Shuqun Zhang State Key Laboratory of Plant Physiology and Biochemistry College of Life Sciences Zhejiang University Hangzhou Zhejiang 310058 China Division of Biochemistry Interdisciplinary Plant Group and Bond Life Sciences Center University of Missouri Columbia MO 65211 USA

Ethylene, a gaseous plant hormone, plays critical roles in plant growth, development, and response to environment. Ethylene-regulated processes are initiated by the elevation of ethylene biosynthesis, which is under tight control by a complex signaling network. An elevated level of ethyl- ene is then perceived by ethylene receptors in local and neighboring cells, which activates signaling pathways that lead to ethylene responses. Different types of tissues/cells have differential capacities in producing ethylene and dif- ferential sensitivity to ethylene, which are crucial to the diverse functions of ethylene in plants. This report high- lights recent advances in our understanding of kinases and phosphatases in ethylene biosynthesis and signaling.

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The constitutions of terpenoids and hormones of leaves in red-flesh citrus mutants and their wild types

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Horticultural Plant Journal 2022年第3期8卷 297-310页

作者： Cuihua Liu Min He juan xu College of Horticulture Northwest A&F UniversityYangling 712100ShaanxiChina Key Laboratory of Horticultural Plant Biology(Ministry of Education) College of Horticulture and ForestryHuazhong Agricultural UniversityWuhan 430070China

To study the profiles and relationships of terpenoids and phytohormones in leaves of red-flesh citrus,we examined four red-flesh mutants that accumulate lycopene in the fruit and their corresponding wild *** compared their contents of monoterpenoid and sesquiterpenoid volatiles,triterpenoid limonoid aglycones,and tetraterpenoid *** types and concentrations of these terpenoids in the leaves varied among genotypes,and the leaf terpenoid profiles differed between red-fleshmutants and theirwild ***,lycopenewas not detected in citrus leaves of all eight varieties,including the *** to phytohormone accumulation in the leaves,the citrus varieties could be classified into a low-phytohormone group(‘Red Anliu’sweet orange,‘Anliu’sweet orange,and‘Cara Cara’navel orange),a high-phytohormone group(‘Seike’navel orange),and a high-jasmonic acid group(‘Red-flesh Guanxi’pummelo,‘Guanxi’pummelo,‘Chuhong’pummelo,and‘Feicui’pummelo).The contents of terpenoid volatiles were very low in leaves of‘Red-flesh Guanxi’and‘Guanxi’pummelo;therefore,they are ideal materials for verifying the functions of genes related to terpenoid volatiles in overexpression *** volatiles were positively correlated with phytohormones of abscisic acid,jasmonic acid,and salicylic *** together,lycopene was undetected in the leaves of redflesh citrus mutants with abundant lycopene in their juice sacs,which suggested a tissue-specific accumulation pattern of lycopene in citrus red-flesh ***,leaf volatile profiles could be suitable to develop fingerprint chromatograms of citrus resources,since leaf volatile profiles were dominated by several compounds that varied among genotypes in their combinations and concentrations.

关键词： Citrus Carotenoid Terpenoid volatile Limonoid aglycone Hormone Leaf

Analysis and Improvement of an Efficient Controlled Quantum Secure Direct Communication and Authentication Protocol

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Computers, Materials & Continua 2018年第12期57卷 621-633页

作者： Jifeng Zhong Zhihao Liu juan xu Navigation College Jimei UniversityXiamen361021China School of Computer Science and Engineering Southeast UniversityNanjing211189China Key Laboratory of Computer Network and Information Integration Southeast UniversityMinistry of EducationNanjing211189China Institute for Quantum Computing University of WaterlooWaterlooONCanadaN2L3G1

The controlled quantum secure direct communication(CQSDC)with authentication protocol based on four particle cluster states via quantum one-time pad and local unitary operations is *** is found that there are some serious security issues in this *** eavesdropper(Eve)can eavesdrop on some information of the identity strings of the receiver and the controller without being detected by the selective-CNOT-operation(SCNO)*** the same attack,Eve can also steal some information of the secret message that the sender *** addition,the receiver can take the same kind of attack to eavesdrop on some information of the secret message out of the control of the *** means that the requirements of CQSDC are not *** last,we improve the original CQSDC protocol to a secure one.

关键词： Quantum cryptography controlled quantum secure direct communication selective-CNOT-operation attack

A Memory-efficient Simulation Method of Grover’s Search Algorithm

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Computers, Materials & Continua 2018年第11期57卷 307-319页

作者： xuwei Tang juan xu Bojia Duan College of Computer Science and Technology Nanjing University of Aeronautics and AstronauticsNanjingChina Institute for Quantum Computing University of WaterlooCanada Key Laboratory of Computer Network and Information Integration(Southeast University) Ministry of EducationChina

Grover’s search algorithm is one of the most significant quantum algorithms,which can obtain quadratic speedup of the extensive search *** Grover's search algorithm cannot be implemented on a real quantum computer at present,its quantum simulation is regarded as an effective method to study the search *** simulating the Grover's algorithm,the storage space required is exponential,which makes it difficult to simulate the high-qubit Grover’s *** this end,we deeply study the storage problem of probability amplitude,which is the core of the Grover simulation *** propose a novel memory-efficient method via amplitudes compression,and validate the effectiveness of the method by theoretical analysis and simulation *** results demonstrate that our compressed simulation search algorithm can help to save nearly 87.5%of the storage space than the uncompressed *** under the same hardware conditions,our method can dramatically reduce the required computing nodes,and at the same time,it can simulate at least 3 qubits more than the uncompressed ***,our memory-efficient simulation method can also be used to simulate other quantum algorithms to effectively reduce the storage costs required in simulation.

关键词： Grover’s search algorithm probability amplitude quantum simulation memory compression

Recent advances in near infrared(NIR)electrochemiluminescence luminophores

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Chinese Chemical Letters 2024年第11期35卷 16-24页

作者： Yuyang Zhou Ziwang Mao Jing-juan xu State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China School of Chemistry and Life Sciences Suzhou University of Science and TechnologySuzhou 215009China

Electrochemiluminescence has been developed as a robust analytical technique owing to its intrinsic advantages,such as near-zero background signal noise,wide dynamic ranges,high sensitivity and low cost and simple *** luminophore as the critical component to generate light signals plays significant roles in this robust analytical *** with traditional ECL luminophores,near infrared(NIR)ECL luminophores have attracted significant attentions recently due to their negligible autofluorescence,lower background interference and deep tissue *** substantial progresses have been achieved in exploring novel NIR ECL luminophores and elucidating their roles in addressing diverse challenges,there is still scarce of comprehensive reviews on the development of NIR ECL luminophores so *** this review,the recent advancements on NIR ECL materials,including inorganic metal complexes,organic small molecules,metal nanoclusters,quantum dots and lanthanide-based materials,have been thoroughly summarized and *** addition,we also provide a comprehensive overview of the challenges and prospects that lie ahead for the future development of NIR ECL luminophores in the future.

关键词： Electrochemiluminescence NIR Luminophore Nanocluster Metal complex

Zinc isotopic behavior of mafic rocks during continental deep subduction

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Geoscience Frontiers 2021年第5期12卷 182-191页

作者： Li-juan xu Sheng-Ao Liu Shuguang Li State Key Laboratory of Geological Processes and Mineral Resources School of Scientific ResearchChina University of GeosciencesBeijing 100083China

Zinc isotopes may act as a new tool of tracking recycling of crustal materials that causes compositional heterogeneity of the *** application relies on an investigation of Zn isotopic behaviors during slab *** this study,we report Zn isotopic compositions for a suite of metabasalts(greenschists,amphibolites,and coesitebearing eclogites)from the Dabie Orogen(China),which were formed via the subduction of mafic rocks into different depths and up to>200 *** out of eight greenschists are characterized by lighterδ^(66)ZnJMC-Lyon(0.10‰-0.16‰)than those of global basalts(0.28‰±0.05‰),which may be caused by crustal assimilation of the protoliths by sedimentary rocks due to their extremely high ^(87)Sr/^(86)Sr(up to 0.7130)and lowεNd values(down to−12.3).The remaining greenschists have relatively low ^(87)Sr/^(86)Sr and theirδ66Zn values(0.21‰-0.38‰)overlap the ranges of amphibolites(0.18‰-0.32‰)and coesite-bearing eclogites(0.18‰-0.36‰).There is no correlation betweenδ^(66)Zn and sensitive indicators of dehydration(Rb/TiO_(2),Ba/Yb,and H_(2)O+),suggesting that no detectable Zn isotope fractionation has occurred during the deep subduction of mafic rocks even into>200 km,which is attributed to the limited loss of Zn during prograde metamorphism and ***,Zn isotopic compositions of the deeply subducted mafic rocks are inherited from their *** that these metamorphosed rocks have higherδ66Zn than that of the mantle value by up to 0.2‰,the recycled/subducted mafic crust can incorporate isotopically heavy Zn into the *** subducted slabs may partially melt and generate a metasomatized mantle,resulting in changes of Zn isotopic composition of the hybridized mantle as have been observed in some mantle xenoliths and basaltic lavas.

关键词： Zn isotopes Metamorphosed rocks Dehydration Crustal deep subduction

Human IgG Fc promotes expression, secretion and immunogenicity of enterovirus 71 VP1 protein

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The Journal of Biomedical Research 2016年第3期30卷 209-216页

作者： juan xu Chunhua Zhang Department of Immunology Nanjing Medical UniversityNanjingJiangsu 210029China Department of Infectious Diseases The First Affiliated HospitalNanjing Medical UniversityNanjingJiangsu 210029China China-US Vaccine Research Center The First Affiliated HospitalNanjing Medical UniversityNanjingJiangsu 210029China

Enterovirus （EV71） can cause severe neurological diseases, but the underlying pathogenesis remains unclear. The capsid protein, viral protein 1 （VP1）, plays a critical role in the pathogenicity of EVT1. High level expression and secretion ofVP 1 protein are necessary for structure, function and immunogenicity in its natural conformation. In our previous studies, 5 codon-optimized VP 1 DNA vaccines, including wt-VP 1, tPA-VP 1, VP l-d, VP 1-hFc and VP 1 - mFc, were constructed and analyzed. They expressed VP1 protein, but the levels of secretion and immunogenicity of these VP1 constructs were significantly different （P〈0.05）. In this study, we further investigated the protein lev- els of these constructs and determined that all of these constructs expressed VP1 protein. The secretion level was increased by including a tPA leader sequence, which was further increased by fusing human IgG Fc （hFc） to VP1. VP 1-hFc demonstrated the most potent immunogenicity in mice. Furthermore, hFc domain could be used to purify VPI-hFc protein for additional studies.

关键词： enterovirus 71 VP1 DNA vaccine human IgG Fc immunogenicity