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Chinese Journal of Structural Chemistry 2020年第4期39卷 793-800页

作者： song juan DUAN Bo-Feng LU Jiu-Fu GE Hong-Guang Shaanxi Key Laboratory of Catalysis College of Chemical&Environment ScienceShaanxi University of TechnologyHanzhong 723001China Mechanical Engineering Training Center Shaanxi University of TechnologyHanzhong 723001China

Three new lanthanide coordination polymers[Ln(bppc)2(suc)0.5(H2O)]n·nH2O(Ln=Eu(1),Ln=Dy(2),Ln=Tb(3),Hbppc=2,6-bis(pyrazin-2-yl)pyridine-4-carboxylate,H2suc=succinic acid)have been hydrothermally synthesized and structurally *** compounds are isostructural and crystallize in triclinic P1 space *** Ln(Ⅲ)ions are eightcoordinated with dodecahedral coordination ***(Ⅲ)cations are linked by carboxylate groups from(bppc)-ligands to form one-dimensional ring chains,which are further connected by the carboxylate groups of(bppc)-and(suc)2-ligands to extend a 2D layer *** coordination polymers display the characteristic emission bands of Ln(Ⅲ)ions in the solid state.

关键词： lanthanide coordination polymers crystal structure luminescence properties

水稻组蛋白H1三突变体的创建和转录组学分析

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生物技术通报 2024年第4期40卷 85-96页

作者：杨淇魏子迪宋娟童堃杨柳王佳涵刘海燕栾维江马轩天津师范大学生命科学学院天津市动植物抗性重点实验室天津300387

【目的】组蛋白H1对于染色质高级结构的维持和稳定具有重要作用,研究水稻H1对基因表达的影响,为深入理解水稻H1的生物学调控功能提供依据。【方法】通过半定量RT-PCR与RT-qPCR对水稻4个H1基因进行表达分析,利用CRISPR技术创建水稻H1基... 详细信息

【目的】组蛋白H1对于染色质高级结构的维持和稳定具有重要作用,研究水稻H1对基因表达的影响,为深入理解水稻H1的生物学调控功能提供依据。【方法】通过半定量RT-PCR与RT-qPCR对水稻4个H1基因进行表达分析,利用CRISPR技术创建水稻H1基因编辑植株,鉴定突变体表型,对突变体进行转录组学分析。【结果】水稻4个H1基因的表达比较广谱,在根中的表达较低;在T_(0)代CRISPR突变体筛选过程中发现H1.1-H1.4发生多种突变;在T_(1)代筛选到一株Osh1.1 Osh1.3 Osh1.4纯合三突变体,该三突变体具有多种发育缺陷,成为转录组分析的材料;进一步在T_(2)代得到三突和四突群体,该群体约25%为白化苗,植株生长迟缓,在响应干旱胁迫方面发生缺陷;对三突变体进行转录组学分析,鉴定到1055个差异表达基因,显著上调的基因约为下调基因的2.5倍,说明H1可能具有抑制基因表达的功能。【结论】在突变体中,光合作用、胁迫响应、氨基酸代谢和RNA代谢等多种途径均发生调控紊乱;其中,核糖体蛋白和光合作用相关基因显著上调,与干旱胁迫相关的脱氢酶基因显著下调。核糖体途径基因过量表达可能造成蛋白质稳态失调,导致植物发育缺陷。

关键词：水稻组蛋白H1 CRISPR 转录组测序 RT-qPCR

Metal-free phthalocyanine single crystal： Solvothermal synthesis and near-infrared electroluminescence

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Chinese Chemical Letters 2016年第5期27卷 764-768页

作者： Qing-Long Bai Chun-Hua Zhang juan-juan song Jing-Hai Liu Yan-Chun Feng Li-Mei Duan Chuan-Hui Cheng Inner Mongolia Key Lab of Chemistry of Natural Products and Synthesis of Functional Molecules College of Chemistry and Chemical Engineering Inner Mongolia University for Nationalities Tongliao 028000 China School of Physics and Optoelectronics Technology Dalian University of Technology Dalian 116024 China

A metal-flee purple H2Pc single crystal was synthesized by a facile solvothermal method, and its solubility and near-infrared （NIR） optical properties were also investigated due to its potential applications as a light-emitting layer for OLEDs. The H2Pc single crystal is insoluble in 1-chlorine naphthalene and other organic solvents. It gives a wide absorption in the range from 620 nm to 679 nm and a wide emission in near 922 nm. As an active light-emitting layer, H2Pc was employed to fabricate electroluminescent （EL） devices with a structure of ITO/NPB （30 nm）/Alq3：H2Pc （30 nm）/BCP （20 nm）/ Alq3 （20 nm）/Al. The emission center is at 936 nm when the H2Pc doping concentration is 20 wt%. The doping concentration strongly governs the emission intensity. When doping concentration decreases from 10 wt% to 1 wt%, the emission intensity remarkably fades, and simultaneously the emission center undergoes a blue shift.

关键词： Solvothermal synthesis H2Pc single crystal Optical properties Near-infrared Electroluminescence

1-磷酸鞘氨醇信号激活对乳腺癌BT549细胞增殖的影响

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中国临床药理学杂志 2024年第11期40卷 1578-1582页

作者：宋娟王明刘欣洋张浩天张奇赵学梅刘颖崔红霞齐齐哈尔医学院药学院黑龙江齐齐哈尔161006

目的研究1-磷酸鞘氨醇(S1P)信号激活对乳腺癌BT549细胞增殖的影响。方法将细胞分为对照组和实验组。实验组用0.1、1.0和10.0μmol·L^(-1)S1P受体激动药SEW2871处理乳腺癌细胞72 h;对照组用含0.1%胎牛血清培养基培养。用噻唑蓝(MTT)法... 详细信息

目的研究1-磷酸鞘氨醇(S1P)信号激活对乳腺癌BT549细胞增殖的影响。方法将细胞分为对照组和实验组。实验组用0.1、1.0和10.0μmol·L^(-1)S1P受体激动药SEW2871处理乳腺癌细胞72 h;对照组用含0.1%胎牛血清培养基培养。用噻唑蓝(MTT)法检测细胞增殖情况。建立乳腺癌BT549细胞过表达S1P受体的细胞模型,将转染的细胞分为空白质粒组(LUC)、野生型S1P受体组(WT)、S1P受体磷酸化位点突变组(MUT)。用MTT法检测细胞增殖情况,并计算细胞增殖率;用克隆集落形成实验检测细胞克隆形成能力。用S1P受体拮抗药W146(10μmol·L^(-1))及蛋白激酶B(AKT)信号通路抑制药MK2206(90 nmol·L^(-1))检测S1P信号在乳腺癌BT549细胞增殖中的作用,用蛋白质印迹法检测磷酸化信号传导与转录激活因子-3(p-STAT3)蛋白、原癌基因c-Myc蛋白的表达情况。结果对照组和0.1、1.0和10.0μmol·L^(-1)实验组的细胞增殖率分别为1.00±0.03、1.13±0.06、1.06±0.10和1.07±0.03,SEW2871在0.1μmol·L^(-1)时可促进细胞增殖(P<0.05)。过表达S1P受体后,WT组、MUT组与LUC组相比,细胞增殖率和克隆集落形成数量均明显增加(均P<0.05)。用W146处理后,LUC组、MUT组、WT组的细胞相对增殖率分别为1.25±0.12、1.31±0.03和1.43±0.14;用MK2206处理后LUC组、MUT组、WT组的细胞相对增殖率分别为0.87±0.15、0.77±0.03和0.88±0.02;WT组、MUT组与相应DSMO组比较,在统计学上差异均有统计学意义(均P<0.01)。用W146处理后,LUC组、MUT组、WT组的细胞克隆形成数量分别为(65.65±5.12),(141.48±5.63)和(93.64±5.14)个,WT组、MUT组与相应DSMO组比较,在统计学上差异均有统计学意义(均P<0.05)。MK2206处理后,LUC组、MUT组、WT组的p-STAT3蛋白相对表达水平分别为0.67±0.04、0.69±0.08和0.81±0.06,原癌基因c-Myc蛋白相对表达水平分别为1.69±0.03、0.70±0.10和0.67±0.07,WT组、MUT组的上述指标与DMSO组比较,在统计学上差异均有统计学意义(均P<0.05)。结论S1P信号激活可促进乳腺癌BT549细胞增殖,其机制可能与AKT及STAT3信号通路有关。

关键词： 1-磷酸鞘氨醇受体激动药和拮抗药蛋白激酶B信号通路抑制药 1-磷酸鞘氨醇信号乳腺癌细胞增殖

Green manuring facilitates bacterial community dispersal across different compartments of subsequent tobacco

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Journal of Integrative Agriculture 2023年第4期22卷 1199-1215页

作者： LIANG Hai FU Li-bo CHEN Hua ZHOU Guo-peng GAO song-juan CAO Wei-dong College of Resources and Environmental Sciences Nanjing Agricultural UniversityNanjing 210095P.R.China Institute of Agricultural Environment and Resources Yunnan Academy of Agricultural SciencesKunming 650205P.R.China Key Laboratory of Plant Nutrition and Fertilizer Ministry of Agriculture and Rural Affairs/Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081P.R.China

Green manure–crop rotation is a sustainable approach to protect crops against diseases and improve ***,the mechanism by which green manuring manipulates the crop-associated microbial community remains to be *** this study,we explored the horizontal processes of bacterial communities in different compartments of the soil–root interface(bulk soil,rhizosphere soil,rhizoplane and endosphere)of tobacco by performing a field experiment including four rotation practices,namely,tobacco rotated with smooth vetch,ryegrass,radish,and winter fallow(without green manure).Results showed that the co-occurrence networks constructed by adjacent compartments of the soil–root interface with green manuring had more edges than without green manuring,indicating dramatic microbial *** manuring increased the dispersal-niche continuum index between bulk soil and other compartments,indicating that it facilitated the horizontal dispersal of *** the different green manuring practices,the neutral community model explained 24.6–27.6%of detection frequency for bacteria,and at least one compartment under each practice had a normalized stochasticity ratio higher than the 50%boundary point,suggesting that the deterministic and stochastic processes jointly shaped the tobacco *** conclusion,green manuring generally facilitates bacterial community dispersal across different compartments and enhances potential interactions among adjacent *** study provides empirical evidence for understanding the microbiome assembly under green manure–crop rotation.

关键词： green manure tobacco rotation practice niche compartment community assembly

Arbuscular mycorrhizal fungi communities associated with wild plants in a coastal ecosystem

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Journal of Forestry Research 2021年第2期32卷 683-695页

作者： Jinping Wang Shilin Ma G.Geoff Wang Linhao Xu Zhiyuan Fu juan song Jinchi Zhang Co-Innovation Center for Sustainable Forestry in Southern China Jiangsu Province Key Laboratory of Soil and Water Conservation and Ecological RestorationNanjing Forestry University159 Longpan RoadNanjing 210037JiangsuChina Department of Forestry and Environmental Conservation Clemson UniversityClemsonSC 29634USA

Arbuscular mycorrhizal fungi(AMF)form a near-ubiquitous mutualistic association with roots to help plants withstand harsh environments,and play a key role in the establishment of coastal beach plant *** little is known about the structure and composition of AMF communities on coastal beaches of eastern *** this study,we investigated the occurrence,community composition and diversity of AMF associated with common wild plants on a coastal beach of North Jiangsu,*** all of the local wild species were colonized by AMF except for Chenopodium album ***-seven AMF species were isolated from the rhizosphere belonging to 12 genera in seven *** was the dominant genus and Funneliformis mosseae the dominant *** colonization,spore composition and diversity of AMF were strongly related to edaphic ***(Na^(+))ions in the soil significantly and negatively affected the colonization rate by AMF and both soil Na^(+)levels and pH had a significant negative effect on AMF spore density and ***,there was a significant positive correlation between species richness and total organic *** results provide insights into soil factors affecting native AMF communities in coastal beach habitats which could benefit vegetation recovery and soil reclamation efforts.

关键词： AMF diversity Coastal beach Edaphic factors Mycorrhizal composition Wild hosts

Astrocytes: a double-edged sword in neurodegenerative diseases

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Neural Regeneration Research 2021年第9期16卷 1702-1710页

作者： Zhi-Bin Ding Li-juan song Qing Wang Gajendra Kumar Yu-Qing Yan Cun-Gen Ma The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine Research Center of NeurobiologyShanxi University of Chinese MedicineTaiyuanShanxi ProvinceChina Institute of Brain Science Shanxi Key Laboratory of Inflammatory Neurodegenerative DiseasesMedical School of Shanxi Datong UniversityDatongShanxi ProvinceChina Department of Neurology Affiliated Shanxi Bethune HospitalShanxi Medical UniversityTaiyuanShanxi ProvinceChina Department of Neuroscience City University of Hong KongTat Chee AvenueHong Kong Special Administrative RegionChina

Astrocytes play multifaceted and vital roles in maintaining neurophysiological function of the central nervous system by regulating homeostasis, increasing synaptic plasticity, and sustaining neuroprotective effects. Astrocytes become activated as a result of inflammatory responses during the progression of pathological changes associated with neurodegenerative disorders. Reactive astrocytes(neurotoxic A1 and neuroprotective A2) are triggered during disease progression and pathogenesis due to neuroinflammation and ischemia. However, only a limited body of literature describes morphological and functional changes of astrocytes during the progression of neurodegenerative diseases. The present review investigated the detrimental and beneficial roles of astrocytes in neurodegenerative diseases reported in recent studies, as these cells have promising therapeutic potential and offer new approaches for treatment of neurodegenerative diseases.

关键词： A1 A2 astrocytes neurodegenerative diseases neuroinflammation neuron neuroprotection neurotoxicity polarization reactivity

基于CT扫描的左心房结构参数与心房颤动患者射频消融术后1年内复发的关系研究

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实用心脑肺血管病杂志 2024年第6期32卷 26-29页

作者：沈金链宋娟王睿严鹏勇罗章源上海中医药大学附属普陀医院急诊科上海市200062 上海中医药大学附属普陀医院心内科上海市200062 恩识医疗科技(上海)有限公司上海市201112

目的探究基于CT扫描的左心房结构参数与心房颤动(AF)患者射频消融术(RFA)后1年内复发的关系。方法选取2020年8月—2022年8月在上海中医药大学附属普陀医院急诊科接受RFA的AF患者196例为研究对象,根据患者RFA后1年内有无复发将其分为复发... 详细信息

目的探究基于CT扫描的左心房结构参数与心房颤动(AF)患者射频消融术(RFA)后1年内复发的关系。方法选取2020年8月—2022年8月在上海中医药大学附属普陀医院急诊科接受RFA的AF患者196例为研究对象,根据患者RFA后1年内有无复发将其分为复发组(n=51)和未复发组(n=145)。收集患者的一般资料及术前基于CT扫描的左心房结构参数、实验室检查指标。采用多因素Logistic回归分析、ROC曲线探讨基于CT扫描的左心房结构参数与AF患者RFA后1年内复发的关系。结果复发组持续性AF占比高于未复发组,左心房左外侧脊(LLR)长度、左心房直径(LAD)长于未复发组,左心房容积指数(LAVI)高于未复发组,左心耳体积大于未复发组(P<0.05)。多因素Logistic回归分析结果显示,左心房LLR长度、LAVI、左心耳体积是AF患者RFA后1年内复发的独立影响因素(P<0.05)。ROC曲线分析结果显示,左心房LLR长度、LAVI、左心耳体积预测AF患者RFA后1年内复发的AUC分别为0.901〔95%CI(0.855~0.947)〕、0.897〔95%CI(0.855~0.940)〕、0.972〔95%CI(0.945~0.999)〕。结论左心房LLR长度、LAVI、左心耳体积是AF患者RFA后1年内复发的影响因素,且三者对AF患者RFA后1年内复发具有较高/中等的预测价值。

关键词：心房颤动射频消融术左心房复发

E2E-MFERC:AMulti-Face Expression Recognition Model for Group Emotion Assessment

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Computers, Materials & Continua 2024年第4期79卷 1105-1135页

作者： Lin Wang juan Zhao Hu song Xiaolong Xu Jiangsu Key Laboratory of Big Data Security&Intelligent Processing Nanjing University of Posts and TelecommunicationsNanjing210042China School of Network Security Jinling Institute of TechnologyNanjing211169China State Grid Jiangsu Electric Power Company Limited Nanjing210000China School of Computer Science Nanjing University of Posts and TelecommunicationsNanjing210042China

In smart classrooms, conducting multi-face expression recognition based on existing hardware devices to assessstudents’ group emotions can provide educators with more comprehensive and intuitive classroom effect analysis,thereby continuouslypromotingthe improvementof teaching ***,most existingmulti-face expressionrecognition methods adopt a multi-stage approach, with an overall complex process, poor real-time performance,and insufficient generalization ability. In addition, the existing facial expression datasets are mostly single faceimages, which are of low quality and lack specificity, also restricting the development of this research. This paperaims to propose an end-to-end high-performance multi-face expression recognition algorithm model suitable forsmart classrooms, construct a high-quality multi-face expression dataset to support algorithm research, and applythe model to group emotion assessment to expand its application value. To this end, we propose an end-to-endmulti-face expression recognition algorithm model for smart classrooms (E2E-MFERC). In order to provide highqualityand highly targeted data support for model research, we constructed a multi-face expression dataset inreal classrooms (MFED), containing 2,385 images and a total of 18,712 expression labels, collected from smartclassrooms. In constructing E2E-MFERC, by introducing Re-parameterization visual geometry group (RepVGG)block and symmetric positive definite convolution (SPD-Conv) modules to enhance representational capability;combined with the cross stage partial network fusion module optimized by attention mechanism (C2f_Attention),it strengthens the ability to extract key information;adopts asymptotic feature pyramid network (AFPN) featurefusion tailored to classroomscenes and optimizes the head prediction output size;achieves high-performance endto-end multi-face expression detection. Finally, we apply the model to smart classroom group emotion assessmentand provide design refe

关键词： Multi-face expression recognition smart classroom end-to-end detection group emotion assessment

Viral Contamination Source in Clinical Microbiology Laboratory

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Biomedical and Environmental Sciences 2016年第8期29卷 609-611页

作者： WANG Xin Ling song juan song Qin Qin YU Jie LUO Xiao Nuan WU Gui Zhen HAN Jun State Key Laboratory for Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention

To understand the potential causes of laboratory-acquired infections and to provide possible solutions that would protect laboratory personnel, samples from a viral laboratory were screened to determine the main sources of contamination with six subtypes of Rhinovirus. Rhinovirus contamination was found in the gloves, cuffs of protective wear, inner surface of biological safety cabinet （BSC） windows, and trash handles. Remarkably, high contamination was found on the inner walls of the centrifuge and the inner surface of centrifuge tube casing in the rotor. Spilling infectious medium on the surface of centrifuge tubes was found to contribute to contamination of centrifuge surfaces. Exposure to sodium hypochlorite containing no less than 0.2 g/L available chlorine decontaminated the surface of the centrifuRe tubes from Rhinovirus after 2 min.

关键词： Viral Contamination Source in Clinical Microbiology Laboratory BSC HRV