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脑源性神经营养因子在谷氨酸钠诱导的中枢性肥胖小鼠海马组织中表达的研究

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中国临床药理学杂志 2024年第15期40卷 2227-2230页

作者：曹鹏娟唐嘉媛杨美子李园园黄立婷蒙雯雯金勇君莫建勋中国人民解放军联勤保障部队第九二四医院内分泌科广西壮族自治区桂林541000 滨州医学院药理学教研室山东烟台264000 滨州医学院烟台附属医院内分泌科山东烟台264000

目的探讨中枢性肥胖小鼠是否通过影响海马组织中脑源性神经营养因子(BDNF)的表达引起认知功能的下降。方法自小鼠出生当天起,按体质量随机分为正常组和模型组。模型组按3 mg·g^(-1)·d^(-1)的剂量颈部皮下注射10%L-谷氨酸钠(MSG),连续5... 详细信息

目的探讨中枢性肥胖小鼠是否通过影响海马组织中脑源性神经营养因子(BDNF)的表达引起认知功能的下降。方法自小鼠出生当天起,按体质量随机分为正常组和模型组。模型组按3 mg·g^(-1)·d^(-1)的剂量颈部皮下注射10%L-谷氨酸钠(MSG),连续5 d;正常组同法注射等剂量0.9%NaCl。最终正常组和模型组各入选8只。比较2组小鼠体质量、Lee’s指数和血脂变化,用蛋白质印迹法检测2组小鼠海马组织中BDNF蛋白的表达水平。结果8周末,模型组和正常组的体质量分别为(49.01±2.47)和(41.27±3.28)g,Lee’s指数分别为(357.14±9.24)和(330.15±7.37)g^(1/3)·cm^(-1),三酰甘油分别为(1.37±0.52)和(0.73±0.31)mmol·L^(-1),总胆固醇分别为(2.98±0.18)和(1.98±0.30)mmol·L^(-1),低密度脂蛋白分别为(0.31±0.03)和(0.24±0.02)mmol·L^(-1),高密度脂蛋白分别为(2.70±0.15)和(1.98±0.40)mmol·L^(-1),在统计学上差异均有统计学意义(P<0.05,P<0.01),符合中枢性肥胖模型特点。模型组和正常组海马组织中BDNF蛋白相对表达水平分别为6.02×10^(4)±626.53和7.04×10^(4)±1440.81,在统计学上差异有统计学意义(P<0.01)。结论中枢性肥胖小鼠可能通过下调海马组织中BDNF的表达来引起认知功能的下降。

关键词：脑源性神经营养因子谷氨酸钠中枢性肥胖海马认知功能

Arabidopsis F-box gene FOA1 involved in ABA signaling

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Science China(Life Sciences) 2012年第6期55卷 497-506页

作者： peng juan YU DaShi WANG LiQun XIE MinMin YUAN CongYing WANG Yu TANG DongYing ZHAO XiaoYing LIU XuanMing College of Life Sciences State Key Laboratory of Chemo/Biosensing and ChemometricsHunan UniversityChangsha 410082China

The expression of FOAl （F-box overexpressed/oppressed ABA signaling） in different organs of Arabidopsis, and in response to ABA and NaCI, was analyzed. The expression level of FOAl is higher in the root and is lower in the stem, and is induced rapidly by ABA and NaC1. The phenotypes of T-DNA insertion mutant foal and FOAl overexpression lines FOAloxl and FOAlox2 were analyzed. The foal mutant exhibited a lower germination rate, shorter root length, more stomatal opening, in- creased proline accumulation and hypersensitivity to ABA compared with the wild type. In contrast, the overexpression lines showed lower sensitivity to ABA than the wild type. The expression levels of several ABA and stress-responsive transcription factors and genes were altered in the foal mutant in response to ABA. Compared with the wild type, the expression levels of ABA-responsive transcription factors were higher, but ABA and stress-responsive genes were lower in foal mutant. This study demonstrates that FOAl is an ABA signaling-related gene, and may play a negative role in ABA signaling.

关键词： F-box gene FOAl ABA signaling Arabidopsis

基于HPLC-Q-TOF-MS/MS技术的半夏姜制前后成分分析

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时珍国医国药 2024年第8期35卷 1899-1903页

作者：张海鸣张俊华范斌袁芳淼孙健蒋研风羊彭娟梁小雯中国中医科学院医学实验中心广州白云山光华制药股份有限公司

目的采用高效液相色谱-四极杆飞行时间质谱法(HPLC-Q-TOF-MS/MS)对半夏及姜半夏的成分进行鉴定，并比较姜制前后化学成分差异。方法采用HPLC-Q-TOF-MS/MS技术，对半夏和姜半夏的成分进行检测。将采集得到的样品数据导入质谱数据分析软... 详细信息

目的采用高效液相色谱-四极杆飞行时间质谱法(HPLC-Q-TOF-MS/MS)对半夏及姜半夏的成分进行鉴定，并比较姜制前后化学成分差异。方法采用HPLC-Q-TOF-MS/MS技术，对半夏和姜半夏的成分进行检测。将采集得到的样品数据导入质谱数据分析软件Agilent MassHunter Qualitative Analysis 10.0,对各个质谱峰进行提取。搜索自建的数据库，初步鉴定化学成分。再结合文献中化合物、对照品以及MassBank数据库中相关成分的碎片离子信息和保留时间，进行成分确定。此外，将半夏和姜半夏的质谱数据导入安捷伦Profinder 10.0软件，对数据进行峰提取、对齐、归一化等处理。利用Simca 14.1软件对数据进行主成分分析(PCA)及正交偏最小二乘法判别分析(OPLS-DA)。结果在半夏及姜半夏中共鉴定得到43个成分，其中生物碱类成分5个，脂肪酸甘油酯类成分7个，溶血磷脂胆碱类成分7个，醇胺类成分5个，氨基酸类成分7个，酰胺类成分5个，有机酸类1个，姜辣素类2个，其他类成分4个。PCA分析结果显示，半夏姜制前后化学成分具有较大差异。OPLS-DA结果表明，通过VIP值>1,|P(corr)|>0.5筛选得到腺苷、鸟苷、丝胶树碱等18个主要差异成分。结论半夏炮制前后，部分生物碱类、酰胺类、姜辣素类等成分存在明显差异，这些差异性化合物可能导致了半夏姜制前后功效的侧重不同，为提升半夏及其姜制品的质量标准提供参考。

关键词：半夏姜半夏高效液相色谱-四极杆飞行时间质谱主成分分析正交偏最小二乘法判别分析

Effects of processing parameters on fabrication defects,microstructure and mechanical properties of additive manufactured Mg–Nd–Zn–Zr alloy by selective laser melting process

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Journal of Magnesium and Alloys 2024年第6期12卷 2249-2266页

作者： Wenyu Xu penghuai Fu Nanqing Wang Lei Yang Liming peng juan Chen Wenjiang Ding National Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composite School of Materials Science and EngineeringShanghai Jiao Tong University800 Dongchuan RoadShanghai 200240China Shanghai Light Alloy Net Forming National Engineering Research Center Co. LtdShanghai 201615China

Mg–3Nd–0.2Zn–0.4Zr(NZ30K,wt.%)alloy is a new kind of high-performance metallic *** combination of the NZ30K Magnesium(Mg)alloy and selective laser melting(SLM)process seems to be an ideal solution to produce porous Mg degradable ***,the microstructure evolution and mechanical properties of the SLMed NZ30K Mg alloy were not yet studied ***,the fabrication defects,microstructure,and mechanical properties of the SLMed NZ30K alloy under different processing parameters were *** results show that there are two types of fabrication defects in the SLMed NZ30K alloy,gas pores and unfused *** the increase of the laser energy density,the porosity sharply decreases to the minimum first and then slightly *** minimum porosity is 0.49±0.18%.While the microstructure varies from the large grains with lamellar structure inside under low laser energy density,to the large grains with lamellar structure inside&the equiaxed grains&the columnar grains under middle laser energy density,and further to the fine equiaxed grains&the columnar grains under high laser energy *** lamellar structure in the large grain is a newly observed microstructure for the NZ30K Mg *** laser energy density leads to finer grains,which enhance all the yield strength(YS),ultimate tensile strength(UTS)and elongation,and the best comprehensive mechanical properties obtained are YS of 266±2.1 MPa,UTS of 296±5.2 MPa,with an elongation of 4.9±0.68%.The SLMed NZ30K Mg alloy with a bimodal-grained structure consisting of fine equiaxed grains and coarser columnar grains has better elongation and a yield drop phenomenon.

关键词： Selective laser melting Mg alloy Processing parameter Lamellar structure Bimodal-grained structure

Knockouts of RecA-Like Proteins RadC1 and RadC2 Have Distinct Responses to DNA Damage Agents in Sulfolobus islandicus

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Journal of Genetics and Genomics 2013年第10期40卷 533-542页

作者： peng-juan Liang Wen-Yuan Han Qi-Hong Huang Yan-Ze Li Jin-Feng Ni Qun-Xin She Yu-Long Shen State Key Laboratory of Microbial Technology Shandong University Archaeal Centre Department of BiologyUniversity of CopenhagenOle Maal es Vej 5Copenhagen BiocenterDK-2200 CopenhagenDenmark

RecA family recombinases play essential roles in maintaining genome integrity. A group of RecA-like proteins named RadC are present in all archaea, but their in vivo functions remain unclear. In this study, we performed phylogenetic and genetic analysis of two RadC proteins from Sulfolobus islandicus. RadC is closer to the KaiC lineage of cyanobacteria and proteobacteria than to the lineage of the recombinases （RecA, RadA, and Rad51） and the recombinase paralogs （e.g., RadB, Rad55, and Rad51B）. Using the recently- established S. islandicus genetic system, we constructed deletion and over-expression strains of radC1 and radC2. Deletion of radC1 rendered the cells more sensitive to DNA damaging agents, methyl methanesulfonate （MMS）, hydroxyurea （HU）, and ultraviolet （UV） radiation, than the wild type, and a AradCIAradC2 double deletion strain was more sensitive to cisplatin and MMS than the AradC1 single deletion mutant. In addition, ectopic expression of His-tagged RadC 1 revealed that RadC I was co-purified with a putative structure-specific nuclease and ATPase, which is highly conserved in archaea. Our results indicate that both RadCI and RadC2 are involved in DNA repair. RadCl may play a general or primary role in DNA repair, while RadC2 plays a role in DNA repair in response to specific DNA damages.

关键词： RecA-like protein RadA RadC Sulfolobus DNA repair

肾移植术后新发泌尿系统恶性肿瘤二例并文献复习

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中华移植杂志（电子版） 2024年第1期18卷 35-39页

作者：赵丽蔡瑞明赵纪强林民专陈志勇彭娟广州医科大学附属第三医院病理科、广东省产科重大疾病重点实验室、广东省妇产疾病临床医学研究中心、粤港澳母胎医学高校联合实验室 510150 广州医科大学附属第三医院器官移植科 510150

随着免疫抑制剂的发展,肿瘤已取代排斥反应等其他肾移植术后并发症成为导致受者术后死亡的主要原因之一[1]。与普通人群相比,实体器官移植受者罹患恶性肿瘤的风险增加2~4倍,且预后更差[2]。许多危险因素与移植后恶性肿瘤发病率升高有关... 详细信息

随着免疫抑制剂的发展,肿瘤已取代排斥反应等其他肾移植术后并发症成为导致受者术后死亡的主要原因之一[1]。与普通人群相比,实体器官移植受者罹患恶性肿瘤的风险增加2~4倍,且预后更差[2]。许多危险因素与移植后恶性肿瘤发病率升高有关,包括免疫抑制类型、强度和持续时间以及致癌性病毒感染和阳光照射。有数据显示,肾移植术后免疫抑制治疗时间>10年者,其术后恶性肿瘤发生率达20%,超过心血管疾病,成为影响肾移植受者长期存活的重要因素[1,2,3]。本文回顾性分析广州医科大学附属第三医院2例肾移植术后新发泌尿系统恶性肿瘤受者临床资料,并结合文献对近年来肾移植受者术后并发恶性肿瘤的病理特征、临床诊治及预后进行分析,旨在提高诊治水平和改善受者预后,现报道如下。

关键词：肾移植肾细胞癌尿路上皮癌

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四川省内江市部分种猪场伪狂犬病检测分析

畜禽业 2024年第8期35卷 80-83页

作者：张其彬雷明霞徐凯彭娟蒋凛余姣李梅内江市畜牧业发展中心四川内江641000 内江市动植物疫病防控和农产品质量检测中心四川内江641000

为了解四川省内江市种猪场伪狂犬病毒野毒感染和免疫状况,以针对性地开展猪伪狂犬病的防控与净化。在全市9个种猪场采集血清样本438份、口鼻拭子142份、公猪精液105份、流产胎儿组织32份,采用ELISA方法和荧光PCR方法,分别进行了猪伪狂... 详细信息

为了解四川省内江市种猪场伪狂犬病毒野毒感染和免疫状况,以针对性地开展猪伪狂犬病的防控与净化。在全市9个种猪场采集血清样本438份、口鼻拭子142份、公猪精液105份、流产胎儿组织32份,采用ELISA方法和荧光PCR方法,分别进行了猪伪狂犬病毒gB蛋白、gE蛋白抗体检测和gE基因病原检测。结果显示:在9个场点采集的438份猪血清中,猪伪狂犬病病毒gB蛋白抗体检出阳性率为89.5%,gE蛋白抗体检出个体阳性率为7.08%、场户阳性率11.11%;在717份病原学样品中,检出阳性15份,个体阳性率2.09%,场户阳性率22.22%;整体抗体水平较高,但不同场点、不同阶段抗体存在一定差异,个别场群存在免疫抗体水平低下情况,野毒感染有逐渐上升趋势。因此,应重视种猪群猪伪狂犬病防控,不断强化免疫和监测,加强疫病防控,有序推进净化。

关键词：猪伪狂犬病 gB抗体 gE抗体种猪阳性率

A Novel Graph Structure Learning Based Semi-Supervised Framework for Anomaly Identification in Fluctuating IoT Environment

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Computer Modeling in Engineering & Sciences 2024年第9期140卷 3001-3016页

作者： Weijian Song Xi Li peng Chen juan Chen Jianhua Ren Yunni Xia School of Computer and Software Engineering Xihua UniversityChengdu610039China West China Second University Hospital Sichuan UniversityChengdu610065China School of Computer Science Chongqing UniversityChongqing400044China

With the rapid development of Internet of Things(IoT)technology,IoT systems have been widely applied in health-care,transportation,home,and other ***,with the continuous expansion of the scale and increasing complexity of IoT systems,the stability and security issues of IoT systems have become increasingly ***,it is crucial to detect anomalies in the collected IoT time series from various ***,deep learning models have been leveraged for IoT anomaly ***,owing to the challenges associated with data labeling,most IoT anomaly detection methods resort to unsupervised learning ***,the absence of accurate abnormal information in unsupervised learning methods limits their *** address these problems,we propose AS-GCN-MTM,an adaptive structural Graph Convolutional Networks(GCN)-based framework using a mean-teacher mechanism(AS-GCN-MTM)for anomaly *** performs better than unsupervised methods using only a small amount of labeled *** Teachers is an effective semi-supervised learning method that utilizes unlabeled data for training to improve the generalization ability and performance of the ***,the dependencies between data are often unknown in time series *** solve this problem,we designed a graph structure adaptive learning layer based on neural networks,which can automatically learn the graph structure from time series *** not only better captures the relationships between nodes but also enhances the model’s performance by augmenting key *** have demonstrated that our method improves the baseline model with the highest F1 value by 10.4%,36.1%,and 5.6%,respectively,on three real datasets with a 10%data labeling rate.

关键词： IoT multivariate time series anomaly detection graph learning semi-supervised mean teachers

Determination of Residues and Safety Analysis of Metal Impurities in Platinum-based Antitumor Drugs

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贵金属 2012年第A1期33卷 258-262页

作者： peng juan PU Shaoping LI Guangli HE Jian ZHANG Zhirong LIAO Yunxing Guiyan Phamaceuitical Co Ltd.Kunming 650106China Kunming University of Science and Technology Kunming 650093China Sino-Platinum Metals Co.Ltd. Kunming 650106China

The residual metal impurities in cisplatin, carboplatin and oxaliplatin were determined by ICP-AES. The samples were ignited and dissolved with HCl:HNO 3 (3:1). The method is simple and accurate. By the determination of the metal residues in the samples, the calculated actual daily exposure and concentration of the metal Pd, Ir, Rh, Ru, Mo, Ni, Cr, V, Cu, Mn, Fe and Zn that were less than the permitted daily exposures (PDE) and the limited concentration permitted in the EMEA guideline on the specification limits for residues of metal catalysts or metal reagents [1] . The metal residues can de adequately removed from the active pharmaceutical ingredients and the corresponding drugs. The trace metal residues will not affect human health and lead to the safety hazard by the intravenous injection.

关键词： ICP-AES platinum-based antitumor drug metal reagent residue safety