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Superior antibody and membrane protein‑specific T‑cell responses to CoronaVac by intradermal versus intramuscular routes in adolescents

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World Journal of Pediatrics 2024年第4期20卷 353-370页

作者： Jaime S.Rosa Duque Samuel M.S.Cheng Carolyn A.Cohen Daniel Leung Xiwei Wang Xiaofeng Mu Yuet Chung Tsun Ming Lau Manni Wang Wenyue Zhang Yanmei Zhang Howard H.W.Wong Leo C.H.Tsang Sara Chaothai Tsz Chun Kwan John K.C.Li Karl C.K.Chan Leo L.H.Luk Jenson C.H.Ho Wing Yan Li Amos M.T.Lee Jennifer H.Y.Lam Sau Man Chan Wilfred H.S.Wong Issan Y.S.Tam Masashi Mori Sophie A.Valkenburg malik peiris Wenwei Tu Yu Lung Lau Department of Paediatrics and Adolescent Medicine The University of Hong KongHong KongChina School of Public Health The University of Hong KongHong KongChina HKU‑Pasteur Research Pole School of Public HealthThe University of Hong KongHong KongChina Research Institute for Bioresources and Biotechnology Ishikawa Prefectural UniversityNonoichiJapan Department of Microbiology and Immunology Peter Doherty Institute for Infectionand ImmunityUniversity of MelbourneMelbourneVICAustralia Center for Immunology and Infection C2i Hong KongChina

Background Optimising the immunogenicity of COVID-19 vaccines to improve their protection against disease is *** dosing by intradermal(ID)administration has been shown to be equally immunogenic as intramuscular(IM)administration for several vaccines,but the immunogenicity of ID inactivated whole severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)at the full dose is *** study(NCT04800133)investigated the superiority of antibody and T-cell responses of full-dose CoronaVac by ID over IM administration in *** Participants aged 11–17 years received two doses of IM or ID vaccine,followed by the 3rd dose 13–42 days *** and cellular immunogenicity outcomes were measured post-dose 2(IM-CC versus ID-CC)and post-dose 3(IMCCC versus ID-CCC).Doses 2 and 3 were administered to 173 and 104 adolescents,*** Spike protein(S)immunoglobulin G(IgG),S-receptor-binding domain(RBD)IgG,S IgG Fcγreceptor IIIa(FcγRIIIa)-binding,SNM[sum of individual(S),nucleocapsid protein(N),and membrane protein(M)peptide pool]-specific interleukin-2(IL-2)+CD4^(+),SNM-specific IL-2^(+)CD8^(+),S-specific IL-2^(+)CD8^(+),N-specific IL-2^(+)CD4^(+),N-specific IL-2^(+)CD8^(+)and M-specific IL-2^(+)CD4^(+)responses fulfilled the superior and non-inferior criteria for ID-CC compared to IM-CC,whereas IgG avidity was *** ID-CCC,S-RBD IgG,surrogate virus neutralisation test,90%plaque reduction neutralisation titre(PRNT90),PRNT50,S IgG avidity,S IgG FcγRIIIa-binding,M-specific IL-2^(+)CD4^(+),interferon-γ+CD8^(+)and IL-2^(+)CD8^(+)responses were superior and non-inferior to *** estimated vaccine efficacies were 49%,52%,66%and 79%for IM-CC,ID-CC,IM-CCC and ID-CCC,*** ID groups reported more local,mild adverse *** This is the first study to demonstrate superior antibody and M-specific T-cell responses by ID inactivated SARSCoV-2 vaccination and serves as the basis for future research to improve the

关键词： CoronaVac Coronavirus disease 2019 Immunity Intradermal Vaccine

污水监测追踪新冠病毒的时空动态变化（英文）

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Engineering 2024年第9期 70-77页

作者：郑夏婉 Kathy Leung 徐晓庆邓妤张玉琳陈熙 Chung In Yau Kenny W.K.Hui Eddie Pak Ho-Kwong Chui 杨绒 Hein Min Tun Gabriel Matthew Leung Joseph Tsz Kei Wu malik peiris Leo Lit Man Poon 张彤 Environmental Microbiome Engineering and Biotechnology Lab Center for Environmental Engineering Research Department of Civil Engineering The University of Hong Kong WHO Collaborating Centre for Infectious Disease Epidemiology and Control School of Public Health Li Ka Shing Faculty of Medicine The University of Hong Kong Laboratory of Data Discovery for Health Litd.(D24H) The University of Hong Kong-Shenzhen Hospital School of Public Health Li Ka Shing Faculty of Medicine The University of Hong Kong Drainage Services Department The Government of the Hong Kong Special Administrative Region Environmental Protection Department The Government of the Hong Kong Special Administrative Region The Jockey Club School of Public Health and Primary Care Faculty of Medicine The University of Hong Kong HKU-Pasteur Research Pole The University of Hong Kong

Wastewater surveillance（WWS） can leverage its wide coverage, population-based sampling, and high monitoring frequency to capture citywide pandemic trends independent of clinical surveillance. Here we conducted a nine months daily WWS for severe acute respiratory syndrome coronavirus 2（SARS-CoV-2） from 12 wastewater treatment plants（WWTPs）, covering approximately 80% of the population,to monitor infection dynamics in Hong Kong, China. We found that the SARS-CoV-2 virus concentration in wastewater was correlated with the daily number of reported cases and reached two pandemic peaks three days earlier during the study period. In addition, two different methods were established to estimate the prevalence/incidence rates from wastewater measurements. The estimated results from wastewater were consistent with findings from two independent citywide clinical surveillance programmes(rapid antigen test（RAT） surveillance and serology surveillance), but higher than the cases number reported by the Centre for Health Protection（CHP） of Hong Kong, China. Moreover, the effective reproductive number（R_t） was estimated from wastewater measurements to reflect both citywide and regional transmission dynamics. Our findings demonstrate that large-scale intensive WWS from WWTPs provides cost-effective and timely public health information, especially when the clinical surveillance is inadequate and costly. This approach also provides insights into pandemic dynamics at higher spatiotemporal resolutions, facilitating the formulation of effective control policies and targeted resource allocation.

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Conservation of T cell epitopes between seasonal influenza viruses and the novel influenza A H7N9 virus

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Virologica Sinica 2014年第3期29卷 170-175页

作者： Huawei Mao Hui-Ling Yen Yinping Liu Yu-Lung Lau J.S.malik peiris Wenwei Tu Department of Paediatrics The University of Hong Kong Shenzhen Hospital Department of Paediatrics & Adolescent Medicine The University of Hong Kong Centre of Influenza Research School of Public Health The University of Hong Kong

A novel avian influenza A(H7N9) virus recently emerged in the Yangtze River delta and caused diseases, often severe, in over 130 people. This H7N9 virus appeared to infect humans with greater ease than previous avian influenza virus subtypes such as H5N1 and H9N2. While there are other potential explanations for this large number of human infections with an avian influenza virus, we investigated whether a lack of conserved T-cell epitopes between endemic H1N1 and H3N2 influenza viruses and the novel H7N9 virus contributes to this observation. Here we demonstrate that a number of T cell epitopes are conserved between endemic H1N1 and H3N2 viruses and H7N9 virus. Most of these conserved epitopes are from viral internal proteins. The extent of conservation between endemic human seasonal influenza and avian influenza H7N9 was comparable to that with the highly pathogenic avian influenza H5N1. Thus, the ease of inter-species transmission of H7N9 viruses(compared with avian H5N1 viruses) cannot be attributed to the lack of conservation of such T cell epitopes. On the contrary, our findings predict significant T-cell based cross-reactions in the human population to the novel H7N9 virus. Our findings also have implications for H7N9 virus vaccine design.

关键词： H7N9 influenza virus T cell epitope conservation clinical phenotype vaccine immunity

Novel Coronaviruses and Astroviruses in Bats

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Virologica Sinica 2009年第2期24卷 100-104页

作者： Daniel K. W. Chu J. S. malik peiris Leo L. M. Poon State Key Laboratory of Emerging Infectious Diseases Department of MicrobiologyThe University of Hong Kong

Zoonotic transmissions of emerging pathogens from wildlife to human have shaped the history of mankind. These events have also highlighted our poor understanding of microorganisms circulated in wild animals. Coronaviruses and astroviruses,which can be found from a wide range of mammals,were recently detected in bats. Strikingly,these bat viruses are genetically highly diverse and these interesting findings might help to better understand the evolution and ecology of these viruses. The discoveries of these novel bats viruses not only suggested that bats are important hosts for these virus families,but also reiterated the role of bats as a reservoir of viruses that might pose a zoonotic threat to human health.

关键词： Coronavirus Astroviruses Emerging pathogens

Cellular response to influenza virus infection: a potential role for autophagy in CXCL10 and interferon-alpha induction

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Cellular & Molecular Immunology 2010年第4期7卷 263-270页

作者： Anna Hing-Yee Law Davy Chun-Wai Lee Kwok-Yung Yuen malik peiris Allan Sik-Yin Lau Cytokine Biology Group Department of Paediatrics and Adolescent MedicineLi Ka Shing Faculty of MedicineThe University of Hong KongHong KongChina Department of Microbiology Li Ka Shing Faculty of MedicineThe University of Hong KongHong KongChina

Historically,influenza pandemics have arisen from avian influenza *** influenza viruses H5N1 and H9N2 are potential pandemic *** of humans with the highly pathogenic avian influenza H5N1 virus is associated with a mortality in excess of 60%,which has been attributed to dysregulation of the cytokine *** macrophages and epithelial cells infected with some genotypes of H5N1 and H9N2 viruses express markedly elevated cytokine and chemokine levels when compared with seasonal influenza A subtype H1N1 *** mechanisms underlying this cytokine and chemokine hyperinduction are not fully *** the present study,we demonstrate that autophagy,a tightly regulated homeostatic process for self-digestion of unwanted cellular subcomponents,plays a role in cytokine *** is induced to a greater extent by H9N2/G1,in association with cytokine hyperinduction,compared with H1N1 and the novel pandemic swine-origin influenza A/H1N1 *** 3-methyladenine to inhibit autophagy and small interfering RNA to silence the autophagy gene,Atg5,we further show that autophagic responses play a role in influenza virus-induced CXCL10 and interferon-a expression in primary human blood *** results provide new insights into the pathogenic mechanisms of avian influenza viruses.

关键词： autophagy CXCL10 influenza interferon

A novel luciferase immunosorbent assay performs better than a commercial enzyme-linked immunosorbent assay to detect MERS-CoV specific IgG in humans and animals

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Biosafety and Health 2019年第3期1卷 134-143页

作者： Wenling Wang Tianyu Wang Yao Deng Peihua Niu Ruhan A Jincun Zhao malik peiris Shixing Tang Wenjie Tan MHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChina CDCBeijing 102206China Guangdong Provincial Key Laboratory of Tropical Disease Research Department of EpidemiologySchool of Public HealthSouthern Medical UniversityGuangzhou 510515China State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory DiseaseThe first Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510182China School of Public Health Li Ka Shing Faculty of MedicineThe University of Hong KongPokfulamHong Kong Special Administrative Region Center for Biosafety Mega-science Chinese Academy of SciencesBeijing 100101China

The Middle East respiratory syndrome(MERS)is a lethal zoonosis caused by MERS coronavirus(MERS-CoV)and poses a significant threat to public health ***,a rapid,sensitive,and specific serologic test for detecting anti-MERS-CoV antibodies in both humans and animals is urgently needed for the successful management of this ***,we evaluated various novel luciferase immunosorbent assays(LISA)based on nucleocapsid protein(NP)as well as fragments derived from spike protein(S)including subunit 1(S1),N terminal domain(NTD),receptorbinding domain(RBD)and subunit 2(S2)of S for the detection of MERS-CoV-specific *** proteins,including nanoluciferase(NLuc)and various fragments derived from the NP or S protein of MERS-CoV,were expressed in human embryonic kidney 293 T *** that detected anti-MERS-CoV IgG were further developed using cell lysates expressing various fusion *** of human or animal samples infected with MERS-CoV were used to analyze the sensitivity and specificity of various LISAs in reference to a MERS-CoV RT-PCR,commercial S1-based ELISA,and pseudovirus particle neutralization test(ppNT).Our results showed that the S1-,RBD-,and NP-LISAs were more sensitive than the NTD-and S2-LISAs for the detection of anti-MERS-CoV ***,the S1-,RBD-,and NP-LISAs were more sensitive(by at least 16-fold)than the commercially available ***,the S1-,RBD-,and NPLISA specifically recognized anti-MERS-CoV IgG and did not cross-react with samples derived from other human CoV(OC43,229E,HKU1,NL63)-infected *** importantly,these LISAs proved their applicability and reliability for detecting anti-MERS-CoV IgG in samples from camels,monkeys,and mice,among which the RBD-LISA exhibited excellent *** results of this study suggest that the novel MERS-CoV RBD-and S1-LISAs are highly effective platforms for the rapid and sensitive detection of anti-MERS-CoV IgG in human and animal *** assays have

关键词： Luciferase immunosorbent assay(LISA) MERS-CoV Serological IgG detection Samples of humans and animals

H5N1禽流感病毒继续流行和变异——H5N1病毒正以不可预测的方式继续发生变异，对鸟类和人类构成严重威胁

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海峡预防医学杂志 2008年第3期14卷 95-96,F0003页

作者： Robert G. Webster Yi Guan malik peiris 修文琼（译）张拥军（校）不详福建省疾病预防控制中心福州350001

高致病H5N1流感病毒的变体从亚洲传播到印度、欧洲和非洲,并持续在中国的东南部进化,产生了新的突变体,研究者担心由此引发病毒传播到各地及在全球野生鸟类中流行。到目前为止,在感染H5N1病毒的250例中,半数以上死亡。为控制病毒传播,... 详细信息

高致病H5N1流感病毒的变体从亚洲传播到印度、欧洲和非洲,并持续在中国的东南部进化,产生了新的突变体,研究者担心由此引发病毒传播到各地及在全球野生鸟类中流行。到目前为止,在感染H5N1病毒的250例中,半数以上死亡。为控制病毒传播,亚洲有2亿多的家禽被宰杀。HSN1病毒打破了有关流感病毒的一些观念,包括何种鸟可以平安地传播病毒,何种鸟中病毒能致病和致死,以及何种猫可被感染并且能够传播病毒等。一旦在一个地区内一株特别的流感病毒在鸟类中广泛传播时,对家禽扑杀的控制措施应当扩大,同时配合使用疫苗。

关键词：禽流感病毒 H5N1 流行病学鸟类

Broad Reactive Monoclonal Antibodies Provided Cross Protection against H5N1 Avian Influenza Infection in Vivo

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Broad Reactive Monoclonal Antibodies Provided Cross Protecti...

Antibody and T cell responses against wild-type and Omicron SARS-CoV-2 after third-dose BNT162b2 in adolescents

第3届新生病毒性疾病控制学术研讨会

作者：史维国陈毅歆秦堃李国强张军杜海莲吴文翰 J.S.malik peiris 管轶陈鸿霖夏宁邵国家传染病诊断试剂与疫苗工程技术研究中心厦门大学新发传染病国家重点实验室香港大学 International Institute of Infection and Immunity Shantou UniversityShantouChina International Institute of Infection and Immunity Shantou UniversityShantouChina.

With the intention of generating cross-reactive monoclonal antibodies(mAbs),we successively immunized mice with antigenically distinct strains of H5N1 viruses. Large number of mAbs were generated and selected by HI an...

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Signal Transduction and Targeted Therapy 2023年第1期8卷 266-275页

作者： Xiaofeng Mu Carolyn A.Cohen Daniel Leung Jaime S.Rosa Duque Samuel M.S.Cheng Yuet Chung Howard H.W.Wong Amos M.T.Lee Wing Yan Li Issan Y.S.Tam Jennifer H.Y.Lam Derek H.L.Lee Sau Man Chan Leo C.H.Tsang Karl C.K.Chan John K.C.Li Leo L.H.Luk Sara Chaothai Kelvin K.H.Kwan Nym Coco Chu Masashi Mori Trushar Jeevan Ahmed Kandeil Richard JWebby Wenwei Tu Sophie A.Valkenburg malik peiris Yu Lung Lau Department of Paediatrics and Adolescent Medicine The University of Hong KongHong KongChina HKU-Pasteur Research Pole School of Public HealthThe University of Hong KongHong KongChina School of Public Health The University of Hong KongHong KongChina Research Institute for Bioresources and Biotechnology Ishikawa Prefectural UniversityNonoichiJapan Department of Infectious Diseases St Jude Children’s Research HospitalMemphisTNUSA Department of Microbiology and Immunology Peter Doherty Institute for Infection and ImmunityUniversity of MelbourneMelbourneVICAustralia Centre for Immunology&Infection C2i Hong KongChina

The high effectiveness of the third dose of BNT162b2 in healthy adolescents against Omicron BA.1 has been reported in some studies,but immune responses conferring this protection are not yet *** this analysis,our study(NCT04800133)aims to evaluate the humoral and cellular responses against wild-type and Omicron(BA.1,BA.2 and/or BA.5)SARS-CoV-2 before and after a third dose of BNT162b2 in healthy *** 5 months after 2 doses,S IgG,S IgG Fc receptor-binding,and neutralising antibody responses waned significantly,yet neutralising antibodies remained detectable in all tested adolescents and S IgG avidity increased from 1 month after 2 *** antibody responses and S-specific IFN-γ^(+)and IL-2^(+)CD8^(+)T cell responses were significantly boosted in healthy adolescents after a homologous third dose of *** to adults,humoral responses for the third dose were non-inferior or superior in *** S-specific IFN-γ^(+)and IL-2^(+)CD4^(+)and CD8^(+)T cell responses in adolescents and adults were comparable or ***,after 3 doses,adolescents had preserved S IgG,S IgG avidity,S IgG FcγRIIIa-binding,against Omicron BA.2,as well as preserved cellular responses against BA.1 S and moderate neutralisation levels against BA.1,BA.2 and *** from 100 and 96%of adolescents tested at 1 and 5 months after two doses could also neutralise *** study found high antibody and T cell responses,including potent cross-variant reactivity,after three doses of BNT162b2 vaccine in adolescents in its current formulation,suggesting that current vaccines can be protective against symptomatic Omicron disease.

关键词： doses neutral protective