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Genetic map construction and functional characterization of genes within the segregation distortion regions(SdRs)in the F_(2:3) populations derived from wild cotton species of the d genome

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Journal of Cotton Research 2020年第4期3卷 278-296页

作者： KIRUNGU joy Nyangasi MAGWANGA Richard Odongo SHIRAKU Margaret Linyerera LU Pu MEHARI Teame Gereziher XU Yuanchao HOU Yuqing AGONG Stephen Gaya ZHOU Yun CAI Xiaoyan ZHOU Zhongli WANG Kunbo LIU Fang State Key Laboratory of Cotton Biology/Institute of Cotton Research Chinese Academy of Agricultural ScienceAnyang 455000HenanChina School of Biological PhysicalMathematics and Actuarial Sciences(SBPMAS)Jaramogi Oginga Odinga University of Science and Technology(JOOUST)210-40601BondoKenya School of Life Science Henan University/State Key Laboratory of Cotton Biology/Henan Key Laboratory of Plant Stress BiologyKeifang 475004HenanChina School of Agricultural Sciences Zhengzhou UniversityZhengzhou 450001HenanChina.

Background:Segregation distortion(Sd)is a common phenomenon among stable or segregating populations,and the principle behind it still puzzles many *** F2:3 progenies developed from the wild cotton species of the d genomes were used to investigate the possible plant transcription factors within the segregation distortion regions(SdRs).A consensus map was developed between two maps from the four d genomes,map A derived from F2:3 progenies of Gossypium klotzschianum and *** while Map B from *** and *** F2:3 *** each map,188 individual plants were ***:The consensus linkage map had 1492 markers across the 13 linkage groups with a map size of 1467.445 cM and an average marker distance of 1.0370 *** d502 had the highest percentage of Sd with 58.6%,followed by Chromosome d507 with 47.9%.Six thousand and thirty-eight genes were mined within the SdRs on chromosome d502 and d507 of the consensus *** chromosome d502 and d507,2308 and 3730 genes were mined,respectively,and were found to belong to 1117 gourp out of which 622 groups were common across the two ***,genes within the top 9 groups related to plant resistance genes(R genes),whereas 188 genes encoding protein kinase domain(PF00069)comprised the largest *** analysis of the dominant gene group revealed that 287 miRNAs were found to target various genes,such as the gra-miR398,gramiR5207,miR164a,miR164b,miR164c among others,which have been found to target top-ranked stress-responsive transcription factors such as NAC ***,some of the stress-responsive cis-regulatory elements were also ***,RNA profiling of the genes from the dominant family showed that higher numbers of genes were highly upregulated under salt and osmotic stress conditions,and also they were highly expressed at different stages of fiber ***:The results indicated the critical role of the SdRs in the evolution of the k

关键词： Genetic map Segregation distortion region Cis-regulatory elements Genes miRNA

Application of tissue ultrasound palpation system （TUPS） in objective scar evaluation

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Application of tissue ultrasound palpation system （TUPS） i...

Frequency of Monthly Ejaculation at Sexual Intercourse, dNA Fragmentation Index and Fertilization Rate among Sperm donors and Fertility-Challenged Men in Sub-Saharan Africa: Paper 1

第八届全国烧伤外科学年会

作者： joy C.M. Lau Cecilia W.P.Li-Tsang department of Rehabilitation Sciences The Hong Kong Polytechnic University

＜正＞One of the characteristics of hypertrophic scarfing is its raised *** maturation often results in increased *** rely on subjective observation and palpation to document scar thickness. The result of these subjective assessments may reflect onlythe superficial scar thickness but is unable to measure the whole scar volume and thickness under the skin *** of scarthickness using ultrasound imaging has been previously reported,but has not been commonly used in clinics due to its complex operationmethod and high *** this study,we have adopted a newly developed and user-friendly Tissue ultrasound palpation system （TUPS） for theassessment of scar *** consists of a finger size palpation probe,connected to an ultrasound transducer and an in-series load cell to measure the thickness of the soft tissue over the human *** method of operation is easy and it can be used to measure skin thickness on various parts of the body,thus reflecting the skin *** reliability of the TUPS in clinical application was tested on 30 subjects with a hypertrophic scar at a local *** raters implemented two assessments on each subject to study its test-retest and inter-rater *** was then used to assess 100 subjects with various severity of hypertrophic scar caused by trauma,scald,burn or *** were assessed using TUPS as well as the Vancouver Scar Scale （VSS） for rating scar thickness,pliability,pigmentation and *** mixed intra-class correlation showed a high test-retest reliability with Intraclass Correlation Coefficient （ICC）=0.98 and inter-rater reliability ICC=*** positive correlations with VSS thickness score and VSS total score r=0.34 （p＜0.05） and 0.42 （p＜ 0.05）,respectively.A significant difference between two scar type groups （50 burn scald scars and 50 surgical scars） was demonstrated （d.f.=52.94,t=3.99,p＜0.01）.TUPS was proved to have high inter-rater,test-rete

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Open Journal of Urology 2019年第11期9卷 181-194页

作者： Abayomi B. Ajayi Bamgboye M. Afolabi Victor d. Ajayi Ifeoluwa O. Oyetunji Adedamilola Atiba Seun Saanu Ayodeji T. Adeoye joy Ehichioya Ibukun I. Ayelehin Nordica Fertility Center Lagos Nigeria Health Environment and Development Foundation Lagos Nigeria

Introduction: In humans, the ideal ejaculation frequency depends on age, exercise and sexual potential. Natural ejaculation frequency balances the testosterone levels in the body. Materials and Methods: Semi-structured questionnaire was used to collect information from subjects. Semen samples were collected from sperm donors and sub-fertile men who presented for infertility challenges. Processing and analysis of semen samples were done according to World Health Organization guidelines. Sperm dNA fragmentation was evaluated using the Halosperm®?kit. Results: A total of 114 subjects, including 19 sperm donors and 95 sub-fertile males were studied. There was a significant difference (t =?−5.96, P-value = 0.00001) in the mean [±sd] age of sperm donors (30.8 [8.1]) and that of sub-fertile men (42.3 [76]). There was a significant difference (t=?−4.10, P-value = 0.0005) in the mean monthly ejaculation during sexual intercourse (MESI) among sperm donors with dNA fragmentation index −2.20, P-value = 0.02) in MESI among sub-fertile men aged ≥40 years (8.9 [4.6]) than among those aged −0.67, SE = 0.28, t = −2.40, P-value = 0.02, 95% CI: −1.24,?−0.10). Conclusion: In men did not worsen the dFI. A higher number of professionals such as doctors, lawyers and engineers, reported lower monthly frequency of sexual ejaculations compared to entrepreneurs. Entrepreneurs and non-smokers had the highest frequencies of MESI.

关键词： Monthly Sexual Ejaculation Rate dNA Fragmentation Index Male Infertility Age Group Body Mass Index Social Habits Black Africans

Pyospermia in Men Seeking Assisted Reproduction Technology

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Open Journal of Urology 2018年第4期8卷 93-107页

作者： Abayomi B. Ajayi Bamgboye M. Afolabi Victor d. Ajayi Ifeoluwa O. Oyetunji Oluwafunmilola R. Biobaku Adedamilola Atiba joy Ehichioya Ibukun Ayelehin Ayodeji Adeoye Temilade Adesida Nordica Fertility Center Lagos Nigeria Health Environment and Development Foundation Lagos Nigeria

Introduction: Pyospermia is often detected in semen, especially when investigating for male infertility. Pyospermia, in a significantly large number is often correlated with inferior sperm parameters and reduced fertility. Pyospermia may be a factor of a fundamental genitourinary infection. This study assessed the relationship between pus cells and semen parameters in infertile Nigerian males. Subjects and Methods: A cross-sectional descriptive study was carried out at Nordica Fertility Center, Lagos, Nigeria, from 2004 to 2009. A total of 907 subjects were analyzed. Subjects ages (years) were categorized into ≤30, 30.1 - 35, 35.1 - 40, 40.1 - 45, 45.1 - 50 and >50, BMI into Results: Leucocytopenia of ≥3 per high power field (/hpf) occurred in 67 (7.4%) men. The overall means (±sd) of pus cells was 1.4 (0.9), 1.4 (0.7), 1.4 (0.7) and 1.6 (1.6) per high power field in all, normospermia, oligoospermic and azoospermic clusters of patients respectively. Mean (±sd) pus cells in semen was 6.0 (4.0), the highest, among obese (BMI ≥ 30) azoospermic patients. The proportion of subjects with pyospermia of 1 - 2 was highest (323/346, 93.3%) among oligoospermic patients while that with pyospermia of ≥3 was highest (10/109, 9.2%) among azoospermic patients. Obese azoospermic patients were 1.8 times as likely to have ≥3/hpf pyospermia compared to normal normospermic patients (x2 = 0.05, P-value = 0.51, OR = 1.82, 95% CI: 0.30, 11.02). Mean progressive motility (%) was significantly reduced (t = 1.95, P-value = 0.03) among patients with ≥3/hpf pyospermia (33.75 ± 19.23) compared to those with 1 - 2/hpf pyospermia (38.58 ± 21.63). Patients with sperm oval head ≤ 30% had significantly higher pyospermia (t = 2.22, P-value = 0.013) than those with sperm oval head of >30%. Conclusion: Elevated pus cell counts were observed among obese azoospermic men, those with reduced sperm progressive motility and those with lower count of sperms with oval head morphology. Clinicians should consider

关键词： Pyospermia Male Infertility Progressive Motility Oval Head Nigeria

用于高速A/d转换器的GaAs HJBT和硅双极工艺的比较

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微电子学 1989年第6期19卷 79-86,17页

作者： Andrew K.joy 肖辉杨

本文研究了在全并行A/d转换器领域中GaAlAs/GaAs异质结双极晶体管(HJBT)工艺的应用前景。为了预测HJBT的前景,与用现代硅工艺可达到的最好性能水平进行了比较。用每一种工艺研制了最佳的锁存比较器,并且进行了SPICE模拟,以便决定其能达... 详细信息

本文研究了在全并行A/d转换器领域中GaAlAs/GaAs异质结双极晶体管(HJBT)工艺的应用前景。为了预测HJBT的前景,与用现代硅工艺可达到的最好性能水平进行了比较。用每一种工艺研制了最佳的锁存比较器,并且进行了SPICE模拟,以便决定其能达到的最大采样率和大信号模拟带宽。在我们进行上述两种工艺的比较时,有这样的一些工艺模式可选用,一种是近期开发阶段的标准的1μm双极工艺与4μmHJBT工艺;另一种是早期开发阶段改进的1μm硅双极工艺与2.5μmHJBT工艺。这样使得随时间流逝的性能改善趋势亦可进行比较。

关键词： A/d转换器 GaAs异质结双极晶体管

Atherosclerosis imaging using 3d black blood TSE SPACE vs 2d TSE

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World Journal of Radiology 2014年第5期6卷 192-202页

作者： Stephanie K Wong Motunrayo Mobolaji-Iawal Leron Arama joy Cambe Sylvia Biso Nadia Alie Zahi A Fayad Venkatesh Mani Translational and Molecular Imaging Institute Icahn School of Medicine at Mt. Sinai New York NY 10029United States department of Radiology Icahn School of Medicine at Mt. Sinai New York NY 10029 United States Zena and Michael A Wiener Cardiovascular Institute Department of Medicine Marie-Josée and Henry R Kravis Cardiovascular Health Center Icahn School of Medicine at Mt.Sinai New York NY 10029 United States

AIM: To compare 3d Black Blood turbo spin echo(TSE)sampling perfection with application-optimized contrast using different flip angle evolution(SPACE) vs 2d TSE in evaluating atherosclerotic plaques in multiple vascular territories. METHOdS: The carotid, aortic, and femoral arterial walls of 16 patients at risk for cardiovascular or atherosclerotic disease were studied using both 3d black blood magnetic resonance imaging SPACE and conventional 2d multi-contrast TSE sequences using a consolidated imaging approach in the same imaging session. Qualitative and quantitative analyses were performed on the images. Agreement of morphometric measurements between the two imaging sequences was assessed using a two-sample t-test, calculation of the intra-class correlation coefficient and by the method of linear regression and Bland-Altman analyses. RESULTS: No statistically significant qualitative differences were found between the 3d SPACE and 2d TSE techniques for images of the carotids and aorta. For images of the femoral arteries, however, there were statistically significant differences in all four qualitative scores between the two techniques. Using the current approach, 3d SPACE is suboptimal for femoral imaging. However, this may be due to coils not being optimized for femoral imaging. Quantitatively, in our study, higher mean total vessel area measurements for the 3d SPACE technique across all three vascular beds were observed. No significant differences in lumen area for both the right and left carotids were observed between the two techniques. Overall, a significant-correlation existed between measures obtained between the two approaches. CONCLUSION: Qualitative and quantitative measurements between 3d SPACE and 2d TSE techniques are comparable. 3d-SPACE may be a feasible approach in the evaluation of cardiovascular patients.

关键词： Atherosclerosis Carotid artery plaque Aorta Femoral artery Magnetic resonance imaging

Frequency of Monthly Ejaculation at Sexual Intercourse, dNA Fragmentation Index and Fertilization Rate among Sperm donors and Fertility-Challenged Men in Sub-Saharan Africa: Paper 2

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Open Journal of Urology 2020年第3期10卷 60-78页

作者： Abayomi B. Ajayi Bamgboye M. Afolabi Victor d. Ajayi Ifeoluwa O. Oyetunji Adedamilola Atiba Seun Saanu Ayodeji T. Adeoye joy I. Ehichioya Ibukun I. Ayelehin Nordica Fertility Center Lagos Nigeria Health Environment and Development Foundation Lagos Nigeria

Introduction: Heterogenous sexual intercourse often produces a powerful orgasm that stimulates the pituitary function. Studies have suggested ejaculation frequency based on age. Materials and Methods: Semi-structured questionnaires were used to collect information from subjects. Semen samples were collected from sperm donors and sub-fertile men who presented for infertility challenges. Processing and analysis of semen samples were done according to World Health Organization (WHO) guidelines. Sperm deoxyribonucleic acid (dNA) fragmentation was evaluated using the Halosperm kit. Results: Among sperm donors, those aged χ2 = 0.09, P-value = 0.81, OR = 3.2, 95% CI: 0.23, 45.19) while among sub-fertile men those aged ≥40 years were approximately 2.3 times more likely to have very low frequency of MESI compared to aged χ2 = 2.11, P-value = 0.51, OR = 2.27, 95% CI: 0.74, 9.92). The frequency of all categories of MESI was highest among sub-fertile men aged χ2 = 0.03, P-value = 0.57, OR = 2.42, 95% CI: 0.41, 14.46). Sub-fertile men aged ≥40 with diastolic hypertension (≥90 mmHg) were approximately 6 times more likely to have a low (6 - 10) frequency of MESI compared to those aged χ2 = 1.97, P-value = 0.16, OR = 6.22, 95% CI: 0.69, 55.77). Overall mean fertilization rate was significantly higher (t-test = 2.44, P-value = 0.01) among sub-fertile men aged Conclusion: Cigarette smoking, more than alcohol consumption, appears to be a major factor that significantly affects the frequency of MESI among sub-fertile men in all age groups. Frequency of MESI negatively but insignificantly correlated with systolic blood pressure.

关键词： Monthly Ejaculation at Sexual Intercourse Systolic and diastolic Blood Pressures dNA Fragmentation Index Fertilization Rates Social Habits Sub-Fertile Males Sub-Sahara Black Africans

Evaluation of Sperm dNA Fragmentation amongst Infertile Black Africans. A Nigerian Study

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Open Journal of Urology 2018年第11期8卷 297-316页

作者： Abayomi B. Ajayi Bamgboye M. Afolabi Victor d. Ajayi Ifeoluwa O. Oyetunji Adedamilola Atiba Seun Saanu Ayodeji T. Adeoye Temilade E. Adeshida joy Ehichioya Ibukun I. Ayelehin Nordica Fertility Center Lagos Nigeria Health Environment and Development Foundation Lagos Nigeria

Background: Male infertility is approaching an epidemic proportion. Almost 50% of all cases of infertility may be associated with a male factor. The diagnostic usefulness of sperm dNA integrity is now accessible as an additional tool to Seminal Fluid Analysis. Objective: To assess sperm dNA fragmentation index (SdFI) in male infertility and its relationship with obesity, alcohol consumption and cigarette smoking among infertile Nigerians. Patients and Methods: Patients who presented for infertility at three health facilities of Nordica Fertility Center in Lagos, Asaba and Abuja cities in Nigeria. STATA 13 was used for student’s t-test to compare the means of continuous variables among smokers and non-smokers and among alcohol consumers and non-consumers. Linear regression analysis was employed to assess the correlation between SdFI as dependent variable and some independent variables. Results: There was no significant difference in the SdFI of men aged −1.80, P-value = 0.04) than that of obese men (30.2%). Obese men were 2.12 times as likely to have SdFI ≥25% compared to normal weight men (χ2-2.16, P-value = 0.14, OR = 2.12, 95% CI: 0.77, 5.80). Mean SdFI of men who consume alcohol (37.1%) was significantly higher (t = −1.97, P-value = 0.03) than that of those who did not consume alcohol. Although Pearson’s correlation matrix (r) indicated that sperm dNA fragmentation index was positively correlated with history of infertility (r = 0.01), groin surgery (r = 0.04), mumps (r = 0.04) and sexually transmitted illness (r = 0.04), however the degree of correlation was not significant (P-value ≥ 0.5) in each case. Conclusion: This is the first report in Black Africa that describes a correlation between sperm dNA integrity, as measured by the halo test and age, BMI and alcohol consumption. Men with normal BMI were more likely to have excellent to good SdFI and hence good fertility potential. data from this study indicate that the infertile men had significan

关键词： Sperm Fragmentation Index Obesity Alcohol Consumption Male Infertility Assisted Reproduction Technology Black Africans

Validation of an objective scar pigmentation measurement by using a spectrocolorimeter

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Validation of an objective scar pigmentation measurement by ...