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A Method for Astral Microtubule Tracking in Fluorescence Images of Cells Doped with Taxol and Nocodazole

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Advances in Molecular Imaging 2019年第4期9卷 60-86页

作者： Marilena Varrecchia joshua levine Gabriella Olmo Marco Grangetto Marta Gai Ferdinando Di Cunto Department of Automatics and Computer Science Politecnico di Torino Torino Italy

In this paper, we describe an algorithm that performs automatic detection and tracking of astral microtubules in fluorescence confocal images. This sub-population of microtubules only exists during and immediately before mitosis and aids in the spindle orientation by connecting it to the cell cortex. Anomalies in their dynamic behaviour play a causal role in many diseases, such as development disorders and cancer. The main novelty of the proposed algorithm lies in the fact it provides a fully automated estimation of parameters related to microtubule dynamic instability (growth velocity, track length and track lifetime), and helps in understanding the effects of intermediate drug concentrations. Its performance has been objectively assessed using publicly available synthetic data and largely employed metrics. Moreover, we present experiments addressing cell cultures doped with different concentrations of taxol and nocodazole. Such drugs are known to suppress the microtubule dynamic instability, but their effects at intermediate concentrations are not completely assessed. The algorithm has been compared with other state-of-the-art approaches, tested on consistent real datasets. The results are encouraging in terms of performance, robustness and simplicity of use, and the algorithm is now routinely employed in our Department of Molecular Biotechnology.

关键词： Medical Diagnostic Imaging Fluorescence Confocal Microscopy Image Segmentation Microtubules

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芬兰模式

疯狂英语（阅读版） 2011年第9期 10-13页

作者： joshua levine 方金凤

在芬兰赫尔辛基市内被称为“里东区”的贫民区里,有一所卡拉提综合学校,一群9岁的孩子们正在学校的一块空地上进行课外教学活动。他们背靠背坐着,用树枝、松果、石头和浆果在地上摆出各种形状。他们要用几何术语来描述自己摆出来的这些... 详细信息

在芬兰赫尔辛基市内被称为“里东区”的贫民区里,有一所卡拉提综合学校,一群9岁的孩子们正在学校的一块空地上进行课外教学活动。他们背靠背坐着,用树枝、松果、石头和浆果在地上摆出各种形状。他们要用几何术语来描述自己摆出来的这些形状,然后让背面看不见那些形状的孩子们来猜。“这是一种完全不同的数学学习方式,不是用笔在纸上画,而是通过这种形式将几何概念以最直接的方式印在孩子们的大脑里。”

关键词：芬兰课外教学活动综合学校几何概念赫尔辛基学习方式孩子形状

Exotic parrots breeding in urban tree cavities: nesting requirements, geographic distribution, and potential impacts on cavity nesting birds in southeast Florida

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Avian Research 2019年第4期10卷 459-468页

作者： joshua M.Diamond Michael S.Ross Florida International University Earth & Environment

Background:Exotic parrots have established breeding populations in southeast Florida,including several species that nest in tree *** aimed to determine the species identity,nest site requirements,relative nest abundance,geographic distribution,and interactions of parrots with native cavity-nesting bird ***:We searched Miami-Dade County,Florida,and nearby areas for natural cavities and holes excavated by woodpeckers,recording attributes of potential nest *** inspected all cavities with an elevated video inspection system to determine occupancy by parrots or other *** mapped nearly 4000 citizen science observations of parrots in our study area corresponding to our study period,and used these to construct range maps,comparing them to our nesting ***:Not all parrots reported or observed in our study area were actively *** parrots were observed at tree cavities,which previous studies have suggested is evidence of reproduction,but our inspections with an elevated video inspection system suggest they never initiated nesting *** parrot species did successfully nest in tree cavities,Red-masked Parakeets(Psittacara erythrogenys)and Orange-winged Parrots(Amazona amazonica)being the most common(n=7 and 6 nests,respectively).These two parrots had similar nesting requirements,but Orange-winged Parrots use nests with larger entrance holes,which they often *** analysis of nests combined with citizen science data indicate that parrots are limited to developed *** most common parrots were less abundant cavity nesters than the native birds which persist in Miami’s urban areas,and far less abundant than the invasive European Starling(Sturnus vulgaris).Conclusions:Exotic parrots breeding elsewhere in the world have harmed native cavity-nesting birds through interference competition,but competitive interference in southeast Florida is minimized by the urban affinities of parrots in this ***

关键词： Amazona Cavity nest webs Invasive species Parakeets Psittacara Urban ecology

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从独乐乐到众乐乐:管理跨年龄层团队

科学新闻 2018年第11期 79-83页

作者： Alaina G.levine 赵红蕾

科研团队比以往任何时候都更加多样化,而且往往是由不同年龄层的人员组成的。了解如何根据不同年龄层的需要调整管理风格,可以确保提高团队的生产力、创造力以及协作能力。41岁的joshua Pearce是位于休斯顿的密歇根理工大学材料科学与... 详细信息

科研团队比以往任何时候都更加多样化,而且往往是由不同年龄层的人员组成的。了解如何根据不同年龄层的需要调整管理风格,可以确保提高团队的生产力、创造力以及协作能力。41岁的joshua Pearce是位于休斯顿的密歇根理工大学材料科学与工程系的教授,属于X一代(Generation X,20世纪60年代到70年代初出生的美国人)的一

关键词：年龄层移动设备

美国胃食管反流病患者的门诊经历:对1998—2001年全国非卧床患者医疗护理调查的分析

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世界核心医学期刊文摘（胃肠病学分册） 2006年第3期2卷 14-14页

作者： Niemcryk S.J. joshua-Gotlib S. levine D.S. 陈云茹 AstraZeneca LP Delaware 3C-112 1800 Concord Pike Wilmington DE 19850-5437 United States

This study documents the number of ambulatory visits associated with gastroesophageal reflux disease (GERD) in the United States. Sample data from nearly 80,000 patients captured by the National Ambulatory Medical Care Survey (NAMCS; 1998-2001) were analyzed. Basic demographics of patients with GERD and factors associated with each visit were assessed. Approximately 38.53 million of 2.653 billion adult outpatient visits made in the United States during the study period were GERD-related. GERD-related visits increased by 46.5%from 1998 to 2001. Most GERD-related visits were by women (54.7%) with an average age of 56.0 years, compared with patients without GERD, who were even more likely to be women (62.2%) and younger (52.6 years). Patients with GERD were more likely to have multiple reasons (50.5%) and multiple diagnoses (79.3%) per medical visit versus non-GERD patients (37.6%and 48.4%, respectively). Utilization of data from the NAMCS reveals that GERD-related visits increased annually during the study period. Patients with GERD are more likely to see a physician if they have concomitant medical conditions, making GERD a condition that is very likely untreated in a high percentage of individuals.

关键词：胃食管反流卧床患者统计学资料相关性因素检查次数内科医师门诊检查