检索结果-南通市图书馆

四川地区鸡源产气荚膜梭菌的分离鉴定与基因分型

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国人兽共患病学报 2009年第8期25卷 737-740,786页

作者：倪学勤宋振银曾东郑晓丽 joshua Gong 四川农业大学动物医学院动物微生态研究中心雅安625014 动物疫病与人类健康四川省重点实验室雅安625014 四川农业大学资源环境学院雅安625014 Food Research Program Agriculture and Agri-Food Canada

目的检测四川地区健康鸡群产气荚膜梭菌的分布状况。方法从四川地区规模化鸡场采集新鲜粪便样品,按产气荚膜梭菌α、β、ε、ι毒素基因cpa、cpb、etx及iA序列,设计针对4种毒素基因的4对特异引物,应用多重PCR方法对产气荚膜梭菌进行基... 详细信息

目的检测四川地区健康鸡群产气荚膜梭菌的分布状况。方法从四川地区规模化鸡场采集新鲜粪便样品,按产气荚膜梭菌α、β、ε、ι毒素基因cpa、cpb、etx及iA序列,设计针对4种毒素基因的4对特异引物,应用多重PCR方法对产气荚膜梭菌进行基因分型。最后利用重复序列PCR进一步做亚型分析。结果从150份样品中分离到8株(5.3%)产气荚膜梭菌,多重PCR都只扩增出α条带,毒素基因分型结果均为A型。ERIC-PCR、REP-PCR图谱显示两者均适用于产气荚膜梭菌亚型分析,ERIC-PCR是一种更简便、快速和有效的分子流行病学调查方法。结论四川地区健康鸡群中存在的产气荚膜梭菌主要是A型。

关键词：产气荚膜梭菌多重PCR 基因分型鸡

Social rank and cortisol among female rhesus macaques(Macaca mulatta)

在线全文

学校读者我要写书评

暂无评论

Zoological Research 2013年第2期34卷 42-49页

作者： Dong-Dong QIN joshua Dominic Rizak Xiao-li FENG Xun-Xun CHU Shang-Chuan YANG Chun-Lu li Long-Bao LV Yuan-Ye MA Xin-Tian HU Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences&Yunnan Province Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China University of Chinese Academy of Sciences Beijing 100049China Institute of Psychology Chinese Academy of SciencesBeijing 100101China Kunming Primate Research Center Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China State Key Laboratory of Brain and Cognitive Sciences Institute of BiophysicsChinese Academy of SciencesBeijing 100101China

In animal societies,some stressful events can lead to higher levels of physiological *** stressors,like social rank,also predict an increased vulnerability to an array of ***,the physiological relationship between social rank and stress varies between different species,as well as within groups of a single *** example,dominant individuals are more socially stressed at times,while at other times it is the subordinate ones who experience this ***,these variations make it difficult to assess disease vulnerability as connected to social *** order to learn more about how physiological rank relationships vary between groups of a single species,cortisol measurements from hair samples were used to evaluate the effects of dominance rank on long-term stress levels in despotic and less stringent female rhesus macaque hierarchal *** despotic groups,cortisol levels were found not to be correlated with social rank,but a negative correlation was found between social rank and cortisol levels in less stringent *** ranking monkeys in less stringent groups secreted elevated levels of cortisol compared to higher ranking *** data suggest that variations in the strictness of the dominance hierarchy are determining factors in rank related stress *** further consideration of nonhuman primate social system diversity and the linear degree of their hierarchies may allow for the development of valid rank-related stress models that will help increase our understanding and guide the development of new therapeutics for diseases related to human socioeconomic status.

关键词： Social rank Hypothalamus-pituitary-adrenal axis Cortisol Female Rhesus macaques

Feeding Biochar to Cows:An Innovative Solution for Improving Soil Fertility and Farm Productivity

在线全文

学校读者我要写书评

暂无评论

Pedosphere 2015年第5期25卷 666-679页

作者： Stephen JOSEPH Doug POW Kathy DAWSON David R.G.MITCHELL Aditya RAWAL James HOOK Sarasadat TAHERYMOOSAVI Lukas VAN ZWIETEN joshua RUST Scott DONNE Paul MUNROE Ben PACE Ellen GRABER Torsten THOMAS Shaun NIELSEN Jun YE Yun liN PAN Genxing li lianqing Zakaria M.SOLAIMAN Discipline of Chemistry University of Newcastle School of Materials Science and Engineering University of New South Wales Electron Microscopy Center AIIMUniversity of Wollongong Institute of Resources Ecosystem and Environment of AgricultureNanjing Agricultural University Marron Brook Farm Warren Catchments Council 52 Bath StManjimup 6258 (Australia) Mark Wainwright Analytical Centre University of New South Wales Wollongbar Primary Industries Institute NSW Department of Primary Industries Institute of Soil Water and Environmental SciencesThe Volcani CenterAgricultural Research Organization Centre for Marine Bio-Innovation School of Biotechnology and Biomolecular SciencesThe University of New South Wales Soil Biology and Molecular Ecology Group School of Earth and Environment (M087)The University of Western Australia

Addition of biochar produced through thermal decomposition of biomass has been seen as a strategy to improve soils and to sequester carbon （C）, but wide scale implementation of the technology requires to devise innovative profitable solutions. To develop biochar utilisation with an integrated system approach, an innovative program was implemented in 2012 on a 53-ha farm in Western Australia to determine the costs and benefits of integrating biochar with animal husbandry and improvement of pastures. Biochar was mixed with molasses and fed directly to cows. The dung-biochar mixture was incorporated into the soft profile by dung beetles. We studied the changes in soil properties over 3 years. Biochar extracted from fresh dung and from the soil to a depth of 40 cm was characterised. A preliminary financial analysis of the costs and benefits of this integrated approach was also undertaken. The preliminary investigation results suggested that this strategy was effective in improving soil properties and increasing returns to the farmer. It was also concluded that the biochar adsorbed nutrients from the cow＇s gut and from the dung. Dung beetles could transport this nutrient-rich biochar into the soil profile. There was little evidence that the recalcitrant component of the biochar was reduced through reactions inside the gut or on/in the soil. Further research is required to quantify the long-term impact of integrating biochar and dung beetles into the rearing of cows.

关键词： animal husbandry biochar C sequestration dung beetles financial benefit pasture

在线全文

学校读者我要写书评

暂无评论

生长因子在眼的发育及眼部疾病调控中的作用

浙江大学学报（医学版） 2022年第5期51卷 613-625页

作者：王晓杰惠琦金子饶凤琴靳磊余丙洁 BANDA joshua 李校堃温州医科大学药学院浙江温州325035

生长因子是一类由多种细胞分泌的活性物质,作为信使调控细胞的迁移、增殖和分化。多种生长因子参与眼组织的发育及眼部疾病的生理、病理过程。血管内皮生长因子、碱性成纤维细胞生长因子等介导糖尿病性视网膜病、脉络膜新生血管、白内... 详细信息

生长因子是一类由多种细胞分泌的活性物质,作为信使调控细胞的迁移、增殖和分化。多种生长因子参与眼组织的发育及眼部疾病的生理、病理过程。血管内皮生长因子、碱性成纤维细胞生长因子等介导糖尿病性视网膜病、脉络膜新生血管、白内障、糖尿病性黄斑水肿以及其他视网膜疾病的发生和发展。神经生长因子、睫状神经营养因子、胶质细胞源性神经营养因子、色素上皮衍生因子、粒细胞集落刺激因子等对视神经损伤有较好的修复作用。生长因子还与近视的发病密切相关,成纤维细胞生长因子、转化生长因子-β、胰岛素样生长因子影响巩膜厚度变化并调控近视的发生与发展。本文综述了生长因子参与眼的发育和眼部病理生理过程的研究进展,旨在揭示生长因子与眼部疾病的关联,为生长因子在眼科领域的应用提供思路。

关键词：生长因子眼的发育眼部疾病眼科学综述

在线全文

学校读者我要写书评

暂无评论

细胞因子类药物在眼科临床的应用进展

浙江大学学报（医学版） 2022年第5期51卷 626-633页

作者：王晓杰惠琦金子饶凤琴余丙洁靳磊 BANDA joshua 李校堃温州医科大学药学院浙江温州325035

血管内皮生长因子、成纤维细胞生长因子、神经生长因子、表皮生长因子、干扰素等细胞生长因子是调控细胞增殖、分化和再生的重要内源性蛋白。以上述生长因子等为靶点开发的生物药物,在老年性黄斑变性、角膜损伤、神经营养性角膜炎等眼... 详细信息

血管内皮生长因子、成纤维细胞生长因子、神经生长因子、表皮生长因子、干扰素等细胞生长因子是调控细胞增殖、分化和再生的重要内源性蛋白。以上述生长因子等为靶点开发的生物药物,在老年性黄斑变性、角膜损伤、神经营养性角膜炎等眼部疾病的治疗中发挥了重要作用,相应药物陆续上市。抗血管内皮生长因子类药物可以控制血管内皮的增生,减轻视网膜组织的水肿和渗出,已经成为老年性黄斑变性和糖尿病视网膜病的主要治疗手段。碱性成纤维细胞生长因子可促进角膜上皮细胞的增殖、分化和迁移,具有加速角膜损伤愈合,降低角膜炎症反应的作用,其中牛碱性成纤维细胞生长因子已用于角膜损伤临床治疗。神经生长因子能促进中枢和外周神经元的生长、发育和分化,加快神经损伤的修复,用于神经营养性角膜炎的治疗,可促进角膜完全愈合。临床上,表皮生长因子衍生物滴眼液可用于角膜上皮损伤的治疗,重组人干扰素可用于眼部病毒性感染疾病的治疗。本文就细胞因子类药物在眼科疾病治疗及新药开发的研究进展进行综述,为拓展细胞因子在眼科临床的应用提供参考。

关键词：成纤维细胞生长因子血管内皮生长因子神经生长因子表皮生长因子干扰素白介素眼科药物综述

Using Novel Statistical Techniques to Accurately Determine the Predictive Dose Range in a Study of Overall Survival after Definitive Radiotherapy for Stage III Non-Small Cell Lung Cancer in Association with Heart Dose

在线全文

学校读者我要写书评

暂无评论

Journal of Cancer Therapy 2021年第9期12卷 505-529页

作者： joshua R. Niska Jiuyun Hu Jing li Michael G. Herman Cameron S. Thorpe Steven E. Schild Mirek Fatyga Department of Radiation Oncology Banner MD Anderson Cancer Center Phoenix Arizona USA School of Computing Informatics and Decision Systems Engineering Tempe Arizona USA School of Industrial and Systems Engineering Georgia Institute of Technology Atlanta Georgia USA Department of Radiation Oncology Mayo Clinic Rochester Minnesota USA Department of Radiation Oncology Mayo Clinic Phoenix Arizona USA

Purpose: Recent studies of radiotherapy (RT) for stage III non-small-cell lung cancer (NSCLC) have associated high dose to the heart with cardiac toxicity and decreased overall survival (OS). We used advanced statistical techniques to account for correlations between dosimetric variables and more accurately determine the range of heart doses which are associated with reduced OS in patients receiving RT for stage III NSCLC. Methods: From 2006 to 2013, 119 patients with stage III NSCLC received definitive RT at our institution. OS data was obtained from institutional tumor registry. We used multivariate Cox model to determine patient specific covariates predictive for reduced overall survival. We examined age, prescription dose, mean lung dose, lung V20, RT technique, stage, chemotherapy, tumor laterality, tumor volume, and tumor site as candidate covariates. We subsequently used novel statistical techniques within multivariate Cox model to systematically search the whole heart dose-volume histogram (DVH) for dose parameters associated with OS. Results: Patients were followed until death or 2.5 to 81.2 months (median 30.4 months) in those alive at last follow up. On multivariate analysis of whole heart DVH, the dose of 51 Gy was identified as a threshold dose above which the dose volume relationship becomes predictive for OS. We identified

关键词： Lung Cancer Cardiac Toxicity Lung Radiation Therapy Non-Small Cell Lung Cancer Radiation Toxicity

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

基于区域三维纹理特征的路面抗滑性能评估

东南大学学报（自然科学版） 2020年第4期50卷 667-676页

作者：彭毅李强战友杨广伟王郴平西南交通大学土木工程学院成都610031 西南交通大学道路工程四川省重点实验室成都610031 School of Civil and Environmental Engineering Oklahoma State UniversityStillwaterOK 74078-5013USA 广东省建筑科学研究院集团股份有限公司广州510500

为实现路面抗滑性能非接触式测量,提出应用新型区域三维纹理特征来表征沥青路面形貌构造并评估路面抗滑性能.使用便携式高分辨率三维激光扫描仪采集不同类型沥青路面纹理数据,同时使用动态摩擦系数测试仪采集其路面抗滑性能数据,并分别... 详细信息

为实现路面抗滑性能非接触式测量,提出应用新型区域三维纹理特征来表征沥青路面形貌构造并评估路面抗滑性能.使用便携式高分辨率三维激光扫描仪采集不同类型沥青路面纹理数据,同时使用动态摩擦系数测试仪采集其路面抗滑性能数据,并分别使用70和15 km/h时的动态摩擦系数值代表高速与低速状态下的路面抗滑性能.通过相关性分析和多元线性回归,发现路面抗滑性能与多个区域三维纹理特征参数的共同作用有关.建立前馈神经网络预测模型,使用多个区域三维纹理特征参数预测高速与低速状态下的路面抗滑性能.结果表明,区域三维纹理特征参数对动态摩擦系数测试仪在70 km/h时测得的路面抗滑性能预测能力为77%,对15 km/h时测得的路面抗滑性能预测能力为69%,证实了区域三维纹理特征参数与路面抗滑性能之间存在非线性联系.

关键词：路面纹理抗滑区域纹理参数神经网络

在线全文

学校读者我要写书评

暂无评论

二维、三维超声技术检测正常胎儿胸腺的研究

中华超声影像学杂志 2011年第1期20卷 53-57页

作者：李凌周启昌邹琳蒲大荣章鸣 joshua Copel Mert Bahtiyar 中南大学湘雅二医院超声科长沙410001 Maternal-Fetal Medicine of Yale University Medical School

目的建立胎儿胸腺二维各径线和三维体积的正常值,并比较胎儿胸腺的二维、三维超声测量.方法采集567例正常胎儿胸腺的二维超声测值,包括最大横径、前后径、上下径、最大横截面积及三维超声体积.结果 567例胎儿中541例胎儿满意获得胎儿... 详细信息

目的建立胎儿胸腺二维各径线和三维体积的正常值,并比较胎儿胸腺的二维、三维超声测量.方法采集567例正常胎儿胸腺的二维超声测值,包括最大横径、前后径、上下径、最大横截面积及三维超声体积.结果 567例胎儿中541例胎儿满意获得胎儿胸腺的最大横径、前后径、上下径和最大横截面积和体积.胎儿胸腺二维各径线、三维体积均随孕周的增加而增加,与孕周均呈直线相关,三维体积与孕周的相关性明显高于二维超声测值（P＜0.05）.结论本研究建立了胎儿胸腺二维超声面积、周长、直径、横径和三维超声体积的正常参考值.三维超声测量胎儿胸腺体积与孕周相关性更高,提示较二维超声测量更为准确.

关键词：超声检查产前胎儿胸腺

Comparative study of the transfection efficiency of commonly used viral vectors in rhesus monkey (Macaca mulatta) brains

在线全文

学校读者我要写书评

暂无评论

Zoological Research 2017年第2期38卷 88-95页

作者： Shi-Hao Wu Zhi-Xing liao joshua D. Rizak Na Zheng lin-Heng Zhang Hen Tang Xiao-Bin He Yang Wu Xia-Ping He Mei-Feng Yang Zheng-Hui li Dong-Dong Qin Xin-Tian Hu Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province Kunming Institute of Zoology Chinese Academy of Sciences Kunming Yunnan 650223 China Nerve System Coding Discipline Group Kunming College of Life Science University of the Chinese Academy of Sciences Kunming Yunnan 650000 China Kunming Primate Research Center Kunming Institute of Zoology Chinese Academy of Sciences Kunming Yunnan 650223 China Department of Anatomy and Histology & Embryology Faculty of Basic Medical Science Kunming Medical University Kunming Yunnan 650500 China CAS Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of Sciences Shanghai 200031 China Center for Excellence in Brain Science Wuhan Institute of Physics and Mathematics Chinese Academy of Sciences Wuhan Hubei 430071 China

Viral vector transfection systems are among the simplest of biological agents with the ability to transfer genes into the central nervous system. In brain research, a series of powerful and novel gene editing technologies are based on these systems. Although many viral vectors are used in rodents, their full application has been limited in non-human primates. To identify viral vectors that can stably and effectively express exogenous genes within non- human primates, eleven commonly used recombinant adeno-associated viral and lentiviral vectors, each carrying a gene to express green or red fluorescence, were injected into the parietal cortex of four rhesus monkeys. The expression of fluorescent cells was used to quantify transfection efficiency. Histological results revealed that recombinant adeno-associated viral vectors, especially the serotype 2/9 coupled with the cytomegalovirus, human synapsin I, or Ca2~/calmodulin-dependent protein kinase II promoters, and lentiviral vector coupled with the human ubiquitin C promoter, induced higher expression of fluorescent cells, representing high transfection efficiency. This is the first comparison of transfection efficiencies of different viral vectors carrying different promoters and serotypes in non-human primates （NHPs）. These results can be used as an aid to select optimal vectors to transfer exogenous genes into the central nervous system of non-human primates.

关键词： Recombinant adeno-associated virus lentivirus Rhesus monkey Central nervous system